White matter microstructure in transmasculine and cisgender adolescents: A multiparametric and multivariate study
by Lindsey T. Thurston, Malvina N. Skorska, Nancy J. Lobaugh, Kenneth J. Zucker, M. Mallar Chakravarty, Meng-Chuan Lai, Sofia Chavez, Doug P. VanderLaan
Adolescence is a sensitive developmental period for neural sex/gender differentiation. The present study used multiparametric mapping to better characterize adolescent white matter (WM) microstructure. WM microstructure was investigated using diffusion tensor indices (fractional anisotropy; mean, radial, and axial diffusivity [AD]) and quantitative T1 relaxometry (T1) in hormone therapy naïve adolescent cisgender girls, cisgender boys, and transgender boys (i.e., assigne...
Source: PLoS One - March 12, 2024 Category: Biomedical Science Authors: Lindsey T. Thurston Source Type: research
Kinetic Characterization of Estradiol Glucuronidation by Liver Microsomes and Expressed UGT Enzymes: The Effects of Organic Solvents
ConclusionOrganic solvents such as methanol, ethanol, and acetonitrile showed great potential in adjusting the glucuronidation of estradiol. DMSO is the most suitable solvent due to its minimal influence on estradiol glucuronidation. Researchers should be cautious in selecting appropriate solvents to get accurate results when assessing the metabolism of a new chemical entity. (Source: European Journal of Drug Metabolism and Pharmacokinetics)
Source: European Journal of Drug Metabolism and Pharmacokinetics - March 12, 2024 Category: Drugs & Pharmacology Source Type: research
Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals
Biomater Res. 2024 Mar 7;28:0010. doi: 10.34133/bmr.0010. eCollection 2024.ABSTRACTThe increasing prevalence of endocrine-disrupting chemicals (EDCs) in our environment is a growing concern, with numerous studies highlighting their adverse effects on the human endocrine system. Among the EDCs, estrogenic endocrine-disrupting chemicals (eEDCs) are exogenous compounds that perturb estrogenic hormone function by interfering with estrogen receptor (ER) homo (α/α, β/β) or hetero (α/β) dimerization. To date, a comprehensive screening approach for eEDCs affecting all ER dimer forms in live cells is lacking. Here, we develop...
Source: Cell Research - March 11, 2024 Category: Cytology Authors: Gyuho Choi Hyunkoo Kang Jung-Soo Suh Haksoo Lee Kiseok Han Gaeun Yoo Hyejin Jo Yeong Min Shin Tae-Jin Kim BuHyun Youn Source Type: research
Combined effects of binary mixtures of 17 β-estradiol and testosterone in western mosquitofish (Gambusia affinis) after full life-cycle exposure
In this study, the endocrine disrupting effects of binary mixtures of 17β-estradiol (E2) and testosterone (T) in western mosquitofish (Gambusia affinis) were assessed by analyzing the sex ratio, secondary sex characteristics, gonadal histology, and transcriptional expression of target genes related to the hypothalamic-pituitary-gonadal (HPG) axis in G. affinis (from embryos) continuously exposed to E2 (50 ng/L), T (T1: 50 ng/L; T2: 200 ng/L), and mixtures of both (E2 + T1: 50 + 50 ng/L; E2 + T2: 50 + 200 ng/L) for 119 d. The results showed that exposure to E2 + T1 and E2 + T2 reduced the length ratio of ray 4/6 ratio in m...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 9, 2024 Category: Biochemistry Authors: Chen-Si Wang Gui-Zhen Fang Xiao-Pei Li Guo-Yong Huang Xie Lingtian Guang-Guo Ying Source Type: research
Combined effects of binary mixtures of 17 β-estradiol and testosterone in western mosquitofish (Gambusia affinis) after full life-cycle exposure
