Genetic diversity analysis of grapevine rupestris stem pitting-associated virus from grapevine by colony PCR-SSCP and -RFLP
Acta Virol. 2022;66(1):85-89. doi: 10.4149/av_2022_106.ABSTRACTWe have developed methods for detecting the genetic diversity of grapevine rupestris stem pitting-associated virus (GRSPaV) based on restriction fragment length polymorphism (RFLP) and single stranded conformational polymorphism (SSCP) in the 905 nt 3' sequence. The amplicons were cloned from six grapevine cultivars, and colony polymerase chain reaction (colony PCR) using recombination bacteria was subsequently analyzed by RFLP and SSCP. Four haplotypes of SSCP and six haplotypes of Sac I RFLPs were defined. The two methods had a 40% discrepancy rate in showing...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Guo-Jun Hu Hong-Juan Zhu Zun-Ping Zhang Xu-Dong Fan Fang Ren Ya-Feng Dong Source Type: research

A  novel approach for estimation of anti-FMDV protective immunity generated in multiple vaccinated field animals
In this study, forty serum samples from field buffaloes vaccinated with inactivated foot-and-mouth disease (FMD) vaccine were collected. These animals were multiple vaccinated with the above vaccine during previous years. The study was conducted to determine the actual status of the protective antibodies generated after vaccination. Initially, the serum samples were tested by Liquid phase blocking ELISA (LPBE), and only samples with titer more than 1.4 in LPBE were chosen for further analysis. These samples were tested with an in-house Gold Nanoparticle-based test for detection of anti-FMDV structural protein antibodies, i...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Beenu Jain Anuj Tewari Minakshi Prasad Surender Kumar Kadian Source Type: research

Suitability of different plant species for experimental agroinfection with Plum pox virus-based expression vector for potential production of edible vaccines
Acta Virol. 2022;66(1):95-97. doi: 10.4149/av_2022_111.ABSTRACTNine herbaceous plant species were tested for susceptibility to Plum pox virus (PPV) by Agrobacterium-mediated delivery of its infectious cDNA clone. Two of them became infected, namely spinach (local infection) and oilseed poppy (systemic infection). As a control, PPV infection was successfully established in plum seedlings following agroinfiltration, thus providing the first report of agroinfection in Prunus species. According to our results, oilseed poppy can be considered as a candidate host for the production of edible vaccines by a PPV-derived expression ...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Adam Achs Miroslav Glasa Peter Alaxin Zdeno W Šubr Source Type: research

Molecular identification and characterization of badnaviruses infecting sugarcane in Ethiopia
This study was aimed to identify and characterize SCBV isolates in Ethiopia using molecular methods. Out of 292 leaf samples collected and tested by PCR, 76 samples (26% incidence level) were found SCBV-positive. Nucleotide sequence analysis results showed that three Ethiopian isolates (SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10) shared high level of nucleotide identity (99.5-100%) among themselves and with SCBV isolates from China (accession numbers MH037614 and MH037915). Another isolate, SCBV-EtC2, shared maximum identity of 78% with the other three SCBV isolates from Ethiopia and 99.8% with SCBV isolates from China (KM214357 ...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Simatsidk Haregu Dawit Kidanemariam Adane Abraham Source Type: research

Transcriptomic analyses reveal highly conserved plant amalgavirus genomes in different species of Allium
Acta Virol. 2022;66(1):11-17. doi: 10.4149/av_2022_102.ABSTRACTThis work aims to study amalgavirus diversity in different species of allium collected around the world. Transcriptomic data of 19 Sequence Read Archive runs available at GenBank, as well as RNA-seq data generated from onion tissue from fields in Brazil were used to assemble nine allium cepa amalgavirus 1 (AcAV-1) and nine allium cepa amalgavirus 2 (AcAV-2) genomes from different species of allium worldwide. Sequence demarcation tool analyses of RdRp amino acid sequences revealed identities above 99% within each species, except for an isolate of AcAV-1 from All...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Jos é Ailton Cruz Macedo Dos Santos Adriano M árcio Freire Silva J úlio Carlos Polimeni de Mesquita Rosana Blawid Source Type: research

