Analytical and Bioanalytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A critical evaluation of ultrasensitive single-cell proteomics strategies
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05171-6. Online ahead of print.ABSTRACTSuccess of mass spectrometry characterization of the proteome of single cells allows us to gain a greater understanding than afforded by transcriptomics alone but requires clear understanding of the tradeoffs between analytical throughput and precision. Recent advances in mass spectrometry acquisition techniques, including updated instrumentation and sample preparation, have improved the quality of peptide signals obtained from single cell data. However, much of the proteome remains uncharacterized, and higher throughput techniqu...
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Mary Rachel Nalehua Joseph Zaia Source Type: research

A colorimetric sensing strategy based on chitosan-stabilized platinum nanoparticles for quick detection of α-glucosidase activity and inhibitor screening
In this study, we developed a rapid colorimetric technique using platinum nanoparticles stabilized by chitosan (Ch-PtNPs) to detect α-Glu activity and its inhibitor. The Ch-PtNPs facilitate the conversion of 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of dissolved O2. The catalytic hydrolysis of 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) by α-Glu produces ascorbic acid (AA), which reduces oxTMB to TMB, leading to the fading of the blue color. However, the presence of α-Glu inhibitors (AGIs) hinders the generation of AA, allowing Ch-PtNPs to re-oxidize colorless TMB back to blue ox...
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Qin-Qin Yang Shao-Bin He Yi-Lin Zhang Min Li Xiu-Hua You Bo-Wen Xiao Liu Yang Zhi-Qiang Yang Hao-Hua Deng Wei Chen Source Type: research

Novel insights into the dependence of adsorption-desorption kinetics on particle geometry in chiral chromatography
This study has confirmed that, on both particle formats, analyte diffusion on the surface of the particle is negligible, meaning that adsorption is localized, and it has been demonstrated that adsorption-desorption kinetics is strongly dependent on particle geometry and, in particular, on the loading of chiral selector. These findings are fundamental not only to unravel novel aspects of the complex enantiorecognition mechanism but also to optimize the employment of CSPs for ultra-fast and preparative applications.PMID:38358532 | DOI:10.1007/s00216-024-05186-z (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Chiara De Luca Greta Compagnin Chiara Nosengo Giulia Mazzoccanti Francesco Gasparrini Alberto Cavazzini Martina Catani Simona Felletti Source Type: research

Carbamoylase-based impedimetric electronic tongue for rapid detection of paralytic shellfish toxins
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05199-8. Online ahead of print.ABSTRACTPhytotoxins produced by marine microalgae, such as paralytic shellfish toxins (PSTs), can accumulate in bivalve molluscs, representing a human health concern due to the life-threatening symptoms they cause. To avoid the commercialization of contaminated bivalves, monitoring programs were established in the EU. The purpose of this work is the implementation of a PST transforming enzyme-carbamoylase-in an impedimetric test for rapid simultaneous detection of several carbamate and N-sulfocarbamoyl PSTs. Carbamoylase hydrolyses carba...
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Mariana Raposo Silvia Soreto Catarina Moreirinha Maria Teresa S R Gomes Sara T Costa Maria Jo ão Botelho Bruno M G Melo Lu ís Cadillon Costa Alisa Rudnitskaya Source Type: research

Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05197-w. Online ahead of print.ABSTRACTHand, foot, and mouth disease (HFMD) caused by various enteroviruses is a major public health concern globally. Human enterovirus 71(EVA71), coxsackievirus A16 (CVA16), coxsackievirus A6 (CVA6), and coxsackievirus A10 (CVA10) are four major enteroviruses responsible for HFMD. Rapid, accurate, and specific point-of-care (POC) detection of the four enteroviruses is crucial for the prevention and control of HFMD. Here, we developed two multiplex high-fidelity DNA polymerase loop-mediated isothermal amplification (mHiFi-LAMP) assays ...
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Xiaoling Zhang Yongjuan Zhao Xiaoyi Zhu Weimin Tian Chiyu Zhang Source Type: research

