BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research
BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research
BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research
BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research
Mitochondrial extracellular vesicles, autoimmunity and myocarditis
For many decades viral infections have been suspected as ‘triggers’ of autoimmune disease, but mechanisms for how this could occur have been difficult to establish. Recent studies have shown that viral infections that are commonly associated with viral myocarditis and other autoimmune diseases such as coxsackievirus B3 (CVB3) and SARS-CoV-2 target mitochondria and are released from cells in mitochondrial vesicles that are able to activate the innate immune response. Studies have shown that Toll-like receptor (TLR)4 and the inflammasome pathway are activated by mitochondrial components. Autoreactivity against cardiac my...
Source: Frontiers in Immunology - March 14, 2024 Category: Allergy & Immunology Source Type: research
Familial Dilated Cardiomyopathy: A Novel MED9 Short Isoform Identification
Int J Mol Sci. 2024 Mar 6;25(5):3057. doi: 10.3390/ijms25053057.ABSTRACTFamilial dilated cardiomyopathy (DCM) is among the leading indications for heart transplantation. DCM alters the transcriptomic profile. The alteration or activation/silencing of physiologically operating transcripts may explain the onset and progression of this pathological state. The mediator complex (MED) plays a fundamental role in the transcription process. The aim of this study is to investigate the MED subunits, which are altered in DCM, to identify target crossroads genes. RNA sequencing allowed us to identify specific MED subunits that are alt...
Source: Atherosclerosis - March 13, 2024 Category: Cardiology Authors: Monica Franzese Mario Zanfardino Andrea Soricelli Annapaola Coppola Ciro Maiello Marco Salvatore Concetta Schiano Claudio Napoli Source Type: research
Familial Dilated Cardiomyopathy: A Novel MED9 Short Isoform Identification
Int J Mol Sci. 2024 Mar 6;25(5):3057. doi: 10.3390/ijms25053057.ABSTRACTFamilial dilated cardiomyopathy (DCM) is among the leading indications for heart transplantation. DCM alters the transcriptomic profile. The alteration or activation/silencing of physiologically operating transcripts may explain the onset and progression of this pathological state. The mediator complex (MED) plays a fundamental role in the transcription process. The aim of this study is to investigate the MED subunits, which are altered in DCM, to identify target crossroads genes. RNA sequencing allowed us to identify specific MED subunits that are alt...
Source: Molecular Medicine - March 13, 2024 Category: Molecular Biology Authors: Monica Franzese Mario Zanfardino Andrea Soricelli Annapaola Coppola Ciro Maiello Marco Salvatore Concetta Schiano Claudio Napoli Source Type: research
Generation of a TBX20 homozygous knockout stem cell line (WAe009-A-1E) by episomal vector-based CRISPR/Cas9 system
Stem Cell Res. 2024 Mar 11;77:103384. doi: 10.1016/j.scr.2024.103384. Online ahead of print.ABSTRACTThe T-box family transcription factor gene TBX20 plays a crucial role in cardiac development and function. TBX20 mutations are associated with congenital heart disease, dilated cardiomyopathy, arrhythmias, and heart failure. To further study the role of TBX20 in human heart, here we generated a homozygous TBX20 knockout (TBX20-KO) human embryonic stem cell line using the CRISPR/Cas9 system. This TBX20-KO cell line maintains normal morphology, pluripotency, and karyotype, making it a valuable tool for investigating TBX20's ro...
Source: Cell Research - March 13, 2024 Category: Cytology Authors: Xiaojie Hou Junsheng Mu Source Type: research
Familial Dilated Cardiomyopathy: A Novel MED9 Short Isoform Identification
Int J Mol Sci. 2024 Mar 6;25(5):3057. doi: 10.3390/ijms25053057.ABSTRACTFamilial dilated cardiomyopathy (DCM) is among the leading indications for heart transplantation. DCM alters the transcriptomic profile. The alteration or activation/silencing of physiologically operating transcripts may explain the onset and progression of this pathological state. The mediator complex (MED) plays a fundamental role in the transcription process. The aim of this study is to investigate the MED subunits, which are altered in DCM, to identify target crossroads genes. RNA sequencing allowed us to identify specific MED subunits that are alt...
