Candida glabrata olecranon bursitis treated with bursectomy and intravenous caspofungin.
Authors: Skedros JG, Keenan KE, Trachtenberg JD Abstract Orthopedic surgeons are becoming more involved in the care of patients with septic arthritis and bursitis caused by yeast species. This case report involves a middle-aged immunocompromised female who developed a Candida glabrata septic olecranon bursitis that developed after she received a corticosteroid injection in the olecranon bursa for presumed aseptic bursitis. Candida (Torulopsis) glabrata is the second most frequently isolated Candida species from the bloodstream in the United States. Increased use of fluconazole and other azole antifungal agents as a...
Source: Journal of surgical orthopaedic advances - June 5, 2015 Category: Orthopaedics Tags: J Surg Orthop Adv Source Type: research

Trends in antifungal susceptibility of Candida species--one year observation.
Authors: Gołaś M, Netsvyetayeva I, Sikora M, Piskorska K, Sulik-Tyszka B, Swoboda-Kopeć E Abstract In the past years opportunistic fungal infections have seriously increased, mainly in immunocompromised patients. The aim of the study was to determine the prevalence of yeast-like fungi in invasive candidiasis and to estimate its susceptibility to chosen antifungal agents. One hundred and sixty strains of yeast-like fungi were cultured from various clinical material: samples from lower respiratory tract, blood, the peritoneal cavity and others. The susceptibility tests were established according to the quantitativ...
Source: Polish Journal of Microbiology - June 3, 2015 Category: Microbiology Tags: Pol J Microbiol Source Type: research

New monomeric and dimeric uridinyl derivatives as inhibitors of chitin synthase.
This study described the synthesis and in vitro evaluation of eight new derivatives of uridine as antifungal agents and inhibitors of chitin synthase. Dimeric uridinyl derivatives synthesized by us did not exhibit significant activity. One of the studied monomeric derivative, 5'-(N-succinyl)-5'-amino-5'-deoxyuridine methyl ester (compound 7) showed activities against several fungal strains (MIC range 0.06-1.00mg/mL) and inhibited chitin synthase from Saccharomyces cerevisiae (IC50=0.8mM). Moreover compound 7 exhibited synergistic interaction with caspofungin against Candida albicans (FIC index=0.28). PMID: 26051755 [P...
Source: Bioorganic Chemistry - May 22, 2015 Category: Chemistry Authors: Kral K, Bieg T, Nawrot U, Włodarczyk K, Lalik A, Hahn P, Wandzik I Tags: Bioorg Chem Source Type: research

Treatment outcomes of surgery, antifungal therapy and immunotherapy in ocular and vascular human pythiosis: a retrospective study of 18 patients
We present the results of our institutional pythiosis treatment protocol, composed of surgery, antifungal agents, iron chelator (only vascular cases) and immunotherapy. Methods We retrospectively analysed patients with proven vascular and ocular pythiosis in King Chulalongkorn Memorial Hospital from April 2003 to May 2013. Fisher's exact test and Wilcoxon's rank-sum test were used. The MICs of seven antifungal agents and combination drugs were investigated in eight clinical Pythium insidiosum strains. Results Eighteen patients were evaluated. Disease-free surgical margins were obtained in all surviving patients with vasc...
Source: Journal of Antimicrobial Chemotherapy - May 20, 2015 Category: Microbiology Authors: Permpalung, N., Worasilchai, N., Plongla, R., Upala, S., Sanguankeo, A., Paitoonpong, L., Mendoza, L., Chindamporn, A. Tags: Original research Source Type: research

Introduction of an antifungal stewardship programme targeting high-cost antifungals at a tertiary hospital in Cambridge, England
Conclusions Using a multidisciplinary team, antifungal stewardship can achieve significant improvements in patient management and it may reduce costs. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - May 20, 2015 Category: Microbiology Authors: Micallef, C., Aliyu, S. H., Santos, R., Brown, N. M., Rosembert, D., Enoch, D. A. Tags: Original research Source Type: research

Susceptibility to echinocandins of Candida spp. strains isolated in Italy assessed by European Committee for Antimicrobial Susceptibility Testing and Clinical Laboratory Standards Institute broth microdilution methods
Background: The echinocandins are recommended as first-line therapy for Candida species infections, but drug resistance, especially among Candida glabrata, is becoming more frequent. We investigated the antifungal susceptibility of anidulafungin, caspofungin, and micafungin against 584 isolates of Candida spp. (bloodstream, other sterile sites) collected from patients admitted to an Italian university hospital between 2000 and 2013. The susceptibility was evaluated using the broth microdilution method according to both the European Committee for Antimicrobial Susceptibility Testing (EUCAST EDef 7.2) and the Clinical Labora...
Source: BMC Microbiology - May 20, 2015 Category: Microbiology Authors: Maria MontagnaGrazia LoveroCaterina CorettiDomenico MartinelliOsvalda De GiglioRoberta IattaStella BalbinoAntonio RosatoGiuseppina Caggiano Source Type: research

