An automated accelerated salting-out assisted solvent extraction (A-ASASE) of stilbenoids from Vitis vinifera L. branches: False proof or a proof of concept?
Talanta. 2023 Jul 22;266(Pt 1):124935. doi: 10.1016/j.talanta.2023.124935. Online ahead of print.ABSTRACTAn automated accelerated salting-out assisted solvent extraction (A-ASASE) was developed. This approach made full use of the advantages of both the accelerated solvent extraction (ASE) technique and salting-out assisted liquid-liquid extraction (SALLE), without any modifications at the level of the ASE machine. The A-ASASE combined an automated extraction at high temperature/pressure and an enrichment step using eco-friendly solvents (e.g., water and ethanol) in a fully integrated and simple way that required only low v...
Source: Talanta - July 27, 2023 Category: Chemistry Authors: Omar S Ahmed Faiza Tahanout Gilberte Marti-Mestres Jean-Fr édéric Weber Pierre Waffo-T éguo Source Type: research

An automated accelerated salting-out assisted solvent extraction (A-ASASE) of stilbenoids from Vitis vinifera L. branches: False proof or a proof of concept?
Talanta. 2023 Jul 22;266(Pt 1):124935. doi: 10.1016/j.talanta.2023.124935. Online ahead of print.ABSTRACTAn automated accelerated salting-out assisted solvent extraction (A-ASASE) was developed. This approach made full use of the advantages of both the accelerated solvent extraction (ASE) technique and salting-out assisted liquid-liquid extraction (SALLE), without any modifications at the level of the ASE machine. The A-ASASE combined an automated extraction at high temperature/pressure and an enrichment step using eco-friendly solvents (e.g., water and ethanol) in a fully integrated and simple way that required only low v...
Source: Talanta - July 27, 2023 Category: Chemistry Authors: Omar S Ahmed Faiza Tahanout Gilberte Marti-Mestres Jean-Fr édéric Weber Pierre Waffo-T éguo Source Type: research

Effect of proanthocyanidins from different sources on the digestibility, physicochemical properties and structure of gelatinized maize starch
Int J Biol Macromol. 2023 Jul 21:125935. doi: 10.1016/j.ijbiomac.2023.125935. Online ahead of print.ABSTRACTThe effect of proanthocyanidins (PAs) from Chinese bayberry leaves (BLPs), grape seeds (GSPs), peanut skins (PSPs) and pine barks (PBPs) on physicochemical properties, structure and in-vitro digestibility of gelatinized maize starch was investigated. The results showed that all PAs remarkably retarded starch digestibility, meanwhile, BLPs highlighted superiority in increasing resistant starch content from 31.29 ± 1.12 % to 68.61 ± 1.15 %. The iodine-binding affinity analysis confirmed the interaction between PAs an...
Source: International Journal of Biological Macromolecules - July 23, 2023 Category: Biochemistry Authors: Mengting Wang Haiguang Mao Zhijian Ke Rui Huang Jianchu Chen Lili Qi Jinbo Wang Source Type: research

Effect of proanthocyanidins from different sources on the digestibility, physicochemical properties and structure of gelatinized maize starch
Int J Biol Macromol. 2023 Jul 21;248:125935. doi: 10.1016/j.ijbiomac.2023.125935. Online ahead of print.ABSTRACTThe effect of proanthocyanidins (PAs) from Chinese bayberry leaves (BLPs), grape seeds (GSPs), peanut skins (PSPs) and pine barks (PBPs) on physicochemical properties, structure and in-vitro digestibility of gelatinized maize starch was investigated. The results showed that all PAs remarkably retarded starch digestibility, meanwhile, BLPs highlighted superiority in increasing resistant starch content from 31.29 ± 1.12 % to 68.61 ± 1.15 %. The iodine-binding affinity analysis confirmed the interaction between PA...
Source: International Journal of Biological Macromolecules - July 23, 2023 Category: Biochemistry Authors: Mengting Wang Haiguang Mao Zhijian Ke Rui Huang Jianchu Chen Lili Qi Jinbo Wang Source Type: research

Drug interaction potential of Ankaferd blood stopper ® in human hepatocarcinoma cells
We examined drug-metabolizing enzymes, including 7-ethoxyresorufin O-deethylase (CYP1A1), 7-methoxyresorufin O-demethylase (CYP1A2), aniline 4-hydroxylase (CYP2E1), and erythromycin N-demethylase (CYP3A4), in vitro in HepG2 cells. The expression (mRNA, protein) levels of drug-metabolizing enzymes were analyzed by qPCR and Western blotting, respectively.RESULTS: The EC05 and EC10 values for ABS were 0.37% and 0.52% (v/v), respectively. Therefore, 0.37% and 0.52% (v/v) doses were used for the remaining portion of this study. Investigation of the expression and activity levels revealed that CYP1A1-1A2, CYP2E1, and CYP3A4 acti...
Source: Turkish Journal of Medical Sciences - July 21, 2023 Category: General Medicine Authors: Asl ı Semiz Source Type: research

Drug interaction potential of Ankaferd blood stopper ® in human hepatocarcinoma cells
We examined drug-metabolizing enzymes, including 7-ethoxyresorufin O-deethylase (CYP1A1), 7-methoxyresorufin O-demethylase (CYP1A2), aniline 4-hydroxylase (CYP2E1), and erythromycin N-demethylase (CYP3A4), in vitro in HepG2 cells. The expression (mRNA, protein) levels of drug-metabolizing enzymes were analyzed by qPCR and Western blotting, respectively.RESULTS: The EC05 and EC10 values for ABS were 0.37% and 0.52% (v/v), respectively. Therefore, 0.37% and 0.52% (v/v) doses were used for the remaining portion of this study. Investigation of the expression and activity levels revealed that CYP1A1-1A2, CYP2E1, and CYP3A4 acti...
Source: Turkish Journal of Medical Sciences - July 21, 2023 Category: General Medicine Authors: Asl ı Semiz Source Type: research