TRPV1 channels facilitate axonal degeneration of corneal sensory nerves in dry eye
Corneal nerve impairment contributes significantly to dry eye disease (DED) symptoms and is thought to be secondary to corneal epithelial damage. Transient receptor potential vanilloid-1 (TRPV1) channels abound in corneal nerve fibers and respond to inflammation-derived ligands, which increase in DED. TRPV1 overactivation promotes axonal degeneration in vitro but whether it participates in DED-associated corneal nerve dysfunction is unknown. To explore this, DED was surgically induced in wild-type and TRPV1-knockout (Trpv1KO) mice, which developed comparable corneal epithelial damage and reduced tear secretion. (Source: Am...
Source: American Journal of Pathology - February 5, 2024 Category: Pathology Authors: Manuela Pizzano, Alexia Vereertbrugghen, Agostina Cernutto, Florencia Sabbione, Irene A. Keitelman, Carolina M. Shiromizu, Douglas Vera Aguilar, Federico Fuentes, Mirta N. Giordano, Anal ía S. Trevani, Jeremías G. Galletti Tags: Regular Article Source Type: research
Basic Transcription Factor 3 Like 4 Enhances Malignant Phenotypes through Modulating Tumor Cell Function and Immune Microenvironment in Glioma
Recent investigations into the tumor microenvironment have provided insights into the limited response of glioma progression to immunotherapy. However, the specific involvement of basic transcription factor 3 like 4 (BTF3L4) in glioma progression and its correlation with immune cell infiltration remain areas of uncertainty that require further exploration. In the current study, BTF3L4 expression was delineated by using gene expression profiling/interactive analysis and multiplex-immunohistologic staining of tissue microarrays. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 4, 2024 Category: Pathology Authors: Bing Lu, Tianyi Lu, Jiawen Shi, Pingping Sun, Xiaojng Zhang, Lei Yang, Ziheng Wang, Jianfei Huang Tags: Regular article Source Type: research
Model-Agnostic Binary Patch Grouping for Bone Marrow Whole Slide Image Representation
Histopathology is the reference standard for pathology diagnosis, and has evolved with the digitization of glass slides [ie, whole slide images (WSIs)]. Trained histopathologists can help diagnose disease by examining WSIs visually, but this process is time consuming and prone to variability. To address these issues, artificial intelligence models are being developed to generate slide-level representations of WSIs, summarizing the entire slide as a single vector. This enables various computational pathology applications, including interslide search, multimodal training, and slide-level classification. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 4, 2024 Category: Pathology Authors: Youqing Mu, Hamid R. Tizhoosh, Taher Dehkharghanian, Saghir Alfasly, Clinton J.V. Campbell Tags: Regular article Source Type: research
Periostin in Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression by Enhancing Cancer and Stromal Cell Migration
Cancer-associated fibroblasts (CAFs) in the tumor microenvironment are involved in the progression of various cancers, including esophageal squamous cell carcinoma (ESCC). CAF-like cells were generated through direct co-culture of human bone marrow-derived mesenchymal stem cells (MSCs), one of CAF origins, with ESCC cells, and periostin was found to be highly expressed in CAF-like cells. After direct co-culture, ESCC cells showed increased malignant phenotypes such as survival, growth, and migration, as well as increased phosphorylation of Akt and Erk. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 4, 2024 Category: Pathology Authors: Shoji Miyako, Yu-ichiro Koma, Takashi Nakanishi, Shuichi Tsukamoto, Keitaro Yamanaka, Nobuaki Ishihara, Yuki Azumi, Satoshi Urakami, Masaki Shimizu, Takayuki Kodama, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki Tags: Regular Article Source Type: research
BTF3L4 enhances malignant phenotypes through modulating tumor cell function and immune microenvironment in glioma
Recent investigations into the tumor microenvironment have provided insights into the limited response of glioma progression to immunotherapy. However, the specific involvement of Basic Transcription Factor 3 Like 4 (BTF3L4) in glioma progression and its correlation with immune cell infiltration remained areas of uncertainty that require further exploration. In the present study, BTF3L4 expression was delineated using gene-expression-profiling-interactive-analysis and multiplex-immunohistological staining of tissue microarrays. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 4, 2024 Category: Pathology Authors: Bing Lu, Tianyi Lu, Jiawen Shi, Pingping Sun, Xiaojng Zhang, Lei Yang, Ziheng Wang, Jianfei Huang Tags: Regular Article Source Type: research
Model-Agnostic Binary Patch Grouping for Bone Marrow WSI Representation