An audit evaluating adherence to anti-fungal therapy in paediatric stem cell transplant (sct) patients
Conclusion The audit has shown that the current SOP needs to include where and what agents should be used should deviation from standard protocol become necessary such as when patients are unable to tolerate certain medication due to contra-indications or side effects. (Source: Archives of Disease in Childhood)
Source: Archives of Disease in Childhood - May 18, 2015 Category: Pediatrics Authors: Lumb, P. Tags: Posters Source Type: research

Experience of the effects of caspofungin on liver function tests in neonates
Conclusion In the babies included in this small review, we found caspofungin to be well tolerated and any initial increase in liver function tests to be transient in nature. (Source: Archives of Disease in Childhood)
Source: Archives of Disease in Childhood - May 18, 2015 Category: Pediatrics Authors: Alrifai, A., Seddon, S., Robertson, F. Tags: Posters Source Type: research

Multidrug-Resistant Candida auris Misidentified as Candida haemulonii: Characterization by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry and DNA Sequencing and Its Antifungal Susceptibility Profile Variability by Vitek 2, CLSI Broth Microdilution, and Etest Method [Mycology]
Candida auris is a multidrug-resistant yeast that causes a wide spectrum of infections, especially in intensive care settings. We investigated C. auris prevalence among 102 clinical isolates previously identified as Candida haemulonii or Candida famata by the Vitek 2 system. Internal transcribed spacer region (ITS) sequencing confirmed 88.2% of the isolates as C. auris, and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) easily separated all related species, viz., C. auris (n = 90), C. haemulonii (n = 6), C. haemulonii var. vulnera (n = 1), and Candida duobushaemulonii (n =...
Source: Journal of Clinical Microbiology - May 14, 2015 Category: Microbiology Authors: Kathuria, S., Singh, P. K., Sharma, C., Prakash, A., Masih, A., Kumar, A., Meis, J. F., Chowdhary, A. Tags: Mycology Source Type: research

Cost analysis of Voriconazole versus Caspofungin for Primary Therapy of invasive Aspergillosis among High-Risk Hematologic Cancer Patients in China
Published real-world studies suggested voriconazole (Vfend) may provide reduced length-of-stay, intravenous (IV) length-of-therapy (LOT), and costs compared to caspofungin (Cancidas) in invasive aspergillosis (IA) patients. We performed a cost analysis of voriconazole versus caspofungin as first-line antifungal treatment for IA among patients with prolonged neutropenia or undergoing bone marrow or hematopoietic stem-cell transplantation (BMT/HSCT) from a Chinese hospital perspective. (Source: Value in Health)
Source: Value in Health - May 1, 2015 Category: Global & Universal Authors: X. Gao, M. Xue, J. Stephens, Y. Chen, S. Haider, C. Charbonneau Source Type: research

Anidulafungin in Comparison to Fluconazole, Amphotericin B Deoxycholate, Amphotericin b Liposomal, Caspofungin And Micafungin For The Treatment of Invasive Candidiasis Among Non-Neutropenicpatients Above 16 Years: Bayesian Mixed Treatment Comparison (Mtc)
Invasive candidiasis is an important cause of complication and mortality of inpatient. Anidulafungin as others echinocandins are drugs that have shown clinical relevance in the treatment of invasive candidiasis. This research sought to evaluate the effectiveness and safety of anidulafungin in comparison to fluconazole, amphotericin B deoxycholate, amphotericin B liposomal, caspofungin and micafungin for the treatment of invasive candidiasis among non-neutropenicpatients above 16 years. (Source: Value in Health)
Source: Value in Health - May 1, 2015 Category: Global & Universal Authors: J.A. Diaz, C. Huerfano, C.P. Niño, J.P. Alzate, J.R. Urrego-Novoa, J.A. Moreno, V.A. Prieto Source Type: research

Impact of short‐term exposure of antifungal agents on hemolysin activity of oral Candida dubliniensis isolates from Kuwait and Sri Lanka
ConclusionBrief exposure to sublethal concentrations of drugs with antifungal properties appears to reduce the pathogenic potential of C. dubliniensis isolates by suppressing hemolysin production. (Source: Journal of Investigative and Clinical Dentistry)
Source: Journal of Investigative and Clinical Dentistry - May 1, 2015 Category: Dentistry Authors: Arjuna N. B. Ellepola, J. A. M. Sumedha Jayathilake, Prem N. Sharma, Zia U. Khan Tags: Original Article Source Type: research

Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo
This article is protected by copyright. All rights reserved. (Source: Photochemistry and Photobiology)
Source: Photochemistry and Photobiology - May 1, 2015 Category: Science Authors: Lucia Černáková, Jaroslava Chupáčová, Katarína Židlíková, Helena Bujdáková Tags: Research Article Source Type: research

