GSE100091 Unexpected bactericidal activity of caspofungin against Enterococcus faecium
Contributors : Christophe Isnard ; Sara Hernandez ; Fanny Joalland ; Didier Goux ; Fran çois Guérin ; François Gravey ; Michel Auzou ; David Enot ; Pierrick Meignen ; Felipe Cava ; Vincent CattoirSeries Type : Expression profiling by high throughput sequencingOrganism : Enterococcus faeciumEnterococcus faecium has become a major opportunistic pathogen with the emergence of multidrug-resistant clones that are well-adapted to the hospital environment. As part of the vast diversity of gut microbiota, they are faced with different environmental stress, including antimicrobial pressure. By contrast, little ...
Source: GEO: Gene Expression Omnibus - June 16, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Enterococcus faecium Source Type: research

Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells
Publication date: Available online 25 May 2017 Source:Microbes and Infection Author(s): Nansalmaa Amarsaikhan, Evan M. O’Dea, Angar Tsoggerel, Steven P. Templeton The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest. In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment. In add...
Source: Microbes and Infection - May 27, 2017 Category: Infectious Diseases Source Type: research

Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins [Mycology]
Sensititre YeastOne (YO) panels were assessed for in vitro susceptibility testing of echinocandins against 39 isolates of Aspergillus fumigatus, A. flavus, and A. terreus, including two echinocandin-resistant A. fumigatus strains, using different inocula (103, 104, and 105 CFU/ml), incubation times (16 to 48 h), and endpoints (first blue or purple well) and compared to CLSI M38-A2. The best agreement was found with an inoculum of 104 CFU/ml, incubation times of 20 h for A. flavus and of 30 h for A. fumigatus and A. terreus, and reading the first purple well. The reproducibility within ±1 2-fold dilutions was 100% fo...
Source: Journal of Clinical Microbiology - May 23, 2017 Category: Microbiology Authors: Siopi, M., Pournaras, S., Meletiadis, J. Tags: Mycology Source Type: research

In Vitro Antifungal Susceptibility of Yeast and Mold Phases of Isolates of Dimorphic Fungal Pathogen Emergomyces africanus (Formerly Emmonsia sp.) from HIV-Infected South African Patients [Mycology]
Disseminated emmonsiosis is an important AIDS-related mycosis in South Africa that is caused by Emergomyces africanus, a newly described and renamed dimorphic fungal pathogen. In vitro antifungal susceptibility data can guide management. Identification of invasive clinical isolates was confirmed phenotypically and by sequencing of the internal transcribed spacer region. Yeast and mold phase MICs of fluconazole, voriconazole, itraconazole, posaconazole, caspofungin, anidulafungin, micafungin, and flucytosine were determined with custom-made frozen broth microdilution (BMD) panels in accordance with Clinical and Laboratory S...
Source: Journal of Clinical Microbiology - May 23, 2017 Category: Microbiology Authors: Maphanga, T. G., Britz, E., Zulu, T. G., Mpembe, R. S., Naicker, S. D., Schwartz, I. S., Govender, N. P. Tags: Mycology Source Type: research

Management of Aspergillus pleural empyema with combined systemic and intrapleural antifungal therapy in a pediatric patient: Case report
We present the case of an 8-year-old immunocompromised boy with invasive pulmonary aspergillosis, complicated with pleural empyema and disseminated to the bone and central nervous system (CNS). The patient was treated with combined systemic antifungal agents (voriconazole and caspofungin) and intrapleural instillation of liposomal amphotericin B, in addition to surgical intervention. The treatment was well tolerated and resulted in remarkable clinical and radiological pulmonary improvement. (Source: Journal of Infection and Public Health)
Source: Journal of Infection and Public Health - May 22, 2017 Category: International Medicine & Public Health Authors: Alanoud Almuhareb, Zakaria Habib Source Type: research

Fatal invasive aspergillosis caused by Aspergillus niger after bilateral lung transplantation
Publication date: Available online 18 May 2017 Source:Medical Mycology Case Reports Author(s): Enora Atchade, Sylvain Jean-Baptiste, Sandrine Houze, Claire Chabut, Laurent Massias, Yves Castier, Olivier Brugière, Hervé Mal, Philippe Montravers Aspergillus niger is usually considered to be a low virulence fungus, not commonly reported to cause invasive infections. Invasive pulmonary aspergillosis due to Aspergillus niger was diagnosed in a 43-year-old woman following bilateral lung transplantation. Intravenous voriconazole failed to control progression of the disease. Despite salvage therapy with a combinatio...
Source: Medical Mycology Case Reports - May 19, 2017 Category: Biology Source Type: research

