Free Living Amoebozoa Infection Research This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Case Report: A Series of Three Meningoencephalitis Cases Caused by Acanthamoeba spp. from Eastern India
We present three cases of Acanthamoeba meningoencephalitis in immunocompetent individuals from Eastern India. The first patient presented with fever and headache; the second with headache, visual disturbance, and squint; and the third presented in a drowsy state. The cases presented on March 3, 18, and 21, 2023 respectively. The first two patients had concomitant tubercular meningitis for which they received antitubercular therapy and steroid. Their cerebrospinal fluid showed slight lymphocytic pleocytosis and increased protein. The diagnosis was done by microscopy, culture, and polymerase chain reaction. They received a c...
Source: The American Journal of Tropical Medicine and Hygiene - January 8, 2024 Category: Tropical Medicine Authors: Soumendra Nath Haldar Kokila Banerjee Dolanchampa Modak Agnibho Mondal Chayan Sharma Teja Vasireddy Rucha Kalyan Karad Hardik Bharatbhai Patel Debajyoti Majumdar Boudhayan Bhattacharjee Sumeeta Khurana Tapashi Ghosh Subhasish Kamal Guha Bibhuti Saha Source Type: research

The role of naturally acquired intracellular < i > Pseudomonas aeruginosa < /i > in the development of < i > Acanthamoeba < /i > keratitis in an animal model
This study assessed the presence of intracellular bacteria inAcanthamoeba species recovered from domestic tap water and corneas of two different AK patients and examined the impact of naturally occurring intracellular bacteria withinAcanthamoeba on the severity of corneal infections in rats. Methodology/Principal findingsHousehold water and corneal swabs were collected from AK patients.Acanthamoeba strains and genotypes were confirmed by sequencing.Acanthamoeba isolates were assessed for the presence of intracellular bacteria using sequencing, fluorescencein situ hybridization (FISH), and electron microscopy. The viability...
Source: PLoS Neglected Tropical Diseases - January 2, 2024 Category: Tropical Medicine Authors: Binod Rayamajhee Source Type: research

Human parasites in surface water used for recreation in Salta, Argentina
In this study, two recreational aquatic environments were studied in the province of Salta, Argentina. Water samples collected from three different locations, two from a creek and one from the outlet of a thermal complex, were monitored at four time points. Physicochemical and microbiological characterization of each point was conducted, as well as a search for parasites and amebae. Parasites were identified through optical microscopy observations and free-living amebae (FLA) were isolated by spiking in Petri dishes followed by subsequent molecular identification. Water samples from the outlet of the thermal complex showed...
Source: Revista Argentina de Microbiologia - December 28, 2023 Category: Microbiology Authors: Diego G Sanguino-Jorquera Hugo R Poma Ver ónica B Rajal Mar ía M Juárez Ver ónica P Irazusta Source Type: research

Characterization of Rosculus vilicus sp. nov., a rhizarian amoeba interacting with Mycobacterium avium subsp. paratuberculosis
In conclusion, we characterized a new environmental amoeba species at the cellular and genome level that was able to interact with Mycobacterium avium subsp. paratuberculosis. As a result, R. vilicus is a potential candidate as environmental reservoir for Mycobacterium avium subsp. paratuberculosis but further experiments are needed to test this hypothesis. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - December 22, 2023 Category: Microbiology Source Type: research

Corneal ring infiltrate- far more than Acanthamoeba keratitis: review of pathophysiology, morphology, differential diagnosis and management
ConclusionCorneal ring infiltrate might be triggered not only by ocular infectious and non-infectious factors, but also by systemic conditions. Clinical assessment is crucial for empirical diagnosis. Furthermore, treatment is targeted towards the underlying condition but should begin with anti-infectious regimen until not proven otherwise. (Source: Journal of Ophthalmic Inflammation and Infection)
Source: Journal of Ophthalmic Inflammation and Infection - December 19, 2023 Category: Opthalmology Source Type: research

A brief history of metal recruitment in protozoan predation
Trends Microbiol. 2023 Dec 15:S0966-842X(23)00326-8. doi: 10.1016/j.tim.2023.11.008. Online ahead of print.ABSTRACTMetals and metalloids are used as weapons for predatory feeding by unicellular eukaryotes on prokaryotes. This review emphasizes the role of metal(loid) bioavailability over the course of Earth's history, coupled with eukaryogenesis and the evolution of the mitochondrion to trace the emergence and use of the metal(loid) prey-killing phagosome as a feeding strategy. Members of the genera Acanthamoeba and Dictyostelium use metals such as zinc (Zn) and copper (Cu), and possibly metalloids, to kill their bacterial...
Source: Trends in Microbiology - December 16, 2023 Category: Microbiology Authors: Yanshuang Yu Yuan-Ping Li Kexin Ren Xiuli Hao Ernest Chi Fru Regin R ønn Windell L Rivera Karsten Becker Renwei Feng Jun Yang Christopher Rensing Source Type: research

Incidence of Acanthamoeba Keratitis in the United Kingdom in 2015: A Prospective National Survey
CONCLUSIONS: This study provides the first published data on the nationwide incidence of AK in the United Kingdom. The findings confirm an increasing incidence of AK, particularly among contact lens wearers since 1997/8 to 1998/9. PRSCLs were identified as a significant risk factor compared with DDSCLs. Misdiagnosis and treatment delays remain an ongoing problem for patients with AK.PMID:38097200 | DOI:10.1097/ICO.0000000000003445 (Source: Herpes)
Source: Herpes - December 14, 2023 Category: Infectious Diseases Authors: Haneen Jasim Mariusz Grzeda Barny Foot Derek Tole Jeremy J Hoffman Source Type: research

Proteases of Acanthamoeba
Parasitol Res. 2023 Dec 8;123(1):19. doi: 10.1007/s00436-023-08059-z.ABSTRACTThe members of genus Acanthamoeba are the etiological agent of uncommon but severe or even fatal opportunistic infections in human beings. The presence of different classes of intracellular and extracellular proteases including serine proteases, cysteine proteases, and metalloproteases has been well documented in environmental and clinical isolates of Acanthamoeba spp. However, the role of the proteolytic enzymes in physiological, biological, and pathological mechanisms of the amoeba remains partially investigated. Some attempts have been conducte...
Source: Cell Research - December 8, 2023 Category: Cytology Authors: Behroz Mahdavi Poor Jalil Rashedi Vahid Asgharzadeh Amirali Mirmazhary Nazila Gheitarani Source Type: research