Outcomes associated with routine systemic antifungal therapy in critically ill patients with Candida colonization
Conclusions Preemptive strategy of antifungal drug prescriptions in highly colonized ICU patients induced an increase in C. glabrata colonization without significant shift of colonization to other Candida spp. in surgical ICU patients. However, the potential detrimental impact of fluconazole on Candida ecology in ICU and/or on Candida susceptibility to antifungal drugs should be considered, and deserves further studies. (Source: Intensive Care Medicine)
Source: Intensive Care Medicine - April 18, 2015 Category: Intensive Care Source Type: research

Economic Comparison of an Empirical Versus Diagnostic-Driven Strategy for Treating Invasive Fungal Disease in Immunocompromised Patients.
This study suggests that a DD strategy is likely to be cost-saving versus empirical treatment for immunocompromised patients with persistent or recurrent neutropenic fevers. PMID: 25891805 [PubMed - as supplied by publisher] (Source: Clinical Therapeutics)
Source: Clinical Therapeutics - April 16, 2015 Category: Drugs & Pharmacology Authors: Barnes R, Earnshaw S, Herbrecht R, Morrissey O, Slavin M, Bow E, McDade C, Charbonneau C, Weinstein D, Kantecki M, Schlamm H, Maertens J Tags: Clin Ther Source Type: research

Protein bound drugs are prone to sequestration in the extracorporeal membrane oxygenation circuit: results from an ex vivo study
IntroductionVital drugs may be degraded or sequestered in extracorporeal membrane oxygenation (ECMO) circuits with lipophilic drugs considered to be particularly vulnerable. However, the circuit effects on protein bound drugs have not been fully elucidated. This experiment aimed to investigate the influence of plasma protein binding on drug disposition in ex vivo ECMO circuits. Methods: Four identical ECMO circuits comprising centrifugal pumps and polymethyl pentene oxygenators and were utilised. The circuits were primed with crystalloid, albumin and fresh human whole blood and maintained at a physiological pH and temperat...
Source: Critical Care - April 14, 2015 Category: Intensive Care Authors: Kiran ShekarJason RobertsCharles McdonaldSussan GhassabianChris AnsteySteven WallisDaniel MullanyYoke FungJohn Fraser Source Type: research

Antifungal activity of compounds targeting the Hsp90-calcineurin pathway against various mould species
Conclusions Targeting the Hsp90-calcineurin axis at different levels resulted in distinct patterns of susceptibility among different fungal species. Lysine deacetylase inhibition may represent a promising novel antifungal strategy against emerging resistant moulds. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - April 13, 2015 Category: Microbiology Authors: Lamoth, F., Alexander, B. D., Juvvadi, P. R., Steinbach, W. J. Tags: Original research Source Type: research

High-Dose Caspofungin as a Component of Combination Antifungal Therapy in 91 Patients With Neoplastic Diseases and Hematopoietic Stem Cell Transplantation: A Critical Review of Short-Term and Long-Term Adverse Events
The antifungal activity of echinocandins is concentration dependent. Previously, we demonstrated that high-dose caspofungin (HD-CSP; 100 mg daily) was well tolerated in 34 immunosuppressed patients with cancer and may have favorably influenced outcomes. We retrospectively assessed all 91 patients in whom HD-CSP was given for the treatment of invasive fungal disease (IFD). The median number of doses was 18.5 ± 21.5, and in 8 (9%) patients more than 40 doses were given. Most (62%) of the patients had leukemia. A total of 45 (49%) patients had undergone stem cell transplantation; 80% received allogeneic grafts and 47% ...
Source: Journal of Pharmacy Practice - April 12, 2015 Category: Drugs & Pharmacology Authors: Safdar, A., Rodriguez, G., Zuniga, J., Al Akhrass, F., Pande, A. Tags: Research Articles Source Type: research

The Eagle-Like Effect of the Echinocandins: Is It Relevant for Clinical Decisions?
Abstract The Eagle effect is a paradoxical growth phenomenon long known to exist in vitro with specific antimicrobial agents, particularly the penicillins. More recent reports have indicated that this effect also exists when Candida or Aspergillus species are exposed to the echinocandin antifungals. While the majority of data for this effect exists for caspofungin, there are also reports that micafungin and anidulafungin may have a paradoxical growth effect in certain media or with particular pathogens. In efforts to reproduce the paradoxical growth curve in vivo, results with Candida have been varied. In vivo stu...
Source: Current Fungal Infection Reports - April 9, 2015 Category: Infectious Diseases Source Type: research

Invasive Saccharomyces cerevisiae in a liver transplant patient: case report and review of infection in transplant recipients
This article is protected by copyright. All rights reserved. (Source: Transplant Infectious Disease)
Source: Transplant Infectious Disease - April 1, 2015 Category: Transplant Surgery Authors: K.Y. Popiel, P. Wong, M.J. Lee, M. Langelier, D.C. Sheppard, D.C. Vinh Tags: Case Report Source Type: research

