Diabetes accelerates steatohepatitis in mice: liver pathology and single-cell gene expression signatures
Am J Pathol. 2024 Feb 1:S0002-9440(24)00037-3. doi: 10.1016/j.ajpath.2024.01.007. Online ahead of print.ABSTRACTGlucose lowering independently reduces liver fibrosis in human NAFLD. We investigated the impact of diabetes on steatohepatitis and established a novel mouse model for diabetic steatohepatitis. Male C57BL/6J mice were fed a 60% high-fat diet (HFD) and injected with carbon tetrachloride (CCl4) and streptozotocin (STZ) to induce diabetes. The HFD+CCl4+STZ group displayed more severe liver steatosis, hepatocyte ballooning, and regenerative nodules compared to other groups. Diabetes upregulated inflammatory cytokine-...
Source: Am J Pathol - February 3, 2024 Category: Pathology Authors: Tuerdiguli Abuduyimiti Hisanori Goto Kumi Kimura Yu Oshima Ryota Tanida Kyoko Kamoshita Nontaphat Leerach Halimulati Abuduwaili Hein Ko Oo Qifang Li Cynthia M Galicia-Medina Hiroaki Takayama Kiyo-Aki Ishii Yujiro Nakano Yumie Takeshita Tomohiro Iba Hisami Source Type: research
Neutrophil Infiltration and Function in the Pathogenesis of Inflammatory Airspace Disease
Am J Pathol. 2024 Feb 1:S0002-9440(24)00033-6. doi: 10.1016/j.ajpath.2023.12.008. Online ahead of print.ABSTRACTNeutrophils are an important cell type often considered the body's first responder to inflammatory insult or damage. They are recruited to the tissue of the lungs in patients with inflammatory airspace diseases and have unique and complex functions that range from helpful to harmful. The uniqueness of these functions is due to the heterogeneity of the inflammatory cascade and retention in the vasculature. Neutrophils are known to marginate, or remain stagnant, in the lungs even in nondisease conditions. This revi...
Source: Am J Pathol - February 3, 2024 Category: Pathology Authors: Maureen E Haynes David P Sullivan William A Muller Source Type: research
Omics Approaches Unveiling the Biology of Human Atherosclerotic Plaques
Am J Pathol. 2024 Jan 25:S0002-9440(24)00005-1. doi: 10.1016/j.ajpath.2023.12.007. Online ahead of print.ABSTRACTAtherosclerosis is a chronic inflammatory disease of the arterial wall, characterized by the buildup of plaques with the accumulation and transformation of lipids, immune cells, vascular smooth muscle cells, and necrotic cell debris. Plaques with collagen-poor thin fibrous caps infiltrated by macrophages and lymphocytes are considered unstable because they are at the greatest risk of rupture and clinical events. However, the current histologic definition of plaque types may not fully capture the complex molecula...
Source: Am J Pathol - January 27, 2024 Category: Pathology Authors: Xun Wu Hanrui Zhang Source Type: research
Macrophage Activation in Synovitis and Osteoarthritis of Temporomandibular Joint and Its Relationship with the Progression of Synovitis and Bone Remodeling
This study investigates the regulatory mechanisms of synovial macrophages and their polarization in the progression of temporomandibular joint osteoarthritis (TMJOA). Macrophage depletion models were established by intra-articular injection of clodronate liposomes and unloaded liposomes. TMJOA was induced by intra-articular injection of 50 μL Complete Freund's Adjuvant and the surgery of disc perforation. The contralateral joint was used as the control group. The expression of F4/80, CD86, and CD206 in the synovium was detected by immunofluorescence staining analysis. Hematoxylin and eosin staining and TMJOA synovial scor...
Source: Am J Pathol - January 20, 2024 Category: Pathology Authors: Shiyu Hu Huimin Li Henghua Jiang Xin Liu Jin Ke Xing Long Source Type: research
Chronic Sleep Deprivation Impairs Visual Functions via Oxidative Damage in Mice
Am J Pathol. 2024 Feb;194(2):307-320. doi: 10.1016/j.ajpath.2023.11.004.ABSTRACTSleep deprivation (SD) is a global public health burden, and has a detrimental role in the nervous system. Retina is an important part of the central nervous system; however, whether SD affects retinal structures and functions remains largely unknown. Herein, chronic SD mouse model indicated that loss of sleep for 4 months could result in reductions in the visual functions, but without obvious morphologic changes of the retina. Ultrastructural analysis by transmission electron microscope revealed the deterioration of mitochondria, which was acc...
