The Use of Non-insulin Agents in Gestational Diabetes: Clinical Considerations in Tailoring Therapy
AbstractPurpose of ReviewTo assess evidence to date for use of non-insulin agents in treatment of gestational diabetes mellitus.Recent FindingsThere has been increasing interest in the use of non-insulin agents, primarily metformin and glyburide (which both cross the placenta). Metformin has been associated with less maternal weight gain; however, recent studies have shown a trend toward increased weight in offspring exposed to metformin in utero. Glyburide has been associated with increased neonatal hypoglycemia.SummaryGlycemic control during pregnancy is essential to optimize both maternal and fetal outcomes. There are a...
Source: Current Diabetes Reports - December 5, 2019 Category: Endocrinology Source Type: research

GLP-1 Relaxes Rat Coronary Arteries by Enhancing ATP-Sensitive Potassium Channel Currents.
Abstract GLP-1 is a new type of antidiabetic agent that possesses many beneficial effects. Although its cardiovascular actions have been widely examined, little is known about GLP-1's effects on the rat coronary artery (RCA) or about the mechanisms underpinning these effects. Here, we report that GLP-1 inhibits depolarization- or thromboxane receptor agonist (U46619)-induced RCA contraction in a dosage-dependent manner. Vasorelaxation was attenuated by denuding the endothelium, L-NAME (nitric oxide synthase inhibitor), and glyburide (KATP channel blocker) but was not affected by indomethacin (cyclooxygenas...
Source: Cardiology Research and Practice - November 29, 2019 Category: Cardiology Authors: Xiong QF, Fan SH, Li XW, Niu Y, Wang J, Zhang X, Chen YF, Shi YW, Zhang LH Tags: Cardiol Res Pract Source Type: research

Effect of Berberine on In Vitro Metabolism of Sulfonylureas: A herb-drug interactions study.
CONCLUSIONS: The metabolism of sulfonylureas and berberine was affected when these compounds were co-incubated with each other. This may be attributable to competitive binding of the herb and drug to the catalytic sites of same isozymes. PMID: 31721320 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2019 Category: Chemistry Authors: Singh A, Zhao K, Bell C, Shah AJ Tags: Rapid Commun Mass Spectrom Source Type: research

The second-generation anti-diabetic sulfonylureas inhibit Candida albicans and Candidalysin mediated activation of the NLRP3 inflammasome.
Abstract Repurposing of currently approved medications is an attractive option for development of novel treatment strategies against physiological and infectious diseases. The anti-diabetic sulfonylurea glyburide has demonstrated off-target capacity to inhibit activation of the NLRP3 inflammasome in a variety of disease models, including vaginal candidiasis caused primarily by the fungal pathogen Candida albicans Therefore, we sought to determine which of the currently approved sulfonylurea drugs prevent release of IL-1β, a major inflammasome effector, during C. albicans challenge of the human macrophage-...
Source: Antimicrobial Agents and Chemotherapy - November 10, 2019 Category: Microbiology Authors: Lowes DJ, Hevener KE, Peters BM Tags: Antimicrob Agents Chemother Source Type: research

Metformin promotes Mycobacterium tuberculosis killing and increases the production of human β-defensins in lung epithelial cells and macrophages
Publication date: Available online 2 November 2019Source: Microbes and InfectionAuthor(s): Adrian Rodriguez-Carlos, Claudia Valdez-Miramontes, Paulina Marin-Luevano, Irma González-Curiel, Jose A. Enciso-Moreno, Bruno Rivas-SantiagoAbstractDiabetes has been associated with an increased risk of developing tuberculosis. The reasons related to the increased susceptibility to develop TB in type 2 diabetes mellitus (T2DM) individuals, has not been completely elucidated. However, this susceptibility has been attributed to several factors including failures and misfunctioning of the immune system. In the present study, we aimed t...
Source: Microbes and Infection - November 2, 2019 Category: Infectious Diseases Source Type: research

Effect of Olive leaves extract on the antidiabetic effect of glyburide for possible herb-drug interaction
Publication date: Available online 11 October 2019Source: Saudi Pharmaceutical JournalAuthor(s): Maged S. Abdel-Kader, Gamal A. Soliman, Rehab F. Abdel-Rahman, Abdulaziz S. Saeedan, Reham M. Abd-Elsalam, Hanan A. OgalyAbstractThe concomitant use of olive leaves (OL) and glyburide (GLB) is a possible therapy for diabetic patients. However, there is no report about the effect of OL on the antidiabetic effect of GLB till now. In the current study, the possible interaction of olive leaves extract (OLE) with GLB was assessed to determine if there was any pharmacological benefit over GLB alone. Seven groups of male Sprague Dawle...
Source: Saudi Pharmaceutical Journal - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Mechanisms underlying the effect of an oral antihyperglycemic agent glyburide on calcium ion (Ca2+ ) movement and its related cytotoxicity in prostate cancer cells.
This study examined whether glyburide altered Ca2+ signaling and viability in PC3 human prostate cancer cells and investigated those underlying mechanisms. Intracellular Ca2+ concentrations ([Ca2+ ]i ) in suspended cells were measured by using the fluorescent Ca2+ -sensitive dye fura-2. Cell viability was examined by WST-1 assay. Glyburide at concentrations of 100-1000 μM induced [Ca2+ ]i rises. Ca2+ removal reduced the signal by approximately 60%. In Ca2+ -containing medium, glyburide-induced Ca2+ entry was inhibited by 60% by protein kinase C (PKC) activator (phorbol 12-myristate 13 acetate, PMA) and inhibitor (GF109203...
Source: Clinical and Experimental Pharmacology and Physiology - September 16, 2019 Category: Drugs & Pharmacology Authors: Sun GC, Liang WZ, Jan CR Tags: Clin Exp Pharmacol Physiol Source Type: research

