Duchenne muscular dystrophy progression induced by downhill running is accompanied by increased endomysial fibrosis and oxidative damage DNA in muscle of mdx mice
This study aims to understand the relationship between myogenic regulation, inflammation and oxidative stress parameters, and disease progression induced by downhill running in the skeletal muscle of an experimental model of DMD. Six-month-old C57BL/10 and C57BL/10-DMDmdx male mice were distributed into three groups: Control (C),mdx, andmdx + Exercise (mdx + Ex). Animals were trained in a downhill running protocol for seven weeks. The gastrocnemius muscle was subjected to histopathology, muscle regeneration (myoD and myogenin), inflammation (COX-2), oxidative stress (8-OHdG) immunohistochemistry markers, and gene e...
Source: Journal of Molecular Histology - November 8, 2022 Category: Laboratory Medicine Source Type: research

Structural and functional studies of a snake venom phospholipase A < sub > 2 < /sub > -like protein complexed to an inhibitor purified from Tabernaemontana catharinensis
Biochimie. 2022 Oct 20:S0300-9084(22)00277-2. doi: 10.1016/j.biochi.2022.10.011. Online ahead of print.ABSTRACTSnake envenomation is an ongoing global health problem and tropical neglected disease that afflicts millions of people each year. The only specific treatment, antivenom, has several limitations that affects its proper distribution to the victims and its efficacy against local effects, such as myonecrosis. The main responsible for this consequence are the phospholipases A2 (PLA2) and PLA2-like proteins, such as BthTX-I from Bothrops jararacussu. Folk medicine resorts to plants such as Tabernaemontana catharinensis ...
Source: Biochimie - October 23, 2022 Category: Biochemistry Authors: Rafael J Borges F ábio F Cardoso Cicilia de Carvalho Ivan de Marino Paulo S Pereira Andreimar M Soares Maeli Dal-Pai-Silva Isabel Us ón Marcos R M Fontes Source Type: research

Structural and functional studies of a snake venom phospholipase A < sub > 2 < /sub > -like protein complexed to an inhibitor purified from Tabernaemontana catharinensis
Biochimie. 2022 Oct 20:S0300-9084(22)00277-2. doi: 10.1016/j.biochi.2022.10.011. Online ahead of print.ABSTRACTSnake envenomation is an ongoing global health problem and tropical neglected disease that afflicts millions of people each year. The only specific treatment, antivenom, has several limitations that affects its proper distribution to the victims and its efficacy against local effects, such as myonecrosis. The main responsible for this consequence are the phospholipases A2 (PLA2) and PLA2-like proteins, such as BthTX-I from Bothrops jararacussu. Folk medicine resorts to plants such as Tabernaemontana catharinensis ...
Source: Biochimie - October 23, 2022 Category: Biochemistry Authors: Rafael J Borges F ábio F Cardoso Cicilia de Carvalho Ivan de Marino Paulo S Pereira Andreimar M Soares Maeli Dal-Pai-Silva Isabel Us ón Marcos R M Fontes Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

Association between tissue human neutrophil peptide 1-3 levels and cardiovascular phenotype: a prospective, longitudinal cohort study
CONCLUSION: HNP1-3 tissue deposition is positively associated with the severity of CAD, myonecrosis, and long-term sequelae. HNP1-3 levels may be suppressed using colchicine.PMID:36268757 | DOI:10.1177/03000605221127099 (Source: Atherosclerosis)
Source: Atherosclerosis - October 21, 2022 Category: Cardiology Authors: Rami Abu Fanne Yaron Arbel Ehud Chorin Emad Maraga Gabriel M Groisman Abd Alroof Higazi Shmuel Banai Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

Association between tissue human neutrophil peptide 1-3 levels and cardiovascular phenotype: a prospective, longitudinal cohort study
CONCLUSION: HNP1-3 tissue deposition is positively associated with the severity of CAD, myonecrosis, and long-term sequelae. HNP1-3 levels may be suppressed using colchicine.PMID:36268757 | DOI:10.1177/03000605221127099 (Source: Atherosclerosis)
Source: Atherosclerosis - October 21, 2022 Category: Cardiology Authors: Rami Abu Fanne Yaron Arbel Ehud Chorin Emad Maraga Gabriel M Groisman Abd Alroof Higazi Shmuel Banai Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research

The location of protein oxidation in dystrophic skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy
This study used the dystrophic mdx mouse model for DMD to visualise the precise location of different oxidative modifications to proteins in dystrophic muscles, including both reversible (protein thiol oxidation and s-nitrosylation) and irreversible (carbonylation and dityrosine formation) oxidation at various stages of dystrophic muscle necrosis and regeneration. High levels of protein oxidation were observed in mdx myofibres undergoing degeneration and immune cell infiltration (myonecrosis). Since irreversible protein oxidation, especially dityrosine formation, was only colocalised to areas of myonecrosis, we suggest tha...
Source: Acta Histochemica - October 21, 2022 Category: Biochemistry Authors: Tomohito Iwasaki Jessica R Terrill Kei Kawarai Yusei Miyata Takayoshi Tagami Naoyuki Maeda Yasuhiro Hasegawa Takafumi Watanabe Miranda D Grounds Peter G Arthur Source Type: research