Comparative analysis of HBV basic core promoter/pre-core gene mutations and viral quasispecies diversity in HIV/HBV co-infected and HBV mono-infected patients
In this study, a total of 230 patients were recruited, and 199 patients whose HBV BCP/preC gene were successfully amplified and sequenced, including 99 HIV/HBV co-infected and 100 HBV mono-infected patients. Next-generation sequencing was used for detection of BCP/preC mutations which were then compared in patients with different HBV genotypes and different HBeAg statuses, and 1% and 20% cutoff values were defined to evaluate the mutations. HBV quasispecies diversity was also compared in HIV/HBV co-infected and HBV mono-infected patients. Among the patients infected with HBV genotype C and HBeAg-negative status, the freque...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Haohui Deng Hongbo Gao Yu Liu Ying Xu Juncheng Yang Miaoxian Zhao Huiyuan Liu Zhanhui Wang Source Type: research

Genomic phylogenetic analyses of four major hand, foot and mouth disease-related enteroviruses
In this study, we performed the phylogenetic analyses of the EVA71, CVA16, CVA6 and CVA10 using available full-length genomic sequences. We found that the topologies of phylogenetic trees of full-length genomic sequences and VP1 sequences were almost consistent, except few subtypes of EVA71 and CVA10. The mean genetic divergence was 15.8-27% between subtypes and less than 12% within subtypes/sub-subtypes at genomic level. Comparison of phylogenetic topologies between genomic and VP1 sequences helped us to identify two new EVA71 inter-subtype recombinants RF01_CC4 and RF02_CC4. Furthermore, EVA71 subtypes C1 and C2 and CVA1...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Yajuan Dong Zhenzhou Wan Shenwei Li Jian-Hua Wang Xia Jin Guoying Yu Chiyu Zhang Source Type: research

Interaction between proteins of the PPAR γ and NFκB immune response pathways and rotavirus non-structural proteins
Acta Virol. 2022;66(1):39-54. doi: 10.4149/av_2022_105.ABSTRACTCells infected with MA104 rotavirus and/or transfected with plasmids expressing NSP proteins, were analyzed for expression of cellular proteins related to NFκB and PPARγ pathways and evaluated through the ELISA, luminescence, flow cytometry and Western blot techniques. The association between cellular and viral (NSPs) proteins was examined by ELISA, epifluorescence and confocal microscopy techniques. It was observed that NSP1 protein interacts with RXR, NSP1, and NSP3 with PPARγ, NSP2 with p-IKKα/β and NSP5 with NFκB proteins. We have found that phosphory...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Dory G ómez Carlos A Guerrero Source Type: research

RPS3-induced antiviral cytokines inhibit the proliferation of classical swine fever virus
Acta Virol. 2022;66(1):55-64. doi: 10.4149/av_2022_107.ABSTRACTClassical swine fever virus (CSFV) infection results in serious economic losses to the pig industry. This positive-sense RNA virus hijacks cell host proteins for its own replication. Although previous studies have shown that RPS3, a 40S ribosomal subunit protein, is mainly required for DNA repair, apoptosis and inflammation, the effect of RPS3 on CSFV replication remains uncertain. Thus, we investigated the potential role of RPS3 in CSFV infection in RPS3-knockdown and -overexpressing cell lines using real-time fluorescence quantitative PCR (RT-qPCR) and indire...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Di Zhao Liang Zhang Mengzhao Song Yanming Zhang Mail Source Type: research

nH115a, a  novel inhibitor of the La protein: Effect on expression of multiple RNAs in hepatitis B virus-infected hepatoma cells and embryotoxicity profile
In conclusion, immune responses, TGF-β/SMAD signaling, and cancer-related pathways may function in the nH115a-mediated inhibition of HBV replication. Keywords: hepatitis B virus; La protein; inhibitor; nH115a.PMID:35380866 | DOI:10.4149/av_2022_108 (Source: Acta Virologica)
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Jiaqian Pan Jing Tang Source Type: research

