Instrument-free, visual and direct detection of porcine reproductive and respiratory syndrome viruses in resource-limited settings
In this study, a direct colorimetric RT-loop-mediated isothermal amplification (RT-LAMP) method was developed to specifically and rapidly detect PRRSV. The RT-LAMP outcomes can be visualized by the naked eye after 45 min of incubation at 65˚C without any cross-reactivity recorded with the bacteria and other viruses tested. In particular, the mobile, non-instrumented, commercial pocket hand warmers were demonstrated to su-ccessfully provide constant temperature for consistent nucleic acid amplification throughout the RT-LAMP reactions. The limit of detection of the assay was defined as the genomic RNA concentration extract...
Source: Acta Virologica - March 23, 2023 Category: Infectious Diseases Authors: Diem Hong Tran Ngan Anh Ngoc Hau Thi Tran Trang Nguyen Minh Thi Bich Ngoc Van Tam Nguyen Van Phan Le Huong Thi Thu Source Type: research
Attenuation of equine herpesvirus 1 through deletion of gE gene and its pathological evaluation in murine model
Acta Virol. 2023;67(1):79-90. doi: 10.4149/av_2023_108.ABSTRACTEquine herpesvirus 1 (EHV1) infection is a global health problem in equines and the virus is responsible for abortions, respiratory disease and myeloencephalitis in horses. Disease management requires proper biosecurity and immunoprophylactic measures. Vaccines strengthening both arms of immunity are essential for proper control and there has been a continuous focus in this area for generation of better vaccines. Here we report construction of bacterial artificial chromosome (BAC) clone of EHV-1 strain Tohana for mutagenesis of the virus and generation of gE ge...
Source: Acta Virologica - March 23, 2023 Category: Infectious Diseases Authors: Bidhan Chandra Bera Taruna Anand Selvaraj Pavulraj Venkataramireddy Balena Stephanie Pradhan Raj Kumar Singh Bhupendra Nath Tripathi Nitin Virmani Source Type: research
High resolution melting curve analysis for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants
Acta Virol. 2023;67(1):91-98. doi: 10.4149/av_2023_109.ABSTRACTSince the emergence of the original Wuhan SARS-CoV-2 strain, several new variants of the virus have emerged. Alpha, Beta, Gamma, Delta and the most recent Omicron variants have been introduced during this pandemic. Several methods including, but not restricted to, allele-specific PCR, ligation with rolling circle amplification and real-time PCR with allele-specific probes are able to detect mutations as low as a single nucleotide polymorphism. High-resolution melting curve analysis is ano-ther technique to assess any mutations in a nucleic acid chain. Confirmed...
Source: Acta Virologica - March 23, 2023 Category: Infectious Diseases Authors: Seyed Jalal Kiani Mehdi Ramshini Farah Bokharaei-Salim Tahereh Donyavi Babak Eshrati Majid Khoshmirsafa Saied Ghorbani Ahmad Tavakoli Seyed Hamidreza Monavari Zohreh Yousefi Ghalejoogh Mohammad Abbasi-Kolli Source Type: research
Kinetics of interferon- λ and receptor expression in response to in vitro respiratory viral infection
In this report, we analyzed the expression of IFNs and some ISGs (MxA, PKR, OAS-1, IFIT-1, RIG-1, MDA5, SOCS-1) in alveolar epithelial cells (A549) in response to infection with influenza A viruses (A/California/07/09 (H1N1pdm); A/Texas/50/12 (H3N2)); influenza B virus (B/Phuket/3073/13); adenovirus type 5 and 6; or respiratory syncytial virus (strain A2). Influenza B virus had the ability to most rapidly induce IFNs and ISGs as well as to stimulate excessive IFN-α, IFN-β and IFN-λ secretion. It seems curious that IAV H1N1pdm did not induce IFN-λ secretion, but enhanced type I IFN and interleukin (IL)-6 production. We ...
Source: Acta Virologica - March 23, 2023 Category: Infectious Diseases Authors: Alexey A Lozhkov Nikita D Yolshin Irina L Baranovskaya Marina A Plotnikova Maria V Sergeeva Natalia E Gyulikhandanova Sergey A Klotchenko Andrey V Vasin Source Type: research
A novel deltacryptic virus identified in Allium cepa from Brazil
Acta Virol. 2023;67(1):109-113. doi: 10.4149/av_2023_111.ABSTRACT This work describes a novel partitivirus genome assembled from RNA-seq data generated from onion tissue from fields in Brazil. A new partitivirus genome composed of three dsRNAs, which was closely related to arhar cryptic virus 1, was assembled from Allium cepa samples from Brazil. The genomic sequences were also identified from available transcriptomic datasets of onion samples from China, Czech Republic, India, South Korea and USA. According to the species demarcation in the Partitiviridae family, the new virus was classified into the genus Deltapartitivir...
Source: Acta Virologica - March 23, 2023 Category: Infectious Diseases Authors: Jos é Ailton Cruz Adriano M árcio Freire J úlio Carlos Polimeni Rosana Blawid Source Type: research
