Normal pressure hydrocephalus secondary to Lyme disease, a case report and review of seven reported cases
Infection with tick borne Borrelia Burgdorferi (Lyme disease) can without treatment rarely develop into a chronic phase. Secondary Normal Pressure Hydrocephalus (sNPH) based on chronic infection with Borrelia ... (Source: BMC Neurology)
Source: BMC Neurology - September 16, 2020 Category: Neurology Authors: Louise N ørreslet Gimsing and Anne-Mette Hejl Tags: Case report Source Type: research

Detection of tick-borne pathogens in ticks from dogs and cats in Yamagata Prefecture, Japan, 2018.
Abstract Companion animals can become infected with tick-borne diseases (TBDs) and can become hosts of transmission to humans, thereby damaging human health. To clarify whether companion animals are infested by ticks harboring TBD pathogens in humans, we detected TBD pathogens in ticks collected from dogs and cats brought to animal hospitals in Yamagata Prefecture, Japan. Investigation of 164 adult ticks collected from 88 dogs and 41 cats during March-July 2018 revealed the predominant tick species as Ixodes ovatus (n = 95, 57.9%), followed by Ixodes nipponensis (37, 22.6%) and Haemaphysalis flava (10, 6.1%). The ...
Source: Japanese Journal of Infectious Diseases - August 31, 2020 Category: Infectious Diseases Authors: Seto J, Tanaka S, Kawabata H, Ito Y, Ikeda T, Mizuta K Tags: Jpn J Infect Dis Source Type: research

Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
Publication date: Available online 24 August 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Fernando S. Flores, María N. Saracho-Bottero, Patrick S. Sebastian, José M. Venzal, Atilio J. Mangold, Santiago Nava (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - August 26, 2020 Category: Zoology Source Type: research

A Case Report of Early Disseminated Lyme Disease
Preema Sinha, Bhavni Oberoi, Yadvendra S Sirohi, Aradhana Sood, Saikat BhattacharjeeNeurology India 2020 68(4):916-918 Lyme disease is a multi-organ infectious disease caused by the spirochete “Borrelia burgdorferi,” and transmitted by the “Ixodes” tick. Early disseminated Lyme disease can have varied central nervous system manifestations ranging from meningitis to radiculopathy and cranial neuropathy. If not suspected, misdiagnosis or delayed diagnosis can prove to be fatal. Erythema migrans is the most common clinical presentation, thereby, making dermatological examination extremely crucial in e...
Source: Neurology India - August 26, 2020 Category: Neurology Authors: Preema Sinha Bhavni Oberoi Yadvendra S Sirohi Aradhana Sood Saikat Bhattacharjee Source Type: research

Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis [Bacteriology]
Diagnosis of Lyme neuroborreliosis (LNB) is challenging, as long as Borrelia-specific intrathecal antibodies are not yet detectable. The chemokine CXCL13 is elevated in the cerebrospinal fluid (CSF) of LNB patients. Here, we compared the performances of the Euroimmun CXCL13 enzyme-linked immunosorbent assay (CXCL13 ELISA) and the ReaScan CXCL13 lateral flow immunoassay (CXCL13 LFA), a rapid point-of-care test, to support the diagnosis of LNB. In a dual-center case-control study, CSF samples from 90 patients (34 with definite LNB, 10 with possible LNB, and 46 with other central nervous system [CNS] diseases [non-LNB group])...
Source: Journal of Clinical Microbiology - August 24, 2020 Category: Microbiology Authors: Ziegler, K., Rath, A., Schoerner, C., Meyer, R., Bertsch, T., Erbguth, F., Bogdan, C., Steinmann, J., Held, J. Tags: Bacteriology Source Type: research