Species distribution and antifungal susceptibility patterns of Candida isolates from a public tertiary teaching hospital in the Eastern Cape Province, South Africa.
This study aimed to identify the species distribution of Candida isolates and to determine their unique antifungal susceptibility and resistance patterns. During a cross-sectional study, 209 Candida isolates (recovered from 206 clinical samples) were collected and their species distribution was determined using ChromAgar Candida. The Vitek-2 system (Biomerieux, South Africa) was used to determine minimum inhibitory concentrations (MICs) to azoles (fluconazole, voriconazole), echinocandins (caspofungin, micafungin), polyenes (amphotericin B) and flucytosine. Four species of Candida were isolated, of which C. albicans was th...
Source: Brazilian Journal of Medical and Biological Research - May 18, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Coexistence of aspergilloma and pulmonary hydatid cyst in an immunocompetent individual
Publication date: Available online 16 May 2017 Source:Journal de Mycologie Médicale / Journal of Medical Mycology Author(s): F. Aala, H. Badali, S. Hashemi Fesharaki, M. Boroumand, M. Sotoudeh Anvari, H. Davari, S. Agha Kuchak Afshari, S. Khodavaisy Echinococcosis is a zoonotic disease caused by Echinococcus granulosus sensu lato. The liver and lungs are the most commonly sites of infections, but involvements of other organs were also observed. Recently, the coinfection of pulmonary hydatid cyst with aspergilloma has been reported in the literature. Herein, we report a successful treatment of coinfection of cystic ...
Source: Journal of Medical Mycology - May 17, 2017 Category: Biology Source Type: research

Low penetration of caspofungin into cerebrospinal fluid following intravenous administration of standard doses
• Caspofungin (CAS) shows low capacity to penetrate into the human central nervous system (CNS).• In inflamed meninges, penetration of CAS may be increased in some patients.• Standard doses of CAS are not sufficient to treat fungal CNS infections. (Source: International Journal of Antimicrobial Agents)
Source: International Journal of Antimicrobial Agents - May 17, 2017 Category: Drugs & Pharmacology Authors: Volker Strenger, Fedja Farowski, Carsten M üller, Nora Hofer, Hans Jürgen Dornbusch, Daniela Sperl, Herwig Lackner, Martin Benesch, Christian Urban Tags: Short Communication Source Type: research

Sensitivity of White and Opaque Candida albicans to Antifungal Drugs.
Abstract White and opaque cells of Candida albicans have the same genome but differ in gene expression patterns, metabolic profiles, and host niche preferences. We tested whether these differences (which include the differential expression of drug transporters) resulted in different sensitivities to 27 antifungal agents. The analysis was performed in two different strain backgrounds; although there was strain-to-strain variation, only terbinafine hydrochloride and caspofungin showed consistent, two-fold differences between white and opaque cells across both strains. PMID: 28507115 [PubMed - as supplied by pub...
Source: Antimicrobial Agents and Chemotherapy - May 15, 2017 Category: Microbiology Authors: Craik VB, Johnson AD, Lohse MB Tags: Antimicrob Agents Chemother Source Type: research

Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species.
CONCLUSION: Although all of Candida isolates were susceptible to fluconazole in vitro, it should be used with caution for empirical therapy due to more resistant rates in clinic. In addition, due to valuable sensitivity of all tested strains to caspofungin, it potentially can be presented as the first line therapy for Candida vaginitis. PMID: 28491253 [PubMed - in process] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - May 12, 2017 Category: Microbiology Tags: Iran J Microbiol Source Type: research

Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates.
CONCLUSION: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. PMID: 28491252 [PubMed - in process] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - May 12, 2017 Category: Microbiology Tags: Iran J Microbiol Source Type: research

