Investigation of a 6-MSA Synthase Gene Cluster in Aspergillus aculeatus Reveals 6-MSA-derived Aculinic Acid, Aculins A-B and Epi-Aculin A.

In this study we identified a 6-methylsalicylic acid (6-MSA) synthase from A. aculeatus, and verified its functionality by episomal expression in A. aculeatus and heterologous expression in A. nidulans. Feeding studies with fully (13) C-labeled 6-MSA revealed that 6-MSA is incorporated into aculinic acid, which further incorporates into three compounds that we name aculins A and B, and epi-aculin A, described here for the first time. Based on NMR data and bioinformatic studies we propose the structures of the compounds as well as a biosynthetic pathway leading to formation of aculins from 6-MSA. PMID: 26374386 [PubMed - as supplied by publisher]
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research

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Publication date: February 2020Source: Steroids, Volume 154Author(s): Ambreen Aziz, Saira Bano, Atia-tul-Wahab, M. Iqbal ChoudharyAbstractEthisterone (17α-ethynyl-17β-hydroxyandrost-4-en-3-one) (1) is a synthetic steroidal estrogen. It is extensively used as an oral contraceptive. The current study involves the structural transformation of ethisterone (1) by Aspergillus niger, and Cunninghamella blakesleeana. Fermentation of 1 with C. blakesleeana afforded two new polar metabolites, 17α-ethynyl-6β,15β,17β-trihydroxyandrost-4-en-3-one, and 17α-ethynyl-7β,15β,17β-trihydrox...
Source: Steroids - Category: Drugs & Pharmacology Source Type: research
Conclusions: Overall results of surgery in this cohort were good and number of complications was low. Therapy with antifungals was diverse. Surgical treatment of aspergilloma can be life-saving for patients suffering of haemoptysis, and patients with restricted disease and well-preserved pulmonary capacity may benefit from surgery. Careful patient selection is crucial. PMID: 31737315 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Publication date: Available online 19 November 2019Source: American Journal of Infection ControlAuthor(s): Chloé Godeau, Gabriel Reboux, Emeline Scherer, Audrey Laboissiere, Clothilde Lechenault-Bergerot, Laurence Millon, Steffi RocchiScreening has been performed for azole-resistant Aspergillus fumigatus in the indoor air of the hospital since 2015 and in soil and dust samples since January 2019. In total, 83 azole-resistant A fumigatus isolates with a TR34/L98H mutation have been obtained: 1 from the air of the intensive care unit, 16 from the main corridors, 59 from pots of tulips imported from the Netherlands, an...
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Abstract Maize is an important staple crop for the majority of the population in Uganda. However, in tropical and subtropical climates, maize is frequently contaminated with aflatoxins, a group of cancer-causing and immuno-suppressive mycotoxins produced by Aspergillus section Flavi fungi. In Uganda, there is limited knowledge about the causal agents of aflatoxin contamination. The current study determined both the aflatoxin levels in pre-harvest maize across Uganda and the structures of communities of aflatoxin-producing fungi associated with the maize. A total of 256 pre-harvest maize samples were collected from...
Source: International Journal of Food Microbiology - Category: Food Science Authors: Tags: Int J Food Microbiol Source Type: research
Erratum for Gerke et al., "Breaking the Silence: Protein Stabilization Uncovers Silenced Biosynthetic Gene Clusters in the Fungus Aspergillus nidulans". Appl Environ Microbiol. 2019 Dec 01;85(23): Authors: Gerke J, Bayram Ö, Feussner K, Landesfeind M, Shelest E, Feussner I, Braus GH PMID: 31727853 [PubMed - in process]
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
lisotan Hakim Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona...
Source: Molecules - Category: Chemistry Authors: Tags: Communication Source Type: research
Conclusions: The PK simulations in this study suggest that transplant recipients on voriconazole prophylaxis against invasive candidiasis or aspergillosis are likely to achieve the target concentrations associated with the desired treatment outcomes if the maintenance dose is 200 mg twice daily. However, Aspergillus spp. with high minimal inhibitory concentrations could require higher maintenance doses.
Source: Therapeutic Drug Monitoring - Category: Drugs & Pharmacology Tags: Original Article Source Type: research
Publication date: Available online 17 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Qian Li, Christopher S. Ray, Nicholas V. Callow, Abdullah Al Loman, S.M. Mahfuzul Islam, Lu-Kwang JuAbstractSoybean is a most promising sustainable protein source for feed and food to help meet the protein demand of the rapidly rising global population. To enrich soy protein, the environment-friendly enzymatic processing requires multiple carbohydrases including cellulase, xylanase, pectinase, α-galactosidase and sucrase. Besides enriched protein, the processing adds value by generating monosaccharides that are ready ...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
In this study, a [Co(III)-EDTA]- resistant fungus was isolated from solar salt pan and was identified as Aspergillus versicolor SPF-1. Isolated fungus showed high value of minimum inhibitory concentration (MIC) towards Co-EDTA complexes, i.e. 17 mM and 22 mM for [Co(III)-EDTA]- and [Co(II)-EDTA]2- respectively. The fungal isolate took 5 days to remove 1 mM [Co(III)-EDTA]- from the bulk medium at pH 5.0 and 30 °C, however, maximum biosorption was observed at pH 4.0. SEM-EDAX analysis confirmed that A. versicolor SPF1 removed the [Co(III)-EDTA]- from bulk medium through biosorption mechanism.
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
AbstractItaconic acid (IA), a building block platform chemical, is produced industrially byAspergillus terreus utilizing glucose. Lignocellulosic biomass can serve as a low cost source of sugars for IA production. However, the fungus could not produce IA from dilute acid pretreated and enzymatically saccharified wheat straw hydrolyzate even at 100 ‐fold dilution. Furfural, hydroxymethyl furfural and acetic acid were inhibitory, as is typical, but Mn2+ was particularly problematic for IA production. It was present in the hydrolyzate at a level that was 230 times over the inhibitory limit (50  ppb). Recently, it was...
Source: Biotechnology Progress - Category: Biotechnology Authors: Tags: RESEARCH ARTICLE Source Type: research
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