News at a glance: ALS drug setback, controls on AI protein design, and rebuilding Ukrainian labs
DRUG DEVELOPMENT
ALS drug comes up short in trial
In a major setback for people suffering from amyotrophic lateral sclerosis (ALS), a drug approved in the United States and Canada in 2022 for the debilitating condition has failed in a 664-patient clinical trial, its maker, Amylyx Pharmaceuticals, said last week. Regulators approved the therapy, a combination of two compounds, despite their concerns that the evidence it provided a clinical benefit in a smaller study was weak. But treatment options for ALS are few. Amylyx says it will now spend 8 weeks consulting regulators and those affected by ALS...
Source: Science of Aging Knowledge Environment - March 14, 2024 Category: Geriatrics Source Type: research
HPV Serology and Circulating Viral DNA for Detection, Genotyping, and Risk-Stratification in p16+ Oropharyngeal Cancer
The incidence of human papillomavirus-positive (HPV+) oropharyngeal cancer (OPC) has increased rapidly in North America. HPV serology has been proposed as a sensitive, scalable, and cost-effective early detection test. HPV seropositivity can precede clinical presentation of OPC by several years, so additional diagnostic and/or surveillance procedures may be necessary to optimize early cancer detection. The potential for HPV circulating tumor DNA (ctDNA) to confirm a diagnosis of OPC in seropositive individuals is poorly understood. (Source: International Journal of Radiation Oncology * Biology * Physics)
Source: International Journal of Radiation Oncology * Biology * Physics - March 14, 2024 Category: Radiology Authors: L. Penny, E. Stutheit-Zhao, Z. Zhao, Y. Zheng, J. Zou, S.H. Huang, J. de Almeida, D. Goldstein, A.J. Hope, A. Hosni, J. Kim, F.F. Liu, C.J. Tsai, J. Waldron, A. Spreafico, E. Sanz Garcia, L.L. Siu, T. Waterboer, G. Liu, S.V. Bratman Tags: 5 Source Type: research
Clinical Outcomes of Human papillomavirus (HPV) Testing Discordance in Oropharyngeal Squamous cell Carcinoma
In this study, we analyzed the discordance between p16 IHC and HPV status in patients treated with definitive (chemo)radiation (CRT) for OPSCC and any potential association with treatment outcome. (Source: International Journal of Radiation Oncology * Biology * Physics)
Source: International Journal of Radiation Oncology * Biology * Physics - March 14, 2024 Category: Radiology Authors: R.F. Shenker, J. Saifullan, J. Canick, X. Jiang, Z. Wan, D. Niedzwiecki, R.T. Hughes, Y.M. Mowery, D.M. Brizel Tags: 106 Source Type: research
Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors
Front Cell Infect Microbiol. 2024 Feb 28;14:1357090. doi: 10.3389/fcimb.2024.1357090. eCollection 2024.ABSTRACTCervical cancer is the third most common cancer threatening women's health globally, and high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer worldwide. Given the recurrent nature of HR-HPV infection, accurate screening is essential for its control. Since the commonly used polymerase chain reaction (PCR) technique is limited by professional equipment and personnel, convenient and ultrasensitive detection methods for HR-HPV are still highly needed. As new molecular detection method...
Source: Rural Remote Health - March 14, 2024 Category: Rural Health Authors: Chang Ma Minhong Zou Ning Xu Yan Liu Yuru Wang Source Type: research
Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors
Front Cell Infect Microbiol. 2024 Feb 28;14:1357090. doi: 10.3389/fcimb.2024.1357090. eCollection 2024.ABSTRACTCervical cancer is the third most common cancer threatening women's health globally, and high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer worldwide. Given the recurrent nature of HR-HPV infection, accurate screening is essential for its control. Since the commonly used polymerase chain reaction (PCR) technique is limited by professional equipment and personnel, convenient and ultrasensitive detection methods for HR-HPV are still highly needed. As new molecular detection method...
Source: Rural Remote Health - March 14, 2024 Category: Rural Health Authors: Chang Ma Minhong Zou Ning Xu Yan Liu Yuru Wang Source Type: research
Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors
Front Cell Infect Microbiol. 2024 Feb 28;14:1357090. doi: 10.3389/fcimb.2024.1357090. eCollection 2024.ABSTRACTCervical cancer is the third most common cancer threatening women's health globally, and high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer worldwide. Given the recurrent nature of HR-HPV infection, accurate screening is essential for its control. Since the commonly used polymerase chain reaction (PCR) technique is limited by professional equipment and personnel, convenient and ultrasensitive detection methods for HR-HPV are still highly needed. As new molecular detection method...
Source: Rural Remote Health - March 14, 2024 Category: Rural Health Authors: Chang Ma Minhong Zou Ning Xu Yan Liu Yuru Wang Source Type: research
Value of high ‑risk human papillomavirus detection combined with colposcopy in the diagnosis of cervical cancer and precancerous lesions
Oncol Lett. 2024 Feb 29;27(4):185. doi: 10.3892/ol.2024.14318. eCollection 2024 Apr.ABSTRACTIn the present study, the diagnostic value of high risk-human papillomavirus (HR-HPV) combined with colposcopy for the detection of cervical cancer and precancerous lesions was evaluated. A total of 397 patients with confirmed cervical disease were enrolled between August 2020 and December 2021. According to the pathological diagnosis, the patients were divided into cervical intraepithelial neoplasia grade I (CIN I; n=153 cases), CIN II (n=101 cases), CIN III (n=86 cases) and cervical cancer (n=57 cases) groups. The HR-HPV-positive ...
Source: Oncology Letters - March 13, 2024 Category: Cancer & Oncology Authors: Ping Wang Dongxia Gao Xiaoni Yu Gaoxiang Zhu Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Mol Biol Cell - March 8, 2024 Category: Molecular Biology Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Cellular and Molecular Biology Letters - March 8, 2024 Category: Biochemistry Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Cancer Control - March 8, 2024 Category: Cancer & Oncology Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Cellular and Molecular Biology Letters - March 8, 2024 Category: Biochemistry Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Cellular and Molecular Biology Letters - March 8, 2024 Category: Biochemistry Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay
This study, guided by the genomic sequence patterns of HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on the concept of "universal primers." This approach achieved the multiple amplification of RPA, coupled with the CRISPR/Cas12a system serving as a medium for signal amplification and conversion. The study successfully constructed a POCT combined detection system, denoted as H-MRC12a (HPV-Multiple RPA-CRISPR/Cas12a), and applied it to high-risk HPV typing detection. The system accomplished the typing detection of six high-risk HPV types (16, 18, 31, 33, 35, and 45) can be completed...
Source: Cellular and Molecular Biology Letters - March 8, 2024 Category: Biochemistry Authors: Yan Liu Zhujun Chao Wei Ding Tanfeng Fang Xinxian Gu Man Xue Wei Wang Rong Han Wanping Sun Source Type: research
