Buruli Ulcer and Medical Geo-Microbiology
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - November 25, 2022 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research

Identification of potential candidate vaccines against Mycobacterium ulcerans based on the major facilitator superfamily transporter protein
This study aimed to generate a multi-epitope vaccine against M. ulcerans from the major facilitator superfamily transporter protein using an immunoinformatics approach. Twelve M. ulcerans genome assemblies were analyzed, resulting in the identification of 11 CD8+ and 7 CD4+ T-cell epitopes and 2 B-cell epitopes. These conserved epitopes were computationally predicted to be antigenic, immunogenic, non-allergenic, and non-toxic. The CD4+ T-cell epitopes were capable of inducing interferon-gamma and interleukin-4. They successfully bound to their respective human leukocyte antigens alleles in in silico docking studies. The ex...
Source: Frontiers in Immunology - November 8, 2022 Category: Allergy & Immunology Source Type: research

A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
by Estelle Marion, Numfor Hycenth, Sundeep Chaitanya Vedithi, Marie Robbe-Saule, Val érie Donkeng, Line-Marlène Ganlonon, Affolabi Dissou, Solange Kakou Ngazoa, Marie-Jose Kabedi, Arsène Mabika Mabika, Richard Phillips, Michael Frimpong, Dorothy Yeboah-Manu, Vera Yatta Walker, Olaoluwa Akinwale, Maman Issaka, Gisela Bretzel, Kinsgley Asiedu, Sara Eyangoh Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmentalMycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have report...
Source: PLoS Neglected Tropical Diseases - November 4, 2022 Category: Tropical Medicine Authors: Estelle Marion Source Type: research

Can Membrane Composition Traffic Toxins? Mycolactone and Preferential Membrane Interactions
Biophys J. 2022 Oct 17:S0006-3495(22)00857-8. doi: 10.1016/j.bpj.2022.10.019. Online ahead of print.ABSTRACTMycolactone is a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans and the sole causative agent of the neglected tropical skin disease Buruli ulcer. The toxin acts by invading host cells and interacting with intracellular targets to disrupt multiple fundamental cellular processes. Mycolactone's amphiphilic nature enables strong interactions with lipophilic environments, including cellular membranes; however, the specificity of these interactions and the role of membranes in the toxin's path...
Source: Biophysical Journal - October 19, 2022 Category: Physics Authors: Gabriel C A da Hora John D M Nguyen Jessica M J Swanson Source Type: research

Can Membrane Composition Traffic Toxins? Mycolactone and Preferential Membrane Interactions
Biophys J. 2022 Oct 17:S0006-3495(22)00857-8. doi: 10.1016/j.bpj.2022.10.019. Online ahead of print.ABSTRACTMycolactone is a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans and the sole causative agent of the neglected tropical skin disease Buruli ulcer. The toxin acts by invading host cells and interacting with intracellular targets to disrupt multiple fundamental cellular processes. Mycolactone's amphiphilic nature enables strong interactions with lipophilic environments, including cellular membranes; however, the specificity of these interactions and the role of membranes in the toxin's path...
Source: Biophysical Journal - October 19, 2022 Category: Physics Authors: Gabriel C A da Hora John D M Nguyen Jessica M J Swanson Source Type: research

Skin wounds in a rural setting of C ôte d’Ivoire: Population-based assessment of the burden and clinical epidemiology
ConclusionsSkin wounds are highly prevalent in rural West Africa, where they represent a widely neglected problem. The HDSS-based survey with combined active and passive case finding adopted in this study provides a better estimate than school- and health institution-based surveys which underestimate the frequency of skin wounds and, particularly, of neglected tropical diseases of the skin, such as Buruli ulcer and yaws. A comparison with country-specific WHO data suggests underreporting of Buruli ulcer cases. Trial registrationRegistration at ClinicalTrials.gov NCT03957447. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - October 13, 2022 Category: Tropical Medicine Authors: Simone Toppino Source Type: research

