Development of a non-infectious control for viral hemorrhagic fever PCR assays
by Matthew A. Knox, Collette Bromhead, David TS Hayman Assay validation is an essential component of disease surveillance testing, but can be problematic in settings where access to positive control material is limited and a safety risk for handlers. Here we describe a single non-infectious synthetic control that can help develop and validate the PCR based detection of the viral causes of Crimean-Congo hemorrhagic fever, Ebola virus disease, Lassa fever, Marburg virus disease and Rift Valley fever. We designed non-infectious synthetic DNA oligonucleotide sequences incorporating primer binding sites suitable for five assay...
Source: PLoS Neglected Tropical Diseases - April 22, 2024 Category: Tropical Medicine Authors: Matthew A. Knox Source Type: research

Crimean-Congo Hemorrhagic Fever Virus for Clinicians —Epidemiology, Clinical Manifestations, and Prevention
M. G. Frank et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 19, 2024 Category: Infectious Diseases Source Type: research

Crimean-Congo Hemorrhagic Fever Virus for Clinicians —Diagnosis, Clinical Management, and Therapeutics
M. G. Frank et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 18, 2024 Category: Infectious Diseases Source Type: research

Epidemiologic Survey of Crimean-Congo Hemorrhagic Fever Virus in Suids, Spain
M. Fr ías et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 16, 2024 Category: Infectious Diseases Source Type: research

Crimean Congo Hemorrhagic Fever Virus for Clinicians —Virology, Pathogenesis, and Pathology
M. G. Frank et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 15, 2024 Category: Infectious Diseases Source Type: research

Crimean-Congo Hemorrhagic Fever Virus in Ticks Collected from Cattle, Corsica, France, 2023
P. Kiwan et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 11, 2024 Category: Infectious Diseases Source Type: research

A retrospective study of Crimean-Congo hemorrhagic fever in Iraq
Conclusion: Strict precautions and precise surveillance should be implemented to control the disease and protect the communityby improving the diagnosis and treatment of CCHF. The authors recommend another future study to detect the genotypingand sequencing of CCHFV in Iraq.Keywords: Crimean –Congo hemorrhagic fever; Iraq; tick. (Source: African Health Sciences)
Source: African Health Sciences - April 1, 2024 Category: African Health Authors: Doaa Adnan Shaker, Muna Tawfeeq Abd, Nawar Jassim Alsalih, Sinan Ghazi Mahdi, Mohenned Alsaadawi, Ihab Raqeeb Aakef, Tareq Jafaar Aljandeel Source Type: research

Case Management of Imported Crimean-Congo Hemorrhagic Fever, Senegal, July 2023
We report an imported Crimean-Congo hemorrhagic fever case in Senegal. The patient received PCR confirmation of virus infection 10 days after symptom onset. We identified 46 patient contacts in Senegal; 87.7% were healthcare professionals. Strengthening border crossing and community surveillance systems can help reduce the risks of infectious disease transmission.PMID:38526304 | DOI:10.3201/eid3004.231492 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Youssou Bamar Gueye Yoro Sall Jerlie Loko Roka Ibra Diagne Kalidou Djibril Sow Alseyni Diallo Pape Samba Di èye Jean Pierre Diallo Boly Diop Omer Pasi Source Type: research

Crimean-Congo Hemorrhagic Fever Virus Seroprevalence in Human and Livestock Populations, Northern Tanzania
Emerg Infect Dis. 2024 Apr;30(4):836-838. doi: 10.3201/eid3004.231204.ABSTRACTWe conducted a cross-sectional study of Crimean-Congo hemorrhagic fever virus (CCHFV) in northern Tanzania. CCHFV seroprevalence in humans and ruminant livestock was high, as were spatial heterogeneity levels. CCHFV could represent an unrecognized human health risk in this region and should be included as a differential diagnosis for febrile illness.PMID:38526202 | DOI:10.3201/eid3004.231204 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Ellen C Hughes William de Glanville Tito Kibona Blandina Theophil Mmbaga Melinda K Rostal Emanuel S Swai Sarah Cleaveland Felix Lankester Brian J Willett Kathryn J Allan Source Type: research

Crimean-Congo Hemorrhagic Fever Virus Diversity and Reassortment, Pakistan, 2017-2020
Emerg Infect Dis. 2024 Apr;30(4):654-664. doi: 10.3201/eid3004.231155.ABSTRACTSporadic cases and outbreaks of Crimean-Congo hemorrhagic fever (CCHF) have been documented across Pakistan since 1976; however, data regarding the diversity of CCHF virus (CCHFV) in Pakistan is sparse. We whole-genome sequenced 36 CCHFV samples collected from persons infected in Pakistan during 2017-2020. Most CCHF cases were from Rawalpindi (n = 10), followed by Peshawar (n = 7) and Islamabad (n = 4). Phylogenetic analysis revealed the Asia-1 genotype was dominant, but 4 reassorted strains were identified. Strains with reassorted medium gene se...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Massab Umair Zaira Rehman Shannon Whitmer Melissa Mobley Ammad Fahim Aamer Ikram Muhammad Salman Joel M Montgomery John D Klena Source Type: research