Efficient preparation of high-purity and intact mesenchymal stem cell-derived extracellular vesicles
In this study, we present the adoption of a label-free technique known as "EXODUS" to isolate and purify MSC-EVs from the conditioned medium. Our findings indicate that EXODUS can rapidly isolate EVs from 10 mL of conditioned medium with a 5-fold higher yield compared to conventional approaches, including ultracentrifugation (UC) and polyethylene glycol precipitation (PEG) methods. Additionally, pre-storing the conditioned medium at 4°C for 1 week resulted in a ~2-fold higher yield of MSC-EVs compared to the freshly prepared medium. However, storing the purified EV particles at 4°C for 1 month led to a 2-fold reduction i...
Source: Analytical and Bioanalytical Chemistry - February 14, 2024 Category: Chemistry Authors: Fangfang Ni Qingfu Zhu Hengrui Li Fei Liu Hao Chen Source Type: research

Rapid online analysis of n-alkanes in gaseous streams via APCI mass spectrometry
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05182-3. Online ahead of print.ABSTRACTOnline monitoring of dynamic chemical processes involving a wide volatility range of hydrocarbon species is challenging due to long chromatographic measurement times. Mass spectrometry (MS) overcomes chromatographic delays. However, the analysis of n-alkane mixtures by MS is difficult because many fragment ions are formed, which leads to overlapping signals of the homologous series. Atmospheric pressure chemical ionization (APCI) is suitable for the analysis of saturated hydrocarbons and is the subject of current research. Still,...
Source: Analytical and Bioanalytical Chemistry - February 14, 2024 Category: Chemistry Authors: Jonas Wentrup Ingmar B ösing Thomas D ülcks Jorg Th öming Source Type: research

A label-free aptasensing method for detecting SARS-CoV-2 virus antigen by using dumbbell probe-mediated circle-to-circle amplification
Anal Bioanal Chem. 2024 Feb 13. doi: 10.1007/s00216-024-05195-y. Online ahead of print.ABSTRACTControlling the spread of pathogen requires an efficient and accurate diagnosis. Compared with nucleic acid and antibody detection, antigen assays are more convenient to meet clinical diagnostic needs. However, antigen detection is often difficult to achieve high sensitivity in a limited time. In this work, a novel aptasensing method was designed for the purpose of SARS-CoV-2 antigen detection, using a dumbbell padlock probe-mediated circle-to-circle amplification (C2CA) approach. A sandwich complex of antibody-antigen-aptamer is...
Source: Analytical and Bioanalytical Chemistry - February 13, 2024 Category: Chemistry Authors: Zecheng Wang Si He Chenchen Zhang Danke Xu Source Type: research

LIBS as a novel tool for the determination of the imidization degree of polyimides
Anal Bioanal Chem. 2024 Feb 13. doi: 10.1007/s00216-024-05163-6. Online ahead of print.ABSTRACTDue to their outstanding chemical and physical properties, polyimides are widely used in industrial applications. The degree of imidization of polyimides significantly influences their properties, making it an important factor in tailoring the material for specific applications. Imidization refers to the process of converting a precursor poly(amic acid) by removing water, and it is essential to analyze this process in detail to tune the final structure and properties of the material. Conventional techniques for this task include ...
Source: Analytical and Bioanalytical Chemistry - February 13, 2024 Category: Chemistry Authors: Birgit Achleitner Laurie Girault Silvia Larisegger Michael Nelhiebel Patrick Knaack Andreas Limbeck Source Type: research

A green-footprint approach for parallel multiclass analysis of contaminants in roasted coffee via LC-HRMS
Anal Bioanal Chem. 2024 Feb 13. doi: 10.1007/s00216-024-05157-4. Online ahead of print.ABSTRACTThe development and validation of a simple, comprehensive, and environment-friendly procedure to determine pesticide residues, naturally occurring and processing contaminants in roasted coffee is presented. A solid-liquid extraction of pesticides and mycotoxins with ethyl acetate and the concurrent partition of acrylamide to an aqueous phase follows a parallel analytical strategy that requires a single analytical portion to determine contaminants that are typically analyzed by dedicated single residue methods. The partition rules...
Source: Analytical and Bioanalytical Chemistry - February 13, 2024 Category: Chemistry Authors: Julio C ésar España Amórtegui Susanne Ekroth Heidi Pekar Jairo Arturo Guerrero Dallos Source Type: research