Magnetic Resonance Imaging Pilot Study of Intravenous Glyburide in Traumatic Brain Injury
Journal of Neurotrauma, Ahead of Print. (Source: Journal of Neurotrauma)
Source: Journal of Neurotrauma - August 26, 2019 Category: Neurology Authors: Howard M. Eisenberg Martha E. Shenton Ofer Pasternak J. Marc Simard David O. Okonkwo Christina Aldrich Feng He Sonia Jain Erik G. Hayman Source Type: research

NLRP3 inflammasome inhibitor glyburide expedites diabetic-induced impaired fracture healing.
Abstract Localized inflammation is accompanied by the diabetic-induced fracture. The present study aims to investigate the therapeutic effects of glyburide, an NLRP3 inflammasome inhibitor, in a diabetic-induced fracture model. An animal model of diabetic-induced fracture was established and the mice were administrated with metformin or glyburide for 3 weeks. Quantitative polymerase chain reaction (qPCR) and Western blotting were used to evaluate the relative expressions of IFN-γ, TNF-α, and IL-6. Micro-computed tomography (μCT) scanning was applied to evaluate bone callus formation. Histopathology exam...
Source: Immunobiology - August 22, 2019 Category: Allergy & Immunology Authors: Yang X, Qu C, Jia J, Zhan Y Tags: Immunobiology Source Type: research

Inflammasome inhibition blocks cardiac glycoside cell toxicity [Molecular Bases of Disease]
We report that digoxin and related compounds activate the NLRP3 inflammasome in macrophages and cardiomyocytes at concentrations achievable during clinical use. Inflammasome activation initiates the maturation and release of the inflammatory cytokine IL-1β and the programmed cell death pathway pyroptosis in a caspase-1–dependent manner. Notably, the same fluxes of potassium and calcium cations that affect heart contraction also induce inflammasome activation in human but not murine cells. Pharmaceuticals that antagonize these fluxes, including glyburide and verapamil, also inhibit inflammasome activation by cardiac glyc...
Source: Journal of Biological Chemistry - August 22, 2019 Category: Chemistry Authors: Doris L. LaRock, Jenna S. Sands, Ethan Ettouati, Marine Richard, Paul J. Bushway, Eric D. Adler, Victor Nizet, Christopher N. LaRock Tags: Immunology Source Type: research

Calcification biomarkers and vascular dysfunction in obesity and type 2 diabetes: influence of oral hypoglycemic agents.
In conclusion, obese patients with MUO/T2D have elevated circulating OPN, OPG and RANTES; in these patients, antidiabetic treatment reduces only circulating OPG. Further study is needed to better understand the mechanisms of vascular calcifications in obesity and diabetes. PMID: 31408377 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - August 12, 2019 Category: Endocrinology Authors: Schinzari F, Tesauro M, Bertoli A, Valentini A, Veneziani A, Cambia U, Cardillo C Tags: Am J Physiol Endocrinol Metab Source Type: research

Pharmacodynamic interaction of cumin seeds (Cuminum cyminum L.) with glyburide in diabetes
Authors: Khurana, Ginpreet Kaur / Upadhyay, Nidhi / Tharappel, Leo J Philip / Invally, Mihir (Source: Journal of Complementary and Integrative Medicine)
Source: Journal of Complementary and Integrative Medicine - July 25, 2019 Category: Complementary Medicine Source Type: research

Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells
Here a membrane ‐coating approach to display large transmembrane protein receptors to the surface of nanoparticles for drug delivery to the brain is developed and validated. The study also establishes a new formulation of glyburide that can be translated into clinical applications to improve clinical management of brain injuries. AbstractCell membrane coating has recently emerged as a promising biomimetic approach to engineering nanoparticles (NPs) for targeted drug delivery. However, simple cell membrane coating may not meet the need for efficient drug delivery to the brain. Here, a novel molecular engineering strategy ...
Source: Small - July 9, 2019 Category: Nanotechnology Authors: Junning Ma, Shenqi Zhang, Jun Liu, Fuyao Liu, Fenyi Du, Miao Li, Ann T. Chen, Youmei Bao, Hee Won Suh, Jonathan Avery, Gang Deng, Yu Zhou, Peng Wu, Kevin Sheth, Haijun Wang, Jiangbing Zhou Tags: Full Paper Source Type: research

Glyburide, a NLRP3 inhibitor, decreases inflammatory response and is a candidate to reduce pathology in Leishmania braziliensis infection
Cutaneous leishmaniasis (CL) caused by Leishmania braziliensis is characterized by an exaggerated inflammatory response that leads to parasite control, but is also the cause of tissue damage and ulcer formation (Carvalho et al., 2012). The NLRP3 Inflammasome is an intracellular protein complex which is activated by pathogen associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs), promoting activation of caspase 1 and release of active IL-1 β (Latz et al., 2013). In C57BL/6 mice infected with a nonhealing L. (Source: Journal of Investigative Dermatology)
Source: Journal of Investigative Dermatology - June 24, 2019 Category: Dermatology Authors: Augusto M. Carvalho, Fernanda O. Novais, Camilla S. Paix ão, Camila I. de Oliveira, Paulo Roberto Lima Machado, Lucas P. Carvalho, Phillip Scott, Edgar M. Carvalho Tags: Letters to the Editor Source Type: research