Animal Exposure Model for Mapping Crimean-Congo Hemorrhagic Fever Virus Emergence Risk
Emerg Infect Dis. 2024 Apr;30(4):672-680. doi: 10.3201/eid3004.221604.ABSTRACTTo estimate the determinants of spatial variation in Crimean-Congo hemorrhagic fever virus (CCHFV) transmission and to create a risk map as a preventive public health tool, we designed a survey of small domestic ruminants in Andalusia, Spain. To assess CCHFV exposure spatial distribution, we analyzed serum from 2,440 sheep and goats by using a double-antigen ELISA and modeled exposure probability with environmental predictors by using generalized linear mixed models. CCHFV antibodies detected in 84 samples confirmed low CCHFV prevalence in small ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Sara Baz-Flores D ébora Jiménez-Martín Alfonso Peralbo-Moreno Cesar Herraiz David Cano-Terriza Ra úl Cuadrado-Matías Ignacio Garc ía-Bocanegra Francisco Ruiz-Fons Source Type: research

Case Management of Imported Crimean-Congo Hemorrhagic Fever, Senegal, July 2023
We report an imported Crimean-Congo hemorrhagic fever case in Senegal. The patient received PCR confirmation of virus infection 10 days after symptom onset. We identified 46 patient contacts in Senegal; 87.7% were healthcare professionals. Strengthening border crossing and community surveillance systems can help reduce the risks of infectious disease transmission.PMID:38526304 | DOI:10.3201/eid3004.231492 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Youssou Bamar Gueye Yoro Sall Jerlie Loko Roka Ibra Diagne Kalidou Djibril Sow Alseyni Diallo Pape Samba Di èye Jean Pierre Diallo Boly Diop Omer Pasi Source Type: research

Crimean-Congo Hemorrhagic Fever Virus Seroprevalence in Human and Livestock Populations, Northern Tanzania
Emerg Infect Dis. 2024 Apr;30(4):836-838. doi: 10.3201/eid3004.231204.ABSTRACTWe conducted a cross-sectional study of Crimean-Congo hemorrhagic fever virus (CCHFV) in northern Tanzania. CCHFV seroprevalence in humans and ruminant livestock was high, as were spatial heterogeneity levels. CCHFV could represent an unrecognized human health risk in this region and should be included as a differential diagnosis for febrile illness.PMID:38526202 | DOI:10.3201/eid3004.231204 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Ellen C Hughes William de Glanville Tito Kibona Blandina Theophil Mmbaga Melinda K Rostal Emanuel S Swai Sarah Cleaveland Felix Lankester Brian J Willett Kathryn J Allan Source Type: research

Crimean-Congo Hemorrhagic Fever Virus Diversity and Reassortment, Pakistan, 2017-2020
Emerg Infect Dis. 2024 Apr;30(4):654-664. doi: 10.3201/eid3004.231155.ABSTRACTSporadic cases and outbreaks of Crimean-Congo hemorrhagic fever (CCHF) have been documented across Pakistan since 1976; however, data regarding the diversity of CCHF virus (CCHFV) in Pakistan is sparse. We whole-genome sequenced 36 CCHFV samples collected from persons infected in Pakistan during 2017-2020. Most CCHF cases were from Rawalpindi (n = 10), followed by Peshawar (n = 7) and Islamabad (n = 4). Phylogenetic analysis revealed the Asia-1 genotype was dominant, but 4 reassorted strains were identified. Strains with reassorted medium gene se...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Massab Umair Zaira Rehman Shannon Whitmer Melissa Mobley Ammad Fahim Aamer Ikram Muhammad Salman Joel M Montgomery John D Klena Source Type: research

Animal Exposure Model for Mapping Crimean-Congo Hemorrhagic Fever Virus Emergence Risk
Emerg Infect Dis. 2024 Apr;30(4):672-680. doi: 10.3201/eid3004.221604.ABSTRACTTo estimate the determinants of spatial variation in Crimean-Congo hemorrhagic fever virus (CCHFV) transmission and to create a risk map as a preventive public health tool, we designed a survey of small domestic ruminants in Andalusia, Spain. To assess CCHFV exposure spatial distribution, we analyzed serum from 2,440 sheep and goats by using a double-antigen ELISA and modeled exposure probability with environmental predictors by using generalized linear mixed models. CCHFV antibodies detected in 84 samples confirmed low CCHFV prevalence in small ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Sara Baz-Flores D ébora Jiménez-Martín Alfonso Peralbo-Moreno Cesar Herraiz David Cano-Terriza Ra úl Cuadrado-Matías Ignacio Garc ía-Bocanegra Francisco Ruiz-Fons Source Type: research