Protonation state of F420H2 in the prodrug-activating deazaflavin dependent nitroreductase (Ddn) from Mycobacterium tuberculosis

Mol. BioSyst., 2016, Accepted ManuscriptDOI: 10.1039/C6MB00033A, CommunicationElaaf Mohamed, Hafna Ahmed, Sundaram Arulmozhiraja, Ching Yeh Lin, Matthew C Taylor, Elmars Krausz, Colin Jackson, Michelle CooteThe protonation state of the deazaflavin dependent nitroreductase (Ddn) enzyme bound cofactor F420 was investigated using UV-visble spectroscopy and computational simulations. The reduced cofactor F420H2 was determined to be present...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Molecular Biosystems - Category: Molecular Biology Authors: Source Type: research

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Publication date: Available online 20 February 2020Source: Progress in Biophysics and Molecular BiologyAuthor(s): Gerhard GrüberAbstractMycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), kills more people than any other bacterium. TB control is threatened by the continued spread of drug resistance; multi-drug and extensively drug resistant Mtb require longer, more costly, treatment with multiple drugs causing worse side effects and have a lower likelihood of treatment success. The urgent need for better treatment options for drug resistant Mtb has led the World Health Organization to prioritiz...
Source: Progress in Biophysics and Molecular Biology - Category: Molecular Biology Source Type: research
Publication date: Available online 20 February 2020Source: Journal of Molecular LiquidsAuthor(s): Bernice Rodrigues, Pravin ShendeAbstractThe aim of the research study was to explore the applications of dendrimer complexed with copper in the form of nanoclusters with drug isoniazid by acting on M. tuberculosis H37Ra (ATCC 25177) cells. This approach resulted to minimize dosage regimen, reduce toxicity and provide site-specific action through pulmonary delivery. Copper nanoclusters were prepared using template synthesis approach that includes preparation of G 4 dendrimers using Michael addition and amidation reactions and a...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
ConclusionA comparative high-resolution analysis of B*35Px or B*35Py alleles among healthy controls and HIV+ individuals showed an overrepresentation of HLA-B*35Px alleles in the healthy Indian gene pool (58%) and in the HIV+ cohort (70%). The most common B*35 subtype found among the HIV infected subjects was B*3503/55 (20%; Px group), followed by B*3501 (10%; Py group). Our study concludes that the Indian gene pool possesses higher frequency of B*35Px alleles.
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
Publication date: March 2020Source: The Lancet Global Health, Volume 8, Issue 3Author(s): Charbel El Bcheraoui, Honoré Mimche, Yodé Miangotar, Varsha Sarah Krish, Faye Ziegeweid, Kris J Krohn, Martin Herbas Ekat, Jobert Richie Nansseu, Zacharie Tsala Dimbuene, Helen Elizabeth Olsen, Roger C K Tine, Christopher M Odell, Christopher E Troeger, Nicholas J Kassebaum, Tamer Farag, Simon I Hay, Ali H MokdadSummaryBackgroundPeer-reviewed literature on health is almost exclusively published in English, limiting the uptake of research for decision making in francophone African countries. We used results from the Globa...
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
The CDC recently updated guidelines for the treatment of latent tuberculosis infection, the first comprehensive guidelines on LTBI issued since 2000.
Source: AAFP News - Category: Primary Care Source Type: news
Publication date: Available online 18 February 2020Source: Progress in Biophysics and Molecular BiologyAuthor(s): Bei Shi Lee, Ekaterina Sviriaeva, Kevin PetheAbstractMycobacterium tuberculosis strictly depends on oxygen to multiply, and the terminal oxidases are a vital part of the oxidative phosphorylation pathway. The bacterium possesses two aerobic respiratory branches: a cytochrome bcc-aa3 and a bacteria-specific cytochrome bd oxidase. The identification of small-molecule inhibitors of the cytochrome bcc-aa3 under numerous experimental conditions reflects the essentiality of the pathway for the optimum growth of M. tu...
Source: Progress in Biophysics and Molecular Biology - Category: Molecular Biology Source Type: research
Conclusions Histology is proved to be an accurate diagnostic tool in TS. Suppurative forms of TS without granuloma are rare and represent the main cause of false-negative histology. Suggestive histology of TS does not rule out fungal and brucellar spondylodiscitis. Caseous necrosis is not pathognomonic of tuberculosis. Fungal infection can also exhibit such type of necrosis.
Source: JCR: Journal of Clinical Rheumatology - Category: Rheumatology Tags: Original Articles Source Type: research
ConclusionHS016 resembled adalimumab in efficacy and safety over the 24-week treatment period.Trial registration numberChiCTR1900022520.
Source: BioDrugs - Category: Drugs & Pharmacology Source Type: research
und Most of the targeted discoveries in tuberculosis research have covered previously explored chemical structures but neglected physiochemical properties. Until now, no efficient prediction tools have been developed to discriminate the novelty of screened compounds at early stages. To overcome this deficit, a drastic novel approach must include physicochemical properties filters provided by Chemical Global Positioning System-Natural Product analysis (ChemGPS-NP). Three different screening schemes GSK, GVKBio, and NIAID provided 776, 2880, and 3779 compounds respectively and were evaluated based on their physicochemica...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Tuberculosis (TB) is an infectious disease of extremely high epidemiological burden worldwide that is easily acquired through the inhalation of infected respiratory droplets. The complex pathogenesis of this infection spans from subjects never developing this disease despite intense exposure, to others in which immune containment fails catastrophically and severe or disseminated forms of disease ensue. In recent decades, microRNAs (miRNAs) have gained increasing attention due to their role as gene silencers and because of their altered expression in diverse human diseases, including some infections. Recent research regardi...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Minireviews Source Type: research
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