Density of high endothelial venules and PDL-1 expression: relationship with tumor-infiltrating lymphocytes in primary cutaneous melanomas
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230441. doi: 10.1590/0001-3765202420230441. eCollection 2024.ABSTRACTStudies have highlighted melanoma immunogenicity, and the prognostic importance of tumor infiltrating lymphocytes (TILs) and mechanisms of tumor immune evasion, such as hyperexpression of programmed cell death ligand 1 (PDL-1). High endothelial venules (HEV) are specialized blood vessels that can facilitate the lymphocytes migration to the tumor. Here we evaluate the association of HEV density and PDL-1 expression in primary cutaneous melanomas with the presence and degree of TILs and with other clinicopathological...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Josiane B Stolfo Adriana C DA Motta Source Type: research
In vitro cultivation of Vismia japurensis: Isolation of the new anthrone 1,8,10-trihydroxy-3,10-dimethyl-9(10H)-anthracenone
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230456. doi: 10.1590/0001-3765202420230456. eCollection 2024.ABSTRACTVismia japurensis Reichardt is a plant of ecological and chemical importance from which a variety of bioactive substances have been isolated. The current study aimed to establish in vitro cultures of this species as a source of secondary metabolites. Appropriate decontamination treatments and germination tests were performed and, after in vitro culture establishment, the propagated plants were multiplied in a sterile environment to increase the biomass of available experimental material. Seeds showed low contaminat...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: La ísley M Lima Weison L Silva Julio Cezar DE Souza Cecilia V Nunez Source Type: research
High infestation and phylogenetic position of Epistylis sp. (Ciliophora, Peritrichia) on Aegla serrana Buckup & amp; Rossi (Crustacea, Anomura) from southern Brazil
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230739. doi: 10.1590/0001-3765202420230739. eCollection 2024.ABSTRACTHigh infestations of epibiont ciliates on vertebrates or invertebrates are normally related to aquaculture tanks or similar environments, and the importance of this relationship in natural habitats is often disregarded. Here, we describe the first record of high infestation of ciliates on Aegla serrana in South America and conduct a brief morphological and phylogenetic characterization of these ciliates. Our findings confirm that cases of high infestation of ciliates on metazoans can indeed occur in natural environ...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Pedro M DE Souza Roberto J únio P Dias Arthur Loures Mariana F Rossi Jos é Felipe R Amato Marta D'Agosto Source Type: research
Development of benzaldehyde-pyrazoline hybrids for functionalization of polymers with fluorescent pendant moieties
An Acad Bras Cienc. 2024 Mar 18;96(1):e20220875. doi: 10.1590/0001-3765202420220875. eCollection 2024.ABSTRACTCompounds with a pyrazoline scaffold are useful as sensors for fluorescence detection of different types of analytes. Recovery of a pyrazoline-based sensor with a view to use it recurrently would be more facile when the sensing molecule is attached to a solid support. A reaction sequence has been designed to synthesize two benzaldehyde-pyrazoline hybrids as examples of a hitherto unknown type of compounds to be employed for the potential derivatization of polymers containing primary amino groups through azomethine ...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Gheorghe Roman Mihaela Balan-Porc ăraşu Liviu S ăcărescu Source Type: research
Distribution of gamma-ray elements in Cerro do Jarau impact structure and a proposal of geothermal modeling
This study collected ground gamma-ray data over the structure and produced K, eTh and eU concentrations, eTh/K ratio and F-factor maps to characterize it in terms of its radiometric signatures and their respective spatial distribution. A general decrease in the concentration of the three elements was observed from the outer-rim towards the center of the crater. The central area is defined by very low radiometric values, with relatively high K values, thus indicating the occurrence of K-bearing rocks. Numerical simulations using the HYDROTHERM 3 code showed the fluid circulation pattern over the impact structure. Data inter...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Diego D Garcia Emilson P Leite Marcos Alberto R Vasconcelos Evangelos Christou Alvaro P Cr ósta Source Type: research
