Expression of the immune regulation antigen CD70 in osteosarcoma

In this study, expression of CD70 and CD27 was analyzed in osteosarcoma cell lines and tumor specimens.CD70 protein was expressed on most osteosarcoma cell lines (5/7) and patient-derived primary osteosarcoma cultures (4/6) as measured by flow cytometry. In contrast, CD70 was detected on few Ewing sarcoma cell lines (5/15) and was virtually absent from neuroblastoma (1/7) and rhabdomyosarcoma cell lines (0/5). CD70+ primary cultures were derived from CD70+ osteosarcoma lesions. CD70 expression in osteosarcoma cryosections was heterogeneous, restricted to tumor cells and not attributed to infiltrating CD3+ T cells as assessed by immunohistochemistry/immunofluorescence. CD70 was detected in primary (1/5) but also recurrent (2/4) and metastatic (1/3) tumors. CD27, the receptor for CD70, was neither detected on tumor cells nor on T cells in CD70+ or CD70− tumors, suggesting that CD70 on tumor cells is not involved in CD27-dependent tumor-immune cell interactions in osteosarcoma. CD70 gene expression in diagnostic biopsies of osteosarcoma patients did not correlate with the occurrence of metastasis and survival (n = 70).Our data illustrate that CD70 is expressed in a subset of osteosarcoma patients. In patients with CD70+ tumors, CD70 may represent a novel candidate for antibody-based targeted immunotherapy.
Source: Cancer Cell International - Category: Cancer & Oncology Authors: Source Type: research

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Source: Molecular and Cellular Endocrinology - Category: Endocrinology Source Type: research
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Source: Trends in Pharmacological Sciences - Category: Drugs & Pharmacology Source Type: research
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Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
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Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
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Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
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Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
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Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
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