Complement evasion strategies of Borrelia burgdorferi sensu lato
Borreliosis (Lyme disease) is a spirochetal disease caused by the species complex ofBorrelia  burgdorferi transmitted byIxodes spp. ticks. Recorded to be the most common tick ‐borne disease in the world, the last two decades have seen an increase in disease incidence and distribution, exceeding 360 000 cases in Europe alone. If untreated, infection may cause skin symptoms, arthritis, and neurological or cardiac complications. Borrelia spirochetes have developed strate gies to evade the mammalian host immune system. These include the complement system, which is an important first‐line defense mechanism against...
Source: FEBS Letters - August 23, 2020 Category: Biochemistry Authors: Vinaya Dulipati, Seppo Meri, Jaana Panelius Tags: Review Article Source Type: research

Lyme Borreliosis with Scalp Eschar Mimicking Rickettsial Infection, Austria.
We report on a patient in Austria with scalp eschar and neck lymphadenopathy. Rickettsial etiology was excluded by culture, PCR, and serologic tests. Borrelia afzelii was identified from the eschar swab by PCR. Lyme borreliosis can mimic rickettsiosis; appropriate tests should be included in the diagnostic workup of patients with eschars. PMID: 32818412 [PubMed - in process] (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - August 22, 2020 Category: Infectious Diseases Authors: Markowicz M, Schötta AM, Wijnveld M, Stanek G Tags: Emerg Infect Dis Source Type: research

Human Borrelia miyamotoi Infection, Austria.
We report a human case of Borrelia miyamotoi infection diagnosed in Austria. Spirochetes were detected in Giemsa-stained blood smears. The presence of B. miyamotoi in the patient's blood was confirmed by PCR, and phylogenetic analysis identified an infection with a strain from Europe. PMID: 32818401 [PubMed - in process] (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - August 22, 2020 Category: Infectious Diseases Authors: Tobudic S, Burgmann H, Stanek G, Winkler S, Schötta AM, Obermüller M, Markowicz M, Lagler H Tags: Emerg Infect Dis Source Type: research

Isoforskolin and Cucurbitacin IIa promote the expression of anti-inflammatory regulatory factor SIGIRR in human macrophages stimulated with Borrelia burgdorferi basic membrane protein A.
In conclusion, These results reveal new possibilities for the treatment of Lyme arthritis. PMID: 32829087 [PubMed - as supplied by publisher] (Source: International Immunopharmacology)
Source: International Immunopharmacology - August 20, 2020 Category: Allergy & Immunology Authors: Peng Y, Chen T, Luo L, Li L, Cao W, Xu X, Zhang Y, Yue P, Dai X, Ji Z, Jian M, Bai R, Ding Z, Wang F, Wen S, Zhou G, Kong J, Luo S, Liu A, Bao F Tags: Int Immunopharmacol Source Type: research

Response to Weiss MF re: “Seroprevalence of Borrelia IgM and IgG Antibodies in Healthy Individuals: A Caution Against Serology Misinterpretations and Unnecessary Antibiotic Treatments”
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - August 20, 2020 Category: Infectious Diseases Authors: Zuzana Strizova Daniel Smrz Jirina Bartunkova Source Type: research

En kvinne i 50- årene med residiverende svimmelhet.
En kvinne i 50-årene med residiverende svimmelhet. Tidsskr Nor Laegeforen. 2020 Aug 18;140(11): Authors: Eriksen E, Smebye KL, Mejlænder-Evjensvold M, Sandset EC Abstract BACKGROUND: Vasculitis caused by neuroborreliosis is one of many rare described causes of stroke. CASE PRESENTATION: A female smoker in her fifties presented with dizziness due to a left cerebellar infarction, and after general stroke workup atherosclerosis was considered the probable cause. In the preceding months she had experienced nonspecific intermittent headache, neck pain and dizziness initially attributed to ...
Source: Tidsskrift for den Norske Laegeforening - August 18, 2020 Category: General Medicine Authors: Eriksen E, Smebye KL, Mejlænder-Evjensvold M, Sandset EC Tags: Tidsskr Nor Laegeforen Source Type: research

