Efficacy of initial caspofungin plus trimethoprim/sulfamethoxazole for severe PCP in patients without human immunodeficiency virus infection
The number of pneumocystis pneumonia (PCP) cases is increasing in immunocompromised patients without human immunodeficiency virus infection (HIV), causing serious morbidity with high mortality. Trimethoprim/su... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - June 16, 2023 Category: Infectious Diseases Authors: Hui Qi, Danjiang Dong, Ning Liu, Ying Xu, Mengzhi Qi and Qin Gu Tags: Research Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to ...
Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research
Rapid automated antifungal susceptibility testing system for yeasts based on growth characteristics
In this study, we introduce a novel automated antifungal susceptibility testing system, Droplet 48, which detects the fluorescence of microdilution wells in real time and fits growth characteristics using fluorescence intensity over time. We concluded that all reportable ranges of Droplet 48 were appropriate for clinical fungal isolates in China. Reproducibility within ±2 two-fold dilutions was 100%. Considering the Sensititre YeastOne Colorimetric Broth method as a comparator method, eight antifungal agents (fluconazole, itraconazole, voriconazole, caspofungin, micafungin, anidulafungin, amphotericin B, and 5-flucytosine...
Source: Frontiers in cellular and infection microbiology - May 2, 2023 Category: Microbiology Source Type: research
Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals
Conclusions: Resistance to triazole agents should be considered inC. tropicalis infections in the studied hospitals, and surveillance measures taken to avoidCandida diffusion. (Source: The Journal of Infection in Developing Countries)
Source: The Journal of Infection in Developing Countries - April 29, 2023 Category: Infectious Diseases Authors: Thi Minh Chau Ngo, Antonella Santona, Maura Fiamma, Phuong Anh Ton Nu, Thi Bich Thao Do, Piero Cappuccinelli, Bianca Paglietti Source Type: research
The Effects of Holothurin and Caspofungin on the Vaginal Cell Inflammation Parameters of the Rattus norvegicus Strain Post Induction of Candida albicans
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - April 27, 2023 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
LC –MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients
In this study, we developed a robust, sensitive, and simple liquid chromatography-tandem mass spectrometry (LC–MS/MS) platform for the quantification of 14 antibacterial and antifungal agents (beta-lactams piperacillin, cefoperazone, and meropenem; beta-lactamase inhibitors tazobactam and sulbactam; antifungal agents fluconazole, caspofungin, posaconazole, and voriconazole; and daptomycin, vancomycin, teicoplanin, linezolid, and tigecycline) that can be used for patients with severe infection. This assay requires only 100 µL of serum with rapid protein precipitation. Chromatographic analysis was performed using a Water...
Source: Frontiers in Pharmacology - April 18, 2023 Category: Drugs & Pharmacology Source Type: research
Aneuploidy enables cross-tolerance to unrelated antifungal drugs in Candida parapsilosis
Candida parapsilosis is an emerging major human fungal pathogen. Echinocandins are first-line antifungal drugs for the treatment of invasive Candida infections. In clinical isolates, tolerance to echinocandins in Candida species is mostly due to point mutations of FKS genes, which encode the target protein of echinocandins. However, here, we found chromosome 5 trisomy was the major mechanism of adaptation to the echinocandin drug caspofungin, and FKS mutations were rare events. Chromosome 5 trisomy conferred tolerance to echinocandin drugs caspofungin and micafungin and cross-tolerance to 5-flucytosine, another class of an...
Source: Frontiers in Microbiology - April 11, 2023 Category: Microbiology Source Type: research
In vivo evolution to echinocandin resistance and increasing clonal heterogeneity in < em > Candida auris < /em > during a difficult-to-control hospital outbreak, Italy, 2019 to 2022
Euro Surveill. 2023 Apr;28(14). doi: 10.2807/1560-7917.ES.2023.28.14.2300161.ABSTRACTA difficult-to-control outbreak of Candida auris is ongoing in a large tertiary care hospital in Liguria, Italy, where it first emerged in 2019. In a retrospective analysis, 503 cases of C. auris carriage or infection were observed between July 2019 and December 2022. Genomic surveillance identified putative cases that no longer occurred as part of one defined outbreak and the emergence of echinocandin (pandrug) resistance following independent selection of FKS1S639F and FKS1F635Y mutants upon prolonged exposure to caspofungin and/or anidu...
Source: Euro Surveill - April 6, 2023 Category: Infectious Diseases Authors: Giulia Codda Edward Willison Laura Magnasco Paola Morici Daniele Roberto Giacobbe Antonella Mencacci Daniele Marini Malgorzata Mikulska Matteo Bassetti Anna Marchese Vincenzo Di Pilato Source Type: research
In vivo evolution to echinocandin resistance and increasing clonal heterogeneity in < em > Candida auris < /em > during a difficult-to-control hospital outbreak, Italy, 2019 to 2022
Euro Surveill. 2023 Apr;28(14). doi: 10.2807/1560-7917.ES.2023.28.14.2300161.ABSTRACTA difficult-to-control outbreak of Candida auris is ongoing in a large tertiary care hospital in Liguria, Italy, where it first emerged in 2019. In a retrospective analysis, 503 cases of C. auris carriage or infection were observed between July 2019 and December 2022. Genomic surveillance identified putative cases that no longer occurred as part of one defined outbreak and the emergence of echinocandin (pandrug) resistance following independent selection of FKS1S639F and FKS1F635Y mutants upon prolonged exposure to caspofungin and/or anidu...
Source: Euro Surveill - April 6, 2023 Category: Infectious Diseases Authors: Giulia Codda Edward Willison Laura Magnasco Paola Morici Daniele Roberto Giacobbe Antonella Mencacci Daniele Marini Malgorzata Mikulska Matteo Bassetti Anna Marchese Vincenzo Di Pilato Source Type: research