Endomyocardial and pericardial aspergillosis in critically ill patients
The objective of this study is to describe the clinical characteristics of histologically proven endomyocardial and pericardial invasion, in the context of IA, in critically ill patients. Eight critically ill patients with histopathological confirmation of endomyocardial/pericardial aspergillosis were evaluated. Risk factors, clinical and laboratory characteristics, treatment, histopathological characteristics and mortality were recorded. Signs and symptoms of cardiac dysfunction were not observed in any of the patients. Therapy was administered to six of them shortly after the first positive culture. The observed histopat...
Source: Mycoses - May 12, 2017 Category: Research Authors: George Dimopoulos, Frantzeska Frantzeskaki, Marinos Kosmopoulos, Fabio Sylvio Taccone, Anne ‐Marie Van den Abeele, Pierre Bulpa, Frederic Forêt, Dirk Vogelaers, Stijn Blot, Tags: ORIGINAL ARTICLE Source Type: research

Hepatotoxicity of Antimycotics Used for Invasive Fungal Infections: In Vitro Results.
Conclusion. Antimycotics, especially azoles, used for systemic infections should be given with caution in patient with liver insufficiency or liver failure or high risk for this; therefore, therapeutic drug monitoring should be used. Further studies with this approach are encouraged. PMID: 28473992 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 8, 2017 Category: Research Authors: Doß S, Potschka H, Doß F, Mitzner S, Sauer M Tags: Biomed Res Int Source Type: research

Luliconazole, an alternative antifungal agent against Aspergillus terreus
This study demonstrated that luliconazole had an excellent in vitro activity against all tested isolates of A. terreus, with MICGM 0.00236μg/mL than other tested antifungals. As a result, luliconazole could be a possible alternative antifungal for the treatment of aspergillosis due to A. terreus. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - May 6, 2017 Category: Biology Source Type: research

Antifungal activity of caspofungin in experimental infective endocarditis caused by Candida albicans.
CONCLUSIONS: The antifungal activity of caspofungin is similar to that of liposomal amphotericin B in an experimental model of infective endocarditis caused by C. albicans. PMID: 28443984 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 28, 2017 Category: Infectious Diseases Authors: Victorio GB, Bourdon LMB, Benavides LG, Huerta-Olvera SG, Plascencia A, Villanueva J, Martinez-Lopez E, Hernández-Cañaveral II Tags: Mem Inst Oswaldo Cruz Source Type: research

Efficacy of anidulafungin in 539 patients with invasive candidiasis: a patient-level pooled analysis of six clinical trials
Conclusions: These data demonstrate that anidulafungin is effective for treatment of candidaemia and invasive candidiasis in a broad patient population. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - April 28, 2017 Category: Microbiology Source Type: research

[Molecular identification and in vitro antifungal susceptibility of blood isolates of the Candida parapsilosis species complex in Venezuela].
CONCLUSIONS: The results obtained in Venezuela provide for the first time important information about the distribution of C. parapsilosis species complex in cases of candidaemia, and support the need for continuing surveillance programs, including molecular discrimination of species and antifungal susceptibility tests, which may guide specific therapy. PMID: 28457646 [PubMed - as supplied by publisher] (Source: Revista Iberoamericana de Micologia)
Source: Revista Iberoamericana de Micologia - April 27, 2017 Category: Biology Authors: Moreno X, Reviakina V, Panizo MM, Ferrara G, García N, Alarcón V, Garcés MF, Dolande M Tags: Rev Iberoam Micol Source Type: research

Comparison of Efficacy and Safety of Caspofungin Versus Micafungin in Pediatric Allogeneic Stem Cell Transplant Recipients: A Retrospective Analysis
ConclusionThese results demonstrate good efficacy and tolerability for caspofungin and micafungin. However, better results with respect to the incidence and resolution of fever in the micafungin group may suggest its use in preference to that of caspofungin. (Source: Advances in Therapy)
Source: Advances in Therapy - April 20, 2017 Category: Drugs & Pharmacology Source Type: research

Assessment of high-priced systemic antifungal prescriptions.
CONCLUSION: The overall incidence of appropriate use was higher in 2012 compared with 2007 (62% and 37% respectively, P=0.004). Nevertheless, costly antifungal prescriptions need to be optimized in particular for empirical therapy, dosage adjustment, and potential de-escalation to fluconazole. PMID: 28412043 [PubMed - as supplied by publisher] (Source: Medecine et Maladies Infectieuses)
Source: Medecine et Maladies Infectieuses - April 18, 2017 Category: Infectious Diseases Tags: Med Mal Infect Source Type: research

