Molecular Characterization of Plasmids Encoding CTX-M β-Lactamases and their Associated Addiction Systems Circulating among Escherichia coli from Retail Chickens, Chicken Farms, and Slaughterhouses in Korea.

Molecular Characterization of Plasmids Encoding CTX-M β-Lactamases and their Associated Addiction Systems Circulating among Escherichia coli from Retail Chickens, Chicken Farms, and Slaughterhouses in Korea. J Microbiol Biotechnol. 2015 Nov 12; Authors: Jo SJ, Woo GJ Abstract Extended-spectrum β-lactamases (ESBLs), particularly those of the CTX-M types, are the predominant resistance determinants of Escherichia coli that are rapidly spreading worldwide. To determine CTX-M-types, E. coli isolates collected from retail chickens and environmental samples from chicken farms and slaughterhouses in Korea. Fifteen strains harboring blaCTX-M genes from 358 E. coli isolates. The most common CTX-M type was 8 of CTX-M-15, followed by 6 of CTX-M-1 and 1 of CTX-M-14. The blaCTX-M genes were identified in the isolates from retail chickens (n = 9), followed by feces, water pipes, floors and walls. Conjugations confirmed the transferability of the plasmids carrying blaCTX-M genes to the recipient E. coli J53 strain. Furthermore, association with eight addiction systems was determined, with the dominant systems identified as ccdAB, vagCD, and pndAC, both in donor strains and transconjugants. The clonal relationship between the two strains carrying blaCTX-M genes indicates that E. coli may transmit from the farm to retail chickens, suggesting a possible public health risk. Our findings demonstrate that the detection of CTX-M types in E. coli isolates is importan...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research

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