Analytical and Bioanalytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies
Anal Bioanal Chem. 2024 Feb 6. doi: 10.1007/s00216-024-05185-0. Online ahead of print.ABSTRACTFatty acids (FAs) have attracted many interests for their pivotal roles in many biological processes. Imbalance of FAs is related to a variety of diseases, which makes the measurement of them important in biological samples. Over the past two decades, mass spectrometry (MS) has become an indispensable technique for the analysis of FAs owing to its high sensitivity and precision. Due to complex matrix effect of biological samples and inherent poor ionization efficiency of FAs in MS, sample preparation including extraction and chemi...
Source: Analytical and Bioanalytical Chemistry - February 6, 2024 Category: Chemistry Authors: Li Yang Jie Yuan Bolin Yu Shuang Hu Yu Bai Source Type: research

Assessing human exposure to pesticides and mycotoxins: optimization and validation of a method for multianalyte determination in urine samples
In this study, an analytical method has been developed for the determination of 30 pesticides and 23 mycotoxins of concern in urine samples. A salting-out liquid-liquid extraction (SALLE) procedure was optimized achieving recoveries between 70 and 120% for almost all the compounds and limits as lower as when QuEChERS was applied. The compounds were then determined by liquid chromatography coupled to triple quadrupole mass spectrometry. Different chromatographic conditions and analytical columns were tested, selecting a Hypersild gold aQ column as the best option. Finally, the method was applied to the analysis of 45 urine ...
Source: Analytical and Bioanalytical Chemistry - February 6, 2024 Category: Chemistry Authors: Jes ús Marín-Sáez Maykel Hern ández-Mesa Jose A Gallardo-Ramos Laura G ámiz-Gracia Ana M Garc ía-Campaña Source Type: research

Evaluation of deep eutectic solvents in the synthesis of molecularly imprinted fibers for the solid-phase microextraction of triazines in soil samples
Anal Bioanal Chem. 2024 Mar;416(6):1337-1347. doi: 10.1007/s00216-024-05164-5. Epub 2024 Feb 3.ABSTRACTNowadays, molecularly imprinted polymers (MIPs) are well established and are considered excellent materials for performing selective extractions. However, with the progressive implementation of the principles of green chemistry, it is necessary to find greener alternatives for both the synthesis and further use of MIPs in sample preparation. Accordingly, in the present work, different deep eutectic solvents (DES, both hydrophilic and hydrophobic), as an alternative to conventional organic solvents (i.e., toluene), were ev...
Source: Analytical and Bioanalytical Chemistry - February 3, 2024 Category: Chemistry Authors: Alexia Monnier Myriam D íaz-Álvarez Esther Turiel Antonio Mart ín-Esteban Source Type: research

Introducing molecular imprinting onto nanozymes: toward selective catalytic analysis
Anal Bioanal Chem. 2024 Feb 3. doi: 10.1007/s00216-024-05183-2. Online ahead of print.ABSTRACTThe discovery of enzyme-like catalytic characteristics in nanomaterials triggers the generation of nanozymes and their multifarious applications. As a class of artificial mimetic enzymes, nanozymes are widely recognized to have better stability and lower cost than natural bio-enzymes, but the lack of catalytic specificity hinders their wider use. To solve the problem, several potential strategies are explored, among which molecular imprinting attracts much attention because of its powerful capacity for creating specific binding ca...
Source: Analytical and Bioanalytical Chemistry - February 3, 2024 Category: Chemistry Authors: Zhijian Bu Lian Huang Shu Li Qingzhen Tian Zheng Tang Qiaoqiao Diao Xinyu Chen Jinjin Liu Xiangheng Niu Source Type: research

Development of target-based cell membrane affinity ultrafiltration technology for a simplified approach to discovering potential bioactive compounds in natural products
Anal Bioanal Chem. 2024 Feb 2. doi: 10.1007/s00216-024-05166-3. Online ahead of print.ABSTRACTTarget-based drug discovery technology based on cell membrane targets has gained significant traction and has been steadily advancing. However, current methods still face certain limitations that need to be addressed. One of the challenges is the laborious preparation process of screening materials, which can be time-consuming and resource-intensive. Additionally, there is a potential issue of non-specific adsorption caused by carrier materials, which can result in false-positive results and compromise the accuracy of the screenin...
Source: Analytical and Bioanalytical Chemistry - February 2, 2024 Category: Chemistry Authors: Xuan Chen Jian-Liang Zhou Jinhao Yu Ningbo Chen Wenda Chen Huaqiu Lu Gui-Zhong Xin Yuanyuan Lin Source Type: research