In this study, the endocrine disrupting effects of binary mixtures of 17β-estradiol (E2) and testosterone (T) in western mosquitofish (Gambusia affinis) were assessed by analyzing the sex ratio, secondary sex characteristics, gonadal histology, and transcriptional expression of target genes related to the hypothalamic-pituitary-gonadal (HPG) axis in G. affinis (from embryos) continuously exposed to E2 (50 ng/L), T (T1: 50 ng/L; T2: 200 ng/L), and mixtures of both (E2 + T1: 50 + 50 ng/L; E2 + T2: 50 + 200 ng/L) for 119 d. The results showed that exposure to E2 + T1 and E2 + T2 reduced the length ratio of ray 4/6 ratio in m...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 9, 2024 Category: Biochemistry Authors: Chen-Si Wang Gui-Zhen Fang Xiao-Pei Li Guo-Yong Huang Lingtian Xie Guang-Guo Ying Source Type: research
Combined effects of binary mixtures of 17 β-estradiol and testosterone in western mosquitofish (Gambusia affinis) after full life-cycle exposure
In this study, the endocrine disrupting effects of binary mixtures of 17β-estradiol (E2) and testosterone (T) in western mosquitofish (Gambusia affinis) were assessed by analyzing the sex ratio, secondary sex characteristics, gonadal histology, and transcriptional expression of target genes related to the hypothalamic-pituitary-gonadal (HPG) axis in G. affinis (from embryos) continuously exposed to E2 (50 ng/L), T (T1: 50 ng/L; T2: 200 ng/L), and mixtures of both (E2 + T1: 50 + 50 ng/L; E2 + T2: 50 + 200 ng/L) for 119 d. The results showed that exposure to E2 + T1 and E2 + T2 reduced the length ratio of ray 4/6 ratio in m...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 9, 2024 Category: Biochemistry Authors: Chen-Si Wang Gui-Zhen Fang Xiao-Pei Li Guo-Yong Huang Lingtian Xie Guang-Guo Ying Source Type: research
Combined effects of binary mixtures of 17 β-estradiol and testosterone in western mosquitofish (Gambusia affinis) after full life-cycle exposure
In this study, the endocrine disrupting effects of binary mixtures of 17β-estradiol (E2) and testosterone (T) in western mosquitofish (Gambusia affinis) were assessed by analyzing the sex ratio, secondary sex characteristics, gonadal histology, and transcriptional expression of target genes related to the hypothalamic-pituitary-gonadal (HPG) axis in G. affinis (from embryos) continuously exposed to E2 (50 ng/L), T (T1: 50 ng/L; T2: 200 ng/L), and mixtures of both (E2 + T1: 50 + 50 ng/L; E2 + T2: 50 + 200 ng/L) for 119 d. The results showed that exposure to E2 + T1 and E2 + T2 reduced the length ratio of ray 4/6 ratio in m...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 9, 2024 Category: Biochemistry Authors: Chen-Si Wang Gui-Zhen Fang Xiao-Pei Li Guo-Yong Huang Lingtian Xie Guang-Guo Ying Source Type: research
Adipokine secretion and lipolysis following gender-affirming treatment in transgender individuals
ConclusionIn TW, gonadal hormone suppression results in transient changes in cytokines and in TM there are some changes in NA-stimulated lipolysis following testosterone treatment. However, despite the known metabolic effects of sex hormones, the overall effects of GAHT on adipose tissue function are small and likely have limited clinical relevance, but larger studies with longer follow-up are needed to confirm these findings.Trial registrationClinicalTrials.gov Identifier: NCT02518009, Retrospectively registered 7 August 2015. (Source: Journal of Endocrinological Investigation)
Source: Journal of Endocrinological Investigation - March 9, 2024 Category: Endocrinology Source Type: research
AKR1C3 in carcinomas: from multifaceted roles to therapeutic strategies
Aldo-Keto Reductase Family 1 Member C3 (AKR1C3), also known as type 5 17β-hydroxysteroid dehydrogenase (17β-HSD5) or prostaglandin F (PGF) synthase, functions as a pivotal enzyme in androgen biosynthesis. It catalyzes the conversion of weak androgens, estrone (a weak estrogen), and PGD2 into potent androgens (testosterone and 5α-dihydrotestosterone), 17β-estradiol (a potent estrogen), and 11β-PGF2α, respectively. Elevated levels of AKR1C3 activate androgen receptor (AR) signaling pathway, contributing to tumor recurrence and imparting resistance to cancer therapies. The overexpression of AKR1C3 serves as an oncogenic...
