Prognostic factors for the outcomes of COVID-19 patients infected with SARS-CoV-2 Omicron and Delta variants
The SARS-CoV-2 Omicron variant has replaced the previously dominant Delta variant because of high transmissibility. However, studies on the impact of the Omicron variant on the severity of COVID-19 are still l... (Source: BMC Medical Genomics)
Source: BMC Medical Genomics - August 29, 2023 Category: Genetics & Stem Cells Authors: Gunadi, Mohamad Saifudin Hakim, Hendra Wibawa, Khanza Adzkia Vujira, Dyah Ayu Puspitarani, Endah Supriyati, Ika Trisnawati, Kristy Iskandar, Riat El Khair, Afiahayati, Siswanto, Yunika Puspadewi, Sri Handayani Irianingsih, Dwi Aris Agung Nugrahaningsih, L Tags: Research Source Type: research
Cell-Cell Fusion Assays to Study Henipavirus Entry and Evaluate Therapeutics
Methods Mol Biol. 2023;2682:59-69. doi: 10.1007/978-1-0716-3283-3_4.ABSTRACTHenipaviruses include the deadly zoonotic Nipah (NiV) and Hendra (HeV) paramyxoviruses, which have caused recurring outbreaks in human populations. A hallmark of henipavirus infection is the induction of cell-cell fusion (syncytia), caused by the expression of the attachment (G) and fusion (F) glycoproteins on the surface of infected cells. The interactions of G and F with each other and with receptors on cellular plasma membranes drive both viral entry and syncytia formation and are thus of great interest. While F shares structural and functional ...
Source: Mol Biol Cell - August 23, 2023 Category: Molecular Biology Authors: I Abrrey Monreal Hector C Aguilar Source Type: research
In Vitro Antiviral Screening for Henipaviruses at BSL4
Methods Mol Biol. 2023;2682:93-102. doi: 10.1007/978-1-0716-3283-3_7.ABSTRACTIn vitro screening for antivirals is an essential step in the development of effective treatments against new and emerging pathogens. Here, we describe a simple, cell-based screening assay for evaluating antiviral effectiveness against Hendra and Nipah live virus infection under BSL4 conditions.PMID:37610576 | DOI:10.1007/978-1-0716-3283-3_7 (Source: Mol Biol Cell)
Source: Mol Biol Cell - August 23, 2023 Category: Molecular Biology Authors: Bruce A Mungall Source Type: research
Henipavirus-induced neuropathogenesis in mice
Virology. 2023 Jul 29;587:109856. doi: 10.1016/j.virol.2023.109856. Online ahead of print.ABSTRACTHendra virus (HeV) and Nipah virus (NiV) are henipaviruses that can cause fatal encephalitis in humans. Many animal models have been used to study henipavirus pathogenesis. In the mouse, HeV infection has previously shown that intranasal challenge can lead to neurological infection, however mice similarly challenged with NiV show no evidence of virus infecting the brain. We generated recombinant HeV (rHeV) and NiV (rNiV) where selected proteins were switched to examine their role in neuroinvasion in the mouse. These viruses di...
Source: Virology - August 4, 2023 Category: Virology Authors: Sarah J Edwards Brenton Rowe Tristan Reid Mary Tachedjian Sarah Caruso Kim Blasdell Shumpei Watanabe Jemma Bergfeld Glenn A Marsh Source Type: research
Nipah virus attachment glycoprotein ectodomain delivered by type 5 adenovirus vector elicits broad immune response against NiV and HeV
In this study, the full-length protein (G), the ectodomain (Ge) and the head domain (Gs) of NiV attachment glycoprotein were delivered by the replication-defective type 5 adenovirus vector (Ad5) respectively, and the recombinant Ad5-NiV vaccine candidates (Ad5-NiVG, Ad5-NiVGe and Ad5-NiVGs) were constructed and their immunogenicity were evaluated in mice. The results showed that all the vaccine candidates stimulated specific humoral and cellular immune responses efficiently and rapidly against both NiV and HeV, and the Ad5-NiVGe elicited the strongest immune responses after a single-dose immunization. Furthermore, the pote...
Source: Frontiers in cellular and infection microbiology - July 27, 2023 Category: Microbiology Source Type: research
Henipavirus zoonosis: outbreaks, animal hosts and potential new emergence
Hendra virus (HeV) and Nipah virus (NiV) are biosafety level 4 zoonotic pathogens causing severe and often fatal neurological and respiratory disease. These agents have been recognized by the World Health Organization as top priority pathogens expected to result in severe future outbreaks. HeV has caused sporadic infections in horses and a small number of human cases in Australia since 1994. The NiV Malaysia genotype (NiV-M) was responsible for the 1998–1999 epizootic outbreak in pigs with spillover to humans in Malaysia and Singapore. Since 2001, the NiV Bangladesh genotype (NiV-B) has been the predominant strain leadin...