Influence of Chemical Nature of Citric and Tartaric Acids on Reaction Time of the Crosslinking of Polyvinyl Alcohol Hydrogels
In this study, PVOH hydrogels were synthesized by a solution under stirring and heating techniques with citric (AC) and tartaric acids (AT) crosslinker agents, with different time reactions of 20 min.; 1; 2, and 3 h were investigated. These samples were characterized by the kinetics of water uptake, gel fraction, thermal analysis, and physical-chemical analysis, and their structure was elucidated. The results obtained have shown chemical modification by organic acids and improved the properties to good thermal stability and swelling to AT hydrogels up to 900% water uptake. In the gel fraction, the samples' esterification w...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Dione P DE Castro Ruth Marlene C Santana Source Type: research
Sequential extraction of anthocyanins and pectin from jabuticaba (Plinia cauliflora) peel: Peel pretreatment effect and ultrasound-assisted extraction
The objective was to evaluate the effect of pretreatment and fresh use of jabuticaba peels in the extraction of total phenolic compound (TPC) and total anthocyanin (TA) contents with and without ultrasound assistance and in the sequential extraction of pectin from the residue. In the TPC and TA extraction, a 3x2 factorial design was used. For conventional anthocyanin extraction (CAE), occurred in an incubator under agitation. For ultrasound-assisted anthocyanin extraction (UAE) was utilized an ultrasonic homogenizer with probe (20 kHz, 160 W). The extracts were quantified (TPC, TA, antioxidant activity and color). The resi...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Tainah M Bueno Fabiana Queiroz Jhenifer Cristina C Dos Santos Maria Luiza B Furtado Maria Cec ília E V Schiassi Soraia V Borges Jayne A Figueiredo Source Type: research
Density of high endothelial venules and PDL-1 expression: relationship with tumor-infiltrating lymphocytes in primary cutaneous melanomas
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230441. doi: 10.1590/0001-3765202420230441. eCollection 2024.ABSTRACTStudies have highlighted melanoma immunogenicity, and the prognostic importance of tumor infiltrating lymphocytes (TILs) and mechanisms of tumor immune evasion, such as hyperexpression of programmed cell death ligand 1 (PDL-1). High endothelial venules (HEV) are specialized blood vessels that can facilitate the lymphocytes migration to the tumor. Here we evaluate the association of HEV density and PDL-1 expression in primary cutaneous melanomas with the presence and degree of TILs and with other clinicopathological...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Josiane B Stolfo Adriana C DA Motta Source Type: research
In vitro cultivation of Vismia japurensis: Isolation of the new anthrone 1,8,10-trihydroxy-3,10-dimethyl-9(10H)-anthracenone
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230456. doi: 10.1590/0001-3765202420230456. eCollection 2024.ABSTRACTVismia japurensis Reichardt is a plant of ecological and chemical importance from which a variety of bioactive substances have been isolated. The current study aimed to establish in vitro cultures of this species as a source of secondary metabolites. Appropriate decontamination treatments and germination tests were performed and, after in vitro culture establishment, the propagated plants were multiplied in a sterile environment to increase the biomass of available experimental material. Seeds showed low contaminat...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: La ísley M Lima Weison L Silva Julio Cezar DE Souza Cecilia V Nunez Source Type: research
High infestation and phylogenetic position of Epistylis sp. (Ciliophora, Peritrichia) on Aegla serrana Buckup & amp; Rossi (Crustacea, Anomura) from southern Brazil
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230739. doi: 10.1590/0001-3765202420230739. eCollection 2024.ABSTRACTHigh infestations of epibiont ciliates on vertebrates or invertebrates are normally related to aquaculture tanks or similar environments, and the importance of this relationship in natural habitats is often disregarded. Here, we describe the first record of high infestation of ciliates on Aegla serrana in South America and conduct a brief morphological and phylogenetic characterization of these ciliates. Our findings confirm that cases of high infestation of ciliates on metazoans can indeed occur in natural environ...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Pedro M DE Souza Roberto J únio P Dias Arthur Loures Mariana F Rossi Jos é Felipe R Amato Marta D'Agosto Source Type: research