Establishment of a targeted analysis method for gangliosides in mouse tissues by HILIC-ESI-MS/MS
Anal Bioanal Chem. 2024 Feb 2. doi: 10.1007/s00216-024-05169-0. Online ahead of print.ABSTRACTGangliosides play an imperative role in cell signaling, neuronal recovery, apoptosis, and other physiological processes. For example, GM3 can regulate hypothalamic leptin resistance and control energy homeostasis, GD3 can mediate cell proliferation and differentiation and induce apoptosis, and GQ1b can stimulate neurogenesis. Therefore, the present study sought to establish and optimize the targeted analysis method for ganglioside subclasses and their molecular species using hydrophilic interaction liquid chromatography-triple qua...
Source: Analytical and Bioanalytical Chemistry - February 2, 2024 Category: Chemistry Authors: Shuo Yang Yingxu Ma Yu Song Xiaoxu Wang Peixu Cong Nan Meng Jie Xu Changhu Xue Source Type: research

Comparative analysis of aptamers binding to SARS-CoV-2 N protein using capillary electrophoresis and bio-layer interferometry
This study aims to characterize the binding of aptamers to SARS-CoV-2 nucleocapsid (N) protein using capillary electrophoresis (CE) and bio-layer interferometry (BLI). These two analytical methods differ by how the aptamer binds to its target protein once the aptamer, as a capture ligand, is partitioned in solution (CE) or immobilized on the biosensor (BLI). With CE, the KD values of the N-binding aptamers (tNSP1, tNSP2, and tNSP3) were determined to be 18 ± 4 nM, 45 ± 11 nM, and 32 ± 7 nM, respectively, while the KD measurements by BLI yielded 4.8 ± 0.6, 4.5 ± 0.5, and 2.9 ± 0.3 nM, respectively. CE results showed a...
Source: Analytical and Bioanalytical Chemistry - February 2, 2024 Category: Chemistry Authors: Gurcharan K Uppal Suttinee Poolsup Emil Zaripov Yuxuan Gu Maxim V Berezovski Source Type: research

Correction to: Physical origin of the peak tailing of monoclonal antibodies in size-exclusion chromatography using bio-compatible systems and columns
Anal Bioanal Chem. 2024 Feb;416(5):1309. doi: 10.1007/s00216-024-05167-2.NO ABSTRACTPMID:38305862 | DOI:10.1007/s00216-024-05167-2 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - February 2, 2024 Category: Chemistry Authors: Fabrice Gritti Sornanathan Meyyappan Source Type: research

Non-target screening in water analysis: recent trends of data evaluation, quality assurance, and their future perspectives
Anal Bioanal Chem. 2024 Feb 1. doi: 10.1007/s00216-024-05153-8. Online ahead of print.ABSTRACTThis trend article provides an overview of recent advancements in Non-Target Screening (NTS) for water quality assessment, focusing on new methods in data evaluation, qualification, quantification, and quality assurance (QA/QC). It highlights the evolution in NTS data processing, where open-source platforms address challenges in result comparability and data complexity. Advanced chemometrics and machine learning (ML) are pivotal for trend identification and correlation analysis, with a growing emphasis on automated workflows and r...
Source: Analytical and Bioanalytical Chemistry - February 1, 2024 Category: Chemistry Authors: Maryam Vosough Torsten C Schmidt Gerrit Renner Source Type: research

Screen-printed wearable skin surface pH sensor for real-time monitoring of the buffering capacity of human skin
This study demonstrated for the first time that skin surface pH can be monitored in real-time, using a screen-printed wearable pH sensor, to evaluate the buffering capacity of the human skin. The screen-printed pH sensor was composed of a polyaniline-based pH-sensitive electrode and a nitrocellulose membrane-based liquid junction type of Ag/AgCl reference electrode. This sensor showed a reliable and reversible potentiometric response to pH with long-term potential stability. Intermittent monitoring of the buffering capacity of skin surface pH demonstrated the reliability of the proposed wearable pH sensor, which was compar...
Source: Analytical and Bioanalytical Chemistry - January 31, 2024 Category: Chemistry Authors: Kentaro Chiba Yutaro Harada Hirotaka Matsumoto Hiroyuki Matsui Naoya Ito Tomohito Sekine Kuniaki Nagamine Source Type: research