Development of A Loop-Mediated Isothermal Amplification (LAMP) Assay for Detection of Relapsing Fever Borreliae.
Conclusion: The high sensitivity and specificity of the test, along with the simplicity of the DNA extraction procedure, make the LAMP a reliable and adaptable tool for the diagnosis of tick-borne relapsing fever in rural endemic areas. PMID: 32766348 [PubMed] (Source: Iranian Journal of Arthropod-Borne Diseases)
Source: Iranian Journal of Arthropod-Borne Diseases - August 13, 2020 Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research

Changes in renal parameters and their association with subclinical vector-borne infections in Bernese Mountain dogs
An increased risk for glomerulonephritis and a higher prevalence of antibodies to Borrelia (B.) burgdorferi sensu lato have been reported in Bernese mountain dogs (BMDs). The aim of the study was to determine the... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - August 12, 2020 Category: Veterinary Research Authors: C. Prey ß-Jägeler, K. Hartmann and R. Dorsch Tags: Research article Source Type: research

Detection of Asian-Type Borrelia miyamotoi from Ixodes ricinus Inhabiting Tver Province (Russia): A Sympatric Region for I. ricinus and Ixodes persulcatus
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - August 6, 2020 Category: Infectious Diseases Authors: Toshiyuki Masuzawa Keiko Sakakibara Kazushi Suzuki Hiroki Sato Shima Yasuda Source Type: research

Complement evasion strategies of Borrelia burgdorferi sensu lato
AbstractBorreliosis (Lyme disease) is a spirochetal disease caused by the species complex ofBorrelia burgdorferi transmitted byIxodes spp. ticks. Recorded to be the most common tick ‐borne disease in the world, the last two decades have seen an increase in disease incidence and distribution, exceeding 360 000 cases in Europe alone. If untreated, infection may cause skin symptoms, arthritis, neurological or cardiac complications. Borrelia spirochetes have developed strategies to evade the mammalian host immune system. These include the complement system, which is an important first‐line defence mechanism against invadin...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: V Dulipati, S Meri, J Panelius Tags: REVIEW Source Type: research

Detection of Leishmania infantum DNA and antibodies against Anaplasma spp., Borrelia burgdorferi s.l. and Ehrlichia canis in a dog kennel in South-Central Romania
This study aimed to asse... (Source: Acta Veterinaria Scandinavica)
Source: Acta Veterinaria Scandinavica - August 3, 2020 Category: Veterinary Research Authors: Cristina Daniela Cazan, Angela Monica Ionic ă, Ioana Adriana Matei, Gianluca D’Amico, Clara Muñoz, Eduardo Berriatua and Mirabela Oana Dumitrache Tags: Brief communication Source Type: research

Re: “Seroprevalence of Borrelia IgM and IgG Antibodies in Healthy Individuals: A Caution Against Serology Misinterpretations and Unnecessary Antibiotic Treatments” by Strizova et al.
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - August 3, 2020 Category: Infectious Diseases Authors: Miriam F. Weiss Source Type: research

Microbiomes and Specific Symbionts of Social Spiders: Compositional Patterns in Host Species, Populations, and Nests
Social spiders have remarkably low species-wide genetic diversities, potentially increasing the relative importance of microbial symbionts for host fitness. Here we explore the bacterial microbiomes of three species of social Stegodyphus (S. dumicola, S. mimosarum, and S. sarasinorum), within and between populations, using 16S rRNA gene amplicon sequencing. The microbiomes of the three spider species were distinct but shared similarities in membership and structure. This included low overall diversity (Shannon index 0.5–1.7), strong dominance of single symbionts in individual spiders (McNaughton’s dominance ind...
Source: Frontiers in Microbiology - July 31, 2020 Category: Microbiology Source Type: research

Human Borrelia miyamotoi Infection, Austria
S. Tobudic et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - July 29, 2020 Category: Infectious Diseases Source Type: research