Drug interactions and safety profiles with concomitant use of caspofungin and calcineurin inhibitors in allogeneic hematopoietic cell transplantation
ConclusionsOur data showed that CPFG did not affect the pharmacokinetics of TAC and that it could mildly increase CsA blood concentrations in allo‐HCT patients. (Source: British Journal of Clinical Pharmacology)
Source: British Journal of Clinical Pharmacology - April 1, 2017 Category: Drugs & Pharmacology Authors: Mitsutaka Nishimoto, Hideo Koh, Atsushi Tokuwame, Yousuke Makuuchi, Masatomo Kuno, Teruhito Takakuwa, Hiroshi Okamura, Shiro Koh, Takuro Yoshimura, Satoru Nanno, Mika Nakamae, Asao Hirose, Yasuhiro Nakashima, Takahiko Nakane, Masayuki Hino, Hirohisa Nakam Tags: DRUG INTERACTIONS Source Type: research

Activity of exogenous tyrosol in combination with caspofungin and micafungin against Candida parapsilosis sessile cells
Abstract AimsFungal quorum sensing molecules may have an inhibitory effect as adjuvant against Candida biofilms. Therefore, in vitro activity of caspofungin and micafungin was evaluated against Candida parapsilosis biofilms in the presence of tyrosol. Methods and ResultsInteractions were assessed using fractional inhibitory concentration index (FICI) determination, metabolic activity‐based time‐kill experiments, and scanning electron microscopy. Tyrosol caused 1‐ to 16‐fold and 2‐ to 32‐fold decrease in median caspofungin and micafungin MICs, respectively. Based on FICI, synergy was observed in isolates 27001 a...
Source: Journal of Applied Microbiology - March 21, 2017 Category: Microbiology Authors: Ren átó Kovács, Zoltán Tóth, Fruzsina Nagy, Lajos Daróczi, Aliz Bozó, László Majoros Tags: Original Article Source Type: research

In vitro inhibitory effects of farnesol and interactions between farnesol and antifungals against biofilms of Candida albicans resistant strains.
Abstract Antifungal resistance is a serious problem in clinical infections. Farnesol, which is a potential antifungal agent against biofilms formed by Candida albicans resistant strains (a fluconazole-resistant isolate derived from SC5314 and two clinical Candida resistant isolates), was investigated in this study. The inhibitory effects of farnesol on biofilms were examined by XTT assay. The morphological changes and biofilm thicknesses were analyzed by scanning electron microscopy and confocal laser scanning microscopy, respectively. Additionally, the checkerboard microdilution method was used to investigate the...
Source: Biofouling - March 19, 2017 Category: Molecular Biology Authors: Xia J, Qian F, Xu W, Zhang Z, Wei X Tags: Biofouling Source Type: research

Dihydroorotate dehydrogenase inhibitor F901318 has potent in vitro activity against Scedosporium species and Lomentospora prolificans
Conclusions: F901318 demonstrated potentin vitro activity againstScedosporium species andL. prolificans. This activity was maintained against isolates that had significantly reduced susceptibility to the other antifungals. Further studies are warranted to evaluate thein vivo efficacy of F901318 againstScedosporium species andL. prolificans. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - March 15, 2017 Category: Microbiology Source Type: research

[Fungemia and septic arthritis caused by Saprochaete capitata in a patient with fanconi aplastic anemia: a case report].
In conclusion, S.capitata infections should be taken into consideration in the presence of fungemia and septic arthritis in hematological patients who receive caspofungin therapy. PMID: 28283014 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - March 12, 2017 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

Genetic diversity and in vitro antifungal susceptibility of 200 clinical and environmental Aspergillus flavus isolates.
In conclusion, posaconazole and anidulafungin showed the greatest in vitro activity among systemic azoles and echinocandins, respectively. However, majority of A. flavus isolates showed reduced susceptibility to amphotericin B. Antifungal susceptibility of A. flavus was not linked with the clinical or environmental source of isolation. Microsatellite genotyping may suggest an association between clinical and environmental strains, although this requires further investigation. PMID: 28264849 [PubMed - as supplied by publisher] (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - March 5, 2017 Category: Microbiology Authors: Taghizadeh-Armaki M, Hedayati MT, Ansari S, Mahdavi Omran S, Saber S, Rafati H, Zoll J, van der Lee HA, Melchers WJ, Verweij PE, Seyedmousavi S Tags: Antimicrob Agents Chemother Source Type: research

