Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran
J Water Health. 2023 Oct;21(10):1572-1579. doi: 10.2166/wh.2023.203.ABSTRACTDifferent species of free-living amoeba (FLA) have been abundantly isolated in harsh environmental conditions such as hot springs and brackish water. The present study aimed to isolate, genotype, and evaluate the pathogenicity of FLAs in Qom Roud, a large river, in the centre of Iran. About 500 mL of water samples (n = 30) were collected from each sampling site and were investigated for the presence of FLAs using morphological and molecular characters. Genotype identification was performed using DNA sequencing and a phylogenetic tree was constructe...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Azam Pourabbasi Ardekan Maryam Niyyati Reza Saberi Leyli Zanjirani Farahani Marziye Fatemi Source Type: research

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2017-2022
J Water Health. 2023 Oct;21(10):1421-1447. doi: 10.2166/wh.2023.094.ABSTRACTThe current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. In total, 416 outbreaks were attributed to the waterborne transmission of parasitic protozoa. Cryptosporidium accounted for 77.4% (322) of outbreaks, while Giardia was identified as the etiological agent in 17.1% (71). Toxoplasma gondii and Naegleria fowleri were the primary causes in 1.4% (6) and 1% (4) of outbreaks, respectively. Blastocystis hominis, Cyclospora cayetanensis, and Dientamoeba fragilis were indepen...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Pavlina Bourli Aida Vafae Eslahi Ourania Tzoraki Panagiotis Karanis Source Type: research

First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil
The objective of this work was to identify FLA present in sewage treatment plants in Porto Alegre, Brazil. About 1 L samples were collected from eight stations (raw and treated sewage) in January, February, July, and August 2022. The samples were sown in monoxenic culture, and the isolated amoebae were subjected to morphological and molecular identification. Polymerase chain reaction results indicated the presence of the genus Acanthamoeba in 100% of the samples. Gene sequencing showed the presence of Acanthamoeba lenticulata and Acanthamoeba polyphaga - T5 and T4 genotypes - respectively, which are related to pathogenicit...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Thaisla Cristiane Borella da Silva Daniel Leal Dos Santos Marilise Brittes Rott Source Type: research

Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran
J Water Health. 2023 Oct;21(10):1572-1579. doi: 10.2166/wh.2023.203.ABSTRACTDifferent species of free-living amoeba (FLA) have been abundantly isolated in harsh environmental conditions such as hot springs and brackish water. The present study aimed to isolate, genotype, and evaluate the pathogenicity of FLAs in Qom Roud, a large river, in the centre of Iran. About 500 mL of water samples (n = 30) were collected from each sampling site and were investigated for the presence of FLAs using morphological and molecular characters. Genotype identification was performed using DNA sequencing and a phylogenetic tree was constructe...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Azam Pourabbasi Ardekan Maryam Niyyati Reza Saberi Leyli Zanjirani Farahani Marziye Fatemi Source Type: research

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2017-2022
J Water Health. 2023 Oct;21(10):1421-1447. doi: 10.2166/wh.2023.094.ABSTRACTThe current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. In total, 416 outbreaks were attributed to the waterborne transmission of parasitic protozoa. Cryptosporidium accounted for 77.4% (322) of outbreaks, while Giardia was identified as the etiological agent in 17.1% (71). Toxoplasma gondii and Naegleria fowleri were the primary causes in 1.4% (6) and 1% (4) of outbreaks, respectively. Blastocystis hominis, Cyclospora cayetanensis, and Dientamoeba fragilis were indepen...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Pavlina Bourli Aida Vafae Eslahi Ourania Tzoraki Panagiotis Karanis Source Type: research

First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil
The objective of this work was to identify FLA present in sewage treatment plants in Porto Alegre, Brazil. About 1 L samples were collected from eight stations (raw and treated sewage) in January, February, July, and August 2022. The samples were sown in monoxenic culture, and the isolated amoebae were subjected to morphological and molecular identification. Polymerase chain reaction results indicated the presence of the genus Acanthamoeba in 100% of the samples. Gene sequencing showed the presence of Acanthamoeba lenticulata and Acanthamoeba polyphaga - T5 and T4 genotypes - respectively, which are related to pathogenicit...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Thaisla Cristiane Borella da Silva Daniel Leal Dos Santos Marilise Brittes Rott Source Type: research

Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran
J Water Health. 2023 Oct;21(10):1572-1579. doi: 10.2166/wh.2023.203.ABSTRACTDifferent species of free-living amoeba (FLA) have been abundantly isolated in harsh environmental conditions such as hot springs and brackish water. The present study aimed to isolate, genotype, and evaluate the pathogenicity of FLAs in Qom Roud, a large river, in the centre of Iran. About 500 mL of water samples (n = 30) were collected from each sampling site and were investigated for the presence of FLAs using morphological and molecular characters. Genotype identification was performed using DNA sequencing and a phylogenetic tree was constructe...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Azam Pourabbasi Ardekan Maryam Niyyati Reza Saberi Leyli Zanjirani Farahani Marziye Fatemi Source Type: research

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2017-2022
J Water Health. 2023 Oct;21(10):1421-1447. doi: 10.2166/wh.2023.094.ABSTRACTThe current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. In total, 416 outbreaks were attributed to the waterborne transmission of parasitic protozoa. Cryptosporidium accounted for 77.4% (322) of outbreaks, while Giardia was identified as the etiological agent in 17.1% (71). Toxoplasma gondii and Naegleria fowleri were the primary causes in 1.4% (6) and 1% (4) of outbreaks, respectively. Blastocystis hominis, Cyclospora cayetanensis, and Dientamoeba fragilis were indepen...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Pavlina Bourli Aida Vafae Eslahi Ourania Tzoraki Panagiotis Karanis Source Type: research