Source: Frontiers in Pharmacology - March 8, 2024 Category: Drugs & Pharmacology Source Type: research
Phthalate metabolites and sex steroid hormones in relation to obesity in US adults: NHANES 2013-2016
ConclusionsIndividuals with obesity exhibit lower levels of TT and SHBG, accompanied by elevated estradiol levels. Exposure to PAEs disrupts sex hormone levels, contributing to an increased risk of obesity in US adults. In the exploration of the interrelationships among these three factors, the RF and XGBoost algorithm models demonstrated superior performance, with sex hormones displaying higher variable importance. (Source: Frontiers in Endocrinology)
Source: Frontiers in Endocrinology - March 8, 2024 Category: Endocrinology Source Type: research
Per- and polyfluoroalkyl substances inhibit human and rat 17 β-hydroxysteroid dehydrogenase 1: Quantitative structure-activity relationship and molecular docking analysis
In conclusion, PFAS exhibit inhibitory effects on human and rat 17β-HSD1 depending on factors such as carbon chain length, degree of fluorination, and the presence of carboxylic acid or sulfonic acid groups, with a notable V-shaped shift observed at C11.PMID:38452703 | DOI:10.1016/j.ecoenv.2024.116173 (Source: Ecotoxicology and Environmental Safety)
Source: Ecotoxicology and Environmental Safety - March 7, 2024 Category: Environmental Health Authors: Chao Wen Huan Chen Yunbing Tang Hang Lin Congcong Xu Yingfen Ying Yang Zhu Xinjun Miao Ren-Shan Ge Chao Chen Shangqin Chen Source Type: research
Letrozole induced a polycystic ovary syndrome model in zebrafish by interfering with the hypothalamic-pituitary-gonadal axis
This study compared the traits of zebrafish with three diagnostic criteria for human PCOS, and the diagnostic criteria for zebrafish PCOS were proposed: decreased fecundity, elevated testosterone (T) or 11-ketotestosterone (11-KT) levels and increased cortical-alveolar oocyte (CO) ratio, enhancing the zebrafish PCOS model's accuracy. According to the mammalian PCOS classification, the type of zebrafsh PCOS is divided into four phenotypes (A, B, C and D), but the four phenotypes of zebrafish PCOS are not fully covered in the existing studies (A and D). In this study, we successfully induced phenotype B zebrafish PCOS model ...
Source: Environmental Pollution - March 7, 2024 Category: Environmental Health Authors: Fucong Zhang Chen Tang Jingyi Wang Tingting Lin Wei Ge Chengyong He Chunyan Yang Zhenghong Zuo Source Type: research
Per- and polyfluoroalkyl substances inhibit human and rat 17 β-hydroxysteroid dehydrogenase 1: Quantitative structure-activity relationship and molecular docking analysis
In conclusion, PFAS exhibit inhibitory effects on human and rat 17β-HSD1 depending on factors such as carbon chain length, degree of fluorination, and the presence of carboxylic acid or sulfonic acid groups, with a notable V-shaped shift observed at C11.PMID:38452703 | DOI:10.1016/j.ecoenv.2024.116173 (Source: Ecotoxicology and Environmental Safety)
Source: Ecotoxicology and Environmental Safety - March 7, 2024 Category: Environmental Health Authors: Chao Wen Huan Chen Yunbing Tang Hang Lin Congcong Xu Yingfen Ying Yang Zhu Xinjun Miao Ren-Shan Ge Chao Chen Shangqin Chen Source Type: research