Predisposing factors and outcome of uncommon yeast species-related fungaemia based on an exhaustive surveillance programme (2002 –14)
Conclusions: UYS encompass a high diversity of species, each with its own behaviour and predisposing factors for human infections. This variety makes it important to rapidly identify an isolate to the species level in order to optimize antifungal treatment. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - March 4, 2017 Category: Microbiology Source Type: research

Biofilms of Candida spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?
ConclusionsThe results demonstrate that Candida from the ocular conjunctiva of horses can pose as a risk to animal health as they are capable of forming biofilms, which are commonly involved in fungal keratitis. (Source: Veterinary Ophthalmology)
Source: Veterinary Ophthalmology - March 1, 2017 Category: Veterinary Research Authors: Raimunda S âmia Nogueira Brilhante, Paula Vago Bittencourt, Débora Souza Collares Castelo‐Branco, Glaucia Morgana Melo Guedes, Jonathas Sales Oliveira, Lucas Pereira Alencar, Rossana Aguiar Cordeiro, Mariana Pinheiro, Evilázio Fernandes Nogueira‐Fi Tags: Original Article Source Type: research

Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus.
ch WJ Abstract Aspergillus fumigatus, the main etiological agent of invasive aspergillosis, is a leading cause of death in immunocompromised patients. Septins, a conserved family of GTP-binding proteins, serve as scaffolding proteins to recruit enzymes and key regulators to different cellular compartments. Deletion of the A. fumigatus septin aspB increases susceptibility to the echinocandin antifungal caspofungin. However, how AspB mediates this response to caspofungin is unknown. Here, we characterized the AspB interactome under basal conditions and after exposure to a clinically relevant concentration of ca...
Source: Biochemical and Biophysical Research communications - February 23, 2017 Category: Biochemistry Authors: Vargas-Muñiz JM, Renshaw H, Waitt G, Soderblom EJ, Moseley MA, Palmer JM, Juvvadi PR, Keller NP, Steinbach WJ Tags: Biochem Biophys Res Commun Source Type: research

Catheter-related Saccharomyces cerevisiae Fungemia Following Saccharomyces boulardii Probiotic Treatment: In a Child in Intensive Care Unit and Review of the Literature
We present the case of an 8-year-old patient in pediatric surgical intensive care unit who developed S. cerevisiae fungemia following probiotic treatment containing S. boulardii. Caspofungin was not effective in this case and he was treated with amphotericin B. We want to emphasize that physicians should be careful about probiotic usage in critically ill patients. (Source: Medical Mycology Case Reports)
Source: Medical Mycology Case Reports - February 21, 2017 Category: Biology Source Type: research

Multilocus Phylogeny and Antifungal Susceptibility of Aspergillus Section Circumdati from Clinical Samples and Description of A. pseudosclerotiorum sp. nov. [Mycology]
A multilocus phylogenetic study was carried out to assess species identity of a set of 34 clinical isolates from Aspergillus section Circumdati from the United States and to determine their in vitro antifungal susceptibility against eight antifungal drugs. The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) genes. The drugs tested were amphotericin B, itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, and terbinafine. The most common species sampled was A. we...
Source: Journal of Clinical Microbiology - February 21, 2017 Category: Microbiology Authors: Siqueira, J. P. Z., Sutton, D. A., Gene, J., Garcia, D., Wiederhold, N., Peterson, S. W., Guarro, J. Tags: Mycology Source Type: research

Tolerance to caspofungin in Candida albicans is associated with at least three distinctive mechanisms that govern expression of FKS genes and cell wall remodeling.
Abstract Expanding echinocandin use to prevent or treat invasive fungal infections has led to an increase in the number of breakthrough infections due to resistant Candida species. Although uncommon, echinocandin resistance is well documented for Candida albicans, which is among the most prevalent bloodstream organisms. A better understanding is needed to assess the cellular factors that promote tolerance and predispose infecting cells for clinical breakthrough. We have previously showed that some mutants adapted to growth in the presence of toxic sorbose due to loss of one chromosome 5 (Ch5) also became more tole...
Source: Antimicrobial Agents and Chemotherapy - February 20, 2017 Category: Microbiology Authors: Yang F, Zhang L, Wakabayashi H, Myers J, Jiang Y, Cao Y, Jimenez-Ortigosa C, Perlin DS, Rustchenko E Tags: Antimicrob Agents Chemother Source Type: research