First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil
The objective of this work was to identify FLA present in sewage treatment plants in Porto Alegre, Brazil. About 1 L samples were collected from eight stations (raw and treated sewage) in January, February, July, and August 2022. The samples were sown in monoxenic culture, and the isolated amoebae were subjected to morphological and molecular identification. Polymerase chain reaction results indicated the presence of the genus Acanthamoeba in 100% of the samples. Gene sequencing showed the presence of Acanthamoeba lenticulata and Acanthamoeba polyphaga - T5 and T4 genotypes - respectively, which are related to pathogenicit...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Thaisla Cristiane Borella da Silva Daniel Leal Dos Santos Marilise Brittes Rott Source Type: research

Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran
J Water Health. 2023 Oct;21(10):1572-1579. doi: 10.2166/wh.2023.203.ABSTRACTDifferent species of free-living amoeba (FLA) have been abundantly isolated in harsh environmental conditions such as hot springs and brackish water. The present study aimed to isolate, genotype, and evaluate the pathogenicity of FLAs in Qom Roud, a large river, in the centre of Iran. About 500 mL of water samples (n = 30) were collected from each sampling site and were investigated for the presence of FLAs using morphological and molecular characters. Genotype identification was performed using DNA sequencing and a phylogenetic tree was constructe...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Azam Pourabbasi Ardekan Maryam Niyyati Reza Saberi Leyli Zanjirani Farahani Marziye Fatemi Source Type: research

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2017-2022
J Water Health. 2023 Oct;21(10):1421-1447. doi: 10.2166/wh.2023.094.ABSTRACTThe current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. In total, 416 outbreaks were attributed to the waterborne transmission of parasitic protozoa. Cryptosporidium accounted for 77.4% (322) of outbreaks, while Giardia was identified as the etiological agent in 17.1% (71). Toxoplasma gondii and Naegleria fowleri were the primary causes in 1.4% (6) and 1% (4) of outbreaks, respectively. Blastocystis hominis, Cyclospora cayetanensis, and Dientamoeba fragilis were indepen...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Pavlina Bourli Aida Vafae Eslahi Ourania Tzoraki Panagiotis Karanis Source Type: research

First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil
The objective of this work was to identify FLA present in sewage treatment plants in Porto Alegre, Brazil. About 1 L samples were collected from eight stations (raw and treated sewage) in January, February, July, and August 2022. The samples were sown in monoxenic culture, and the isolated amoebae were subjected to morphological and molecular identification. Polymerase chain reaction results indicated the presence of the genus Acanthamoeba in 100% of the samples. Gene sequencing showed the presence of Acanthamoeba lenticulata and Acanthamoeba polyphaga - T5 and T4 genotypes - respectively, which are related to pathogenicit...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Thaisla Cristiane Borella da Silva Daniel Leal Dos Santos Marilise Brittes Rott Source Type: research

Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran
J Water Health. 2023 Oct;21(10):1572-1579. doi: 10.2166/wh.2023.203.ABSTRACTDifferent species of free-living amoeba (FLA) have been abundantly isolated in harsh environmental conditions such as hot springs and brackish water. The present study aimed to isolate, genotype, and evaluate the pathogenicity of FLAs in Qom Roud, a large river, in the centre of Iran. About 500 mL of water samples (n = 30) were collected from each sampling site and were investigated for the presence of FLAs using morphological and molecular characters. Genotype identification was performed using DNA sequencing and a phylogenetic tree was constructe...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Azam Pourabbasi Ardekan Maryam Niyyati Reza Saberi Leyli Zanjirani Farahani Marziye Fatemi Source Type: research

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks - an update 2017-2022
J Water Health. 2023 Oct;21(10):1421-1447. doi: 10.2166/wh.2023.094.ABSTRACTThe current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. In total, 416 outbreaks were attributed to the waterborne transmission of parasitic protozoa. Cryptosporidium accounted for 77.4% (322) of outbreaks, while Giardia was identified as the etiological agent in 17.1% (71). Toxoplasma gondii and Naegleria fowleri were the primary causes in 1.4% (6) and 1% (4) of outbreaks, respectively. Blastocystis hominis, Cyclospora cayetanensis, and Dientamoeba fragilis were indepen...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Pavlina Bourli Aida Vafae Eslahi Ourania Tzoraki Panagiotis Karanis Source Type: research

First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil
The objective of this work was to identify FLA present in sewage treatment plants in Porto Alegre, Brazil. About 1 L samples were collected from eight stations (raw and treated sewage) in January, February, July, and August 2022. The samples were sown in monoxenic culture, and the isolated amoebae were subjected to morphological and molecular identification. Polymerase chain reaction results indicated the presence of the genus Acanthamoeba in 100% of the samples. Gene sequencing showed the presence of Acanthamoeba lenticulata and Acanthamoeba polyphaga - T5 and T4 genotypes - respectively, which are related to pathogenicit...
Source: Journal of Water and Health - October 30, 2023 Category: Environmental Health Authors: Thaisla Cristiane Borella da Silva Daniel Leal Dos Santos Marilise Brittes Rott Source Type: research