Simultaneous determination of macrolides in water samples by solid-phase extraction and capillary electrophoresis
Acta Pharm. 2023 Dec 26;73(4):515-535. doi: 10.2478/acph-2023-0041. Print 2023 Dec 1.ABSTRACTSolid-phase extraction (SPE) coupled with capillary electrophoresis (CE) for the determination of macrolide antibiotics (azithromycin, clarithromycin, roxithromycin, tylosin) and tiamulin in water samples was described in this article. These compounds were extracted with different types of sorbents ( Oasis HLB, C18, C8, SDB, and Strata-X) and different masses of sorbents (60 mg, 200 mg, and 500 mg) using different organic solvents (methanol, ethanol, and acetonitrile) and different pH values of water samples (pH 7.00, 8.00, and 9.0...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Dragana Mutavd žić Pavlović Sandra Babi ć Mirta Čizmić Miranda Serti ć Tea Pinu šić Source Type: research

Prescribers' approval rate of pharmacist-initiated interventions to optimise patients' clinical status of hypertension in the ambulatory care setting
Acta Pharm. 2023 Dec 26;73(4):723-734. doi: 10.2478/acph-2023-0047. Print 2023 Dec 1.ABSTRACTThis perspective, pre- and post-intervention study with a one-year follow-up primarily aimed to ascertain prescribers' approval rate of pharmacists' interventions and clinical status of hypertension following comprehensive medication management (CMM) intervention in the ambulatory care clinic. Between January 2018 and January 2022 overall 100 patients with hypertension and other comorbidities were referred to the CMM services at the Health Centre Zagreb - Centar (HCZC). Out of 275 interventions directed to prescribers, 73.1 % of in...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Andrea Brajkovi ć Lucija Ana Bi ćanić Helena Orehova čki Ingrid Prka čin Djenane Ramalho De Oliveira Iva Mucalo Source Type: research

Mendeleev's discovery of the periodic table and the first European Academy of Sciences to honour him
Acta Pharm. 2023 Dec 26;73(4):735-753. doi: 10.2478/acph-2023-0039. Print 2023 Dec 1.ABSTRACTThe chemical science community will commemorate the 155th anniversary of Mendeleev's groundbreaking discovery of the periodic table of elements in 2024. This paper aims to underscore the significance of Mendeleev's honorary membership in the Academy of Sciences in Zagreb, Croatia, which occurred in 1882, making it the first scientific academy in Europe to extend this recognition. Additionally, we seek to explore the contextual circumstances that contributed to this noteworthy event within the broader European context. To provide in...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Vanja Horvat Flegar Suzana Ini ć Source Type: research

Size exclusion chromatography as green support for forced degradation study of adalimumab
Acta Pharm. 2023 Dec 26;73(4):709-721. doi: 10.2478/acph-2023-0044. Print 2023 Dec 1.ABSTRACTSize exclusion chromatography (SEC) has become a powerful tool for analysing size variants of biologic drugs in their native form. Modern SEC can be defined by the use of chromatographic columns packed with sub-3 µm particles, allowing an increase in method throughput compared to that of conventional SEC. We performed the forced degradation study of adalimumab, the first genetically engineered fully humanised immunoglobulin G1 monoclonal antibody, and evaluated tha possibilities of an advanced SEC column packed with sub-3 µm part...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Jelena Kova čić Daniela Amid žić Klarić Nik ša Turk Ana Mornar Source Type: research

Functionalization of selenium nanoparticles with olive polyphenols - impact on toxicity and antioxidative activity
Acta Pharm. 2023 Dec 26;73(4):617-631. doi: 10.2478/acph-2023-0036. Print 2023 Dec 1.ABSTRACTSelenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyviny...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Emerik Gali ć Kristina Radi ć Nikolina Golub Jakov Mlinar Dubravka Vitali Čepo Tomislav Vinkovi ć Source Type: research

