Identification and Characterization of Synaptic Vesicle Membrane Protein VAT-1 Homolog as a New Catechin-Binding Protein
In this study we identified synaptic vesicle membrane protein VAT-1 homolog (VAT1) as a novel EGCg-binding protein in human neuroglioma cell extracts using a magnetic pull-down assay and LC-tandem mass spectrometry. We prepared recombinant human VAT1 and analyzed its direct binding to EGCg and its alkylated derivatives using surface plasmon resonance. For EGCg and the derivative NUP-15, we measured an association constant of 0.02-0.85 ×103 M-1s-1 and a dissociation constant of nearly 8 × 10-4 s-1. The affinity Km(affinity) of their binding to VAT1 was in the 10-20 µM range and comparable with that of other EGCg-binding ...
Source: Biological and Pharmaceutical Bulletin - February 25, 2024 Category: Drugs & Pharmacology Authors: Ayaka Ikemizu Daisuke Hatta Kohei Fujimoto Mikako Honda Kaori Watanabe Kaname Ohyama Naotaka Kuroda Takashi Tanaka Keiro Shirotani Nobuhisa Iwata Source Type: research
CdAl < sub > 4 < /sub > O < sub > 7 < /sub > /CdO nanocomposites: green tea extract-mediated sol-gel auto-combustion synthesis, characterization, and study as a potential hydrogen storage material
Environ Sci Pollut Res Int. 2024 Feb 23. doi: 10.1007/s11356-024-32527-3. Online ahead of print.ABSTRACTIn this article, we present the synthesis of binary CdAl4O7/CdO nanocomposites using green tea extracts and green chemistry methods for high-performance hydrogen storage. The green tea extract contains bioactive compounds (polyphenols) that act as reducing agents, which facilitate the reaction between metal ions and water. By examining the structural and morphological characteristics of the obtained substrates using scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), and F...
Source: Environmental Science and Pollution Research International - February 23, 2024 Category: Environmental Health Authors: Meghdad Pirsaheb Hooman Seifi Elmuez A Dawi Tahereh Gholami Masoud Salavati-Niasari Source Type: research
Regulation of blue infertile flower pigmentation by WD40 transcription factor HmWDR68 in Hydrangea macrophylla 'forever summer'
CONCLUSION: These findings suggest HmWDR68 may specifically regulate blue infertile flower formation in hydrangea by enhancing delphinidin-3-O-glucoside synthesis, modulating expression of HmF3H, HmC3'5'H, HmDFR and HmANS. This study provides insights into HmWDR68's role in hydrangea's blue flowers development, offering a foundation for further research in this field.PMID:38393428 | DOI:10.1007/s11033-024-09287-x (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 23, 2024 Category: Molecular Biology Authors: Jingyi Gong Yu Wang Chao Xue Linshi Wu Song Sheng Meng Wang Jiqing Peng Shoujin Cao Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
CdAl < sub > 4 < /sub > O < sub > 7 < /sub > /CdO nanocomposites: green tea extract-mediated sol-gel auto-combustion synthesis, characterization, and study as a potential hydrogen storage material
Environ Sci Pollut Res Int. 2024 Feb 23. doi: 10.1007/s11356-024-32527-3. Online ahead of print.ABSTRACTIn this article, we present the synthesis of binary CdAl4O7/CdO nanocomposites using green tea extracts and green chemistry methods for high-performance hydrogen storage. The green tea extract contains bioactive compounds (polyphenols) that act as reducing agents, which facilitate the reaction between metal ions and water. By examining the structural and morphological characteristics of the obtained substrates using scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), and F...
Source: Environmental Science and Pollution Research International - February 23, 2024 Category: Environmental Health Authors: Meghdad Pirsaheb Hooman Seifi Elmuez A Dawi Tahereh Gholami Masoud Salavati-Niasari Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Mol Biol Cell - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Regulation of blue infertile flower pigmentation by WD40 transcription factor HmWDR68 in Hydrangea macrophylla 'forever summer'
CONCLUSION: These findings suggest HmWDR68 may specifically regulate blue infertile flower formation in hydrangea by enhancing delphinidin-3-O-glucoside synthesis, modulating expression of HmF3H, HmC3'5'H, HmDFR and HmANS. This study provides insights into HmWDR68's role in hydrangea's blue flowers development, offering a foundation for further research in this field.PMID:38393428 | DOI:10.1007/s11033-024-09287-x (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 23, 2024 Category: Molecular Biology Authors: Jingyi Gong Yu Wang Chao Xue Linshi Wu Song Sheng Meng Wang Jiqing Peng Shoujin Cao Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
