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Increasing incidence of contact-lens-related < em > Acanthamoeba < /em > keratitis in a tertiary ophthalmology department in an Italian population
CONCLUSION: Although PCR is the most common method used, the increased incidence of AK could mainly be related to a proper IVCM interpretation. A broad-spectrum antibiotic, such as levofloxacin might play a role in the early treatment of AK reducing the virulence of the amoeba.PMID:38509758 | DOI:10.1177/11206721241242165 (Source: European Journal of Ophthalmology)
Source: European Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Marco Messina Davide Tucci Stefano Mocini Virginia Marruso Silvia Crotti Dalia Said Harminder Singh Dua Carlo Cagini Source Type: research

Adaptation to an amoeba host drives selection of virulence-associated traits and genetic variation in saprotrophic Candida albicans
Amoebae are micropredators that play an important role in controlling fungal populations in ecosystems. However, the interaction between fungi and their amoebic predators suggests that the pressure from predatory selection can significantly influence the development of fungal virulence and evolutionary processes. Thus, the purpose of this study was to investigate the adaptation of saprotrophic Candida albicans strains during their interactions with Acanthamoeba castellanii. We conducted a comprehensive analysis of survival after co-culture by colony counting of the yeast cells and examining yeast cell phenotypic and geneti...
Source: Frontiers in cellular and infection microbiology - March 13, 2024 Category: Microbiology Source Type: research

Metagenomics insights into microbiome and antibiotic resistance genes from free living amoeba in chlorinated wastewater effluents
In this study we comprehensively analyzed the microbiome, antibiotic resistome and virulence factors (VFs) within FLA isolated from final treated effluents of two wastewater treatment plants (WWTPs) using shotgun metagenomics. Acanthamoeba has been identified as the most common FLA, followed by Entamoeba. The bacterial diversity showed no significant difference (p > 0.05) in FLA microbiomes obtained from the two WWTPs. At phylum level, the most dominant taxa were Proteobacteria, followed by Firmicutes and Actinobacteria. The most abundant genera identified were Enterobacter followed by Citrobacter, Paenibacillus, and Cu...
Source: International Journal of Hygiene and Environmental health - March 12, 2024 Category: Environmental Health Authors: Thobela Conco-Biyela Muneer Ahmad Malla Oluyemi Olatunji Awolusi Mushal Allam Arshad Ismail Thor A Stenstr öm Faizal Bux Sheena Kumari Source Type: research

Acanthamoeba Infection and Nasal Rinsing, United States, 1994 –2022
J. C. Haston et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - March 12, 2024 Category: Infectious Diseases Source Type: research

Differentiation of acanthamoeba keratitis from other non-acanthamoeba keratitis: Risk factors and clinical features
by Shaker Osaywid Alreshidi, Jos é Manuel Vargas, Khabir Ahmad, Ahmed Yousef Alothman, Eman D. Albalawi, Abdulmohsen Almulhim, Saad Hamdan Alenezi, Hani Basher ALBalawi, Naif Mamdouh Alali, Faris Hashem, Mohanna Aljindan IntroductionInfectious Keratitis is one of the most common ocular emergencies seen by ophthalmologists. Our aim is to identify the risk factors and clinical features of Acanthamoeba Keratitis (AK). MethodsThis retrospective chart review study was conducted at King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia, and included all the microbial keratitis cases, male and female patients of all ages. The...
Source: PLoS One - March 12, 2024 Category: Biomedical Science Authors: Shaker Osaywid Alreshidi Source Type: research

Are Thermotolerant and Osmotolerant Characteristics of < em > Acanthamoeba < /em > Species an Indicator of Pathogenicity?
CONCLUSION: According to our research results, we believe that tolerance to 39 °C and 1 M mannitol is not an indicator of pathogenicity. More studies with Acanthamoeba strains are required to clarify this issue.PMID:38449362 | DOI:10.4274/tpd.galenos.2024.92408 (Source: Turkish Society for Parasitology)
Source: Turkish Society for Parasitology - March 7, 2024 Category: Parasitology Authors: Merve Kahraman Z übeyda Akın Polat Source Type: research