CE-MS/MS and CE-timsTOF to separate and characterize intramolecular disulfide bridges of monoclonal antibody subunits and their application for the assessment of subunit reduction protocols
Anal Bioanal Chem. 2024 Feb 1. doi: 10.1007/s00216-024-05161-8. Online ahead of print.ABSTRACTCharacterization at the subunit level enables detailed mass spectrometric characterization of posttranslational modifications (PTMs) of monoclonal antibodies (mAbs). The implemented reduction often leaves the intramolecular disulfide bridges intact. Here, we present a capillary electrophoretic (CE) method based on a neutral-coated capillary for the separation of immunoglobulin G-degrading enzyme of Streptococcus pyogenes (IdeS) digested and reduced mAb subunits followed by mass spectrometry (MS), MS/MS identification, and trapped ...
Source: Analytical and Bioanalytical Chemistry - January 31, 2024 Category: Chemistry Authors: Jasmin Schairer Jennifer R ömer Dietmar Lang Christian Neus üß Source Type: research

Electrochemical and biosensor techniques to monitor neurotransmitter changes with depression
Anal Bioanal Chem. 2024 Jan 30. doi: 10.1007/s00216-024-05136-9. Online ahead of print.ABSTRACTDepression is a common mental illness. However, its current treatments, like selective serotonin reuptake inhibitors (SSRIs) and micro-dosing ketamine, are extremely variable between patients and not well understood. Three neurotransmitters: serotonin, histamine, and glutamate, have been proposed to be key mediators of depression. This review focuses on analytical methods to quantify these neurotransmitters to better understand neurological mechanisms of depression and how they are altered during treatment. To quantitatively meas...
Source: Analytical and Bioanalytical Chemistry - January 30, 2024 Category: Chemistry Authors: Kelly E Dunham B Jill Venton Source Type: research

Establishing performance metrics for quantitative non-targeted analysis: a demonstration using per- and polyfluoroalkyl substances
Anal Bioanal Chem. 2024 Feb;416(5):1249-1267. doi: 10.1007/s00216-023-05117-4. Epub 2024 Jan 30.ABSTRACTNon-targeted analysis (NTA) is an increasingly popular technique for characterizing undefined chemical analytes. Generating quantitative NTA (qNTA) concentration estimates requires the use of training data from calibration "surrogates," which can yield diminished predictive performance relative to targeted analysis. To evaluate performance differences between targeted and qNTA approaches, we defined new metrics that convey predictive accuracy, uncertainty (using 95% inverse confidence intervals), and reliability (the ext...
Source: Analytical and Bioanalytical Chemistry - January 30, 2024 Category: Chemistry Authors: Shirley Pu James P McCord Jacqueline Bangma Jon R Sobus Source Type: research

Paper-based uric acid assay in whole blood samples by Zn 2+ protein precipitation and enzyme-free colorimetric detection
Anal Bioanal Chem. 2024 Jan 30. doi: 10.1007/s00216-024-05160-9. Online ahead of print.ABSTRACTUric acid (UA) is an important biomarker, as a high concentration in blood can lead to gout and further renal syndrome. Although several point-of-care testing (POCT) devices have been reported to detect UA, there are some limitations such as the requirement for uricase and the complicated pretreatment of serum/plasma samples, which restricts their use at home or in undeveloped areas. In this work, we developed an approach by applying Zn2+ to precipitate proteins and cells in whole blood to avoid interference with the chromogenic ...
Source: Analytical and Bioanalytical Chemistry - January 30, 2024 Category: Chemistry Authors: Xue Jin Jia Liu Jiaxi Wang Mingxia Gao Xiangmin Zhang Source Type: research

Quantification of [ 99 Tc]TcO 4 - in urine by means of anion-exchange chromatography-aerosol desolvation nebulization-inductively coupled plasma-mass spectrometry
Anal Bioanal Chem. 2024 Jan 30. doi: 10.1007/s00216-024-05149-4. Online ahead of print.ABSTRACTTo sensitively determine 99Tc, a new method for internal quantification of its most common and stable species, [99Tc]Tc[Formula: see text], was developed. Anion-exchange chromatography (IC) was coupled to inductively coupled plasma-mass spectrometry (ICP-MS) and equipped with an aerosol desolvation system to provide enhanced detection power. Due to a lack of commercial Tc standards, an isotope dilution-like approach using a Ru spike and called isobaric dilution analysis (IBDA) was used for internal quantification of 99Tc. This ap...
Source: Analytical and Bioanalytical Chemistry - January 30, 2024 Category: Chemistry Authors: Maximilian Horstmann C Derrick Quarles Steffen Happel Michael Sperling Andreas Faust Kambiz Rahbar David Clases Uwe Karst Source Type: research