Molecular Detection of a Borrelia sp. in Nymphs of Amblyomma brasiliense Ticks (Acari: Ixodidae) from Iguaçu National Park, Brazil, Genetically Related to Borrelia from Ethiopia and Côte D’Ivoire
Publication date: Available online 25 July 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Camila Alves dos Santos, Adriane Suzin, Alexandre Vogliotti, Pablo Henrique Nunes, Amália Regina Mar Barbieri, Marcelo B. Labruna, Matias Pablo Juan Szabó, Jonny Yokosawa (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - July 26, 2020 Category: Zoology Source Type: research

Update on the presence of Ixodes ricinus at the western limit of its range and the prevalence of Borrelia burgdorferi sensu lato
Publication date: Available online 24 July 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Annetta Zintl, Taher Zaid, Fiona McKiernan, Amalia Naranjo-Lucena, Jeremy Gray, Sorcha Brosnan, John Browne, Jack O’Connor, John Mee, Barbara Good, Emma L. Gillingham, Alexander G.C. Vaux, Jolyon Medlock (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - July 24, 2020 Category: Zoology Source Type: research

Immune Evasion Strategies of Relapsing Fever Spirochetes
Relapsing fever (RF) is claimed a neglected arthropod-borne disease caused by a number of diverse human pathogenic Borrelia (B.) species. These RF borreliae are separated into the groups of tick-transmitted species including B. duttonii, B. hermsii, B. parkeri, B. turicatae, B. hispanica, B. persica, B. caucasica, and B. myiamotoi, and the louse-borne Borrelia species B. recurrentis. As typical blood-borne pathogens achieving high cell concentrations in human blood, RF borreliae (RFB) must outwit innate immunity, in particular complement as the first line of defense. One prominent strategy developed by RFB to evade innate ...
Source: Frontiers in Immunology - July 23, 2020 Category: Allergy & Immunology Source Type: research

GSE153261 The Lyme disease bacterium, Borrelia burgdorferi, stimulates an inflammatory response in human choroid plexus epithelial cells
We report the results of gene expression profiling via RNA-sequencing of primary human choroid plexus epithelial cells in vitro that were infected with Borrelia burgdorferi, the causative agent of Lyme disease. Epithelial cells were cultured with B. burgdorferi for 48 hours prior to RNA isolation. Following high-throughput sequencing via Illumina HiSeq 4000, a total of 258 differentially expressed genes were identified. Subsequent data analysis indicates an inflammatory and immune response generated by the epithelial cells following infection, marked by chemotactic cytokines and interferon stimulated genes. Several genes a...
Source: GEO: Gene Expression Omnibus - July 10, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Seropositivity for pathogens associated with chronic infections is a risk factor for all-cause mortality in the elderly: findings from the Memory and Morbidity in Augsburg Elderly (MEMO) Study
We examined the effect of a positive serostatus at baseline for selected pathogens associated with chronic infections (Helicobacter pylori,Borrelia burgdorferi sensulato,Toxoplasma gondii, cytomegalovirus, Epstein-Barr virus, herpes simplex virus 1/2, and human herpesvirus 6) on all-cause mortality with multivariable parametric survival models. We found a reduced survival time in individuals with a positive serostatus forHelicobacter pylori (accelerated failure time (AFT) − 15.92, 95% CI − 29.96; − 1.88), cytomegalovirus (AFT − 22.81, 95% CI − 36.41; −&...
Source: AGE - July 9, 2020 Category: Geriatrics Source Type: research

In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography.
Abstract Spirochetes such as Treponema, Borrelia, and Leptospira species can rotate their bodies to swim in liquid environments by rotating periplasmic flagella or endoflagella, which are present inside the cell. Electron cryotomography (ECT) is an imaging technique that directly provides three-dimensional (3D) structures of cells and molecular complexes in their cellular environment at nanometer resolution. Here, I present a general protocol of ECT that covers the sample preparation, data collection, tilt series alignment, and tomographic reconstruction for visualization of intact periplasmic flagella in Leptospi...
Source: Mol Biol Cell - July 8, 2020 Category: Molecular Biology Authors: Kawamoto A Tags: Methods Mol Biol Source Type: research