Novel Antifungal Drugs Against Fungal Pathogens: Do They Provide Promising Results for Treatment?
Abstract The febrile neutropenia episodes of hematological patients and their outcomes were evaluated with respect to fungal pathogens and antifungal therapy in this retrospective study. All patients, who were older than 14 years of age and developed at least one neutropenic episode after chemotherapy due to hematological cancer from November 2010 to November 2012, were included into the study. We retrospectively collected demographic, treatment, and survival data of 126 patients with neutropenia and their 282 febrile episodes. The mean Multinational Association for Supportive Care in Cancer score was 17.18&...
Source: Indian Journal of Hematology and Blood Transfusion - March 27, 2015 Category: Hematology Source Type: research

Our 2015 approach to invasive pulmonary aspergillosis
Summary At the University Hospital of Cologne, in general two patient groups at high risk for invasive aspergillosis receive posaconazole prophylaxis: Acute myelogenous leukaemia patients during remission induction chemotherapy and allogeneic haematopoietic stem cell transplant recipients. Other patients at risk undergo serum galactomannan testing three times weekly. At 72–96 h of persisting fever despite broad‐spectrum antibiotics, or at onset of lower respiratory tract symptoms a thoracic computed tomography (CT) scan is performed. Without lung infiltrates on CT, IPA is ruled out. In lung infiltrates not sug...
Source: Mycoses - March 24, 2015 Category: Research Authors: B. Liss, J. J. Vehreschild, C. Bangard, D. Maintz, K. Frank, S. Grönke, G. Michels, A. Hamprecht, H. Wisplinghoff, B. Markiefka, K. Hekmat, M. J. G. T. Vehreschild, O. A. Cornely Tags: Review Article Source Type: research

Antifungal susceptibility and phenotypic characterization of oral isolates of a black fungus from a nasopharyngeal carcinoma patient under radiotherapy
Conclusion: We, for the first time, reported the discovery of a black fungus–E. dermatitidis isolates derived from a patient with nasopharyngeal carcinoma treated with radiotherapy. These isolates were shown to be resistant to caspofungin, a major antifungal agent for systemic candidiasis. As little is known about the black fungus in the clinical setting, it is important that clinicians must keep abreast of the new discovery in this field. (Source: BMC Oral Health)
Source: BMC Oral Health - March 20, 2015 Category: Dentistry Authors: Chaminda SeneviratnePhoenix FongSarah WongVictor Lee Source Type: research

How should we manage a patient with invasive mucoromycosis who Develops life-threatening reaction to amphotericin B? report of two cases and literature review
This report presents two cases of invasive rhino-orbital mucormycosis who had life-threatening reactions to amphotericin B. Both cases were treated with a combination of posaconazole-caspofungin favorably with no evidence of recurrence upon long-term follow-up. To our knowledge, this is the first report of successful treatment of invasive mucormycosis with azole-echinocandin combination. It may suggest that caspofungin exerts additional or even synergistic antimucoral effects to posaconazole. (Source: Medical Mycology Case Reports)
Source: Medical Mycology Case Reports - March 12, 2015 Category: Biology Source Type: research

Randomized Trial of Micafungin for the Prevention of Invasive Fungal Infection in High-Risk Liver Transplant Recipients
Conclusions. Micafungin was noninferior to standard care as antifungal prophylaxis in liver transplant patients at high risk for IFI. Adverse event profiles and liver function at EOP were similar, although kidney function was better with micafungin. Clinical Trials Registration. NCT01058174. (Source: Clinical Infectious Diseases)
Source: Clinical Infectious Diseases - March 12, 2015 Category: Infectious Diseases Authors: Saliba, F., Pascher, A., Cointault, O., Laterre, P.-F., Cervera, C., De Waele, J. J., Cillo, U., Langer, R. M., Lugano, M., Goran-Ericzon, B., Phillips, S., Tweddle, L., Karas, A., Brown, M., Fischer, L., for the TENPIN (Liver Transplant European Study In Tags: ARTICLES AND COMMENTARIES Source Type: research

In vitro antifungal susceptibility of Trichophyton violaceum isolated from tinea capitis patients
Conclusions Posaconazole, terbinafine and voriconazole were shown to be the most potent antifungal agents against T. violaceum isolates obtained from tinea capitis patients worldwide. These results might help clinicians in developing appropriate therapies that have a high probability of successfully treating tinea capitis due to T. violaceum. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - March 11, 2015 Category: Microbiology Authors: Deng, S., de Hoog, G. S., Verweij, P. E., Zoll, J., Ilkit, M., Morsali, F., Abliz, P., Wang, X., Zhan, P., Yang, L., Hasimu, H., Liao, W., Pan, W., Seyedmousavi, S. Tags: Original research Source Type: research