Beliefs about medicines' association with endocrine therapy adherence in early breast cancer survivors in Croatia
Acta Pharm. 2023 Dec 26;73(4):673-689. doi: 10.2478/acph-2023-0043. Print 2023 Dec 1.ABSTRACTThis observational, cross-sectional study conducted at the University Hospital Centre Zagreb (UHC Zagreb) aimed to explore patients' beliefs about adjuvant endocrine therapy (AET) as well as their association with non-adherence and sociodemographic and clinical factors. Out of 420 early breast cancer (BC) patients included in the study, 79.5 % perceived AET necessary and important for their health, as measured by the Belief About Medicines Questionnaire (BMQ), with the mean necessity score (20.4 ± 3.68) significantly higher than t...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Ana Dugonji ć Okroša Tajana Silovski Natalija Dedi ć Plavetić Hrvoje Silovski Ana Kova čić Iva Mucalo Source Type: research

Preparation of astaxanthin/zeaxanthin-loaded nanostructured lipid carriers for enhanced bioavailability: Characterization-, stability-and permeability study
This study provides useful data about the advantages of using NLC as a delivery system for ASTA and ZEA that might facilitate their applications in the food and pharmaceutical industry.PMID:38147480 | DOI:10.2478/acph-2023-0038 (Source: Acta Pharmaceutica)
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Kristina Radi ć Ana Isabel Barbosa Salette Reis Marijan Marijan Sofia Antunes Costa Lima Dubravka Vitali Čepo Source Type: research

Exploring adherence in patients with advanced breast cancer: focus on CDK4/6 inhibitors
This study enhances understanding of medication adherence in advanced breast cancer and identifies influential factors.PMID:38147481 | DOI:10.2478/acph-2023-0045 (Source: Acta Pharmaceutica)
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Matea Bakovi ć Martina Bago Lucija Beni ć Magdalena Krajinovi ć Tajana Silovski Natalija Dedi ć Plavetić Lu Turkovi ć Miranda Serti ć Maja Ortner Had žiabdić Source Type: research

Synthesis, antiproliferative and antiplasmodial evaluation of new chloroquine and mefloquine-based harmiquins
Acta Pharm. 2023 Dec 26;73(4):537-558. doi: 10.2478/acph-2023-0035. Print 2023 Dec 1.ABSTRACTHere we present the synthesis and evaluation of the biological activity of new hybrid compounds, ureido-type (UT) harmiquins, based on chloroquine (CQ) or mefloquine (MQ) scaffolds and β-carboline alkaloid harmine against cancer cell lines and Plasmodium falciparum. The hybrids were prepared from the corresponding amines by 1,1'-carbonyldiimidazole (CDI)-mediated synthesis. In vitro evaluation of the biological activity of the title compounds revealed two hit compounds. Testing of the antiproliferative activity of the new UT harmi...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Kristina Pavi ć Goran Poje Lais Pessanha De Carvalho Jana Held Zrinka Raji ć Source Type: research

Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells
Acta Pharm. 2023 Dec 26;73(4):601-616. doi: 10.2478/acph-2023-0046. Print 2023 Dec 1.ABSTRACTOlive leaves as a main byproduct of olive oil and fruit industry are a valuable source of phytochemicals such as polyphenols, with multiple biomedical effects. Apart from leaves, olive branches and stems make up a significant amount of olive waste. It is well known that the drying process and long-term storage affect the stability and concentration of polyphenols present in raw materials. For that matter, two different means of storing olive waste, at room temperature and +4 °C, were compared by determining the content of the poly...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Đani Benčić Monika Barbari ć Ana Mornar Daniela Amid žić Klarić Anamaria Brozovic Sanja Dabeli ć Mihaela Fadljevi ć Ana Karkovi ć Marković Source Type: research

The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations
In conclusion, DMSO had different effects on leukocyte subpopulations in cryopre-served PBSC samples. The type of donors and the length of storage before cryopreservation did not affect the post-thaw stability of cryopreserved PBSC samples.PMID:38147484 | DOI:10.2478/acph-2023-0037 (Source: Acta Pharmaceutica)
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Vladimira Rimac Ines Bojani ć Sanja Dabeli ć Branka Golubi ć Ćepulić Source Type: research

