Viruses, Vol. 16, Pages 626: Detection and Characterization of Influenza A Virus Endemic Circulation in Suckling and Nursery Pigs Originating from Vaccinated Farms in the Same Production System
The objective of this study was to evaluate the detection of IAV in suckling and nursery piglets from IAV-vaccinated sows from farms with endemic IAV infections. Eight nasal swab collections were obtained from 135 two-week-old suckling piglets from four farms every other week from March to September 2013. Oral fluid samples were collected from the same group of nursery piglets. IAV RNA was detected in 1.64% and 31.01% of individual nasal swabs and oral fluids, respectively. H1N2 was detected most often, with sporadic detection of H1N1 and H3N2. Whole-genome sequences of IAV isolated from suckling piglets revealed an H1 hem...
Source: Viruses - April 18, 2024 Category: Virology Authors: Alessandra Silva Dias Amy L. Vincent Baker Rodney B. Baker Jianqiang Zhang Michael A. Zeller Pravina Kitikoon Phillip C. Gauger Tags: Article Source Type: research
Synthesis of Rupestonic Acid L-Ephedrine Derivatives with Preliminary In vitro Anti-influenza Viral Activity
CONCLUSION: The rupestonic acid L-ephedrine ester (A) and rupestonic acid L-ephedrine complex (B) were synthesized and characterized using 1H NMR and 13C NMR. Moreover, their purity was determined by HPLC. Both compounds A and B exhibited more potent activities against the strains of A/PR/8/34 (H1N1) and A/FM/1/47 (H1N1) than rupestonic acid. Compound A can be regarded as a very promising lead compound for the development of anti-influenza inhibitors. Based on these results, more rupestonic acid derivatives will be designed and synthesized in the future for the development of anti-influenza inhibitors.PMID:38623973 | DOI:1...
Source: Current Pharmaceutical Design - April 16, 2024 Category: Drugs & Pharmacology Authors: Jianping Yong Canzhong Lu Source Type: research
Synthesis of Rupestonic Acid L-Ephedrine Derivatives with Preliminary In vitro Anti-influenza Viral Activity
CONCLUSION: The rupestonic acid L-ephedrine ester (A) and rupestonic acid L-ephedrine complex (B) were synthesized and characterized using 1H NMR and 13C NMR. Moreover, their purity was determined by HPLC. Both compounds A and B exhibited more potent activities against the strains of A/PR/8/34 (H1N1) and A/FM/1/47 (H1N1) than rupestonic acid. Compound A can be regarded as a very promising lead compound for the development of anti-influenza inhibitors. Based on these results, more rupestonic acid derivatives will be designed and synthesized in the future for the development of anti-influenza inhibitors.PMID:38623973 | DOI:1...
Source: Current Pharmaceutical Design - April 16, 2024 Category: Drugs & Pharmacology Authors: Jianping Yong Canzhong Lu Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Cell Research)
Source: Cell Research - April 15, 2024 Category: Cytology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Design, synthesis, docking, and antiviral evaluation of some novel pyrimidinone-based α-aminophosphonates as potent H1N1 and HCoV-229E inhibitors
Bioorg Chem. 2024 Apr 8;147:107353. doi: 10.1016/j.bioorg.2024.107353. Online ahead of print.ABSTRACTDialkyl/aryl aminophosphonates, 3a-g and 4a-e were synthesized using the LiClO4 catalyzed Kabachnic Fields-type reaction straightforwardly and efficiently. The synthesized phosphonates structures were characterized using elemental analyses, FT-IR, 1H NMR, 13C NMR, and MS spectroscopy. The new compounds were subjected to in-silico molecular docking simulations to evaluate their potential inhibition against Influenza A Neuraminidase and RNA-dependent RNA polymerase of human coronavirus 229E. Subsequently, the compounds were f...
Source: Bioorganic Chemistry - April 14, 2024 Category: Chemistry Authors: Hend A Hekal Omar M Hammad Nasser R El-Brollosy Maha M Salem Abdou K Allayeh Source Type: research
A sensitive fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies to detect the H1N1 virus
Anal Bioanal Chem. 2024 Apr 13. doi: 10.1007/s00216-024-05269-x. Online ahead of print.ABSTRACTWe propose a sensitive H1N1 virus fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies. Products are generated via the hybridization of single-stranded DNA (ssDNA) probes containing T7 promoter and crRNA templates to a target RNA sequence using SplintR ligase. This generates large crRNA quantities in the presence of T7 RNA polymerase. At such crRNA quantities, ternary Cas13a, crRNA, and activator complexes are successfully constructed and activate Cas13a to enhance flu...
Source: Analytical and Bioanalytical Chemistry - April 13, 2024 Category: Chemistry Authors: Lulu Xue Shengjun Bu Mengyao Xu Jiaqi Wei Hongyu Zhou Yao Xu Zhuo Hao Zehong Li Jiayu Wan Source Type: research
A sensitive fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies to detect the H1N1 virus
Anal Bioanal Chem. 2024 Apr 13. doi: 10.1007/s00216-024-05269-x. Online ahead of print.ABSTRACTWe propose a sensitive H1N1 virus fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies. Products are generated via the hybridization of single-stranded DNA (ssDNA) probes containing T7 promoter and crRNA templates to a target RNA sequence using SplintR ligase. This generates large crRNA quantities in the presence of T7 RNA polymerase. At such crRNA quantities, ternary Cas13a, crRNA, and activator complexes are successfully constructed and activate Cas13a to enhance flu...