In vitro susceptibility patterns of clinically important Trichophyton and Epidermophyton species against nine antifungal drugs
Summary Despite the common, worldwide, occurrence of dermatophytes, little information is available regarding susceptibility profiles against currently available and novel antifungal agents. A collection of sixty‐eight clinical Trichophyton species and Epidermophyton floccosum were previously identified and verified to the species level by sequencing the internal transcribed spacer (ITS) regions of rDNA. MICs of amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, isavuconazole, terbinafine and MECs of caspofungin and anidulafungin were performed based on CLSI M38‐A2. The resulting MIC90s of all strai...
Source: Mycoses - March 10, 2015 Category: Research Authors: Hamid Badali, Rasoul Mohammadi, Olga Mashedi, G. Sybren Hoog, Jacques F. Meis Tags: Original Article Source Type: research

Fungal β-1,3-glucan increases ofloxacin-tolerance of Escherichia coli in a polymicrobial E. coli - Candida albicans biofilm.
In this study we show that Escherichia coli does not affect Candida albicans tolerance to amphotericin or caspofungin in an E. coli/C. albicans biofilm. In contrast, ofloxacin-tolerance of E. coli is significantly increased in a polymicrobial E. coli/C. albicans biofilm as compared to an axenic E. coli biofilm. The increased ofloxacin-tolerance of E. coli is mainly biofilm-specific as ofloxacin-tolerance of E. coli is less pronounced in polymicrobial E. coli/C. albicans planktonic cultures. Moreover, we found that ofloxacin-tolerance of E. coli decreased significantly when E. coli/C. albicans biofilms were treated with mat...
Source: Antimicrobial Agents and Chemotherapy - March 9, 2015 Category: Microbiology Authors: De Brucker K, Tan Y, Vints K, De Cremer K, Braem A, Verstraeten N, Michiels J, Vleugels J, Cammue BP, Thevissen K Tags: Antimicrob Agents Chemother Source Type: research

Efficacy of the Investigational Echinocandin ASP9726 in a Guinea Pig Model of Invasive Pulmonary Aspergillosis.
Abstract ASP9726 is an investigational echinocandin with in vitro activity against Aspergillus species. We evaluated the pharmacokinetics and efficacy of this agent in a established guinea pig model of invasive pulmonary aspergillosis. ASP9726 plasma concentrations were measured in guinea pigs administered either a single dose or multiple doses of this agent at 2.5, 5, & 10 mg/kg/day by subcutaneous injection. Immunosuppressed guinea pigs were inoculated with A. fumigatus AF293, and treatment with ASP9726 (2.5, 5, & 10 mg/kg/day), voriconazole (10 mg/kg by oral gavage twice daily), or caspofungin (3 mg/kg/...
Source: Antimicrobial Agents and Chemotherapy - March 9, 2015 Category: Microbiology Authors: Wiederhold NP, Najvar LK, Matsumoto S, Bocanegra RA, Herrera ML, Wickes BL, Kirkpatrick WR, Patterson TF Tags: Antimicrob Agents Chemother Source Type: research

Pseudozyma aphidis Fungemia after abdominal surgery: First adult case
We report a case of P. aphidis invasive infection in an adult with a metastatic ampulloma who had gone through digestive surgery. (Source: Medical Mycology Case Reports)
Source: Medical Mycology Case Reports - March 7, 2015 Category: Biology Source Type: research

Combined medico‐surgical strategy for invasive sino‐orbito‐cerebral breakthrough fungal infection with Hormographiella aspergillata in an acute leukaemia patient
We report a neutropenic patient who developed a severe sino‐orbito‐cerebral H. aspergillata infection while receiving empirical caspofungin, successfully controlled by an aggressive strategy associating surgical debridement and combined high‐dose regimen of antifungal drugs. (Source: Mycoses)
Source: Mycoses - March 6, 2015 Category: Research Authors: M. Heiblig, V. Bozzoli, J. Saison, X. Thomas, D. De Croze, A. Traverse‐Glehen, A. Cosmidis, C. Chidiac, T. Ferry, A. Alanio, A. L. Bienvenu, D. Dupont, S. Ducastelle‐Lepretre, M. Michallet, F. Ader, Tags: Case Report Source Type: research

Primary Aspergillus Sellar Abscess Simulating Pituitary Tumor in Immunocompetent Patient
Abstract: A 55-year-old woman presented with headache, dizziness, and decreased visual acuity. Magnetic resonance imaging revealed a sellar mass with sphenoid sinus extension. The result of hormone showed an obviously high prolactin (815 ng/mL). The mass was resected and diagnosed with aspergillosis pathologically. Postoperatively, the level of prolactin dramatically decreased, and the patient received medical treatment with voriconazole and caspofungin. During a 6-month follow-up, the patient’s headache and dizziness disappeared, and visual acuity improved. Therefore, aspergillus sellar abscess could result in hyper...
Source: Journal of Craniofacial Surgery - March 1, 2015 Category: Surgery Tags: Brief Clinical Studies Source Type: research

