Therapeutic Potential of Stem Cells in Natural Killer-Like B Cell-Associated Diseases
Adv Exp Med Biol. 2024 Feb 29. doi: 10.1007/5584_2024_799. Online ahead of print.ABSTRACTStem cells are undifferentiated cells possessing a remarkable capacity to develop into multiple cell types. NKB cells, referred to "natural killer-like B cells," are recently identified subtype of B lymphocytes possessing characteristics that are similar to both natural killer (NK) cells and regular B lymphocytes. NK cells are lymphocyte-like in structure and cytotoxic in nature participating in the immediate immune response to the infected or malignant cells, whereas B lymphocytes produce antibodies and participate in adaptive immune ...
Source: Advances in Experimental Medicine and Biology - February 28, 2024 Category: Research Authors: Manali Rupareliya Pravin Shende Source Type: research

Isolation of Extracellular Vesicles Using Titanium Dioxide Microspheres
This study describes a comprehensive workflow for the isolation and characterization of plasma extracellular vesicles (EVs) using mass spectrometry-based proteomics techniques. The aim of this chapter is describe the EV isolation using TiO2 beads enrichment and high-throughput mass spectrometry techniques to efficiently identify the protein composition of EVs in a fast and straightforward manner.PMID:38409413 | DOI:10.1007/978-3-031-50624-6_1 (Source: Advances in Experimental Medicine and Biology)
Source: Advances in Experimental Medicine and Biology - February 27, 2024 Category: Research Authors: Veronica Feijoli Santiago Livia Rosa-Fernandes Janaina Macedo-da-Silva Claudia B Angeli Simon Ngao Mule Claudio R F Marinho Ana Claudia Torrecilhas Suely N K Marie Giuseppe Palmisano Source Type: research

Glycosort: A Computational Solution to Post-process Quantitative Large-Scale Intact Glycopeptide Analyses
Adv Exp Med Biol. 2024;1443:23-32. doi: 10.1007/978-3-031-50624-6_2.ABSTRACTProtein glycosylation is a post-translational modification involving the addition of carbohydrates to proteins and plays a crucial role in protein folding and various biological processes such as cell recognition, differentiation, and immune response. The vast array of natural sugars available allows the generation of plenty of unique glycan structures in proteins, adding complexity to the regulation and biological functions of glycans. The diversity is further increased by enzymatic site preferences and stereochemical conjugation, leading to an im...
Source: Advances in Experimental Medicine and Biology - February 27, 2024 Category: Research Authors: Lucas C Lazari Veronica Feijoli Santiago Gilberto S de Oliveira Simon Ngao Mule Claudia B Angeli Livia Rosa-Fernandes Giuseppe Palmisano Source Type: research

Applications of Mass Spectrometry in the Characterization, Screening, Diagnosis, and Prognosis of COVID-19
Adv Exp Med Biol. 2024;1443:33-61. doi: 10.1007/978-3-031-50624-6_3.ABSTRACTMass spectrometry (MS) is a powerful analytical technique that plays a central role in modern protein analysis and the study of proteostasis. In the field of advanced molecular technologies, MS-based proteomics has become a cornerstone that is making a significant impact in the post-genomic era and as precision medicine moves from the research laboratory to clinical practice. The global dissemination of COVID-19 has spurred collective efforts to develop effective diagnostics, vaccines, and therapeutic interventions. This chapter highlights how MS s...
Source: Advances in Experimental Medicine and Biology - February 27, 2024 Category: Research Authors: Camila Akemi Oliveira Yamada Bruno de Paula Oliveira Santos Rafael Pereira Lemos Ana Carolina Silva Batista Izabela Mamede C A da Concei ção Adriano de Paula Sabino Lu ís Maurício Trambaioli da Rocha E Lima Mariana T Q de Magalh ães Source Type: research

Proteomics and Metabolomics in Congenital Zika Syndrome: A Review of Molecular Insights and Biomarker Discovery
Adv Exp Med Biol. 2024;1443:63-85. doi: 10.1007/978-3-031-50624-6_4.ABSTRACTZika virus (ZIKV) infection can be transmitted vertically, leading to the development of congenital Zika syndrome (CZS) in infected fetuses. During the early stages of gestation, the fetuses face an elevated risk of developing CZS. However, it is important to note that late-stage infections can also result in adverse outcomes. The differences between CZS and non-CZS phenotypes remain poorly understood. In this review, we provide a summary of the molecular mechanisms underlying ZIKV infection and placental and blood-brain barriers trespassing. Also,...
Source: Advances in Experimental Medicine and Biology - February 27, 2024 Category: Research Authors: Patricia Sosa-Acosta F ábio C S Nogueira Gilberto B Domont Source Type: research

Mass Spectrometry Applications to Study Human Microbiome
Adv Exp Med Biol. 2024;1443:87-101. doi: 10.1007/978-3-031-50624-6_5.ABSTRACTMicrobiotas are an adaptable component of ecosystems, including human ecology. Microorganisms influence the chemistry of their specialized niche, such as the human gut, as well as the chemistry of distant surroundings, such as other areas of the body. Metabolomics based on mass spectrometry (MS) is one of the primary methods for detecting and identifying small compounds generated by the human microbiota, as well as understanding the functional significance of these microbial metabolites. This book chapter gives basic knowledge on the kinds of unta...
Source: Advances in Experimental Medicine and Biology - February 27, 2024 Category: Research Authors: Mohamed A Raslan Sara A Raslan Eslam M Shehata Amr S Mahmoud Marcus Vinicius Can ário Viana Fl ávia Aburjaile Debmalya Barh Nagwa A Sabri Vasco Azevedo Source Type: research