Candida auris candidaemia in Indian ICUs: analysis of risk factors
Objectives: To identify the risk factors associated withCandida auris candidaemia, as this fungus now poses a global threat.Methods: We performed a subgroup analysis of a previously reported study of 27 Indian ICUs. The clinical data of candidaemia cases due toC. auris and otherCandida species were compared to determine significant risk factors associated withC. auris infection.Results: Of the 1400 candidaemia cases reported earlier, 74 (5.3%) from 19 of 27 ICUs were due toC. auris. The duration of ICU stay prior to candidaemia diagnosis was significantly longer in patients withC. auris candidaemia (median 25, IQR 12 &ndas...
Source: Journal of Antimicrobial Chemotherapy - February 20, 2017 Category: Microbiology Source Type: research

Fungicidal activity and PK/PD of caspofungin as tools to guide antifungal therapy in a fluconazole-resistant C. parapsilosis candidemia.
Authors: Tascini C, Sozio E, Di Paolo A, Tintori G, Leonildi A, Bertolino G, Carmassi F, Tagliaferri E, Menichetti F, Barchiesi F Abstract Candida parapsilosis may be responsible for bloodstream infections (BSI) and it is characterised by an increased incidence of fluconazole resistance. A 75-year old woman with severe comorbidities received the insertion of a peripherally inserted central venous catheter. Fluconazole did not prevent a C. parapsilosis BSI hence caspofungin was started after a nephrotoxic first-line treatment with amphotericin B. The ratio of peak plasma concentration over the minimum inhibitory con...
Source: Journal of Chemotherapy - February 19, 2017 Category: Cancer & Oncology Tags: J Chemother Source Type: research

Evaluation of synergistic anticandidal and apoptotic effects of ferulic acid and caspofungin against Candida albicans
This study aimed to investigate the synergy between anticandidal and apoptotic effects of ferulic acid and caspofungin against Candida albicans and Candida glabrata, with the help of a quantitative checkerboard microdilution assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as a viability dye. Apoptotic effects of caspofungin and ferulic acid concentrations (alone and combined) were analyzed for C. albicans and C. glabrata based on annexin V–propidium iodide binding capacities using flow cytometric analysis. C. albicans showed a synergistic effect, represented by a fractional inhibitory c...
Source: Journal of Food and Drug Analysis - February 12, 2017 Category: Food Science Source Type: research

Consistent high prevalence of Exophiala dermatitidis, a neurotropic opportunist, on railway sleepers
We examined 150 railway samples using cotton swabs moistened with sterile physiological saline. Black yeasts and relatives were selected on theirs colony morphology and identified based on ITS rDNA sequencing. Overall, 36 (24%) of samples were positive for black yeast-like fungi, i.e., Exophiala dermatitidis (n =20, 55.6%) was predominant, followed by E. phaeomuriformis (n =9, 25%), E. heteromorpha (n =5, 13.9%), and E. xenobiotica (n =2, 5.6%). Massive contaminations of E. dermatitidis were seen on railway sleepers on creosoted oak wood at the region close to the sea level, while in cold climates were ...
Source: Journal of Medical Mycology - February 9, 2017 Category: Biology Source Type: research

Persistent Candida conglobata bloodstream infection in a preterm neonate successfully treated by combination therapy with amphotericin B and caspofungin
Publication date: Available online 9 February 2017 Source:Journal de Mycologie Médicale / Journal of Medical Mycology Author(s): N. Al-Sweih, S. Ahmad, S. Khan, Z. Khan, L. Joseph, S. Vayalil, R. Chandy Fungemia due to uncommon/rare Candida species is an emerging problem of global clinical significance. Here, we describe a case of Candida conglobata bloodstream infection in a preterm neonate. The diagnosis was established by repeated isolation of C. conglobata in blood cultures and by detection of rDNA of the fungus in serum samples. The identity of the isolate as C. conglobata was confirmed by sequencing...
Source: Journal of Medical Mycology - February 8, 2017 Category: Biology Source Type: research

Successful treatment with caspofungin of candiduria in a child with Wilms tumor; review of literature
In conclusion, predisposing factors for better outcome are more important than treatment of CUTI, therefore, management of UTI is essential for critically patients. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - February 7, 2017 Category: Biology Source Type: research