Source: Analytical and Bioanalytical Chemistry - April 13, 2024 Category: Chemistry Authors: Lulu Xue Shengjun Bu Mengyao Xu Jiaqi Wei Hongyu Zhou Yao Xu Zhuo Hao Zehong Li Jiayu Wan Source Type: research
Viruses, Vol. 16, Pages 598: Avian Influenza Virus and Avian Paramyxoviruses in Wild Waterfowl of the Western Coast of the Caspian Sea (2017 & ndash;2020)
Viruses, Vol. 16, Pages 598: Avian Influenza Virus and Avian Paramyxoviruses in Wild Waterfowl of the Western Coast of the Caspian Sea (2017–2020)
Viruses doi: 10.3390/v16040598
Authors:
Murashkina
Sharshov
Gadzhiev
Petherbridge
Derko
Sobolev
Dubovitskiy
Loginova
Kurskaya
Kasianov
Kabilov
Mine
Uchida
Tsunekuni
Saito
Alekseev
Shestopalov
The flyways of many different wild waterfowl pass through the Caspian Sea region. The western coast of the middle Caspian Sea is an area with many wetlands, where wintering grounds with large concentrations of birds are loca...
Source: Viruses - April 12, 2024 Category: Virology Authors: Murashkina Sharshov Gadzhiev Petherbridge Derko Sobolev Dubovitskiy Loginova Kurskaya Kasianov Kabilov Mine Uchida Tsunekuni Saito Alekseev Shestopalov Tags: Article Source Type: research
Pilot-scale production of a highly efficacious and stable monoglycosylated influenza split virus vaccine
In conclusion, this study provides efficacy and safety profiles of IVR-190mg for further clinical study and shows that this vaccine without a glycan shield has great potential to be safe and protective against H1N1 variants.PMID:38582606 | DOI:10.1016/j.vaccine.2023.11.038 (Source: Vaccine)
Source: Vaccine - April 6, 2024 Category: Allergy & Immunology Authors: Chia-Ying Wu Shao-En Kao Yung-Chieh Tseng Jen-Tzu Hou Li-Yang Wu Juine-Ruey Chen Source Type: research
Preparation and characterization of curdlan-chitosan conjugate nanoparticles as mucosal adjuvants for intranasal influenza H1N1 subunit vaccine
In this study, stable nanoparticles formed by curdlan-O-HTCC conjugate (CO NP) were prepared and characterized. Furthermore, the efficacy of CO NP was evaluated as a mucosal adjuvant in an intranasal influenza H1N1 subunit vaccine. The results revealed that CO NP exhibits uniform and spherical morphology, with a size of 190.53 ± 4.22 nm, and notably, it remains stable in PBS at 4 °C for up to 6 weeks. Biological evaluation demonstrated that CO NP stimulates the activation of antigen-presenting cells (APCs), including macrophages and dendritic cells (DCs), both in vitro and in vivo. Furthermore, intranasal administration ...
Source: International Journal of Biological Macromolecules - April 3, 2024 Category: Biochemistry Authors: Honglei Jiang Shu Zhang Yipan Chen Fengshan Wang Wenjie Jiang Source Type: research
Evaluation of disease severity and prediction of severe cases in children hospitalized with influenza A (H1N1) infection during the post-COVID-19 era: a multicenter retrospective study
The rebound of influenza A (H1N1) infection in post-COVID-19 era recently attracted enormous attention due the rapidly increased number of pediatric hospitalizations and the changed characteristics compared to... (Source: BMC Pediatrics)
Source: BMC Pediatrics - April 2, 2024 Category: Pediatrics Authors: Hai-Feng Liu, Xiao-Zhong Hu, Rong-Wei Huang, Zheng-Hong Guo, Jin-Rong Gao, Mei Xiang, Rui Lu, Deng Ban, Cong-Yun Liu, Ya-Yu Wang, Wang Li, Yin Li, Yun-Jie Guo, Quan Lu and Hong-Min Fu Tags: Research Source Type: research
Supplementation of seasonal vaccine with multi-subtype neuraminidase and M2 ectodomain virus-like particle improves protection against homologous and heterologous influenza viruses in aged mice
Antiviral Res. 2024 Mar 30:105877. doi: 10.1016/j.antiviral.2024.105877. Online ahead of print.ABSTRACTThe conventional inactivated split seasonal influenza vaccine offers low efficacy, particularly in the elderly and against antigenic variants. Here, to improve the efficacy of seasonal vaccination for the elderly population, we tested whether supplementing seasonal bivalent (H1N1 + H3N2) split (S) vaccine with M2 ectodomain repeat and multi-subtype consensus neuraminidase (NA) proteins (N1 NA + N2 NA + flu B NA) on a virus-like particle (NA-M2e) would induce enhanced cross-protection against different influenza viruses in...
Source: Antiviral Research - April 1, 2024 Category: Virology Authors: Jannatul Ruhan Raha Ki-Hye Kim Noopur Bhatnagar Rong Liu Chau Thuy Tien Le Bo Ryoung Park Phillip Grovenstein Surya Sekhar Pal Eun-Ju Ko Chong Hyun Shin Bao-Zhong Wang Sang-Moo Kang Source Type: research
