Genome Engineering with Synthetic Copper Nucleases

SynlettDOI: 10.1055/s-0035-1560709Oxidative chemical nucleases represent an important class of synthetic compound that mediate nucleic acid recognition and strand scission. These agents have found utility as footprinting probes to map drug–DNA and protein–DNA interactions and also as clinical cytotoxins of human cancer. Herein, we highlight a recent contribution from our group on the application of the copper(II) 1,10-phenanthroline (OP) complex Cu(OP)2 as a novel DNA-shuffling agent capable of generating highly diverse mutant protein antibody libraries. By employing a recombinant antibody gene fragment specific for prostate-specific antigen (PSA), we compared variation and PSA-binding specificity within Taq DNA polymerase I reconstructed Cu(OP)2 oxidative digest fragments with hydrolytic restriction digests generated by DNase I. Results show the Cu(OP)2 mutant library contained highly PSA-specific binding antibodies thus indicating that oxidative chemical nucleases may be applied to generate novel antibody clones not accessible through the use of the traditional DNA-shuffling enzyme DNase I.[...]© Georg Thieme Verlag Stuttgart · New YorkArticle in Thieme eJournals:Table of contents  |  Abstract  |  open access Full text
Source: Synlett - Category: Chemistry Authors: Tags: synpacts Source Type: research

Related Links:

Authors: Pucci C, Martinelli C, Ciofani G Abstract Every year, cancer is responsible for millions of deaths worldwide and, even though much progress has been achieved in medicine, there are still many issues that must be addressed in order to improve cancer therapy. For this reason, oncological research is putting a lot of effort towards finding new and efficient therapies which can alleviate critical side effects caused by conventional treatments. Different technologies are currently under evaluation in clinical trials or have been already introduced into clinical practice. While nanomedicine is contributing to th...
Source: Ecancermedicalscience - Category: Cancer & Oncology Tags: Ecancermedicalscience Source Type: research
Authors: Li X, Wu N, Ji H, Huang Y, Hu H, Li J, Mi S, Duan S, Chen X Abstract The AGTR1 gene encodes angiotensin II receptor type 1, which is involved in cardiovascular diseases such as coronary heart disease (CHD). In the current study, we analyzed AGTR1 methylation level in a Han Chinese population by SYBR green-based quantitative methylation-specific PCR (qMSP). We collected blood samples from 761 CHD patients and 398 non-CHD controls at the Ningbo First Hospital. A data mining analysis was also performed to explore the association between AGTR1 methylation and AGTR1 gene expression, using datasets from the cBio...
Source: Bosnian Journal of Basic Medical Sciences - Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research
This study provides valuable experimental evidence for clinical MM therapy. PMID: 31538911 [PubMed - as supplied by publisher]
Source: Bosnian Journal of Basic Medical Sciences - Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research
Many radiologists are concerned that mammography information provided by referring physicians is confusing for patients, and they want to be more involved in screening-related discussions, according to a  studyrecently published in theJournal of Breast Imaging. The group of researchers led by Shadi Aminololama-Shakeri, MD,Chief of Breast Imaging Section at theUniversity of California Davis in Sacramento, California found thatthere is a wide variety of breast cancer screening guidelines across professional societies, and the lack of uniformity makes understanding the purpose of mammography confusing and frustratin...
Source: radRounds - Category: Radiology Authors: Source Type: blogs
ConclusionMR Esophagography might become a non-invasive, non-irradiating technique of choice following diagnostic esophagogastroduodenoscopy for the assessment of esophageal diseases.
Source: European Journal of Radiology - Category: Radiology Source Type: research
ConclusionIn breast cancer, D and f values show significant differences according to TSR, and D value is lower in collagen dominant type than in fibroblast dominant or lymphocyte dominant types.
Source: European Journal of Radiology - Category: Radiology Source Type: research
Abstract Traditional tobacco products have well-known systemic and local oral effects, including inflammation, vasoconstriction, delayed wound healing, and increased severity of periodontal disease. Specifically in the oral cavity and the lung, cigarette smoking produces cancer, increased infectivity, acute and chronic inflammation, changes in gene expression in epithelial lining cells, and microbiome changes. In recent years, cigarette smoking has greatly decreased in the United States, but the use of new tobacco products has gained tremendous popularity. Without significant knowledge of the oral sequelae of prod...
Source: Adv Dent Res - Category: Dentistry Authors: Tags: Adv Dent Res Source Type: research
Abstract Electronic nicotine delivery systems (ENDS) are devices that contain a power source, a heating element, and a tank or cartridge containing an "e-liquid," which is a mixture of nicotine and flavoring in a glycerol-propylene glycol vehicle. Their increasing popularity among adolescents might be attributed to aggressive marketing in physical venues, social media outlets, as well as irreversible changes caused by nicotine in the developing brains of youth and young adults, predisposing them to addictive behaviors. Adolescent ENDS users were 4 times more likely to initiate cigarette smoking, and the ...
Source: Adv Dent Res - Category: Dentistry Authors: Tags: Adv Dent Res Source Type: research
Abstract Regulatory policy toward tobacco significantly affects oral health because tobacco use is a driver of diseases that manifest themselves in or near the oral cavity. Tobacco use in the United States has been associated with millions of cases of periodontal disease. Researchers have identified the role of combusted and noncombusted tobacco products in promoting cancers of the head and neck, leading to disease and premature death. Tobacco companies have moved increasingly toward so-called next-generation products (NGPs)-products that may emit fewer toxins than combustible forms of tobacco. Although NGPs may n...
Source: Adv Dent Res - Category: Dentistry Authors: Tags: Adv Dent Res Source Type: research
This article presents research on the architecture of Maggie's Centres, a series of buildings for those with cancer, their families and friends. In particular, we explore the way in which their architectural atmospheres are spoken of by architects who have designed individual Maggie's Centres, in interviews with staff members and volunteers in the buildings and in focus groups with visitors to their sites. We bring together qualitative research from two separate projects, and present findings from interviews, across the UK and internationally, with 66 visitors, 22 staff members and 7 architects of Maggie's Centres. How our...
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
More News: Cancer | Cancer & Oncology | Chemistry | Databases & Libraries | Genetics