New developments and directions in the clinical application of the echinocandins.
Abstract The echinocandins-caspofungin, anidulafungin and micafungin-are semi-synthetic cyclic hexapeptide antimicrobial agents with modified N-linked acyl lipid side chains which anchor the compounds to the phospholipid bilayer of the fungal cell membrane, thereby inhibiting synthesis of fungal cell wall glucan. Over the last 10 years, echinocandins have become the first-line antifungal treatment of candidaemia and other forms of invasive candidiasis (IC). Echinocandins are generally well tolerated, but their use is limited by their requirement for daily intravenous dosing, lack of oral formulation and limit...
Source: Archives of Toxicology - February 7, 2017 Category: Toxicology Authors: Chang CC, Slavin MA, Chen SC Tags: Arch Toxicol Source Type: research

Fulminant Laryngeal-tracheobronchial-pulmonary Aspergillosis: A Rare and Fatal Complication in Allogeneic Hematopoietic Stem Cell Transplantation Recipients.
Authors: Tao T, Zhang YH, Xue SL, Wu DP, Chen F Abstract A 23-year-old man who had previously undergone allogeneic hematopoietic stem cell transplantation (allo-HSCT) for severe aplastic anemia was diagnosed with invasive laryngeal-tracheobronchial-pulmonary aspergillosis after presenting with a persistent dry cough at six months post-transplantation based on the findings of laryngoscopy and fiberoptic bronchoscopy. A fiberoptic bronchoscope was used to remove the obstructive material from the patient's airway and posaconazole plus caspofungin were administered to successfully to treat the patient. Our report sugge...
Source: Internal Medicine - February 6, 2017 Category: Internal Medicine Tags: Intern Med Source Type: research

Corrigendum to "Caspofungin salvage therapy in Pneumocystis jirovecii pneumonia" [J Microbiol Immunol Infect (2016)] pii: S1684-1182(16)30029-9. [Epub ahead of print].
Corrigendum to "Caspofungin salvage therapy in Pneumocystis jirovecii pneumonia" [J Microbiol Immunol Infect (2016)] pii: S1684-1182(16)30029-9. [Epub ahead of print]. J Microbiol Immunol Infect. 2017 Feb 06;: Authors: Lee WS, Hsueh PR, Hsieh TC, Chen FL, Ou TY, Jean SS PMID: 28185780 [PubMed - as supplied by publisher] (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - February 5, 2017 Category: Microbiology Authors: Lee WS, Hsueh PR, Hsieh TC, Chen FL, Ou TY, Jean SS Tags: J Microbiol Immunol Infect Source Type: research

In vitro activity of the novel echinocandin CD101 at pH 7 and 4 against Candida spp. isolates from patients with vulvovaginal candidiasis
Conclusions: CD101 had potent activity against allCandida isolates tested, including azole-resistant strains. Although there was some reduction in activity at pH 4 versus 7, the resulting MIC values were still well below the intravaginal CD101 drug concentrations anticipated to be present following topical administration. These results support continued development of topical CD101 for the treatment of VVC/RVVC. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - February 3, 2017 Category: Microbiology Source Type: research

Invasive infections due to Saprochaete and Geotrichum species: Report of 23 cases from the FungiScope Registry
Summary Saprochaete and Geotrichum spp. are rare emerging fungi causing invasive fungal diseases in immunosuppressed patients and scarce evidence is available for treatment decisions. Among 505 cases of rare IFD from the FungiScope™ registry, we identified 23 cases of invasive infections caused by these fungi reported from 10 countries over a 12‐year period. All cases were adults and previous chemotherapy with associated neutropenia was the most common co‐morbidity. Fungaemia was confirmed in 14 (61%) cases and deep organ involvement included lungs, liver, spleen, central nervous system and kidneys. Fungi were S....
Source: Mycoses - January 31, 2017 Category: Research Authors: Luisa Dur án Graeff, Danila Seidel, Maria J. G. T. Vehreschild, Axel Hamprecht, Anupma Kindo, Zdenek Racil, Judit Demeter, Sybren De Hoog, Ute Aurbach, Maren Ziegler, Hilmar Wisplinghoff, Oliver A. Cornely, Tags: ORIGINAL ARTICLE Source Type: research