Source: Am J Pathol - January 20, 2024 Category: Pathology Authors: Liying Tang Houjian Zhang Yi Liao Shengmei Zhou Yaqiong Yang Mouxin Zhang Yuli Guo Tingyu Xie Shikun Chen Weijie Ouyang Xiang Lin Shaopan Wang Caihong Huang Minjie Zhang Jingbin Zhuang Jiankai Zhao Rongrong Zhang Changjun Zhang Zibing Jin Jiaoyue Hu Zuguo Source Type: research
Correction
Am J Pathol. 2024 Feb;194(2):321. doi: 10.1016/j.ajpath.2023.12.002. Epub 2023 Dec 13.NO ABSTRACTPMID:38245253 | DOI:10.1016/j.ajpath.2023.12.002 (Source: Am J Pathol)
Source: Am J Pathol - January 20, 2024 Category: Pathology Source Type: research
IL-22 Binding Protein/IL-22 Axis in Regulating Acute Lung Injury
Am J Pathol. 2024 Jan 8:S0002-9440(24)00003-8. doi: 10.1016/j.ajpath.2024.01.002. Online ahead of print.NO ABSTRACTPMID:38199431 | DOI:10.1016/j.ajpath.2024.01.002 (Source: Am J Pathol)
Source: Am J Pathol - January 10, 2024 Category: Pathology Authors: Amber Pillar Md Khadem Ali Source Type: research
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease
Am J Pathol. 2024 Jan 1:S0002-9440(23)00487-X. doi: 10.1016/j.ajpath.2023.12.006. Online ahead of print.ABSTRACTDespite significant advances in medical treatments and drug development, atherosclerotic cardiovascular disease (ASCVD) remains a leading cause of death worldwide. Dysregulated lipid metabolism is a well-established driver of ASCVD. Unfortunately, even with potent lipid-lowering therapies, ASCVD-related deaths have continued to increase over the past decade, highlighting an incomplete understanding of the underlying risk factors and mechanisms of ASCVD. Accumulating evidence over the past decades indicates a corr...
Source: Am J Pathol - January 3, 2024 Category: Pathology Authors: Sumit Kumar Anand Theresea-Anne Governale Xiangyu Zhang Babak Razani Arif Yurdagul Christopher B Pattillo Oren Rom Source Type: research
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease
Am J Pathol. 2024 Jan 1:S0002-9440(23)00487-X. doi: 10.1016/j.ajpath.2023.12.006. Online ahead of print.ABSTRACTDespite significant advances in medical treatments and drug development, atherosclerotic cardiovascular disease (ASCVD) remains a leading cause of death worldwide. Dysregulated lipid metabolism is a well-established driver of ASCVD. Unfortunately, even with potent lipid-lowering therapies, ASCVD related deaths have continued to increase over the last decade, highlighting an incomplete understanding of the underlying risk factors and mechanisms of ASCVD. Accumulating evidence over the last decades indicates a corr...
Source: Am J Pathol - January 3, 2024 Category: Pathology Authors: Sumit Kumar Anand Theresea-Anne Governale Xiangyu Zhang Babak Razani Arif Yurdagul Christopher B Pattillo Oren Rom Source Type: research
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease
Am J Pathol. 2024 Jan 1:S0002-9440(23)00487-X. doi: 10.1016/j.ajpath.2023.12.006. Online ahead of print.ABSTRACTDespite significant advances in medical treatments and drug development, atherosclerotic cardiovascular disease (ASCVD) remains a leading cause of death worldwide. Dysregulated lipid metabolism is a well-established driver of ASCVD. Unfortunately, even with potent lipid-lowering therapies, ASCVD related deaths have continued to increase over the last decade, highlighting an incomplete understanding of the underlying risk factors and mechanisms of ASCVD. Accumulating evidence over the last decades indicates a corr...
Source: Am J Pathol - January 3, 2024 Category: Pathology Authors: Sumit Kumar Anand Theresea-Anne Governale Xiangyu Zhang Babak Razani Arif Yurdagul Christopher B Pattillo Oren Rom Source Type: research
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease
Am J Pathol. 2024 Jan 1:S0002-9440(23)00487-X. doi: 10.1016/j.ajpath.2023.12.006. Online ahead of print.ABSTRACTDespite significant advances in medical treatments and drug development, atherosclerotic cardiovascular disease (ASCVD) remains a leading cause of death worldwide. Dysregulated lipid metabolism is a well-established driver of ASCVD. Unfortunately, even with potent lipid-lowering therapies, ASCVD related deaths have continued to increase over the last decade, highlighting an incomplete understanding of the underlying risk factors and mechanisms of ASCVD. Accumulating evidence over the last decades indicates a corr...