New echinocandin susceptibility patterns for nosocomial Candida albicans in Bogotá, Colombia, in ten tertiary care centres: an observational study
Conclusions: Significant differences were found regarding susceptibility results when comparing CLSI M27-A3 to CLSI M27-S4 when shifting clinical break-point values. However, one nosocomial strain was consistent in having reduced susceptibility when using both guidelines without having been directly exposed to echinocandins beforehand and no mutations were found in the FKS1 gene for hot spot 1 and/or hot spot 2 regions, thereby highlighting selective pressure regarding widespread antifungal use in tertiary healthcare centres. Nutritional conditions and low family income were seen to have a negative effect on survival rates...
Source: BMC Infectious Diseases - February 28, 2015 Category: Infectious Diseases Authors: Giovanni Rodríguez-LeguizamónAlessandro FioriKatrien LagrouMaría GaonaMilciades IbáñezManuel PatarroyoPatrick Van DijckArley Gómez-López Source Type: research

Antifungal Susceptibilities of Bloodstream Isolates of Candida Species from Nine Hospitals in Korea: Application of New Antifungal Breakpoints and Relationship to Antifungal Usage
by Eun Jeong Won, Jong Hee Shin, Min Ji Choi, Wee Gyo Lee, Yeon-Joon Park, Young Uh, Shine-Young Kim, Mi-Kyung Lee, Soo Hyun Kim, Myung Geun Shin, Soon Pal Suh, Dong Wook Ryang We applied the new clinical breakpoints (CBPs) of the Clinical and Laboratory Standards Institute (CLSI) to a multicenter study to determine the antifungal susceptibility of bloodstream infection (BSI) isolates of Candida species in Korea, and determined the relationship between the frequency of antifungal-resistant Candida BSI isolates and antifungal use at hospitals. Four hundred and fifty BSI isolates of Candida species were collected over a 1-y...
Source: PLoS One - February 23, 2015 Category: Biomedical Science Authors: Eun Jeong Won et al. Source Type: research

Candida fermentati as a Cause of Persistent Fungemia in a Preterm Neonate Successfully Treated by Combination Therapy with Amphotericin B and Caspofungin [Case Reports]
A case of persistent candidemia in a preterm neonate caused by Candida fermentati, identified by sequencing of the internally transcribed spacer region of ribosomal DNA (rDNA), is described. The neonate was treated for 30 days by combination therapy with amphotericin B (AmBisome) and caspofungin with a successful outcome, and no drug-related side effects were observed. (Source: Journal of Clinical Microbiology)
Source: Journal of Clinical Microbiology - February 19, 2015 Category: Microbiology Authors: Al-Sweih, N., Ahmad, S., Joseph, L., Khan, S., Vayalil, S., Chandy, R., Khan, Z. Tags: Case Reports Source Type: research

Activation and Alliance of Regulatory Pathways in C. albicans during Mammalian Infection
by Wenjie Xu, Norma V. Solis, Rachel L. Ehrlich, Carol A. Woolford, Scott G. Filler, Aaron P. Mitchell Gene expression dynamics have provided foundational insight into almost all biological processes. Here, we analyze expression of environmentally responsive genes and transcription factor genes to infer signals and pathways that drive pathogen gene regulation during invasive Candida albicans infection of a mammalian host. Environmentally responsive gene expression shows that there are early and late phases of infection. The early phase includes induction of zinc and iron limitation genes, genes that respond to transcripti...
Source: PLoS Biology: Archived Table of Contents - February 18, 2015 Category: Biology Authors: Wenjie Xu et al. Source Type: research

Voriconazole‐induced periostitis after allogeneic stem cell transplantation
This article is protected by copyright. All rights reserved. (Source: American Journal of Hematology)
Source: American Journal of Hematology - February 14, 2015 Category: Hematology Authors: John H. Baird, Belinda K. Birnbaum, David L. Porter, Noelle V. Frey Tags: Diagnostic Imaging in Hematology Source Type: research

Pneumocystis Encodes a Functional Endo-{beta}-1,3-glucanase That is Expressed Exclusively in Cysts
β-1,3-glucan is a major cell wall component of Pneumocystis cysts. We have characterized endo-β-1,3-glucanase (Eng) from 3 species of Pneumocystis. The gene eng is a single-copy gene that encodes a protein containing 786 amino acids in P. carinii and P. murina, and 788 amino acids in P. jirovecii, including a signal peptide for the former 2 but not the latter. Recombinant Eng expressed in Escherichia coli was able to solubilize the major surface glycoprotein of Pneumocystis, thus potentially facilitating switching of the expressed major surface glycoprotein (Msg) variant. Confocal immunofluorescence analysis of P...
Source: The Journal of Infectious Diseases - February 6, 2015 Category: Infectious Diseases Authors: Kutty, G., Davis, A. S., Ma, L., Taubenberger, J. K., Kovacs, J. A. Tags: FUNGI Source Type: research

