Computational study of ß- N -acetylhexosaminidase from Talaromyces flavus , a glycosidase with high substrate flexibility

Conclusions: The main variable regions in ?-N-acetylhexosaminidases determining difference in modified substrate affinity are located close to the active site entrance and engage two loops. Differences in primary sequence and the spatial arrangement of these loops and their interplay with active site amino acids, reflected by interaction energies and dynamics, account for the different catalytic activity and substrate specificity of the various fungal and bacterial ?-N-acetylhexosaminidases.
Source: BMC Bioinformatics - Category: Bioinformatics Authors: Source Type: research

Related Links:

Eur J Med Chem. 2022 Jan 12;230:114117. doi: 10.1016/j.ejmech.2022.114117. Online ahead of print.ABSTRACTThe mangrove forests are a complex ecosystem, and the microbial communities in mangrove sediments play a critical role in the biogeochemical cycles of mangrove ecosystems. Mangrove sediments-derived microbes (MSM), as a rich reservoir of natural product diversity, could be utilized in the exploration of new antibiotics or drugs. To understand the structural diversity and bioactivities of the metabolites of MSM, this review for the first time provides a comprehensive overview of 519 natural products isolated from MSM wit...
Source: European Journal of Medicinal Chemistry - Category: Chemistry Authors: Source Type: research
Environ Res. 2022 Jan 18:112770. doi: 10.1016/j.envres.2022.112770. Online ahead of print.ABSTRACTZerovalent Iron Nanoparticles (MC-ZVI NPs) were synthesized from Musa coocinea peel extract as reducing and stabilizing agent using a novel synthesis technique. The synthesis of MC-ZVI NPs was confirmed using UV-Vis spectroscopy showing a absorption maximum at 341 nm. Further the chemical and structural characterization of MC-ZVI NPs was performed using Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering technique (DLS). FTIR analysis revealed the presence of phytoch...
Source: Environmental Research - Category: Environmental Health Authors: Source Type: research
Food Chem Toxicol. 2022 Jan 18:112823. doi: 10.1016/j.fct.2022.112823. Online ahead of print.ABSTRACTAflatoxins are produced as secondary metabolites by the toxigenic Aspergillus fungi. Among the aflatoxins, aflatoxin B1 (AFB1) is a common contaminant of global concern in human and animal food products. Prolonged exposure to AFB1 may provoke hepatocyte pyroptosis and oxidative stress, which leads to liver damage. Dietary polyphenols could protect the liver from a wide range of toxins. Curcumin, a polyphenolic substance derived from turmeric, is rich in pharmacological activity. The aim of this study was to systematically i...
Source: Food and Chemical Toxicology - Category: Food Science Authors: Source Type: research
With population genetic evidence of recombination ongoing in the natural Aspergillus fumigatus population and a sexual cycle demonstrated in the laboratory the question remained what the natural niche for A. fumigatus sex is. Composting plant-waste material is a known substrate of A. fumigatus to thrive and withstand temperatures even up to 70°C. Previous studies have shown indirect evidence for sexual reproduction in these heaps but never directly demonstrated the sexual structures due to technical limitations. Here, we show that flower bulb waste material from stockpiles undergoing composting can provide the conditio...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
CONCLUSION: Ocular trauma is a major risk factor for IK in Vietnam, which is diagnosed in almost 400 patients each year at VNEH. Given this, and as approximately one quarter of the eyes that develop IK require corneal transplantation or evisceration, greater emphasis should be placed on the development of prevention and treatment programs for IK in Vietnam.PMID:35047367 | PMC:PMC8720338 | DOI:10.18240/ijo.2022.01.19
Source: Herpes - Category: Infectious Diseases Authors: Source Type: research
Updated Date: Thu, 20 Jan 2022 00:00:00 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
Better treatment for Candida auris, Aspergillus and other dangerous fungal pathogens is slow to come, even as rates of drug resistance rise. New therapies are in the pipeline, and hospital practices can help.Knowable Magazine
Source: Medscape Dermatology Headlines - Category: Dermatology Tags: Infectious Diseases News Source Type: news
Natural yeast with superior fermentative traits can serve as a platform for the development of recombinant strains that can be used to improve the sustainability of bioethanol production from starch. This process will benefit from a consolidated bioprocessing (CBP) approach where an engineered strain producing amylases directly converts starch into ethanol. The yeast Saccharomyces cerevisiae L20, previously selected as outperforming the benchmark yeast Ethanol Red, was here subjected to a comparative genomic investigation using a dataset of industrial S. cerevisiae strains. Along with Ethanol Red, strain L20 was then engin...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Chronic granulomatous disease (CGD) is a rare inherited primary immunodeficiency syndrome, manifested as recurrent infections and inflammatory complications. Although prophylactic treatment with antibiotics an...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Case report Source Type: research
In conclusion, efficient genetic manipulation systems along with a variety of functional mutants were developed in this study, which would significantly expedite both theoretical and applied researches in not only A. terreus but also other marine-derived filamentous fungi.PMID:35043238 | DOI:10.1007/s00294-021-01218-8
Source: Current Genetics - Category: Genetics & Stem Cells Authors: Source Type: research
More News: Aspergillus | Bioinformatics | Study