Source: Atherosclerosis - March 13, 2024 Category: Cardiology Authors: Monica Franzese Mario Zanfardino Andrea Soricelli Annapaola Coppola Ciro Maiello Marco Salvatore Concetta Schiano Claudio Napoli Source Type: research
Beyond Conduction Impairment: Unveiling the Profound Myocardial Injury in Left Bundle Branch Block
Left bundle branch block (LBBB) represents a frequently encountered conduction system disorder. Despite its widespread occurrence, a continual dilemma persists regarding its intricate association with underlying cardiomyopathy and its pivotal role in the initiation of dilated cardiomyopathy. The pathological alterations linked to LBBB-induced cardiomyopathy (LBBB-CM) have remained elusive. (Source: Heart Rhythm)
Source: Heart Rhythm - March 13, 2024 Category: Cardiology Authors: Xiaoxian Wang, Beibei Ge, Changqing Miao, Christopher Lee, Jorge E. Romero, Peng Li, Fang Wang, Di Xu, Minglong Chen, Dianfu Li, Dong Li, Mingxia Li, Fang Xu, Yan Li, Chanjuan Gong, Cynthia C. Taub, Jing Yao Source Type: research
Immediate pharmacotherapy intensification after cardiac resynchronization therapy: incidence, characteristics, and impact
ConclusionsImmediate intensification of HF medication was achieved in 54% of CRT recipients and was significantly higher in patients without excessive QRS prolongation, preserved renal function, and dilated cardiomyopathy than others. In patients with LBBB morphology and QRS ≥ 120 ms, IPI was associated with a significantly better prognosis and fewer HF hospitalizations after CRT implantation than others. (Source: ESC Heart Failure)
Source: ESC Heart Failure - March 12, 2024 Category: Cardiology Authors: Kojiro Ogawa,
Hiro Yamasaki,
Kazutaka Aonuma,
Masafumi Otani,
Ai Hattori,
Masako Baba,
Kentaro Yoshida,
Miyako Igarashi,
Hidetaka Nishina,
Kou Suzuki,
Akihiko Nogami,
Masaki Ieda Tags: Original Article Source Type: research
Clinical and Cardiac MRI characteristics: Prognosis in Patients with Alcoholic cardiomyopathy
Alcoholic cardiomyopathy (ACM) is recognized as a type of non-ischemic dilated cardiomyopathy(DCM). To date, the clinical prognosis of ACM remains a topic of debate in previous studies and there are limited studies on its cardiac MRI characteristics. The aim of this study was to summarize the clinical and MRI features of ACM patients, and to identify the predictors of adverse prognosis based on clinical characteristics and MRI imaging findings. (Source: Clinical Radiology)
Source: Clinical Radiology - March 12, 2024 Category: Radiology Authors: Wenchuan Wang, Shuang Li, Baiyan Zhuang, Hui Wang, Yue Ren, Lei Xu Source Type: research
A specific inflammatory suppression fibroblast subpopulation characterized by MHCII expression in human dilated cardiomyopathy
In conclusion, the present study identified a novel subpopulation of fibroblasts that specifically expresses MHCII-encoding genes. This specific apFBs can suppress the inflammation occurring in DCM. Our findings further elucidate the composition of the fibro-inflammatory microenvironment in DCM, and provide a novel therapeutic target.PMID:38462549 | DOI:10.1007/s11010-024-04939-9 (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - March 11, 2024 Category: Biochemistry Authors: Xi Fan Kai Huang Yuming Wu Sheng Jin Liewen Pang Yiqing Wang Bo Jin Xiaotian Sun Source Type: research
Seroprevalence of Chagas disease at the Instituto Nacional de Cardiolog ía Ignacio Chávez from 2004 to 2010
CONCLUSION: In conclusion, complete blockage of the right branch of the bundle of His (44.2%) is one of the primary conduction disorders in the patients studied. Regarding seroprevalence, 14% of patients diagnosed with dilated cardiomyopathy had anti-T. cruzi antibodies.PMID:38467078 | DOI:10.24875/ACM.23000181 (Source: Archivos de Cardiologia de Mexico)
Source: Archivos de Cardiologia de Mexico - March 11, 2024 Category: Cardiology Authors: Diana V Res éndiz-Solís Alejandro Ruiz-S ánchez Martha A Ballinas-Verdugo Source Type: research
A specific inflammatory suppression fibroblast subpopulation characterized by MHCII expression in human dilated cardiomyopathy
In conclusion, the present study identified a novel subpopulation of fibroblasts that specifically expresses MHCII-encoding genes. This specific apFBs can suppress the inflammation occurring in DCM. Our findings further elucidate the composition of the fibro-inflammatory microenvironment in DCM, and provide a novel therapeutic target.PMID:38462549 | DOI:10.1007/s11010-024-04939-9 (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - March 11, 2024 Category: Biochemistry Authors: Xi Fan Kai Huang Yuming Wu Sheng Jin Liewen Pang Yiqing Wang Bo Jin Xiaotian Sun Source Type: research
