Viruses, Vol. 16, Pages 298: Association between Human Papillomavirus 16 Viral Load in Pregnancy and Preterm Birth
This study aimed to measure the association between HPV16 viral load during pregnancy and preterm birth. We used data from participants in the HERITAGE study. The Linear Array assay was used for HPV DNA testing on vaginal samples collected during the first and third trimesters of pregnancy. The HPV16 viral load was measured with a real-time polymerase chain reaction. We used logistic regression to measure the associations between HPV16 viral load during pregnancy and preterm birth (defined as birth before 37 weeks of gestation). The adjusted odd ratios (aORs) and the 95% confidence intervals [CIs] were estimated with inver...
Source: Viruses - February 15, 2024 Category: Virology Authors: Pranamika Khayargoli Marie-H élène Mayrand Joseph Niyibizi Fran çois Audibert Louise Laporte Julie Lacaille Ana Maria Carceller Jacques Lacroix Émilie Comète Fran çois Coutlée Trottier Tags: Article Source Type: research
Assessing the feasibility of a multimodal liquid biopsy for the diagnosis of HPV-associated oropharyngeal squamous cell carcinoma
CONCLUSIONS: Compared to HPV RNA ISH, HPV serology and ctHPVDNA are sensitive and highly specific biomarkers for HPV-associated OPSCC at the time of presentation.PMID:38349613 | DOI:10.1093/ajcp/aqad185 (Source: American Journal of Clinical Pathology)
Source: American Journal of Clinical Pathology - February 13, 2024 Category: Pathology Authors: James S Lewis Saskia Naegele Vasileios Efthymiou Mitra Mehrad Kim A Ely Tim Waterboer Krystle A Lang Kuhs Seth J Davis Kelsey Richard Dipon Das Daniel L Faden Source Type: research
Assessing the feasibility of a multimodal liquid biopsy for the diagnosis of HPV-associated oropharyngeal squamous cell carcinoma
CONCLUSIONS: Compared to HPV RNA ISH, HPV serology and ctHPVDNA are sensitive and highly specific biomarkers for HPV-associated OPSCC at the time of presentation.PMID:38349613 | DOI:10.1093/ajcp/aqad185 (Source: American Journal of Clinical Pathology)
Source: American Journal of Clinical Pathology - February 13, 2024 Category: Pathology Authors: James S Lewis Saskia Naegele Vasileios Efthymiou Mitra Mehrad Kim A Ely Tim Waterboer Krystle A Lang Kuhs Seth J Davis Kelsey Richard Dipon Das Daniel L Faden Source Type: research
Assessing the feasibility of a multimodal liquid biopsy for the diagnosis of HPV-associated oropharyngeal squamous cell carcinoma
CONCLUSIONS: Compared to HPV RNA ISH, HPV serology and ctHPVDNA are sensitive and highly specific biomarkers for HPV-associated OPSCC at the time of presentation.PMID:38349613 | DOI:10.1093/ajcp/aqad185 (Source: American Journal of Clinical Pathology)
Source: American Journal of Clinical Pathology - February 13, 2024 Category: Pathology Authors: James S Lewis Saskia Naegele Vasileios Efthymiou Mitra Mehrad Kim A Ely Tim Waterboer Krystle A Lang Kuhs Seth J Davis Kelsey Richard Dipon Das Daniel L Faden Source Type: research
A validated polyclonal antiserum-based immunoassay for assessment of HPV 16 L1 relative potency
Biologicals. 2024 Feb 8;85:101742. doi: 10.1016/j.biologicals.2023.101742. Online ahead of print.ABSTRACTVaccine potency is typically evaluated using an assay that acts as a surrogate for biological activity. Although in vivo vaccines better represent human immunological responses, in vitro assays are preferred due to lower variability, higher throughput, easier validation and ethical considerations. In in vitro determination of Human Papillomavirus (HPV), Virus-like particle (VLP) vaccine potency currently depends on monoclonal antibody assays. However, these reagents are hard to obtain and currently are not available com...
