Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research
