Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research

Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi
CONCLUSIONS: Optimizing fungal colonization and ligninolytic enzyme production, such as manganese peroxidase and laccase, significantly enhances the efficiency of fungal pretreatment. Integrating anaerobic fungi through bioaugmentation during mainstream processing demonstrably increases methane production. This study opens promising avenues for further research and development of these microorganisms for bioenergy production.PMID:38145708 | DOI:10.1016/j.anaerobe.2023.102815 (Source: Anaerobe)
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: R Tamilselvan A Immanuel Selwynraj Source Type: research

In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials
Anaerobe. 2023 Dec 23;85:102816. doi: 10.1016/j.anaerobe.2023.102816. Online ahead of print.ABSTRACTThe aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive anaerobic cocci. Only two (0.8 %) isolates of Bacteroides spp. showed a MIC ≥4 μg/mL. With some exceptions, the pr...
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: Fernando Cobo Ana Franco-Acosta Lina Mart ín-Hita Javier Rodr íguez-Granger Antonio Sampedro-Mart ínez Juan Antonio Reguera-M árquez Jos é María Navarro-Marí Source Type: research

Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi
CONCLUSIONS: Optimizing fungal colonization and ligninolytic enzyme production, such as manganese peroxidase and laccase, significantly enhances the efficiency of fungal pretreatment. Integrating anaerobic fungi through bioaugmentation during mainstream processing demonstrably increases methane production. This study opens promising avenues for further research and development of these microorganisms for bioenergy production.PMID:38145708 | DOI:10.1016/j.anaerobe.2023.102815 (Source: Anaerobe)
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: R Tamilselvan A Immanuel Selwynraj Source Type: research

In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials
Anaerobe. 2023 Dec 23;85:102816. doi: 10.1016/j.anaerobe.2023.102816. Online ahead of print.ABSTRACTThe aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive anaerobic cocci. Only two (0.8 %) isolates of Bacteroides spp. showed a MIC ≥4 μg/mL. With some exceptions, the pr...
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: Fernando Cobo Ana Franco-Acosta Lina Mart ín-Hita Javier Rodr íguez-Granger Antonio Sampedro-Mart ínez Juan Antonio Reguera-M árquez Jos é María Navarro-Marí Source Type: research

Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi
CONCLUSIONS: Optimizing fungal colonization and ligninolytic enzyme production, such as manganese peroxidase and laccase, significantly enhances the efficiency of fungal pretreatment. Integrating anaerobic fungi through bioaugmentation during mainstream processing demonstrably increases methane production. This study opens promising avenues for further research and development of these microorganisms for bioenergy production.PMID:38145708 | DOI:10.1016/j.anaerobe.2023.102815 (Source: Anaerobe)
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: R Tamilselvan A Immanuel Selwynraj Source Type: research

In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials
Anaerobe. 2023 Dec 23;85:102816. doi: 10.1016/j.anaerobe.2023.102816. Online ahead of print.ABSTRACTThe aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive anaerobic cocci. Only two (0.8 %) isolates of Bacteroides spp. showed a MIC ≥4 μg/mL. With some exceptions, the pr...
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: Fernando Cobo Ana Franco-Acosta Lina Mart ín-Hita Javier Rodr íguez-Granger Antonio Sampedro-Mart ínez Juan Antonio Reguera-M árquez Jos é María Navarro-Marí Source Type: research

Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi
CONCLUSIONS: Optimizing fungal colonization and ligninolytic enzyme production, such as manganese peroxidase and laccase, significantly enhances the efficiency of fungal pretreatment. Integrating anaerobic fungi through bioaugmentation during mainstream processing demonstrably increases methane production. This study opens promising avenues for further research and development of these microorganisms for bioenergy production.PMID:38145708 | DOI:10.1016/j.anaerobe.2023.102815 (Source: Anaerobe)
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: R Tamilselvan A Immanuel Selwynraj Source Type: research

In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials
Anaerobe. 2023 Dec 23;85:102816. doi: 10.1016/j.anaerobe.2023.102816. Online ahead of print.ABSTRACTThe aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive anaerobic cocci. Only two (0.8 %) isolates of Bacteroides spp. showed a MIC ≥4 μg/mL. With some exceptions, the pr...
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: Fernando Cobo Ana Franco-Acosta Lina Mart ín-Hita Javier Rodr íguez-Granger Antonio Sampedro-Mart ínez Juan Antonio Reguera-M árquez Jos é María Navarro-Marí Source Type: research

Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi
CONCLUSIONS: Optimizing fungal colonization and ligninolytic enzyme production, such as manganese peroxidase and laccase, significantly enhances the efficiency of fungal pretreatment. Integrating anaerobic fungi through bioaugmentation during mainstream processing demonstrably increases methane production. This study opens promising avenues for further research and development of these microorganisms for bioenergy production.PMID:38145708 | DOI:10.1016/j.anaerobe.2023.102815 (Source: Anaerobe)
Source: Anaerobe - December 25, 2023 Category: Microbiology Authors: R Tamilselvan A Immanuel Selwynraj Source Type: research