Source: Biologicals : Journal of the International Association of Biological Standardization - February 10, 2024 Category: Biotechnology Authors: Reihaneh Mirhassani Ehsan Seyedjafari Behrouz Vaziri Source Type: research
Single-tube four-target lateral flow assay detects human papillomavirus types associated with majority of cervical cancers
In this study, we developed a multiplexed loop-mediated isothermal amplification (LAMP) reaction to detect the three most common hrHPV types that cause cervical cancer (HPV16, HPV18, and HPV45) and a cellular control for sample adequacy. First, we characterized the assay limit of detection (LOD) in a real-time reaction with fluorescence readout; after 30 min of amplification the LOD was 100, 10, and 10 copies/reaction of HPV16, HPV18, and HPV45, respectively, and 0.1 ng/reaction of human genomic DNA (gDNA). Next, we implemented the assay on lateral flow strips, and the LOD was maintained for HPV16 and HPV18, but increased ...
Source: Analytical Biochemistry - February 8, 2024 Category: Biochemistry Authors: Maria Barra Megan Chang Mila P Salcedo Kathleen Schmeler Michael Scheurer Mauricio Maza Leticia Lopez Karla Alfaro Rebecca Richards-Kortum Source Type: research
Impact of human papillomavirus age-related prevalence and vaccination levels on interpretation of cervical screening modalities: a modelling study
Conclusions
HPV-reflex tests outperform LBC tests across all age profiles, resulting in greater test accuracy. This improvement is especially pronounced as HPV infection rates fall and suggests HPV-reflex modalities are robust to future changes in the epidemiology of HPV. (Source: BMJ Open)
Source: BMJ Open - February 2, 2024 Category: General Medicine Authors: Grimes, D. R. Tags: Open access, Public health Source Type: research
News at a glance: Mars helicopter ’s final flight, grants for U.K. diversity, and a nonopioid pain drug
PLANETARY SCIENCE
Breakdown finally grounds Mars helicopter
After 72 flights on Mars—67 more than originally planned—NASA’s Ingenuity helicopter flew its final sortie last month. The tiny helicopter (above), a technology demonstration deployed from the Perseverance rover, lasted for 3 years, covering 17 kilometers during more than 2 hours of flight, until at least one of its carbon-fiber blades fractured during a crash landing. Ingenuity served as a scout for Perseverance as it climbed toward the rim of Jezero crater, helping it avoid hazardous terrain and collect rock samples. The mission coul...
Source: Science of Aging Knowledge Environment - February 1, 2024 Category: Geriatrics Source Type: research
Calendar-period trends in cervical precancer and cancer diagnoses since the introduction of human papillomavirus and cytology co-testing into routine cervical cancer screening at Kaiser Permanente Northern California
CONCLUSIONS: Since its introduction at KPNC, co-testing increased the detection of CIN3 over time, which likely caused a subsequent reduction of SCC. However, there has been no observed decrease in ADC. One possible explanation for lack of effectiveness against ADC is the underdiagnosis of AIS. Novel strategies to identify and treat women at high risk of ADC need to be developed and clinically validated.PMID:38301311 | DOI:10.1016/j.ygyno.2024.01.038 (Source: Gynecologic Oncology)
Source: Gynecologic Oncology - February 1, 2024 Category: Cancer & Oncology Authors: Brian Befano Nicolas Wentzensen Thomas Lorey Nancy Poitras Li C Cheung Mark Schiffman Megan A Clarke Camryn Cohen Walter Kinney Alex Locke Philip E Castle Source Type: research
Variation in cervical cancer screening test utilization and results in a United States-based program
CONCLUSIONS: Cervical cancer screening strategy utilization and test results vary substantially by geographic region within a national screening program. Variation in utilization may be due to regional differences in screening test availability or the preferences of healthcare systems, screened persons and/or clinicians. Test result variations may reflect differing risk factors for HPV infections by geographic region.PMID:38301312 | DOI:10.1016/j.ygyno.2024.01.020 (Source: Gynecologic Oncology)