Antibody profiling of a Borrelia burgdorferi C6 antibody positive, clinically affected Rottweiler and her pups
Publication date: Available online 5 July 2020Source: The Veterinary JournalAuthor(s): A.L. Hatke, D.R. Green, K. Stasiak, R.T. Marconi (Source: The Veterinary Journal)
Source: The Veterinary Journal - July 7, 2020 Category: Veterinary Research Source Type: research

Molecular Detection of Leptospira interrogans and Borrelia burgdorferi in Wild Rodents from Mexico
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - July 7, 2020 Category: Infectious Diseases Authors: Jorge J. Rodr íguez-Rojas Ángel Rodríguez-Moreno Rosa M. S ánchez-Casas Jes ús J. Hernández-Escareño Source Type: research

Performance of a Modified Two-Tiered Testing Enzyme Immunoassay Algorithm for Serologic Diagnosis of Lyme Disease in Nova Scotia [Immunoassays]
Compared to the standard two-tiered testing (STTT) algorithm for Lyme disease serology using an enzyme immunoassay (EIA) followed by Western blotting, data from the United States suggest that a modified two-tiered testing (MTTT) algorithm employing two EIAs has improved sensitivity to detect early localized Borrelia burgdorferi infections without compromising specificity. From 2011 to 2014, in the Canadian province of Nova Scotia, where Lyme disease is hyperendemic, sera submitted for Lyme disease testing were subjected to a whole-cell EIA, followed by C6 EIA and subsequently IgM and/or IgG immunoblots on sera with EIA-pos...
Source: Journal of Clinical Microbiology - June 24, 2020 Category: Microbiology Authors: Davis, I. R. C., McNeil, S. A., Allen, W., MacKinnon-Cameron, D., Lindsay, L. R., Bernat, K., Dibernardo, A., LeBlanc, J. J., Hatchette, T. F. Tags: Immunoassays Source Type: research

Characterization of a Borrelia miyamotoi membrane antigen (BmaA) for serodiagnosis of Borrelia miyamotoi disease
Publication date: Available online 31 May 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Emma K. Harris, Kevin S. Brandt, Taylor J. Van Gundy, Irina Goodrich, Gary P. Wormser, Brittany A. Armstrong, Robert D. Gilmore (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - June 1, 2020 Category: Zoology Source Type: research

Presence of Borrelia miyamotoi infection in a highly endemic area of Lyme disease
A series of cases in the Northeast of the US during 2013 –2015 described a new Borrelia species, Borrelia miyamotoi, which is transmitted by the same tick species that transmits Lyme disease and causes a relapsing... (Source: Annals of Clinical Microbiology and Antimicrobials)
Source: Annals of Clinical Microbiology and Antimicrobials - May 30, 2020 Category: Microbiology Authors: Luis A. Marcos, Kalie Smith, Kelsey Reardon, Fredric Weinbaum and Eric D. Spitzer Tags: Short report Source Type: research

Crystallization and X-ray analysis of Borrelia burgdorferi β -barrel assembly machinery A
Mitochondria, chloroplasts and several species of bacteria have outer membrane proteins (OMPs) that perform many essential biological functions. The β -barrel assembly machinery (BAM) complex is one of the OMPs of Borrelia burgdorferi, the pathogenic spirochete that causes Lyme disease, and its BamA component (BbBamA) includes a C-terminal β -barrel domain and five N-terminal periplasmic polypeptide-transport-associated (POTRA) domains, which together perform a central transport function. In the current work, the production, crystallization and X-ray analysis of the three N-terminal POTRA domains of BbBamA (BbBam...
Source: Acta Crystallographica Section F - May 29, 2020 Category: Biochemistry Authors: Dong, S. Chu, H. Wen, K. Yu, Q. Li, H. Wang, C. Qin, X. Tags: Borrelia burgdorferi BbBamA-POTRA P1 – P3 crystallization research communications Source Type: research

