Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat
Genomics and genome editing promise enormous opportunities for crop improvement and elementary research. Precise modification in the specific targeted location of a genome has profited over the unplanned insertional events which are generally accomplished employing unadventurous means of genetic modifications. The advent of new genome editing procedures viz; zinc finger nucleases (ZFNs), homing endonucleases, transcription activator like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enable molecular scientists to modulate gene expressions or create novel genes with high precision and efficiency....
Source: Frontiers in Genetics - April 18, 2023 Category: Genetics & Stem Cells Source Type: research
Molecules, Vol. 28, Pages 3402: Selenized Chickpea Sprouts Hydrolysates as a Potential Anti-Aging Ingredient
-Ricardo
Skin aging represents a health and aesthetic problem that could result in infections and skin diseases. Bioactive peptides can potentially be used in skin aging regulation. Chickpea (Cicer arietinum L.) selenoproteins were obtained from germination with 2 mg Na2SeO3/100 g of seeds for 2 days. Alcalase, pepsin, and trypsin were used as hydrolyzers, and a membrane < 10 kDa was used to fractionate the hydrolysate. Se content, antioxidant capacity, elastase and collagen inhibition, functional stability, and preventative capacity were analyzed. Significant increases in Se content were found in germinated...
Source: Molecules - April 12, 2023 Category: Chemistry Authors: Sayra N. Serrano-Sandoval Antonio Jim énez-Rodríguez Jes ús Hernández-Pérez Rocio Alejandra Chavez-Santoscoy Daniela Guardado-F élix Marilena Antunes-Ricardo Tags: Article Source Type: research
Evaluating the efficacy of non-thermal microbial load reduction treatments of heat labile food components for in vitro fermentation experiments
In this study, liquid chemical sterilant alternatives to moist heat sterilization were explored for treating pulse flours for use during in vitro simulated colon fermentation. Key attributes considered in chemical selection were accessibility, impact on treated food componen ts, and effectiveness of the treatments for reducing microbial load. Three chemicals were selected for evaluation, bleach, alcohol, and hydrogen peroxide, at varying concentrations. Flours chosen for testing were from green lentil, field pea, chickpea, or sprouted green lentil. All treatments signif icantly reduced microbial loads, though there were st...
Source: PLoS One - March 21, 2023 Category: Biomedical Science Authors: Andrew Paff Source Type: research
Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum
Gene. 2023 Mar 16:147372. doi: 10.1016/j.gene.2023.147372. Online ahead of print.ABSTRACTOrf147, a cytotoxic peptide, has been found to cause cytoplasmic male sterility (CMS) in Cajanus cajanifolius (pigeonpea). In our study, Orf147 was introduced into self-pollinating Cicer arietinum (chickpea) using Agrobacterium-mediated transformation for induction of CMS. The stable integration and expression of the transgene has been assessed through PCR and qRT-PCR analysis. In addition, phenotypic sterility analysis has been performed, considering developmental parameters like flower development, pod formation and flower drop. Tran...
Source: Gene - March 18, 2023 Category: Genetics & Stem Cells Authors: Joorie Bhattacharya Dumbala Srinivas Reddy Kalyani Prasad Rahul B Nitnavare Pooja Bhatnagar-Mathur Palakolanu Sudhakar Reddy Source Type: research
Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum
Gene. 2023 Mar 16:147372. doi: 10.1016/j.gene.2023.147372. Online ahead of print.ABSTRACTOrf147, a cytotoxic peptide, has been found to cause cytoplasmic male sterility (CMS) in Cajanus cajanifolius (pigeonpea). In our study, Orf147 was introduced into self-pollinating Cicer arietinum (chickpea) using Agrobacterium-mediated transformation for induction of CMS. The stable integration and expression of the transgene has been assessed through PCR and qRT-PCR analysis. In addition, phenotypic sterility analysis has been performed, considering developmental parameters like flower development, pod formation and flower drop. Tran...
