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RTCB deficiency triggers colitis in mice by influencing the NF- κB and Wnt/β-catenin signaling pathways
In this study, we employ a conditional knockout mouse model to investigate the effects of RTCB depletion on the colon and the potential molecular mechanisms. We assess the efficiency and phenotype of Rtcb knockout using PCR, western blot analysis, histological staining, and immunohistochemistry. Compared with the control mice, the Rtcb-knockout mice exhibit compromised colonic barrier integrity and prominent inflammatory cell infiltration. In the colonic tissues of Rtcb-knockout mice, the protein levels of TNF-α, IL-8, and p-p65 are increased, whereas the levels of IKKβ and IκBα are decreased. Moreover, the level of GS...
Source: Acta Biochimica et Biophysica Sinica - March 1, 2024 Category: Biochemistry Authors: Peiyan Liu Ruitao Zhang Xiaotong Song Xiaohua Tian Yichao Guan Licheng Li Mei He Chengqiang He Naizheng Ding Source Type: research

Identification of neutrophil extracellular trap-driven gastric cancer heterogeneity and C5AR1 as a therapeutic target
Acta Biochim Biophys Sin (Shanghai). 2024 Mar 1. doi: 10.3724/abbs.2023290. Online ahead of print.ABSTRACTNeutrophil extracellular traps (NETs) are implicated in gastric cancer (GC) growth, metastatic dissemination, cancer-associated thrombosis, etc. This work is conducted to elucidate the heterogeneity of NETs in GC. The transcriptome heterogeneity of NETs is investigated in TCGA-STAD via a consensus clustering algorithm, with subsequent external verification in the GSE88433 and GSE88437 cohorts. Clinical and molecular traits, the immune microenvironment, and drug response are characterized in the identified NET-based clu...
Source: Acta Biochimica et Biophysica Sinica - March 1, 2024 Category: Biochemistry Authors: Jing Zhao Xiangyu Li Liming Li Beibei Chen Weifeng Xu Yunduan He Xiaobing Chen Source Type: research

TRMT13 inhibits the growth of papillary thyroid cancer by targeting ANAPC4
Acta Biochim Biophys Sin (Shanghai). 2024 Mar 1. doi: 10.3724/abbs.2024010. Online ahead of print.ABSTRACTThe recently discovered gene TRMT13 encodes a type of RNA methylase and is a member of the CCDC family (also called CCDC76). Here, we delineate its role in papillary thyroid cancer (PTC). Bioinformatics analysis shows significant TRMT13 and ANAPC4 downregulation in PTC and reveals that the expression levels of both genes are linearly correlated. Subsequent analyses confirm that both TRMT13 and ANAPC4 expressions are downregulated in PTC tissues and that this change in expression has a significant impact on cancer diagn...
Source: Acta Biochimica et Biophysica Sinica - March 1, 2024 Category: Biochemistry Authors: Lianyong Liu Yan Wang Mei Zou Shiwei Chen Fengying Wu Xiangqi Li Source Type: research

Rutaecarpine ameliorates imiquimod-induced psoriasis-like dermatitis in mice associated with alterations in the gut microbiota
In conclusion, the gut microbiota might be a therapeutic target or biomarker for psoriasis that aids in clinical diagnosis and therapy.PMID:38419497 | DOI:10.3724/abbs.2024018 (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - February 29, 2024 Category: Biochemistry Authors: Yongjian Li Zhengping Tan Wencan Li Zongxuan Li Guiying Zhang Source Type: research

A subunit vaccine based on Brucella melitensis rBP26 induces Th1 immune responses and M1 macrophage activation
Acta Biochim Biophys Sin (Shanghai). 2024 Feb 29. doi: 10.3724/abbs.2024023. Online ahead of print.ABSTRACTBrucellosis is a global zoonotic infection caused by Brucella bacteria, which poses a significant burden on society. While transmission prevention is currently the most effective method, the absence of a licenced vaccine for humans necessitates the urgent development of a safe and effective vaccine. Recombinant protein-based subunit vaccines are considered promising options, and in this study, the Brucella BP26 protein is expressed using prokaryotic expression systems. The immune responses are evaluated using the well...
Source: Acta Biochimica et Biophysica Sinica - February 29, 2024 Category: Biochemistry Authors: Jia Wen Zihua Li Yongxue Lv Shuqin Ding Yazhou Zhu Jihui Yang Jing Tang Mingxing Zhu Yinqi Zhao Wei Zhao Source Type: research

HSPA8-mediated stability of the CLPP protein regulates mitochondrial autophagy in cisplatin-resistant ovarian cancer cells
In conclusion, CLPP increases DDP resistance in ovarian cancer by inhibiting mitophagy and promoting cellular stress. Meanwhile, HSPA8 promotes the degradation of CLPP protein by regulating its stability.PMID:38419499 | DOI:10.3724/abbs.2023246 (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - February 29, 2024 Category: Biochemistry Authors: Xinxin Kou Xiaoxia Yang Zheng Zhao Lei Li Source Type: research

 RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs
In this study, we verify the crucial role of H3K27me3 in RASGRP1 regulation. The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells (PSCs). The results of qPCR, western blot analysis, EdU staining, CCK-8 assay and immunofluorescence staining show that overexpression of RASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models, while knockdown of RASGRP1 leads to the opposite results. These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mi...
Source: Acta Biochimica et Biophysica Sinica - February 29, 2024 Category: Biochemistry Authors: Liyao Xiao Jiaxin Qiao Yiyang Huang Baohua Tan Linjun Hong Zicong Li Gengyuan Cai Zhenfang Wu Enqin Zheng Shanshan Wang Ting Gu Source Type: research

Targeted disruption of the BCR-ABL fusion gene by Cas9/dual-sgRNA inhibits proliferation and induces apoptosis in chronic myeloid leukemia cells
This study aims to explore the efficiency of novel CRISPR-associated protein 9 (Cas9)/dual-single guide RNA (sgRNA)-mediated disruption of the BCR-ABL fusion gene by targeting BCR and c-ABL introns. A co-expression vector for Cas9 green fluorescent protein (GFP)/dual-BA-sgRNA targeting BCR and c-ABL introns is constructed to produce lentivirus to affect BCR-ABL expression in CML cells. The effects of dual-sgRNA virus-mediated disruption of BCR-ABL are analyzed via the use of a genomic sequence and at the protein expression level. Cell proliferation, cell clonogenic ability, and cell apoptosis are assessed after dual sgRNA ...
Source: Acta Biochimica et Biophysica Sinica - February 28, 2024 Category: Biochemistry Authors: Jianling Zeng Xinquan Liang Lili Duan Fenghua Tan Liujie Chen Jiayao Qu Jia Li Kai Li Dixian Luo Zheng Hu Source Type: research

miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2
In this study, we explore the effect and mechanisms of miR-29b-1-5p on sepsis-induced myocardial damage. Sepsis-associated Gene Expression Omnibus (GEO) datasets (GSE72380 and GSE29914) are examined for differential miRNAs. The mouse sepsis-induced cardiac injury was established by Lipopolysaccharide (LPS) or cecal ligation and puncture (CLP). LPS-treated HL-1 mouse cardiomyocytes simulate myocardial injury in vitro. miR-29b-1-5p is co-upregulated in both datasets and in cardiac tissue from sepsis mouse and HL-1 cell models. miR-29b-1-5p expression downregulation was achieved by antagomir transduction and confirmed by real...
Source: Acta Biochimica et Biophysica Sinica - February 28, 2024 Category: Biochemistry Authors: Yaqing Jiang Junmei Xu Hua Zeng Zhaojing Lin Qiong Yi Jiali Guo Feng Xiao Source Type: research

AHR signaling pathway mediates mitochondrial oxidative phosphorylation which leads to cytarabine resistance
In this study, RNA-Seq data from AML patients (bone marrow samples from 173 newly diagnosed AML patients) obtained from the TCGA database, and normal human RNA-Seq data (bone marrow samples from 70 healthy individuals) obtained from the GTEX database are downloaded for external validation and complementarity. The data analysis reveals that the AHR signaling pathway is activated in AML patients. Furthermore, there is a correlation between the expressions of AHR and mitochondrial oxidative phosphorylation genes. In vitro experiments show that enhancing AHR expression in AML cells increases mitochondrial oxidative phosphoryla...
Source: Acta Biochimica et Biophysica Sinica - February 26, 2024 Category: Biochemistry Authors: Yan Jia Xiyu Li Lulu Chen Ling Li Suzhen Zhang Wenhui Huang Hao Zhang Source Type: research

Agrimol B alleviates cisplatin-induced acute kidney injury by activating the Sirt1/Nrf2 signaling pathway in mice
Acta Biochim Biophys Sin (Shanghai). 2024 Feb 26. doi: 10.3724/abbs.2023285. Online ahead of print.ABSTRACTCisplatin (CDDP) is a widely used chemotherapeutic agent that has remarkable antineoplastic effects. However, CDDP can cause severe acute kidney injury (AKI), which limits its clinical application. Agrimol B is the main active ingredient found in Agrimonia pilosa Ledeb and has a variety of pharmacological activities. The effect of agrimol B on CDDP-induced renal toxicity has not been determined. To investigate whether agrimol B has a protective effect against CDDP-induced AKI, we first identify Sirtuin 1 (Sirt1) as a ...
Source: Acta Biochimica et Biophysica Sinica - February 26, 2024 Category: Biochemistry Authors: Jiarui Tang Longhui Li Zhijian Chen Cuiting Liao Kai Hu Yongqiang Yang Jiayi Huang Li Tang Li Zhang Longjiang Li Source Type: research