Dynamic headspace GC-MS/MS analysis of ethylene oxide and 2-chloroethanol in dry food commodities: a novel approach
This study, for the first time, aimed at establishing a dynamic headspace-GC-MS/MS method for the simultaneous determination of these two analytes in acetonitrile extracts of cumin, ashwagandha, chilli powder, turmeric powder, guar gum, locust bean gum, and ginger powder. The samples (4 g) were extracted using acetonitrile (10 mL). A dispersive-solid phase extraction cleanup step with primary secondary amine sorbent (50 mg/mL) reduced the interfering signal of (matrix-derived) acetaldehyde by >40% in chilli powder, ginger, turmeric, and guar gum. This cleanup was not required for sesame seeds. With high selectivity and ...
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - October 9, 2023 Category: Environmental Health Authors: Apurva Nerpagar Nagnath Langade Reshma Patil Sanket Chiplunkar Jitendra Kelkar Kaushik Banerjee Source Type: research

Dynamic headspace GC-MS/MS analysis of ethylene oxide and 2-chloroethanol in dry food commodities: a novel approach
This study, for the first time, aimed at establishing a dynamic headspace-GC-MS/MS method for the simultaneous determination of these two analytes in acetonitrile extracts of cumin, ashwagandha, chilli powder, turmeric powder, guar gum, locust bean gum, and ginger powder. The samples (4 g) were extracted using acetonitrile (10 mL). A dispersive-solid phase extraction cleanup step with primary secondary amine sorbent (50 mg/mL) reduced the interfering signal of (matrix-derived) acetaldehyde by >40% in chilli powder, ginger, turmeric, and guar gum. This cleanup was not required for sesame seeds. With high selectivity and ...
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - October 9, 2023 Category: Environmental Health Authors: Apurva Nerpagar Nagnath Langade Reshma Patil Sanket Chiplunkar Jitendra Kelkar Kaushik Banerjee Source Type: research

Genes, Vol. 14, Pages 1730: Thymoquinone Potentially Modulates the Expression of Key Onco- and Tumor Suppressor miRNAs in Prostate and Colon Cancer Cell Lines: Insights from PC3 and HCT-15 Cells
This study aimed to investigate the effects of TQ on the expression profile of key tumor suppressor and onco-suppressor miRNAs in PC3 prostate cancer cells and HCT-15 colon cancer cells. Cell viability assays revealed that TQ inhibited the growth of both cell lines in a dose-dependent manner, with IC50 values of approximately 82.59 μM for HCT-15 and 55.83 μM for PC3 cells. Following TQ treatment at the IC50 concentrations, miRNA expression analysis demonstrated that TQ significantly downregulated miR-21-5p expression in HCT-15 cells and upregulated miR-34a-5p, miR-221-5p, miR-17-5p, and miR-21-5p expr...
Source: Genes - August 30, 2023 Category: Genetics & Stem Cells Authors: Sof ía Madeline Osorio-Pérez Carolina Estrada-Meza Luis M. Ruiz-Manriquez Mar ía Goretti Arvizu-Espinosa Aashish Srivastava Ashutosh Sharma Sujay Paul Tags: Brief Report Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

The interaction impact of compost and biostimulants on growth, yield and oil content of black cumin (Nigella sativa L.) plants
This study was conducted during the seasons 2020/2021 and 2021/2022 to investigate the effect of the interaction between compost and biostimulants on growth measurements, seed yield, and oil production of black cumin (Nigella sativa L.). Four levels of fertilizer (0.6, 12, 18 tons/ha) were used. While the biostimulants were ascorbic acid (AS) at 100 ppm, yeast extract (YE) at 8 g/L, and AS at 100 ppm + 8 g/L YE. The plants were treated with these stimuli as follows: control (without addition), 100 ppm, YE at 8 g/L, and the results showed that fertilization at all levels, as well as foliar spraying with the used stimuli, le...
Source: Braz J Biol - August 23, 2023 Category: Biology Authors: A H Al-Fraihat A A Zatimeh H H Alhrout S Y Al-Dalain M Mostipan Source Type: research

Therapeutic and Phytochemical Properties of Thymoquinone Derived from Nigella Sativa
CONCLUSION: TQ seems to have a variety of consequences on how infected cells behave at the cellular level.PMID:37605475 | DOI:10.2174/2589977515666230811092410 (Source: Cell Research)
Source: Cell Research - August 22, 2023 Category: Cytology Authors: Gaurav Tiwari Monisha Gupta Lalchand D Devhare Ruchi Tiwari Source Type: research

Black cumin ethanolic extract reduce aortic intima media thickness due to cigarette smoke exposure in experimental rats
Background and Aims: Cigarette smoking could induce pro-atherogenic changes seen in endothelial cells and increase inflammation markers due to oxidative stress. This can lead to increased intima media thickness of blood vessel and accelerate atherosclerosis. Black cumin (Nigella sativa) may inhibit oxidative stress. Aim of this study is to explore the link between cigarette smoking exposure to endothelial-nitric oxide synthase and vascular cell adhesion molecule-1 as well as the protective effects of black cumin in reducing aortic intima media thickness caused by cigarette smoking. (Source: Atherosclerosis)
Source: Atherosclerosis - August 1, 2023 Category: Cardiology Authors: R.A. Nugraha, B.P. Khrisna, T. Putra, L.G. Rinjani, M. Ardiana, I.G. Suryawan, B. Pikir Source Type: research

Using days alive and out of hospital to measure surgical outcomes in New Zealand: a cross-sectional study
Conclusion In New Zealand after direct risk adjustment, differences in DAOH90 between three types of surgical procedure reflected their respective risk levels and associated mortality rates. Outlier DHBs were identified for each procedure. Thus, our approach to analysing DAOH90 appears to have considerable face validity and potential utility for contributing to the measurement of perioperative outcomes in an audit or quality improvement setting. (Source: BMJ Open)
Source: BMJ Open - July 25, 2023 Category: General Medicine Authors: Boyle, L., Lumley, T., Cumin, D., Campbell, D., Merry, A. F. Tags: Open access, Surgery Source Type: research

Activity of cumin essential oil to control fascioliasis: Efficacy and changes in the tegument of Fasciola hepatica
In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tes...
Source: Experimental Parasitology - July 16, 2023 Category: Parasitology Authors: Alexia Brauner de Mello Bruna Baccega Filipe Obelar Martins Ivandra Ign ês de Santi Yan Wahast Islab ão Marjorie de Giacometi Mauro Pereira Soares Nara Am élia da Rosa Farias Camila Belmonte de Oliveira Source Type: research

Activity of cumin essential oil to control fascioliasis: Efficacy and changes in the tegument of Fasciola hepatica
In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tes...
Source: Experimental Parasitology - July 16, 2023 Category: Parasitology Authors: Alexia Brauner de Mello Bruna Baccega Filipe Obelar Martins Ivandra Ign ês de Santi Yan Wahast Islab ão Marjorie de Giacometi Mauro Pereira Soares Nara Am élia da Rosa Farias Camila Belmonte de Oliveira Source Type: research

Activity of cumin essential oil to control fascioliasis: Efficacy and changes in the tegument of Fasciola hepatica
In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tes...
Source: Experimental Parasitology - July 16, 2023 Category: Parasitology Authors: Alexia Brauner de Mello Bruna Baccega Filipe Obelar Martins Ivandra Ign ês de Santi Yan Wahast Islab ão Marjorie de Giacometi Mauro Pereira Soares Nara Am élia da Rosa Farias Camila Belmonte de Oliveira Source Type: research