Antifungal susceptibilities of Candida glabrata species complex, Candida krusei, Candida parapsilosis species complex and Candida tropicalis causing invasive candidiasis in China: 3 year national surveillance
Conclusions In summary, reduced azole susceptibility was seen among C. tropicalis. Resistance to echinocandins was uncommon. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - February 6, 2015 Category: Microbiology Authors: Xiao, M., Fan, X., Chen, S. C.- A., Wang, H., Sun, Z.-Y., Liao, K., Chen, S.-L., Yan, Y., Kang, M., Hu, Z.-D., Chu, Y.-Z., Hu, T.-S., Ni, Y.-X., Zou, G.-L., Kong, F., Xu, Y.-C. Tags: Original research Source Type: research

Pharmacokinetic variability and exposures of fluconazole, anidulafungin and caspofungin in intensive care unit patients: Data from multinational Defining Antibiotic Levels in Intensive care unit (DALI) patients Study
Conclusions: Considerable inter-individual variability was observed for fluconazole, anidulafungin and caspofungin. A large proportion of the patients (33%) receiving fluconazole did not attain the PK/PD target which might be related to inadequate dosing. For anidulafungin and caspofungin, dose optimisation also appears necessary to minimize variability. (Source: BioMed Central)
Source: BioMed Central - February 4, 2015 Category: Journals (General) Authors: Mahipal G SinnollareddyJason A RobertsJeffrey LipmanMurat AkovaMatteo BassettiJan J De WaeleKirsi-Maija KaukonenDespoina KoulentiClaude MartinPhilippe MontraversJordi RelloAndrew RhodesTherese StarrSteven C WallisGeorge Dimopouloson behalf of the DALI Stu Source Type: research

Caspofungin irrigation through percutaneous calicostomy catheter combined with oral flucytosine to treat fluconazole-resistant symptomatic candiduria
We report a case of recurrent Candida parapsilosis candiduria in a kidney transplant recipient suffering from chronic ureteral obstruction requiring permanent ureteral catheterization (double-J stent). Attempts to remove the stent led to pyelonephritis episodes during which only Candida was isolated from the urine. Following several courses of azole-based therapy, the causative agent became resistant to fluconazole. Clinical and mycological cure were obtained combining irrigations of caspofungin through a percutaneous calicostomy catheter and oral flucytosine. This strategy may represent an interesting therapeutic alternat...
Source: Journal of Medical Mycology - January 31, 2015 Category: Infectious Diseases Source Type: research

Evaluation of antifungal combinations of nystatin-intralipid against Aspergillus terreus using checkerboard and disk diffusion methods
Conclusions Among four tested combinations, only combination of nytatin-intralipid with caspofungin, a representative of the echinocandin class of antifungals, resulted in synergistic interaction. Antagonism obtained by combining nystatin-intralipid with terbinafine can be explained by existence of hydrophobic interaction between these two compounds interfering with their antifungal action. The fact that nystatin-intralipid and nystatin interact differently with other antifungals, may indicate differences in their mechanisms of activity. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - January 30, 2015 Category: Infectious Diseases Source Type: research

Identification and Susceptibility of Aspergillus Section Nigri in China: Prevalence of Species and Paradoxical Growth in Response to Echinocandins [Mycology]
Molecular identification and in vitro antifungal susceptibility tests of 43 Aspergillus section Nigri isolates from China were performed. Aspergillus niger and Aspergillus tubingensis were present in almost equal numbers. All of the isolates had low MIC/MECs (minimum effective concentrations) for the 7 common antifungals, and a paradoxical effect was observed for the first time in response to caspofungin and micafungin. (Source: Journal of Clinical Microbiology)
Source: Journal of Clinical Microbiology - January 23, 2015 Category: Microbiology Authors: Li, Y., Wan, Z., Liu, W., Li, R. Tags: Mycology Source Type: research

Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China.
Abstract  There is limited information regarding molecular epidemiology and antifungal susceptibilities of Candida isolates using Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using Neo-Sensitabs tablet diffusion method. Candida species were isolated f...
Source: Journal of Medical Microbiology - January 16, 2015 Category: Microbiology Authors: Shi XY, Yang YP, Zhang Y, Li W, Wang JD, Huang WM, Fan YM Tags: J Med Microbiol Source Type: research

