TGF β1 Drives Integrin Dependent Pericyte Migration and Microvascular Destabilization In Fibrotic Disease
Am J Pathol. 2024 Mar 26:S0002-9440(24)00118-4. doi: 10.1016/j.ajpath.2024.02.021. Online ahead of print.ABSTRACTInteractions between endothelial cells (EC) and mural pericytes (PC) are critical to maintaining the stability and function of the microvascular wall. Abnormal interactions between these two cell types are a hallmark of progressive fibrotic diseases, such as systemic sclerosis (SSc, scleroderma). However, the role that PCs play in signaling microvascular dysfunction remains underexplored. It is hypothesized that integrin-matrix interactions contribute to PC migration from the vascular wall and conversion into in...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Amanda S Pellowe Michelle J Wu Tae-Yun Kang Tracy D Chung Adrian Ledesma-Mendoza Erica Herzog Andre Levchenko Ian Odell John Varga Anjelica L Gonzalez Source Type: research
Enhanced Optic Nerve Expansion and Altered Ultrastructure of Elastic Fibers Induced by Lysyl Oxidase Inhibition in a Mouse Model of Marfan Syndrome
Am J Pathol. 2024 Mar 26:S0002-9440(24)00115-9. doi: 10.1016/j.ajpath.2024.03.002. Online ahead of print.ABSTRACTTwo major constituents of exfoliation material (XFM), fibrillin- 1 and lysyl oxidase-like 1 (encoded by FBN1 and LOXL1) are implicated in exfoliation glaucoma (XFG), yet their individual contributions to ocular phenotype are minor. To test the hypothesis that a combination of FBN1 mutation and LOXL1 deficiency exacerbates ocular phenotypes, pan-LOX inhibitor, β-aminopropionitrile (BAPN) was used to treat adult wild-type (wt) and Fbn1C1041G/+ mice for 8 weeks and their eyes were examined. Although intraocular pr...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hang-Jing Wu Evan Krystofiak John Kuchtey Rachel W Kuchtey Source Type: research
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury
Am J Pathol. 2024 Mar 26:S0002-9440(24)00116-0. doi: 10.1016/j.ajpath.2024.03.003. Online ahead of print.ABSTRACTAcute lung injury (ALI) is a devastating clinical disease caused by different factors, with high morbidity and mortality. It has been shown that lung injury and inflammation caused by lipopolysaccharide (LPS) can be modulated by NLRP3 inflammasome activation, yet its exact function within the airway epithelium is still unknown. Meanwhile, glucose transporter protein 1 (GLUT1) has been shown to contribute to a number of inflammatory illnesses, including ALI. The present study was aimed to assess GLUT1's function ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Jiehong Li Yijian Li Guanjin Chen Yan Liang Jianpeng Xie Shuiying Zhang Kai Zhong Tong Jiang Haisu Yi Haixiong Tang Xin Chen Source Type: research
Poorly Differentiated Hepatocellular Carcinoma Cells Avoid Apoptosis by Interacting with T Cells via CD40-CD40L Linkage
We examined CD40 levels in HCC patient tissues and various HCC cell lines, studying their interaction with CD4+T cells. RNA sequencing analysis was performed to explore the mechanisms of CD40 induction. Poorly differentiated HCC tumour tissues exhibited high membrane-bound CD40 (mCD40) expression, in contrast to non-tumour areas. Poorly differentiated HCC cell lines showed high expression of mCD40 with low CD40 promoter methylation which were opposite of well-differentiated ones. Solely modulating CD40 expression in HCC cells exerted no direct consequences on cell growth or appearance. Interestingly, HLFs co-cultured with ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hanh Vinh Ngo Thuy Thi Thanh Le Hieu Ngoc Vu Hai Hoang Hiroko Ikenaga Misako Sato-Matsubara Sawako Uchida-Kobayashi Hayato Urushima Khanh Van Nguyen Ha Thi Nguyen Hiroji Shinkawa Shoji Kubo Naoko Ohtani Masaru Enomoto Akihiro Tamori Norifumi Kawada Source Type: research
TGF β1 Drives Integrin Dependent Pericyte Migration and Microvascular Destabilization In Fibrotic Disease
Am J Pathol. 2024 Mar 26:S0002-9440(24)00118-4. doi: 10.1016/j.ajpath.2024.02.021. Online ahead of print.ABSTRACTInteractions between endothelial cells (EC) and mural pericytes (PC) are critical to maintaining the stability and function of the microvascular wall. Abnormal interactions between these two cell types are a hallmark of progressive fibrotic diseases, such as systemic sclerosis (SSc, scleroderma). However, the role that PCs play in signaling microvascular dysfunction remains underexplored. It is hypothesized that integrin-matrix interactions contribute to PC migration from the vascular wall and conversion into in...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Amanda S Pellowe Michelle J Wu Tae-Yun Kang Tracy D Chung Adrian Ledesma-Mendoza Erica Herzog Andre Levchenko Ian Odell John Varga Anjelica L Gonzalez Source Type: research