Patient Care and Treatment Strategies for Skin Diseases in Sub Saharan Africa: Role of Traditional and Western Medicines
This study comprises numerous research that sought to examine how the general public felt about the SSA's current healthcare system. In this review, common skin conditions such atopic dermatitis, buruli ulcers, dermatophytosis, and scabies were addressed. According to this report, government agencies must pay particular attention to skin illnesses in SSA and raise public awareness. Availability of medical care, socioeconomic factors, degree of education, and other factors influence patient attitudes toward traditional and western health care differently in different geographic areas. Facts suggested that self-medication is...
Source: Infectious Disorders Drug Targets - September 19, 2022 Category: Infectious Diseases Authors: Astha Sharma Sonali Sundram Rishabha Malviya Swati Verma Neeraj Kumar Fuloria Shivkanya Fuloria Mahendran Sekar Neelesh Mishra Dhanalekshmi Unnikrishnan Meenakshi Source Type: research

Patient Care and Treatment Strategies for Skin Diseases in Sub Saharan Africa: Role of Traditional and Western Medicines
This study comprises numerous research that sought to examine how the general public felt about the SSA's current healthcare system. In this review, common skin conditions such atopic dermatitis, buruli ulcers, dermatophytosis, and scabies were addressed. According to this report, government agencies must pay particular attention to skin illnesses in SSA and raise public awareness. Availability of medical care, socioeconomic factors, degree of education, and other factors influence patient attitudes toward traditional and western health care differently in different geographic areas. Facts suggested that self-medication is...
Source: Infectious Disorders Drug Targets - September 19, 2022 Category: Infectious Diseases Authors: Astha Sharma Sonali Sundram Rishabha Malviya Swati Verma Neeraj Kumar Fuloria Shivkanya Fuloria Mahendran Sekar Neelesh Mishra Dhanalekshmi Unnikrishnan Meenakshi Source Type: research

Environmental risk factors associated with the presence of < i > Mycobacterium ulcerans < /i > in Victoria, Australia
by Kim R. Blasdell, Bridgette McNamara, Daniel P. O ’Brien, Mary Tachedjian, Victoria Boyd, Michael Dunn, Peter T. Mee, Simone Clayton, Julie Gaburro, Ina Smith, Katherine B. Gibney, Ee Laine Tay, Emma C. Hobbs, Nilakshi Waidyatillake, Stacey E. Lynch, Timothy P. Stinear, Eugene Athan In recent years reported cases of Buruli ulcer, caused byMycobacterium ulcerans, have increased substantially in Victoria, Australia, with the epidemic also expanding geographically. To develop an understanding of howM.ulcerans circulates in the environment and transmits to humans we analyzed environmental samples collected from 115 proper...
Source: PLoS One - September 13, 2022 Category: Biomedical Science Authors: Kim R. Blasdell Source Type: research

Quantifying Population Burden and Effectiveness of Decentralized Surveillance Strategies for Skin-Presenting Neglected Tropical Diseases, Liberia
Emerg Infect Dis. 2022 Sep;28(9):1755-1764. doi: 10.3201/eid2809.212126.ABSTRACTWe evaluated programmatic approaches for skin neglected tropical disease (NTD) surveillance and completed a robust estimation of the burden of skin NTDs endemic to West Africa (Buruli ulcer, leprosy, lymphatic filariasis morbidity, and yaws). In Maryland, Liberia, exhaustive case finding by community health workers of 56,285 persons across 92 clusters identified 3,241 suspected cases. A total of 236 skin NTDs (34.0 [95% CI 29.1-38.9]/10,000 persons) were confirmed by midlevel healthcare workers trained using a tailored program. Cases showed a f...
Source: Emerging Infectious Diseases - August 23, 2022 Category: Infectious Diseases Authors: Joseph W S Timothy Emerson Rogers Katherine E Halliday Tarnue Mulbah Michael Marks Zeela Zaizay Romeo Giddings Marie Kempf Estelle Marion Stephen L Walker Karsor K Kollie Rachel L Pullan Source Type: research