Acetylation of late expression factor 4 is crucial for the transcription and proliferation of Bombyx mori nucleopolyhedrovirus
Acta Virol. 2022;66(1):77-84. doi: 10.4149/av_2022_109.ABSTRACTLate expression factor 4 (LEF4), RNA polymerase subunit of Bombyx mori nucleopolyhedrovirus (BmNPV), plays an enzymatic role to enhance the capping of pre-mRNA of late and very late genes. Lysine acetylation is a post-translational modification process having many important functions associated with the regulation of a gene expression. Our previous study on lysine acetylome in BmNPV infected BmN cells showed that LEF 4 was acetylated at lysine 76 (K76). However, it is still unclear whether the modification of K76 residue contributes to the modulation of viral g...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Jonas Ngowo Xu Gao Yajie Zhu Fuxiang Mao Jihai Lei Enoch Obeng Wei Yu Source Type: research

Genetic diversity analysis of grapevine rupestris stem pitting-associated virus from grapevine by colony PCR-SSCP and -RFLP
Acta Virol. 2022;66(1):85-89. doi: 10.4149/av_2022_106.ABSTRACTWe have developed methods for detecting the genetic diversity of grapevine rupestris stem pitting-associated virus (GRSPaV) based on restriction fragment length polymorphism (RFLP) and single stranded conformational polymorphism (SSCP) in the 905 nt 3' sequence. The amplicons were cloned from six grapevine cultivars, and colony polymerase chain reaction (colony PCR) using recombination bacteria was subsequently analyzed by RFLP and SSCP. Four haplotypes of SSCP and six haplotypes of Sac I RFLPs were defined. The two methods had a 40% discrepancy rate in showing...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Guo-Jun Hu Hong-Juan Zhu Zun-Ping Zhang Xu-Dong Fan Fang Ren Ya-Feng Dong Source Type: research

A  novel approach for estimation of anti-FMDV protective immunity generated in multiple vaccinated field animals
In this study, forty serum samples from field buffaloes vaccinated with inactivated foot-and-mouth disease (FMD) vaccine were collected. These animals were multiple vaccinated with the above vaccine during previous years. The study was conducted to determine the actual status of the protective antibodies generated after vaccination. Initially, the serum samples were tested by Liquid phase blocking ELISA (LPBE), and only samples with titer more than 1.4 in LPBE were chosen for further analysis. These samples were tested with an in-house Gold Nanoparticle-based test for detection of anti-FMDV structural protein antibodies, i...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Beenu Jain Anuj Tewari Minakshi Prasad Surender Kumar Kadian Source Type: research

Suitability of different plant species for experimental agroinfection with Plum pox virus-based expression vector for potential production of edible vaccines
Acta Virol. 2022;66(1):95-97. doi: 10.4149/av_2022_111.ABSTRACTNine herbaceous plant species were tested for susceptibility to Plum pox virus (PPV) by Agrobacterium-mediated delivery of its infectious cDNA clone. Two of them became infected, namely spinach (local infection) and oilseed poppy (systemic infection). As a control, PPV infection was successfully established in plum seedlings following agroinfiltration, thus providing the first report of agroinfection in Prunus species. According to our results, oilseed poppy can be considered as a candidate host for the production of edible vaccines by a PPV-derived expression ...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Adam Achs Miroslav Glasa Peter Alaxin Zdeno W Šubr Source Type: research

Molecular identification and characterization of badnaviruses infecting sugarcane in Ethiopia
This study was aimed to identify and characterize SCBV isolates in Ethiopia using molecular methods. Out of 292 leaf samples collected and tested by PCR, 76 samples (26% incidence level) were found SCBV-positive. Nucleotide sequence analysis results showed that three Ethiopian isolates (SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10) shared high level of nucleotide identity (99.5-100%) among themselves and with SCBV isolates from China (accession numbers MH037614 and MH037915). Another isolate, SCBV-EtC2, shared maximum identity of 78% with the other three SCBV isolates from Ethiopia and 99.8% with SCBV isolates from China (KM214357 ...
Source: Acta Virologica - April 5, 2022 Category: Infectious Diseases Authors: Simatsidk Haregu Dawit Kidanemariam Adane Abraham Source Type: research