Source: Gynecologic Oncology - February 1, 2024 Category: Cancer & Oncology Authors: Vanessa G Dorismond Mona Saraiya Sameer V Gopalani Ashwini Soman Kristy Kenney Jacqueline Miller George F Sawaya Source Type: research
Opportunistic HPV test outcomes
Human papillomavirus vaccination is not in the vaccination programme in Turkey. The 9-valent HPV vaccine just started in January 2023 and is not covered by the government, while all other vaccines are. Pap-smear and HPV co-tests are routinely done by the family medicine and opportunistic tests are done by the gynecologists. We wanted to check rates of opportunistic HPV tests that were collected in our private Safa hospital, results of these tests, and if patients were vaccinated before or after this testing in the past five years. (Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology)
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - February 1, 2024 Category: OBGYN Authors: Elif Goknur Topcu Source Type: research
Stratified Mucin-Producing Intraepithelial Lesion of the Cervix in an HPV- 16 Positive Woman: A Rare Encounter
CONCLUSION: SMILE is a rare variant of AIS, which is often missed on cytological screening of the cervix. It is commonly associated with high-risk types of HPV. Hence, incorporating HPV testing in the screening of cervical cancer is important and recommended to increase the overall sensitivity of screening for adenocarcinoma lesions.PMID:38288809 | DOI:10.2174/0118715265284722240110114107 (Source: Infectious Disorders Drug Targets)
Source: Infectious Disorders Drug Targets - January 30, 2024 Category: Infectious Diseases Authors: Naina Kumar Mishu Mangla Seetu Palo Spandana Gabbeta J Aparna Source Type: research
Human papillomavirus and cervical cancer in the microbial world: exploring the vaginal microecology
The vaginal microbiota plays a crucial role in female reproductive health and is considered a biomarker for predicting disease outcomes and personalized testing. However, its relationship with human papillomavirus (HPV) infection and cervical cancer is not yet clear. Therefore, this article provides a review of the association between the vaginal microbiota, HPV infection, and cervical cancer. We discuss the composition of the vaginal microbiota, its dysbiosis, and its relationship with HPV infection, as well as potential mechanisms in the development of cervical cancer. In addition, we assess the feasibility of treatment ...
Source: Frontiers in cellular and infection microbiology - January 25, 2024 Category: Microbiology Source Type: research
Human papillomavirus E7 protein induces homologous recombination defects and PARPi sensitivity
ConclusionIn aggregate, our findings demonstrate that HPV-E7 is a potential driver for genome instability and provides a new angle to understand its role in cancer development. The viral HRD could be employed to target HPV-positive cervical cancer via synthetic lethality. (Source: Journal of Cancer Research and Clinical Oncology)
Source: Journal of Cancer Research and Clinical Oncology - January 23, 2024 Category: Cancer & Oncology Source Type: research
A systematic literature review of the human papillomavirus prevalence in locally and regionally advanced and recurrent/metastatic head and neck cancers through the last decade: The “ALARM” study
ConclusionOver the last decade, at least one quarter of LA/RM HNC and half of OPC cases studied in IS and NIS were HPV+. This alarming burden is consistent with a potential implication of HPV in the pathogenesis of at least a subgroup of HNC, underscoring the relevance of HPV testing and prophylaxis to HNC prevention and management. (Source: Cancer Medicine)
Source: Cancer Medicine - January 22, 2024 Category: Cancer & Oncology Authors: Sofia Agelaki,
Ioannis Boukovinas,
Ilias Athanasiadis,
Georgios Trimis,
Ioannis Dimitriadis,
Lazaros Poughias,
Edith Morais,
Ugne Sabale,
Goran Bencina,
Charalampos Athanasopoulos Tags: REVIEW Source Type: research
