Development of Scaffold-Free Three-Dimensional Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis
Organoids are novel in vitro models to study intercellular cross talk between the different types of cells in the disease pathophysiology. To better understand the underlying mechanisms driving the progression of primary sclerosing cholangitis (PSC), we developed scaffold-free multicellular three-dimensional cholangiocy te organoids (3D-CHOs) using primary liver cells derived from normal patients and patients with PSC. Human liver samples from healthy donors and patients with PSC were used to isolate primary cholangiocytes [epithelial cell adhesion molecule (EpCam)+/ cytokeratin-19+], liver endothelial cells (CD31+ ), an...
Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Wenjun Zhang, Konstantina Kyritsi, Abdulkadir Isidan, Yujin Park, Ping Li, Arthur A. Cross-Najafi, Kevin Lopez, Lindsey Kennedy, Keisaku Sato, Shannon Glaser, Heather Francis, Gianfranco Alpini, Burcin Ekser Tags: Regular article Source Type: research
Dysregulation of Spliceosomes Complex Induces Retinitis Pigmentosa –Like Characteristics in sf3b4-Depleted Zebrafish
The SF3B4 gene encodes a highly conserved protein that plays a critical role in mRNA splicing, and mutations in this gene are known to cause Nager syndrome, a rare craniofacial disorder. Although SF3B4 expression is detected earlier in the optic vesicle than in the limb and somite, the role of SF3B4 in the eye is not well understood. We investigate the function of sf3b4 in the retina by performing transcriptome profiles, immunostaining, and behavioral analysis of sf3b4 −/− mutant zebrafish. Our findings suggest that dysregulation of the spliceosome complex affects not only craniofacial development but also retinogenesi...
Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Zulvikar Syambani Ulhaq, Keigo Okamoto, Yukiko Ogino, William Ka Fai Tse Tags: Regular article Source Type: research
Innate Lymphoid Cells and Interferons Limit Neurologic and Articular Complications of Brucellosis
Brucellosis is a globally significant zoonotic disease. Human brucellosis patients develop recurrent fever and focal complications including arthritis and neurobrucellosis. Here, the role of innate lymphoid cells (ILCs) in the pathogenesis of focal brucellosis caused by Brucella melitensis was investigated. After footpad infection, NK cells, and ILC1 cells both limited joint colonization by Brucella. Mice lacking NK cells, and in particular mice lacking all ILCs also developed marked arthritis after footpad infection. (Source: American Journal of Pathology)
Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Charles R. Moley, Catherine A. Chambers, Alexis S. Dadelahi, B árbara Ponzilacqua-Silva, Mostafa F.N. Abushahba, Carolyn A. Lacey, Craig L. Franklin, Jerod A. Skyberg Tags: Regular Article Source Type: research
Transcription factor Foxo1 mediates TGF- β1-induced apoptosis in hepatocytes
In this study, we investigated the potential relationship between TGF-β1 signaling and Foxo1 in control of apoptosis in hepatocytes. (Source: American Journal of Pathology)
Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Yunmei Chen, Quan Pan, Wang Liao, Weiqi Ai, Sijun Yang, Shaodong Guo Tags: Regular Article Source Type: research
Development of Scaffold-free 3D Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis
Organoids are novel in vitro models to study intercellular crosstalk between the different types of cells in the disease pathophysiology. To better understand the underlying mechanisms driving the progression of primary sclerosing cholangitis (PSC), we developed scaffold-free multi-cellular 3D cholangiocyte organoids (3D-CHO) using ‘primary’ liver cells derived from normal and PSC patients. Human liver samples from healthy donors and PSC patients were used to isolate ‘primary’ cholangiocytes (EPCAM+/CK-19+), liver endothelial cells (LECs, CD31+), and hepatic stellate cells (HSCs, CD31-/CD68-/ Desmin+/Vitamin A+). (...
Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Wenjun Zhang, Konstantina Kyritsi, Abdulkadir Isidan, Yujin Park, Ping Li, Arthur A. Cross-Najafi, Kevin Lopez, Lindsey Kennedy, Keisaku Sato, Shannon Glaser, Heather Francis, Gianfranco Alpini, Burcin Ekser Tags: Regular Article Source Type: research
