Acta Biochimica et Biophysica Sinica This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

p32/OPA1 axis-mediated mitochondrial dynamics contributes to cisplatin resistance in non-small cell lung cancer
This study aims to investigate the role of the p32/OPA1 axis in cisplatin resistance in NSCLC and its underlying mechanism. The levels of p32 protein and mitochondrial fusion protein OPA1 are higher in cisplatin-resistant A549/DDP cells than in cisplatin-sensitive A549 cells, which facilitates mitochondrial fusion in A549/DDP cells. In addition, the expression of p32 and OPA1 protein is also upregulated in A549 cells during the development of cisplatin resistance. Moreover, p32 knockdown effectively downregulates the expression of OPA1, stimulates mitochondrial fission, decreases ATP generation and sensitizes A549/DDP cell...
Source: Acta Biochimica et Biophysica Sinica - December 28, 2023 Category: Biochemistry Authors: Chun-Xia Yu Zhe-Qing Peng Tao Wang Xin-Hui Qu Ping Yang Shao-Rong Huang Li-Ping Jiang Fang-Fang Tou Xiao-Jian Han Source Type: research

Demethylase FTO inhibits the occurrence and development of triple-negative breast cancer by blocking m 6 A-dependent miR-17-5p maturation-induced ZBTB4 depletion
In this study, we aim to investigate the role and molecular mechanisms of the demethylase FTO (fat mass and obesity-associated protein) in TNBC. Through analysis of public databases, we identify that FTO may regulate the maturation of miR-17-5p and subsequently influence the expression of zinc finger and BTB domain-containing protein 4 (ZBTB4), thereby affecting the occurrence and progression of TNBC. We screen for relevant miRNAs and mRNAs from the GEO and TCGA databases and find that the FTO gene may play a crucial role in TNBC. In vitro cell experiments demonstrate that overexpression of FTO can suppress the proliferati...
Source: Acta Biochimica et Biophysica Sinica - December 28, 2023 Category: Biochemistry Authors: Jingyi Ni Xiaoyun Lu Xiangxiang Gao Conghui Jin Junfeng Mao Source Type: research

Immunoprotective effect and mechanism of rEg.P29 against CD4 + T cell-deficient mice with Echinococcus multilocularis infection
In this study, CD4 + T cell-deficient mice are transferred with spleen CD4 + T cells isolated from wild-type mice and subjected to rEg.P29 immunization, and then these immunized mice are infected with E. multilocularis. The cyst inhibition rate is calculated by weighing the body and cyst weights. The level of antibody is detected by ELISA. Flow cytometry is used to detect the level of IFN-γ production by CD4 + T and CD8 + T cells. The cytokines in culture supernatant are detected by ELISA. The expressions of CD44 and CD62L on memory T cells are determined by flow cytometry. The results show the cyst inhibition rate is 41....
Source: Acta Biochimica et Biophysica Sinica - December 28, 2023 Category: Biochemistry Authors: Ming Li Yazhou Zhu Zihua Li Jiahui Song Wei Zhao Source Type: research

RIP1/3-dependent programmed necrosis induces intestinal injury in septic rats
Acta Biochim Biophys Sin (Shanghai). 2023 Dec 28. doi: 10.3724/abbs.2023248. Online ahead of print.ABSTRACTThe regulation of various types of cell death may help to restore the normal physiological function of cells and play a protective role in sepsis. In the current study, we explore the role of programmed cell necrosis in sepsis and the underlying mechanisms. The septic rat model is established by Cecal-ligation and perforation (CLP), and the in vitro model is established by LPS in IEC-6 cells. Our results demonstrate that receptor-interacting protein 1 (RIP1) is significantly upregulated in the ileum of septic rats and...
Source: Acta Biochimica et Biophysica Sinica - December 28, 2023 Category: Biochemistry Authors: Siting Ye Xinxin Qi Yuxiao Liu Liangming Zhuang Zhongmin Gu Source Type: research

A network pharmacology- and transcriptomics-based investigation reveals an inhibitory role of β-sitosterol in glioma via the EGFR/MAPK signaling pathway
Acta Biochim Biophys Sin (Shanghai). 2023 Dec 25. doi: 10.3724/abbs.2023251. Online ahead of print.ABSTRACTGlioma is characterized by rapid cell proliferation, aggressive invasion, altered apoptosis and a poor prognosis. β-Sitosterol, a kind of phytosterol, has been shown to possess anticancer activities. Our current study aims to investigate the effects of β-sitosterol on gliomas and reveal the underlying mechanisms. Our results show that β-sitosterol effectively inhibits the growth of U87 cells by inhibiting proliferation and inducing G2/M phase arrest and apoptosis. In addition, β-sitosterol inhibits migration by do...
Source: Acta Biochimica et Biophysica Sinica - December 25, 2023 Category: Biochemistry Authors: Yufang Xie Zhijian Chen Shuang Li Meijuan Yan Wenjun He Li Li Junqiang Si Yan Wang Xinzhi Li Ketao Ma Source Type: research

Upregulation of E-cadherin by the combination of methionine restriction and HDAC2 intervention for inhibiting gastric carcinoma metastasis
In this study, we aim to explore the effect and related mechanisms of methionine restriction (MR) on gastric carcinoma metastasis. In the MR cell model, gastric carcinoma cells are cultured in the MR medium, and in the animal model, BALB/c nude rodents are administered with a methionine-free diet after receiving injections of MKN45 cells into the caudal vein. Transwell assay is used to detect cell invasion and migration. Chromatin immunoprecipitation is performed to investigate the levels of H3K9me2, H3K27Ac, and H3K27me3 in the E-cadherin promoter. The results show that MR inhibits gastric carcinoma cell migration, invasi...
Source: Acta Biochimica et Biophysica Sinica - December 25, 2023 Category: Biochemistry Authors: Yifan Li Chenxi Liu Lin Xin Chuan Liu Jiaqing Cao Zhenqi Yue Jie Sheng Yiwu Yuan Qi Zhou Zhiyang Liu Source Type: research