Effects of epidurally administered dexmedetomidine and dexamethasone on postoperative pain, analgesic requirements, inflammation, and oxidative stress in thoracic surgery
Acta Pharm. 2023 Dec 26;73(4):691-708. doi: 10.2478/acph-2023-0040. Print 2023 Dec 1.ABSTRACTThe aim of this study was to compare the effects of dexmedetomidine and dexamethasone as adjuvants to preoperative epidural administration of local anesthetic (ropivacaine) in thoracic surgery on the postoperative level of pain, use of analgesics, inflammation, and oxidative stress. The study enrolled 42 patients who underwent elective thoracic surgery in a one-year period at the University Hospital Dubrava (Zagreb, Croatia). Based on a computer-generated randomization list the patients were assigned to the dexmedetomidine (n = 18)...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Jasminka Per šec Andrej Šribar Monika Ili ć Ivan Mami ć Domagoj Kifer Ana-Marija Domijan Željan Maleš Petra Tur čić Source Type: research

Azithromycin-loaded liposomal hydrogel: a step forward for enhanced treatment of MRSA-related skin infections
Acta Pharm. 2023 Dec 26;73(4):559-579. doi: 10.2478/acph-2023-0042. Print 2023 Dec 1.ABSTRACTAzithromycin (AZT) encapsulated into various types of liposomes (AZT-liposomes) displayed pronounced in vitro activity against methicillin-resistant Staphylococcus aureus (MRSA) (1). The present study represents a follow-up to this previous work, attempting to further explore the anti-MRSA potential of AZT-liposomes when incorporated into chitosan hydrogel (CHG). Incorporation of AZT-liposomes into CHG (liposomal CHGs) was intended to ensure proper viscosity and texture properties of the formulation, modification of antibiotic rele...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Zora Rukavina May Wenche J øraholmen Dunja Bo žić Ivana Frankol Petra Golja Ga šparović Nata ša Škalko-Basnet Maja Šegvić Klarić Željka Vanić Source Type: research

Simultaneous determination of macrolides in water samples by solid-phase extraction and capillary electrophoresis
Acta Pharm. 2023 Dec 26;73(4):515-535. doi: 10.2478/acph-2023-0041. Print 2023 Dec 1.ABSTRACTSolid-phase extraction (SPE) coupled with capillary electrophoresis (CE) for the determination of macrolide antibiotics (azithromycin, clarithromycin, roxithromycin, tylosin) and tiamulin in water samples was described in this article. These compounds were extracted with different types of sorbents ( Oasis HLB, C18, C8, SDB, and Strata-X) and different masses of sorbents (60 mg, 200 mg, and 500 mg) using different organic solvents (methanol, ethanol, and acetonitrile) and different pH values of water samples (pH 7.00, 8.00, and 9.0...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Dragana Mutavd žić Pavlović Sandra Babi ć Mirta Čizmić Miranda Serti ć Tea Pinu šić Source Type: research

Prescribers' approval rate of pharmacist-initiated interventions to optimise patients' clinical status of hypertension in the ambulatory care setting
Acta Pharm. 2023 Dec 26;73(4):723-734. doi: 10.2478/acph-2023-0047. Print 2023 Dec 1.ABSTRACTThis perspective, pre- and post-intervention study with a one-year follow-up primarily aimed to ascertain prescribers' approval rate of pharmacists' interventions and clinical status of hypertension following comprehensive medication management (CMM) intervention in the ambulatory care clinic. Between January 2018 and January 2022 overall 100 patients with hypertension and other comorbidities were referred to the CMM services at the Health Centre Zagreb - Centar (HCZC). Out of 275 interventions directed to prescribers, 73.1 % of in...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Andrea Brajkovi ć Lucija Ana Bi ćanić Helena Orehova čki Ingrid Prka čin Djenane Ramalho De Oliveira Iva Mucalo Source Type: research