Enhanced Optic Nerve Expansion and Altered Ultrastructure of Elastic Fibers Induced by Lysyl Oxidase Inhibition in a Mouse Model of Marfan Syndrome
Am J Pathol. 2024 Mar 26:S0002-9440(24)00115-9. doi: 10.1016/j.ajpath.2024.03.002. Online ahead of print.ABSTRACTTwo major constituents of exfoliation material (XFM), fibrillin- 1 and lysyl oxidase-like 1 (encoded by FBN1 and LOXL1) are implicated in exfoliation glaucoma (XFG), yet their individual contributions to ocular phenotype are minor. To test the hypothesis that a combination of FBN1 mutation and LOXL1 deficiency exacerbates ocular phenotypes, pan-LOX inhibitor, β-aminopropionitrile (BAPN) was used to treat adult wild-type (wt) and Fbn1C1041G/+ mice for 8 weeks and their eyes were examined. Although intraocular pr...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hang-Jing Wu Evan Krystofiak John Kuchtey Rachel W Kuchtey Source Type: research
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury
Am J Pathol. 2024 Mar 26:S0002-9440(24)00116-0. doi: 10.1016/j.ajpath.2024.03.003. Online ahead of print.ABSTRACTAcute lung injury (ALI) is a devastating clinical disease caused by different factors, with high morbidity and mortality. It has been shown that lung injury and inflammation caused by lipopolysaccharide (LPS) can be modulated by NLRP3 inflammasome activation, yet its exact function within the airway epithelium is still unknown. Meanwhile, glucose transporter protein 1 (GLUT1) has been shown to contribute to a number of inflammatory illnesses, including ALI. The present study was aimed to assess GLUT1's function ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Jiehong Li Yijian Li Guanjin Chen Yan Liang Jianpeng Xie Shuiying Zhang Kai Zhong Tong Jiang Haisu Yi Haixiong Tang Xin Chen Source Type: research
Poorly Differentiated Hepatocellular Carcinoma Cells Avoid Apoptosis by Interacting with T Cells via CD40-CD40L Linkage
We examined CD40 levels in HCC patient tissues and various HCC cell lines, studying their interaction with CD4+T cells. RNA sequencing analysis was performed to explore the mechanisms of CD40 induction. Poorly differentiated HCC tumour tissues exhibited high membrane-bound CD40 (mCD40) expression, in contrast to non-tumour areas. Poorly differentiated HCC cell lines showed high expression of mCD40 with low CD40 promoter methylation which were opposite of well-differentiated ones. Solely modulating CD40 expression in HCC cells exerted no direct consequences on cell growth or appearance. Interestingly, HLFs co-cultured with ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hanh Vinh Ngo Thuy Thi Thanh Le Hieu Ngoc Vu Hai Hoang Hiroko Ikenaga Misako Sato-Matsubara Sawako Uchida-Kobayashi Hayato Urushima Khanh Van Nguyen Ha Thi Nguyen Hiroji Shinkawa Shoji Kubo Naoko Ohtani Masaru Enomoto Akihiro Tamori Norifumi Kawada Source Type: research
TGF β1 Drives Integrin Dependent Pericyte Migration and Microvascular Destabilization In Fibrotic Disease
Am J Pathol. 2024 Mar 26:S0002-9440(24)00118-4. doi: 10.1016/j.ajpath.2024.02.021. Online ahead of print.ABSTRACTInteractions between endothelial cells (EC) and mural pericytes (PC) are critical to maintaining the stability and function of the microvascular wall. Abnormal interactions between these two cell types are a hallmark of progressive fibrotic diseases, such as systemic sclerosis (SSc, scleroderma). However, the role that PCs play in signaling microvascular dysfunction remains underexplored. It is hypothesized that integrin-matrix interactions contribute to PC migration from the vascular wall and conversion into in...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Amanda S Pellowe Michelle J Wu Tae-Yun Kang Tracy D Chung Adrian Ledesma-Mendoza Erica Herzog Andre Levchenko Ian Odell John Varga Anjelica L Gonzalez Source Type: research
Enhanced Optic Nerve Expansion and Altered Ultrastructure of Elastic Fibers Induced by Lysyl Oxidase Inhibition in a Mouse Model of Marfan Syndrome
Am J Pathol. 2024 Mar 26:S0002-9440(24)00115-9. doi: 10.1016/j.ajpath.2024.03.002. Online ahead of print.ABSTRACTTwo major constituents of exfoliation material (XFM), fibrillin- 1 and lysyl oxidase-like 1 (encoded by FBN1 and LOXL1) are implicated in exfoliation glaucoma (XFG), yet their individual contributions to ocular phenotype are minor. To test the hypothesis that a combination of FBN1 mutation and LOXL1 deficiency exacerbates ocular phenotypes, pan-LOX inhibitor, β-aminopropionitrile (BAPN) was used to treat adult wild-type (wt) and Fbn1C1041G/+ mice for 8 weeks and their eyes were examined. Although intraocular pr...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hang-Jing Wu Evan Krystofiak John Kuchtey Rachel W Kuchtey Source Type: research