Source: Frontiers in Microbiology - July 17, 2023 Category: Microbiology Source Type: research
Small Molecule Inhibitors of Human Papillomavirus: A Review of Research from 1997 to 2021
Curr Med Chem. 2023 Jul 13. doi: 10.2174/0929867331666230713165407. Online ahead of print.ABSTRACTHuman papillomavirus (HPV) infections are the cause of warts, lesions and cancer, with different types of HPV causing different symptoms. HPV infections are the primary cause of cervical cancer. There are over 220 different types of HPV, and only nine of these can currently be vaccinated. There is a need to treat these viral infections without just treating the symptoms of the infection, as is currently the main method. There is a wide range of small molecules that have been used to inhibit various stages of the HPV infectious...
Source: Current Medicinal Chemistry - July 14, 2023 Category: Chemistry Authors: Caitlin L Duncan Hendra Gunosewoyo Mauro Mocerino Alan D Payne Source Type: research
Small Molecule Inhibitors of Human Papillomavirus: A Review of Research from 1997 to 2021
Curr Med Chem. 2023 Jul 13. doi: 10.2174/0929867331666230713165407. Online ahead of print.ABSTRACTHuman papillomavirus (HPV) infections are the cause of warts, lesions and cancer, with different types of HPV causing different symptoms. HPV infections are the primary cause of cervical cancer. There are over 220 different types of HPV, and only nine of these can currently be vaccinated. There is a need to treat these viral infections without just treating the symptoms of the infection, as is currently the main method. There is a wide range of small molecules that have been used to inhibit various stages of the HPV infectious...
Source: Current Medicinal Chemistry - July 14, 2023 Category: Chemistry Authors: Caitlin L Duncan Hendra Gunosewoyo Mauro Mocerino Alan D Payne Source Type: research
A Retrospective Study on the Clinical, Laboratory, and Nutritional Status of Pediatric Epidermolysis Bullosa in a Tertiary Referral Hospital in West Java, Indonesia
CONCLUSION: RDEB is the most commonly found type of pediatric EB. Wounds on the skin, tooth decay, hand deformity, pain when changing dressings, low zinc levels, and low hemoglobin levels are the clinical features and laboratory findings that contribute to the development of moderate and severe malnutrition in RDEB patients.PMID:37389358 | PMC:PMC10305771 | DOI:10.2147/CCID.S413093 (Source: Clinical, Cosmetic and Investigational Dermatology)
Source: Clinical, Cosmetic and Investigational Dermatology - June 30, 2023 Category: Dermatology Authors: Laila Tsaqilah Inne Arline Diana Srie Prihianti Gondokaryono Raden Mohamad Rendy Ariezal Effendi Oki Suwarsa Hendra Gunawan Risa Milliawati Nurul Hidayah Reiva Farah Dwiyana Source Type: research
Gene Expression of Human Beta-Defensin-3 and Cathelicidin in the Skin of Leprosy Patients, Household Contacts, and Healthy Individuals from Indonesia
CONCLUSION: Gene expression of HBD-3 and cathelicidin increased in skin lesions of leprosy patients and household contacts.PMID:37333516 | PMC:PMC10275412 | DOI:10.2147/CCID.S405932 (Source: Clinical, Cosmetic and Investigational Dermatology)
Source: Clinical, Cosmetic and Investigational Dermatology - June 19, 2023 Category: Dermatology Authors: Fifa Argentina Oki Suwarsa Hendra Gunawan Afiat Berbudi Source Type: research
Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
Colloids Surf B Biointerfaces. 2023 Jun 2;228:113390. doi: 10.1016/j.colsurfb.2023.113390. Online ahead of print.ABSTRACTPostoperative bleeding following cardiac surgeries is still an issue that deranges the medical resources and cost. The oral and injection administrations of blood coagulation protein, Factor VII (FVII), is effective to stop the bleeding. However, its short half-life has limited the effectiveness of this treatment and frequent FVII intake may distress the patients. Instead, incorporating FVII into synthetic biodegradable polymers such as polycaprolactone (PCL) that is commonly used in drug delivery applic...
Source: Colloids and Surfaces - June 14, 2023 Category: Biotechnology Authors: Rathosivan Gopal Muhammad Faiz Md Shakhih Mariaulpa Sahalan Te Chuan Lee Hendra Hermawan Sivakumar Sivalingam Suhaini Kadiman Syafiqah Saidin Source Type: research
GSE234213 The Sequence Basis for Selectivity of Ephrin-B2 for Eph Receptors and Pathogenic Henipavirus G Glycoproteins [EFNB2 DEEP MUTAGENESIS]
Contributor : Erik ProckoSeries Type : OtherOrganism : Homo sapiensEphrin-B2 (EFNB2) is a ligand for six Eph receptors in humans and also functions as a cell entry receptor for several henipaviruses, which are zoonotic viruses with pandemic potential. Two such viruses, Nipah (NiV) and Hendra (HeV), are highly pathogenic in humans yet do not have any widely approved virus-specific therapeutics or vaccines. Soluble Fc-fusions of EFNB2 (sEFNB2-Fc) are known to bind the attachment glycoprotein G of both viruses and inhibit viral infection of cells. However, the potential of sEFNB2-Fc as a neutralizing agent in vivo is hindere...
Source: GEO: Gene Expression Omnibus - June 12, 2023 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research