Source: Gene - March 18, 2023 Category: Genetics & Stem Cells Authors: Joorie Bhattacharya Dumbala Srinivas Reddy Kalyani Prasad Rahul B Nitnavare Pooja Bhatnagar-Mathur Palakolanu Sudhakar Reddy Source Type: research
Estimation of genetic diversity using seed storage protein (SSP) profiling in wild and cultivated species of Cicer L
CONCLUSION: Our results indicate that SDS-PAGE of SSPs is a powerful technique in determining the genetic diversity plus it is easily adaptable, due to its cost effectiveness in comparison to other genomics tools.PMID:36894768 | DOI:10.1007/s11033-023-08358-9 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - March 9, 2023 Category: Molecular Biology Authors: Uswah Khalid Muhammad Qandeel Waheed Najma Parveen Mian Abdur Rehman Arif Anjuman Arif Source Type: research
Enhanced secretion of satiety-promoting gut hormones in healthy humans after consumption of white bread enriched with cellular chickpea flour: A randomized crossover study
CONCLUSIONS: The novel use of intact chickpea cells to replace refined flours in a white bread stimulates an anorexigenic gut hormone response and has potential to improve dietary strategies for prevention and treatment of cardiometabolic diseases. This study was registered at clinicaltrials.gov as NCT03994276.PMID:36811474 | DOI:10.1016/j.ajcnut.2022.12.008 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 22, 2023 Category: Nutrition Authors: Balazs H Bajka Ana M Pinto Natalia Perez-Moral Shikha Saha Peter Ryden Jennifer Ahn-Jarvis Alice van der Schoot Catherine Bland Sarah E Berry Peter R Ellis Cathrina H Edwards Source Type: research
Enhanced secretion of satiety-promoting gut hormones in healthy humans after consumption of white bread enriched with cellular chickpea flour: A randomized crossover study
CONCLUSIONS: The novel use of intact chickpea cells to replace refined flours in a white bread stimulates an anorexigenic gut hormone response and has potential to improve dietary strategies for prevention and treatment of cardiometabolic diseases. This study was registered at clinicaltrials.gov as NCT03994276.PMID:36811474 | DOI:10.1016/j.ajcnut.2022.12.008 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 22, 2023 Category: Nutrition Authors: Balazs H Bajka Ana M Pinto Natalia Perez-Moral Shikha Saha Peter Ryden Jennifer Ahn-Jarvis Alice van der Schoot Catherine Bland Sarah E Berry Peter R Ellis Cathrina H Edwards Source Type: research
Genome-wide identification of the fibrillin gene family in chickpea (Cicer arietinum L.) and its response to drought stress
In this study, we identified 12 potential CaFBNs that are ranging from 25 kDa-42.92 kDa and are mostly basic. These proteins were hydrophilic in nature and generally resided in the chloroplast. The CaFBN genes were located on different chromosomes like 1, 4, 5, and 7. All FBNs shared conserved motifs and possessed a higher number of stress-responsive elements. For evolutionary analysis, a phylogenetic tree of CaFBNs with other plants' FBNs was constructed and clustered into 11 FBN subgroups. For expression analysis, 21 day old chickpea seedling was exposed to dehydration stress by withholding water. We also performed vario...