Empirical caspofungin therapy in clinical practice for suspected invasive fungal disease in adults with acute lymphoblastic leukaemia
Summary Patients with acute lymphoblastic leukaemia (ALL) after cytotoxic chemotherapy or haematopoietic stem cell transplantation (HSCT) are at risk for life‐threatening invasive fungal disease (IFD). The aim was to evaluate the characteristics, antifungal therapy and outcome of adult patients with ALL after chemotherapy or HSCT receiving caspofungin empirically in a clinical setting. Retrospective chart reviews were conducted at nine large tertiary care centres in Germany. Adult patients with ALL treated empirically with caspofungin according to the product label between 2006 and 2012 were eligible. Data were extracted...
Source: Mycoses - January 15, 2015 Category: Research Authors: Michael G. Kiehl, Gerlinde Egerer, Monika Engelhardt, Barbara Gross Tags: Original Article Source Type: research

The Strength of Synergistic Interaction between Posaconazole and Caspofungin depends on the Underlying Azole-Resistance Mechanism of Aspergillus fumigatus.
Abstract The majority of azole resistance mechanisms in Aspergillus fumigatus account to mutations in cyp51A gene. As azoles are less effective against infections caused by multi-azole-resistant A. fumigatus isolates, new therapeutic options are warranted for treating these infections. We therefore investigated the in vitro combination of posaconazole (POSA) and caspofungin (CAS) against 20 wild-type and resistant A. fumigatus isolates with 10 different resistance mechanisms. Fungal growth was assessed with the XTT method. Pharmacodynamic interactions were assessed with the fractional inhibitory concentration inde...
Source: Antimicrobial Agents and Chemotherapy - January 12, 2015 Category: Microbiology Authors: Mavridou E, Meletiadis J, Rijs A, Mouton JW, Verweij PE Tags: Antimicrob Agents Chemother Source Type: research

Secondary Antifungal Prophylaxis in Pediatric Hematopoietic Stem Cell Transplants
In conclusion, our study shows that amphotericin B and caspofungin was successful as secondary antifungal prophylaxis agents with no relapse of IFI. In addition, after engraftment, secondary prophylaxis was continued with voriconazole orally in 4 patients that yielded good results. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - January 1, 2015 Category: Hematology Tags: Online Articles: Original Articles Source Type: research

Trichosporon asahii infection presenting as chronic meningo‐ventriculitis and intra ventricular fungal ball: a case report and literature review
We report a rare case of chronic meningo‐ventriculitis and intraventricular fungal ball due to Trichosporon asahii in an 18‐year‐old immunocompetent male from Burundi, east Africa. Neuroendoscopy showed multiple nodules and a fungal ball within the ventricle, which on culture grew T. asahii. He was initially empirically treated with liposomal amphotericin B. However, the antifungal susceptibility testing of T. asahii isolate revealed high minimum inhibitory concentration for amphotericin B (2 μg ml−1), flucytosine (16 μg ml−1) and caspofungin (2 μg ml−1) but ex...
Source: Mycoses - January 1, 2015 Category: Research Authors: Anil Kumar, Suhas Udayakumaran, Rachana Babu, Bindhu M. Rajamma, Anupam Prakash, Dilip Panikar, Shamsul Karim, Anuradha Chowdhary Tags: Case Report Source Type: research

Cardiac effects of echinocandins after central venous administration in adult rats.
Abstract Echinocandins have become the agents of choice for early and specific antifungal treatment in critically ill patients. In-vitro studies and clinical case reports revealed a possible impact of echinocandin treatment on cardiac function. The aim of our study was to evaluate echinocandin induced cardiac failure : Using an in-vivo rat model we assessed hemodynamic parameters and time to hemodynamic failure after central-venous application (Vena jugularis interna) of anidulafungin (low dose group 2.5 mg/kg bodyweight (BW), high dose group 25 mg/kg BW), caspofungin (low dose group 0.875 mg/kg BW, high dose grou...
Source: Antimicrobial Agents and Chemotherapy - December 29, 2014 Category: Microbiology Authors: Koch C, Uhle F, Wolff M, Arens C, Schulte A, Li L, Niemann B, Henrich M, Rohrbach S, Weigand MA, Lichtenstern C Tags: Antimicrob Agents Chemother Source Type: research

The impact of clinical pharmacist and ID intervention in rationalization of antimicrobial use
Publication date: December 2014 Source:Saudi Pharmaceutical Journal, Volume 22, Issue 6 Author(s): Niaz Al-Somai , Mohammed Al-Muhur , Osama Quteimat , Nashaat Hamzah What is known and objective There is little research on the impact of implementing and monitoring antimicrobial policy in Saudi hospitals. The purpose of this study is to measure the impact of the clinical pharmacist (CP) and infectious disease consultant (ID) interventions on the use of three antimicrobials (caspofungin, imipenem, meropenem) in hospitalized patients in the King Abdullah Medical City hospital. Methods The study was carried out in the King ...
Source: Saudi Pharmaceutical Journal - December 27, 2014 Category: Drugs & Pharmacology Source Type: research