Species diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazil
Summary The Exophiala genus is responsible for many superficial and invasive infections resulting from black fungi. Identification of Exophiala at the species level is based on morphological observations complemented by molecular tests. The aim of this study was to identify 23 clinical isolates of Exophiala spp. and evaluate the antifungal susceptibility to seven different agents. Molecular identification was based on an analysis of ITS region of rDNA using genomic databases. The micromorphology was evaluated by microculture and scanning electron microscopy. The susceptibility tests were performed using the antifungal agen...
Source: Mycoses - January 31, 2017 Category: Research Authors: Wendy C. Silva, Sarah S. Gon çalves, Daniel W. C. L. Santos, Ana Carolina B. Padovan, Fernando C. Bizerra, Analy S. A. Melo Tags: ORIGINAL ARTICLE Source Type: research

Epidemiological investigation of Candida species causing bloodstream infection in paediatric small bowel transplant recipients
Summary Small bowel transplantation (SBT) can be a life‐saving medical procedure. However, these recipients experience high risk of bloodstream infections caused by Candida. This research aims to characterise the SBT recipient gut microbiota over time following transplantation and investigate the epidemiology of candidaemia in seven paediatric patients. Candida species from the recipients' ileum and bloodstream were identified by internal transcribed spacer sequence and distinguished to strain by multilocus sequence typing and randomly amplified polymorphic DNA. Antifungal susceptibility of bloodstream isolates was deter...
Source: Mycoses - January 30, 2017 Category: Research Authors: Mallory J. Suhr, Jo ão Carlos Gomes‐Neto, Nabaraj Banjara, Diana F. Florescu, David F. Mercer, Peter C. Iwen, Heather E. Hallen‐Adams Tags: ORIGINAL ARTICLE Source Type: research

Optimal doses of caspofungin during continuous venovenous hemodiafiltration in critically ill patients
(Source: Critical Care)
Source: Critical Care - January 30, 2017 Category: Intensive Care Authors: Gerardo Aguilar, Rafael Ferriols, Angels Lozano, Carlos Ezquer, Jos é A. Carbonell, Ana Jurado, Juan Carrizo, Ferran Serralta, Jaume Puig, David Navarro, Manuel Alos and F. Javier Belda Source Type: research

[A rare and mortal infection agent in patients with hematological malignancies: Saprochaete capitata].
In conclusion, S.capitata should be considered as an infection agent with high mortality risk in the neutropenic patients with hematologic malignancies, especially in the presence of persistent fever during the use of caspofungin. PMID: 28124967 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - January 28, 2017 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

First itraconazole resistant Aspergillus fumigatus clinical isolate harbouring a G54E substitution in Cyp51Ap in South America.
CONCLUSIONS: This is the first report in South America of a clinical A. fumigatus strain carrying the substitution G54E at Cyp51Ap associated with itraconazole resistance. Considering the patient was azole-naive, this resistant isolate may have been acquired from the environment. PMID: 28087293 [PubMed - as supplied by publisher] (Source: Revista Iberoamericana de Micologia)
Source: Revista Iberoamericana de Micologia - January 9, 2017 Category: Biology Authors: Leonardelli F, Theill L, Nardin ME, Macedo D, Dudiuk C, Mendez E, Gamarra S, Garcia-Effron G Tags: Rev Iberoam Micol Source Type: research

SCY-078 is Fungicidal in Time-Kill Studies Against Candida Species.
Abstract SCY-078 is an orally bioavailable ß-1,3-glucan synthesis inhibitor (GSI) and the first-in-class of structurally novel triterpine antifungals in clinical development for treating candidemia and invasive candidiasis. In vitro susceptibility by broth micro-dilution, antifungal carry-over, and time-kill dynamics were determined for 3 reference (ATCC) strains (C. albicans 90028, C. parapsilosis 90018, and C. tropicalis 750), a Quality Control (QC) strain (C krusei 6258), and 4 other strains (C. albicans MYA-2732, 64124, 76485 and C.glabrata 90030). Caspofungin (CASP), fluconazole (FLC), and voriconazole ...
Source: Antimicrobial Agents and Chemotherapy - January 8, 2017 Category: Microbiology Authors: Scorneaux B, Angulo D, Borroto-Esoda K, Ghannoum M, Peel M, Wring S Tags: Antimicrob Agents Chemother Source Type: research