Quantifying Population Burden and Effectiveness of Decentralized Surveillance Strategies for Skin-Presenting Neglected Tropical Diseases, Liberia
Emerg Infect Dis. 2022 Sep;28(9):1755-1764. doi: 10.3201/eid2809.212126.ABSTRACTWe evaluated programmatic approaches for skin neglected tropical disease (NTD) surveillance and completed a robust estimation of the burden of skin NTDs endemic to West Africa (Buruli ulcer, leprosy, lymphatic filariasis morbidity, and yaws). In Maryland, Liberia, exhaustive case finding by community health workers of 56,285 persons across 92 clusters identified 3,241 suspected cases. A total of 236 skin NTDs (34.0 [95% CI 29.1-38.9]/10,000 persons) were confirmed by midlevel healthcare workers trained using a tailored program. Cases showed a f...
Source: Emerging Infectious Diseases - August 23, 2022 Category: Infectious Diseases Authors: Joseph W S Timothy Emerson Rogers Katherine E Halliday Tarnue Mulbah Michael Marks Zeela Zaizay Romeo Giddings Marie Kempf Estelle Marion Stephen L Walker Karsor K Kollie Rachel L Pullan Source Type: research

Genome-wide screening identified < i > SEC61A1 < /i > as an essential factor for mycolactone-dependent apoptosis in human premonocytic THP-1 cells
by Akira Kawashima, Mitsuo Kiriya, Junichiro En, Kazunari Tanigawa, Yasuhiro Nakamura, Yoko Fujiwara, Yuqian Luo, Keiji Maruyama, Shigekazu Watanabe, Masamichi Goto, Koichi Suzuki Buruli ulcer is a chronic skin disease caused by a toxic lipid mycolactone produced byMycobacterium ulcerans, which induces local skin tissue destruction and analgesia. However, the cytotoxicity pathway induced by mycolactone remains largely unknown. Here we investigated the mycolactone-induced cell death pathway by screening host factors using a genome-scale lenti-CRISPR mutagenesis assay in human premonocytic THP-1 cells. As a result, 884 gene...
Source: PLoS Neglected Tropical Diseases - August 8, 2022 Category: Tropical Medicine Authors: Akira Kawashima Source Type: research

Variable Number Tandem Repeat Profiling of Mycobacterium ulcerans Reveals New Genotypes in Buruli Ulcer Endemic Communities in Ghana and C ôte d’Ivoire
This study aimed at identifying genetic differences among M. ulcerans strains between two BU endemic countries: Ghana and Côte d’Ivoire. Clinical samples consisting of swabs, fine needle aspirates, and tissue biopsies of suspected BU lesions and environmental samples (e.g., water, biofilms from plants, soil, and detrital material) were analyzed. BU cases were confirmed via acid fast staining and PCR targeting the 16S rRNA, IS2404, IS2606, and ER domain genes present on M. ulcerans. Heterogeneity among M. ulcerans was determined through VNTR profiling targeting 10 loci. Eleven M. ulcerans genotypes were identified within...
Source: Frontiers in Microbiology - June 24, 2022 Category: Microbiology Source Type: research

What the snake leaves in its wake: Functional limitations and disabilities among snakebite victims in Ghanaian communities
ConclusionThe burden of snakebite in the community includes long-term disabilities of mild to moderate severity, which need to be considered when designing appropriate public health interventions. Estimating the total burden of snakebite is complicated by geographic differences in types of snakes and their clinical manifestations. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - May 23, 2022 Category: Tropical Medicine Authors: Leslie Mawuli Aglanu Source Type: research

Neglected tropical diseases in Australia: a narrative review
Med J Aust. 2022 May 12. doi: 10.5694/mja2.51533. Online ahead of print.ABSTRACT•Neglected tropical diseases (NTDs) represent a threat to the health, wellbeing and economic prosperity of billions of people worldwide, often causing serious disease or death. •Commonly considered diseases of low and middle-income nations, the presence of NTDs in high income countries such as Australia is often overlooked. •Seven of the 20 recognised NTDs are endemic in Australia: scabies, soil-transmitted helminths and strongyloidiasis, echinococcosis, Buruli ulcer, leprosy, trachoma, and snakebite envenoming. •Dengue, while not curre...
Source: Med J Aust - May 13, 2022 Category: General Medicine Authors: Johanna Kurcheid Catherine A Gordon Naomi E Clarke Kinley Wangdi Matthew Kelly Aparna Lal Polydor N Mutombo Dongxu Wang Mary L Mationg Archie Ca Clements Stephen Muhi Richard S Bradbury Beverley-Ann Biggs Wendy Page Gail Williams Donald P McManus Darren G Source Type: research