Histopathology is the reference standard for pathology diagnosis, and has evolved with the digitization of glass slides, i.e. whole slide images (WSIs). Trained histopathologists can help to diagnose disease by examining WSIs visually, but this process is time-consuming and prone to variability. To address these issues, AI models are being developed to create slide-level representations of WSIs, summarizing the entire slide as a single vector. This enables various computational pathology applications, including inter-slide search, multi-modal training, and slide-level classification. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 4, 2024 Category: Pathology Authors: Youqing Mu, Hamid R. Tizhoosh, Taher Dehkharghanian, Saghir Alfasly, Clinton JV. Campbell Tags: Regular Article Source Type: research
IL-6 Up-Regulates Expression of LIM-Domain Only Protein 4 in Psoriatic Keratinocytes through Activation of the MEK/ERK/NF- κB Pathway
Psoriasis is a chronic inflammatory skin disease characterized by the activation of keratinocytes and the infiltration of immune cells. Overexpression of the transcription factor LIM-domain only protein 4 (LMO4) promoted by IL-23 has critical roles in regulating the proliferation and differentiation of psoriatic keratinocytes. IL-6, an autocrine cytokine in psoriatic epidermis, is a key mediator of IL-23/T helper 17 –driven cutaneous inflammation. However, little is known about how IL-6 regulates the up-regulation of LMO4 expression in psoriatic lesions. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 3, 2024 Category: Pathology Authors: Zhenzhen Tu, Wei Wei, Fanjun Zeng, Wenwen Wang, Yuyan Zhang, Yintao Zhang, Fusheng Zhou, Chunlin Cai, Siping Zhang, Haisheng Zhou Tags: Regular article Source Type: research
Histologic Evidence of Epithelial –Mesenchymal Transition and Autophagy in Human Fetal Membranes
Preterm, prelabor rupture of the human fetal membranes (pPROM) is involved in 40% of spontaneous preterm births. Cellular-level disturbances and inflammation are effectors of membrane degradation, weakening, and rupture. Maternal risk factors induce oxidative stress (OS), senescence, and senescence-associated inflammation of the fetal membranes as reported mechanisms related to pPROM. Inflammation can also arise in fetal membrane cells (amnion/chorion) due to OS-induced autophagy and epithelial –mesenchymal transition (EMT). (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 3, 2024 Category: Pathology Authors: Mary E. Severino, Lauren S. Richardson, Marian Kacerovsky, Ramkumar Menon Tags: Regular article Source Type: research
Histologic Evidence of Epithelial-Mesenchymal Transition and Autophagy in Human Fetal Membranes
Preterm prelabor rupture of the human fetal membranes (pPROM) is associated with 40% of spontaneous preterm births. Cellular level disturbances and inflammation are effectors of membrane degradation, weakening, and rupture. Maternal risk factors induce oxidative stress (OS), senescence, and senescence-associated inflammation of the fetal membranes as reported mechanisms related to pPROM. Inflammation can also arise in fetal membrane cells (amnion/chorion) due to OS-induced autophagy and epithelial-mesenchymal transition (EMT). (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 3, 2024 Category: Pathology Authors: Mary Elise Severino, Lauren S. Richardson, Marian Kacerovsky, Ramkumar Menon Tags: Regular Article Source Type: research
IL-6 upregulates expression of LMO4 in psoriatic keratinocytes through activation of the MEK/ERK/NF- κB pathway
Psoriasis is a chronic inflammatory skin disease characterized by the activation of keratinocytes and the infiltration of immune cells. Overexpression of the transcription factor LIM-domain only protein 4 (LMO4) promoted by interleukin-23 (IL-23) has critical roles in regulating the proliferation and differentiation of psoriatic keratinocytes. IL-6, an autocrine cytokine in psoriatic epidermis, is a key mediator of IL-23/T helper (Th17)-driven cutaneous inflammation. However, little is known about how IL-6 regulates the upregulation of LMO4 expression in psoriatic lesions. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 3, 2024 Category: Pathology Authors: Zhenzhen Tu, Wei Wei, Fanjun Zeng, Wenwen Wang, Yuyan Zhang, Yintao Zhang, Fusheng Zhou, Chunlin Cai, Siping Zhang, Haisheng Zhou Tags: Regular Article Source Type: research
5-hydroxytryptamine 4 receptor agonist attenuates diabetic enteric neuropathy through inhibition of the RIPK3 pathway
This study aimed to explore the beneficial effects of 5-HT4R agonist on enteric neuropathy in a mouse model of diabetes and the mechanisms underlying these effects. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 2, 2024 Category: Pathology Authors: Yingying Cheng, Yueting Kou, Juan Wang, Yue Wang, Weifang Rong, Hongxiu Han, Guohua Zhang Tags: Regular Article Source Type: research
Bicellular localization of tricellular junctional protein angulin-3/ILDR2 allows detection of podocyte injury.