Source: International Journal of Biological Macromolecules - February 22, 2023 Category: Biochemistry Authors: Anuradha Pandey Punam Sharma Divya Mishra Sharmistha Dey Rinku Malviya Dipak Gayen Source Type: research
Enhanced secretion of satiety-promoting gut hormones in healthy humans after consumption of white bread enriched with cellular chickpea flour: A randomized crossover study
CONCLUSIONS: The novel use of intact chickpea cells to replace refined flours in a white bread stimulates an anorexigenic gut hormone response and has potential to improve dietary strategies for prevention and treatment of cardiometabolic diseases. This study was registered at clinicaltrials.gov as NCT03994276.PMID:36811474 | DOI:10.1016/j.ajcnut.2022.12.008 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 22, 2023 Category: Nutrition Authors: Balazs H Bajka Ana M Pinto Natalia Perez-Moral Shikha Saha Peter Ryden Jennifer Ahn-Jarvis Alice van der Schoot Catherine Bland Sarah E Berry Peter R Ellis Cathrina H Edwards Source Type: research
Enhanced secretion of satiety-promoting gut hormones in healthy humans after consumption of white bread enriched with cellular chickpea flour: A randomized crossover study
CONCLUSIONS: The novel use of intact chickpea cells to replace refined flours in a white bread stimulates an anorexigenic gut hormone response and has potential to improve dietary strategies for prevention and treatment of cardiometabolic diseases. This study was registered at clinicaltrials.gov as NCT03994276.PMID:36811474 | DOI:10.1016/j.ajcnut.2022.12.008 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 22, 2023 Category: Nutrition Authors: Balazs H Bajka Ana M Pinto Natalia Perez-Moral Shikha Saha Peter Ryden Jennifer Ahn-Jarvis Alice van der Schoot Catherine Bland Sarah E Berry Peter R Ellis Cathrina H Edwards Source Type: research
Genome-wide identification of the fibrillin gene family in chickpea (Cicer arietinum L.) and its response to drought stress
In this study, we identified 12 potential CaFBNs that are ranging from 25 kDa-42.92 kDa and are mostly basic. These proteins were hydrophilic in nature and generally resided in the chloroplast. The CaFBN genes were located on different chromosomes like 1, 4, 5, and 7. All FBNs shared conserved motifs and possessed a higher number of stress-responsive elements. For evolutionary analysis, a phylogenetic tree of CaFBNs with other plants' FBNs was constructed and clustered into 11 FBN subgroups. For expression analysis, 21 day old chickpea seedling was exposed to dehydration stress by withholding water. We also performed vario...
Source: International Journal of Biological Macromolecules - February 22, 2023 Category: Biochemistry Authors: Anuradha Pandey Punam Sharma Divya Mishra Sharmistha Dey Rinku Malviya Dipak Gayen Source Type: research
Global distribution, traditional and modern detection, diagnostic, and management approaches of Rhizoctonia solani associated with legume crops
Sustainable development relies heavily on a food system that is both safe and secure. Several approaches may lead to sustainability and food safety. An increase in the cultivation of legume crops is one of the approaches for enhancing agricultural viability and ensuring adequate food supply. Legumes may increase daily intake of fiber, folate, and protein as substitutes for meat and dairy. They are also crucial in various intercropping systems worldwide. However, legume production has been hampered by Rhizoctonia solani due to its destructive lifestyle. R. solani causes blights, damping off, and rotting diseases in legume c...
Source: Frontiers in Microbiology - February 6, 2023 Category: Microbiology Source Type: research
Reconnoitering the capabilities of nodule endophytic Pantoea dispersa for improved nodulation and grain yield of chickpea (Cicer arietinum L.)
This study aimed to isolate endophytic bacteria from the root nodules of chickpea (Cicer arietinum L.) and determine their potential in improving plant growth. A total of nineteen different bacterial morphotypes were isolated from root nodules of chickpea and characterized in vitro for plant growth promotion abilities. All bacterial isolates were able to produce indole acetic acid at varying levels, out of which MCA19 was screened as the most efficient indole acetic acid producer (10.25 µg mL−1). MCA8, MCA9, MCA10, MCA11, MCA16, MCA17 and MCA19 were positive for phosphate solubilization, out of which MCA9 was best pho...
Source: World Journal of Microbiology and Biotechnology - January 27, 2023 Category: Microbiology Source Type: research
Genes, Vol. 14, Pages 316: Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea
This study reports the inheritance of Ascochyta blight resistance of two wide crosses between the cultivar Gokce and wild chickpea accessions of C. reticulatum and C. echinospermum under field conditions in Southern Turkey. Following inoculation, infection damage was scored weekly for six weeks. The families were genotyped for 60 SNPs mapped to the reference genome for quantitative locus (QTL) mapping of resistance. Family lines showed broad resistance score distributions. A late responding QTL on chromosome 7 was identified in the C. reticulatum family and three early responding QTLs on chromosomes 2, 3, and 6 in the C. e...
Source: Genes - January 26, 2023 Category: Genetics & Stem Cells Authors: Abdulkarim Lakmes Abdullah Jhar Adrian C. Brennan Abdullah Kahriman Tags: Article Source Type: research