Development of benzaldehyde-pyrazoline hybrids for functionalization of polymers with fluorescent pendant moieties
An Acad Bras Cienc. 2024 Mar 18;96(1):e20220875. doi: 10.1590/0001-3765202420220875. eCollection 2024.ABSTRACTCompounds with a pyrazoline scaffold are useful as sensors for fluorescence detection of different types of analytes. Recovery of a pyrazoline-based sensor with a view to use it recurrently would be more facile when the sensing molecule is attached to a solid support. A reaction sequence has been designed to synthesize two benzaldehyde-pyrazoline hybrids as examples of a hitherto unknown type of compounds to be employed for the potential derivatization of polymers containing primary amino groups through azomethine ...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Gheorghe Roman Mihaela Balan-Porc ăraşu Liviu S ăcărescu Source Type: research
Distribution of gamma-ray elements in Cerro do Jarau impact structure and a proposal of geothermal modeling
This study collected ground gamma-ray data over the structure and produced K, eTh and eU concentrations, eTh/K ratio and F-factor maps to characterize it in terms of its radiometric signatures and their respective spatial distribution. A general decrease in the concentration of the three elements was observed from the outer-rim towards the center of the crater. The central area is defined by very low radiometric values, with relatively high K values, thus indicating the occurrence of K-bearing rocks. Numerical simulations using the HYDROTHERM 3 code showed the fluid circulation pattern over the impact structure. Data inter...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Diego D Garcia Emilson P Leite Marcos Alberto R Vasconcelos Evangelos Christou Alvaro P Cr ósta Source Type: research
Influence of Chemical Nature of Citric and Tartaric Acids on Reaction Time of the Crosslinking of Polyvinyl Alcohol Hydrogels
In this study, PVOH hydrogels were synthesized by a solution under stirring and heating techniques with citric (AC) and tartaric acids (AT) crosslinker agents, with different time reactions of 20 min.; 1; 2, and 3 h were investigated. These samples were characterized by the kinetics of water uptake, gel fraction, thermal analysis, and physical-chemical analysis, and their structure was elucidated. The results obtained have shown chemical modification by organic acids and improved the properties to good thermal stability and swelling to AT hydrogels up to 900% water uptake. In the gel fraction, the samples' esterification w...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Dione P DE Castro Ruth Marlene C Santana Source Type: research
Sequential extraction of anthocyanins and pectin from jabuticaba (Plinia cauliflora) peel: Peel pretreatment effect and ultrasound-assisted extraction
The objective was to evaluate the effect of pretreatment and fresh use of jabuticaba peels in the extraction of total phenolic compound (TPC) and total anthocyanin (TA) contents with and without ultrasound assistance and in the sequential extraction of pectin from the residue. In the TPC and TA extraction, a 3x2 factorial design was used. For conventional anthocyanin extraction (CAE), occurred in an incubator under agitation. For ultrasound-assisted anthocyanin extraction (UAE) was utilized an ultrasonic homogenizer with probe (20 kHz, 160 W). The extracts were quantified (TPC, TA, antioxidant activity and color). The resi...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Tainah M Bueno Fabiana Queiroz Jhenifer Cristina C Dos Santos Maria Luiza B Furtado Maria Cec ília E V Schiassi Soraia V Borges Jayne A Figueiredo Source Type: research
Density of high endothelial venules and PDL-1 expression: relationship with tumor-infiltrating lymphocytes in primary cutaneous melanomas
An Acad Bras Cienc. 2024 Mar 18;96(1):e20230441. doi: 10.1590/0001-3765202420230441. eCollection 2024.ABSTRACTStudies have highlighted melanoma immunogenicity, and the prognostic importance of tumor infiltrating lymphocytes (TILs) and mechanisms of tumor immune evasion, such as hyperexpression of programmed cell death ligand 1 (PDL-1). High endothelial venules (HEV) are specialized blood vessels that can facilitate the lymphocytes migration to the tumor. Here we evaluate the association of HEV density and PDL-1 expression in primary cutaneous melanomas with the presence and degree of TILs and with other clinicopathological...
Source: An Acad Bras Cienc - March 21, 2024 Category: Science Authors: Josiane B Stolfo Adriana C DA Motta Source Type: research
