Subcellular Compartmentalization and Trafficking of the Biosynthetic Machinery for Fungal Melanin

Publication date: Available online 10 March 2016 Source:Cell Reports Author(s): Srijana Upadhyay, Xinping Xu, David Lowry, Jennifer C. Jackson, Robert W. Roberson, Xiaorong Lin Protection by melanin depends on its subcellular location. Although most filamentous fungi synthesize melanin via a polyketide synthase pathway, where and how melanin biosynthesis occurs and how it is deposited as extracellular granules remain elusive. Using a forward genetic screen in the pathogen Aspergillus fumigatus, we find that mutations in an endosomal sorting nexin abolish melanin cell-wall deposition. We find that all enzymes involved in the early steps of melanin biosynthesis are recruited to endosomes through a non-conventional secretory pathway. In contrast, late melanin enzymes accumulate in the cell wall. Such subcellular compartmentalization of the melanin biosynthetic machinery occurs in both A. fumigatus and A. nidulans. Thus, fungal melanin biosynthesis appears to be initiated in endosomes with exocytosis leading to melanin extracellular deposition, much like the synthesis and trafficking of mammalian melanin in endosomally derived melanosomes. Graphical abstract Teaser Upadhyay et al. discovered that fungal melanin biosynthetic machinery is recruited to endosomes, although it is composed of largely atypical secretory proteins. Compartmentalization and trafficking through the endosomal system might be important cellular principles governing fungal...
Source: Cell Reports - Category: Cytology Source Type: research

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Source: Food Control - Category: Food Science Source Type: research
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Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Medical Imagery Source Type: research
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Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research
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Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
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Source: Fungal Genetics and Biology - Category: Biology Authors: Tags: Fungal Genet Biol Source Type: research
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