This study focused on a tricellular junction protein angulin-3 and its localization was analyzed in healthy podocytes as well as in developmental stage and in pathological conditions, using a newly-established monoclonal antibody. (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 2, 2024 Category: Pathology Authors: Atsuko Y. Higashi, Akira C. Saito, Tomohito Higashi, Kyoko Furuse, Mikio Furuse, Hideki Chiba, Junichiro J. Kazama Tags: Regular Article Source Type: research
PDGF-D is dispensable for the development and progression of murine Alport syndrome
Alport syndrome is an inherited kidney disease, which can lead to glomerulosclerosis and fibrosis, and end-stage kidney disease in children and adults. Platelet-derived growth factor (PDGF)-D was shown to mediate glomerulosclerosis and interstitial fibrosis in different models of kidney disease, prompting us to investigate its role in a murine model of Alport syndrome.In vitro, PDGF-D induced proliferation and profibrotic activation of conditionally immortalized human parietal epithelial cells (ciPECs). (Source: American Journal of Pathology)
Source: American Journal of Pathology - February 1, 2024 Category: Pathology Authors: Emilia Anouk Margo Firat, Eva Miriam Buhl, Nassim Bouteldja, Bart Smeets, Ulf Eriksson, Peter Boor, Barbara Mara Klinkhammer Tags: Regular Article Source Type: research
Diabetes Accelerates Steatohepatitis in Mice
This study investigated the impact of diabetes on steatohepatitis and established a novel mouse model for diabetic steatohepatitis. Male C57BL/6J mice were fed a 60% high-fat diet (HFD) and injected with carbon tetrachloride (CCl4) and streptozotocin (STZ) to induce diabetes. The HFD+CCl4+STZ group showed more severe liver steatosis, hepatocyte ballooning, and regenerative nodules compared with other groups. (Source: American Journal of Pathology)
Source: American Journal of Pathology - January 31, 2024 Category: Pathology Authors: Tuerdiguli Abuduyimiti, Hisanori Goto, Kumi Kimura, Yu Oshima, Ryota Tanida, Kyoko Kamoshita, Nontaphat Leerach, Halimulati Abuduwaili, Hein Ko Oo, Qifang Li, Cynthia M. Galicia-Medina, Hiroaki Takayama, Kiyo-aki Ishii, Yujiro Nakano, Yumie Takeshita, Tom Tags: Regular article Source Type: research
Neutrophil Infiltration and Function in the Pathogenesis of Inflammatory Airspace Disease
Neutrophils are an important cell type often considered the body's "first responder" in the response to inflammatory insult or damage. They are recruited to the tissue of the lungs during inflammatory airspace diseases and can perform unique and complex functions that range from helpful to harmful. Additionally, neutrophil function is unique in the lungs through heterogeneity of the inflammatory cascade and retention in the vasculature. They are known to "marginate" or remain stagnant in the lungs even during non-disease conditions. (Source: American Journal of Pathology)
Source: American Journal of Pathology - January 31, 2024 Category: Pathology Authors: Maureen E. Haynes, David P. Sullivan, William A. Muller Tags: Mini-Review Source Type: research