Source: Am J Pathol - January 3, 2024 Category: Pathology Authors: Sumit Kumar Anand Theresea-Anne Governale Xiangyu Zhang Babak Razani Arif Yurdagul Christopher B Pattillo Oren Rom Source Type: research
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease
Am J Pathol. 2024 Jan 1:S0002-9440(23)00487-X. doi: 10.1016/j.ajpath.2023.12.006. Online ahead of print.ABSTRACTDespite significant advances in medical treatments and drug development, atherosclerotic cardiovascular disease (ASCVD) remains a leading cause of death worldwide. Dysregulated lipid metabolism is a well-established driver of ASCVD. Unfortunately, even with potent lipid-lowering therapies, ASCVD related deaths have continued to increase over the last decade, highlighting an incomplete understanding of the underlying risk factors and mechanisms of ASCVD. Accumulating evidence over the last decades indicates a corr...
Source: Am J Pathol - January 3, 2024 Category: Pathology Authors: Sumit Kumar Anand Theresea-Anne Governale Xiangyu Zhang Babak Razani Arif Yurdagul Christopher B Pattillo Oren Rom Source Type: research
Anti-Angiogenic and Anti-Scarring Dual Effect of Galectin-3 Inhibition in Mouse Animal Models of Corneal Wound Healing
In this study, using mouse models of corneal wound healing, we demonstrate that topical application of a small molecule inhibitor of galectin-3, GB1265: reduces (i) corneal angiogenesis, (ii) corneal fibrosis, (iii) infiltration of immune cells and (iv) expression of proinflammatory cytokine, IL-1β, in injured corneas. Four independent techniques (RNA-seq, NanoString, RT-qPCR, and Western blot analyses) all demonstrated that decrease in corneal opacity in the galectin-3 inhibitor-treated corneas is associated with a decrease in a number of genes and signaling pathways that are known to promote fibrosis. These findings all...
Source: Am J Pathol - December 30, 2023 Category: Pathology Authors: Zhiyi Cao Abdulraouf Ramadan Albert Tai Fredrik Zetterberg Noorjahan Panjwani Source Type: research
Mammalian Target of Rapamycin Complex 1 Activation in Macrophages Contributes to Persistent Lung Inflammation following Respiratory Tract Viral Infection
Am J Pathol. 2023 Dec 28:S0002-9440(23)00485-6. doi: 10.1016/j.ajpath.2023.11.017. Online ahead of print.ABSTRACTRespiratory tract virus infections cause millions of hospitalizations worldwide each year. Severe infections lead to lung damage that coincides with persistent inflammation and a lengthy repair period. Vaccination and antiviral therapy help to mitigate severe infections before or during the acute stage of disease, but there are currently limited specific treatment options available to individuals experiencing the long-term sequelae of respiratory viral infection. C57BL/6 mice were infected with influenza A/PR/8/...
Source: Am J Pathol - December 30, 2023 Category: Pathology Authors: Brydie R Huckestein Kelly Zeng Rosemary Westcott Jonathan K Alder Danielle Antos Jay K Kolls John F Alcorn Source Type: research
Anti-Angiogenic and Anti-Scarring Dual Effect of Galectin-3 Inhibition in Mouse Animal Models of Corneal Wound Healing
In this study, using mouse models of corneal wound healing, we demonstrate that topical application of a small molecule inhibitor of galectin-3, GB1265: reduces (i) corneal angiogenesis, (ii) corneal fibrosis, (iii) infiltration of immune cells and (iv) expression of proinflammatory cytokine, IL-1β, in injured corneas. Four independent techniques (RNA-seq, NanoString, RT-qPCR, and Western blot analyses) all demonstrated that decrease in corneal opacity in the galectin-3 inhibitor-treated corneas is associated with a decrease in a number of genes and signaling pathways that are known to promote fibrosis. These findings all...
Source: Am J Pathol - December 30, 2023 Category: Pathology Authors: Zhiyi Cao Abdulraouf Ramadan Albert Tai Fredrik Zetterberg Noorjahan Panjwani Source Type: research