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury
Am J Pathol. 2024 Mar 26:S0002-9440(24)00116-0. doi: 10.1016/j.ajpath.2024.03.003. Online ahead of print.ABSTRACTAcute lung injury (ALI) is a devastating clinical disease caused by different factors, with high morbidity and mortality. It has been shown that lung injury and inflammation caused by lipopolysaccharide (LPS) can be modulated by NLRP3 inflammasome activation, yet its exact function within the airway epithelium is still unknown. Meanwhile, glucose transporter protein 1 (GLUT1) has been shown to contribute to a number of inflammatory illnesses, including ALI. The present study was aimed to assess GLUT1's function ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Jiehong Li Yijian Li Guanjin Chen Yan Liang Jianpeng Xie Shuiying Zhang Kai Zhong Tong Jiang Haisu Yi Haixiong Tang Xin Chen Source Type: research
Poorly Differentiated Hepatocellular Carcinoma Cells Avoid Apoptosis by Interacting with T Cells via CD40-CD40L Linkage
We examined CD40 levels in HCC patient tissues and various HCC cell lines, studying their interaction with CD4+T cells. RNA sequencing analysis was performed to explore the mechanisms of CD40 induction. Poorly differentiated HCC tumour tissues exhibited high membrane-bound CD40 (mCD40) expression, in contrast to non-tumour areas. Poorly differentiated HCC cell lines showed high expression of mCD40 with low CD40 promoter methylation which were opposite of well-differentiated ones. Solely modulating CD40 expression in HCC cells exerted no direct consequences on cell growth or appearance. Interestingly, HLFs co-cultured with ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hanh Vinh Ngo Thuy Thi Thanh Le Hieu Ngoc Vu Hai Hoang Hiroko Ikenaga Misako Sato-Matsubara Sawako Uchida-Kobayashi Hayato Urushima Khanh Van Nguyen Ha Thi Nguyen Hiroji Shinkawa Shoji Kubo Naoko Ohtani Masaru Enomoto Akihiro Tamori Norifumi Kawada Source Type: research
TGF β1 Drives Integrin Dependent Pericyte Migration and Microvascular Destabilization In Fibrotic Disease
Am J Pathol. 2024 Mar 26:S0002-9440(24)00118-4. doi: 10.1016/j.ajpath.2024.02.021. Online ahead of print.ABSTRACTInteractions between endothelial cells (EC) and mural pericytes (PC) are critical to maintaining the stability and function of the microvascular wall. Abnormal interactions between these two cell types are a hallmark of progressive fibrotic diseases, such as systemic sclerosis (SSc, scleroderma). However, the role that PCs play in signaling microvascular dysfunction remains underexplored. It is hypothesized that integrin-matrix interactions contribute to PC migration from the vascular wall and conversion into in...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Amanda S Pellowe Michelle J Wu Tae-Yun Kang Tracy D Chung Adrian Ledesma-Mendoza Erica Herzog Andre Levchenko Ian Odell John Varga Anjelica L Gonzalez Source Type: research
Enhanced Optic Nerve Expansion and Altered Ultrastructure of Elastic Fibers Induced by Lysyl Oxidase Inhibition in a Mouse Model of Marfan Syndrome
Am J Pathol. 2024 Mar 26:S0002-9440(24)00115-9. doi: 10.1016/j.ajpath.2024.03.002. Online ahead of print.ABSTRACTTwo major constituents of exfoliation material (XFM), fibrillin- 1 and lysyl oxidase-like 1 (encoded by FBN1 and LOXL1) are implicated in exfoliation glaucoma (XFG), yet their individual contributions to ocular phenotype are minor. To test the hypothesis that a combination of FBN1 mutation and LOXL1 deficiency exacerbates ocular phenotypes, pan-LOX inhibitor, β-aminopropionitrile (BAPN) was used to treat adult wild-type (wt) and Fbn1C1041G/+ mice for 8 weeks and their eyes were examined. Although intraocular pr...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Hang-Jing Wu Evan Krystofiak John Kuchtey Rachel W Kuchtey Source Type: research
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury
Am J Pathol. 2024 Mar 26:S0002-9440(24)00116-0. doi: 10.1016/j.ajpath.2024.03.003. Online ahead of print.ABSTRACTAcute lung injury (ALI) is a devastating clinical disease caused by different factors, with high morbidity and mortality. It has been shown that lung injury and inflammation caused by lipopolysaccharide (LPS) can be modulated by NLRP3 inflammasome activation, yet its exact function within the airway epithelium is still unknown. Meanwhile, glucose transporter protein 1 (GLUT1) has been shown to contribute to a number of inflammatory illnesses, including ALI. The present study was aimed to assess GLUT1's function ...
Source: Am J Pathol - March 28, 2024 Category: Pathology Authors: Jiehong Li Yijian Li Guanjin Chen Yan Liang Jianpeng Xie Shuiying Zhang Kai Zhong Tong Jiang Haisu Yi Haixiong Tang Xin Chen Source Type: research
