Effect of mutations on the thermostability of Aspergillus aculeatus β-1,4-galactanase

In conclusion, this study tests a new method for estimating Tm changes for mutants, adds to the available data on the effect of substitutions on protein thermostability and identifies an interesting thermostabilizing mutation, which may be beneficial also in other galactanases. Graphical abstract
Source: Computational and Structural Biotechnology Journal - Category: Biotechnology Source Type: research

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In this study, a kind of Aspergillus fumigatus (LSD-1) was isolated from cigar leaves, and the biosorption effects on the stigmasterol, β-sitosterol, campesterol, cholesterol, and ergosterol by using living and dead biomass of LSD-1 were investigated. The results showed that both living and dead biomass could efficiently remove these sterols in aqueous solution and tobacco waste extract (TWE). Interestingly, compared with the living biomass of LSD-1, the dead biomass of LSD-1 not only kept a high adsorption efficiency but also did not produce ergosterol. Overall, dead biomass of LSD-1 was a more suitable biosorbent to...
Source: Bioscience, Biotechnology, and Biochemistry - Category: Biochemistry Authors: Tags: Biosci Biotechnol Biochem Source Type: research
The fungus Aspergillus sp. was isolated from copper mining rejects and cultured in a liquid culture medium (potato dextrose, PD) enriched with CuSO4.5H2O and Pb(NO3)2 in different concentrations. By flame atomic absorption spectrometry (FAAS) it was observed that fungus absorbed 86% of both copper and lead, with initial concentrations of 100, 500 and 1000 mg L-1. The additional results provided by scanning electron microscopy, energy dispersive spectroscopy, and X-ray diffraction showed ability of the microorganism to act as a biosorbent as well as its capacity to transform the tested salts into other inorganic compounds. ...
Source: Journal of the Brazilian Chemical Society - Category: Chemistry Source Type: research
People are constantly exposed to airborne fungal spores, including Aspergillus fumigatus conidia that can cause life-threatening conditions in immunocompromised patients or acute exacerbations in allergics. However, immunocompetent hosts do not exhibit mycoses or systemic inflammation, due to the sufficient but not excessive antifungal immune response that prevent fungal invasion. Intraepithelial dendritic cells (IE-DCs) of the conducting airway mucosa are located in the primary site of the inhalant pathogen entry; these cells can sense A. fumigatus conidia and maintain homeostasis. The mechanisms by which IE-DCs contribut...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 21 February 2020Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Khamis Al-Dhafri, Chai Lay Ching, Saiful Anuar KarsaniAbstractThe emergence of antibiotic resistance among bacteria leads to the urge of finding an alternative solution. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. This research was designed to explore the potency of antimicrobial peptides from Omani Juniperus seravschanica plant and their mode of action. The isolated AMPs showed to have inhibitory activity towards pathog...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
In conclusion, our results indicate that S. yanglinensis 3-10 may has a potential to become a promising biofumigant in for control of A. flavus and A. parasiticus.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Invasive pulmonary aspergillosis (IPA) remains difficult to diagnose and to treat. Most common risk factors are prolonged neutropenia, hematopoietic stem cell or solid organ transplantation, inherited or acquired immunodeficiency, administration of steroids or other immunosuppressive agents including monoclonal antibodies and new small molecules used for cancer therapy. Critically ill patients are also at high risk of IPA. Clinical signs are unspecific. Early computed tomography (CT)-scan identifies the two main aspects, angioinvasive and airway invasive aspergillosis. Although CT-scan findings are not fu...
Source: Respiratory Care - Category: Respiratory Medicine Authors: Tags: Semin Respir Crit Care Med Source Type: research
Semin Respir Crit Care Med 2020; 41: 080-098 DOI: 10.1055/s-0039-3401990Invasive pulmonary aspergillosis (IPA) remains difficult to diagnose and to treat. Most common risk factors are prolonged neutropenia, hematopoietic stem cell or solid organ transplantation, inherited or acquired immunodeficiency, administration of steroids or other immunosuppressive agents including monoclonal antibodies and new small molecules used for cancer therapy. Critically ill patients are also at high risk of IPA. Clinical signs are unspecific. Early computed tomography (CT)-scan identifies the two main aspects, angioinvasive and airway invasi...
Source: Seminars in Respiratory and Critical Care Medicine - Category: Respiratory Medicine Authors: Tags: Review Article Source Type: research
oltronieri : The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. The medicinal value of Moringa oleifera leaves and the antimicrobial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable added-value inorganic material. The use of a hydroalcoholic extract of M. oleifera leaves for the green synthesis of copper nanoparticles is an attractive method as it leads to the production of harmless chemicals and reduces waste. The total phenolic content in the M. oleifera leaves extract was 23.0 ± 0.3 mg...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
CONCLUSION: Our findings suggest that the crude AgCMCase is suitable to be used in straw conversion. PMID: 31980972 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research
Publication date: Available online 22 January 2020Source: Journal de Mycologie MédicaleAuthor(s): Arghadip Samaddar, Anuradha Sharma, Twishi ShrimaliAbstractAcrophialophora fusispora is a soil-borne fungus rarely implicated in human infections. Here, we report a case of pulmonary infection due to A. fusispora in a 59-year-old male who presented with productive cough and gradually progressive dyspnoea for 20 days. He had a past history of pulmonary tuberculosis and was a known case of chronic obstructive pulmonary disease for past five years. He was diagnosed with mixed connective tissue disease and had been receivin...
Source: Journal of Medical Mycology - Category: Biology Source Type: research
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