The Lyme Disease Biobank: Characterization of 550 Patient and Control Samples from the East Coast and Upper Midwest of the United States [Immunoassays]
Lyme disease (LD) is an increasing public health problem. Current laboratory testing is insensitive in early infection, the stage at which appropriate treatment is most effective in preventing disease sequelae. The Lyme Disease Biobank (LDB) collects samples from individuals with symptoms consistent with early LD presenting with or without erythema migrans (EM) or an annular, expanding skin lesion and uninfected individuals from areas of endemicity. Samples were collected from 550 participants (298 cases and 252 controls) according to institutional review board-approved protocols and shipped to a centralized biorepository....
Source: Journal of Clinical Microbiology - May 26, 2020 Category: Microbiology Authors: Horn, E. J., Dempsey, G., Schotthoefer, A. M., Prisco, U. L., McArdle, M., Gervasi, S. S., Golightly, M., De Luca, C., Evans, M., Pritt, B. S., Theel, E. S., Iyer, R., Liveris, D., Wang, G., Goldstein, D., Schwartz, I. Tags: Immunoassays Source Type: research

IJERPH, Vol. 17, Pages 3607: Local Population Structure and Seasonal Variability of Borrelia garinii Genotypes in Ixodes ricinus Ticks, Slovakia
In this study, multilocus sequence typing (MLST) was employed to investigate the local population structure of B. garinii in Ixodes ricinus ticks. The study took place in a natural wetland in Slovakia, temporally encompassing spring and autumn bird migration periods as well as the breeding period of resident birds. In total, we examined 369 and 255 ticks collected from 78 birds and local vegetation, respectively. B. burgdorferi s.l. was detected in 43.4% (160/369) of ticks recovered from birds and in 26.3% (67/255) of questing ticks, respectively. Considering the ticks from bird hosts, the highest prevalence was found for ...
Source: International Journal of Environmental Research and Public Health - May 21, 2020 Category: Environmental Health Authors: Zuzana Mtierov á Mark éta Derdáková Michal Chvost áč Yuliya M. Didyk Barbara Mangov á Veronika Rus ňáková Tarageľová Diana Selyemov á Al žbeta Šujanová Radovan V áclav Tags: Article Source Type: research

Keds, the enigmatic flies and their role as vectors of pathogens.
Abstract Hippoboscid flies (Diptera: Hippoboscidae), commonly known as keds or louse flies, have been for long time overlooked by the scientific community, and their vector role of infectious agents to humans and domestic animals has been scantly investigated. This is partly due to the fact that the host range for most species is primarily restricted to wildlife, being rarely reported on domestic animals and humans. This led to a scarce scientific knowledge about their biology, ecology, behaviour, epidemiology as well as vector competence. However, the life history of some hippoboscid species, e.g., Melophagus ovi...
Source: Acta Tropica - May 21, 2020 Category: Infectious Diseases Authors: Bezerra-Santos MA, Otranto D Tags: Acta Trop Source Type: research

The Lon-1 Protease Is Required by Borrelia burgdorferi To Infect the Mammalian Host [Molecular Pathogenesis]
Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. In addition, B. burgdorferi encodes a second Lon homolog called Lon-1. Recent studies suggest that Lon-1 may function differently from the prototypical Lon protease. However, the function of Lon-1 in B. burgdorferi biology remains virtually unknown. Particularly, the contribution of Lon-1 to B. burgdorferi fitness and infection remains hitherto unexplored. Herein, we show that Lon-1 plays a critical role for the infection of B. burgdorferi in a mammalian host. We found that lon-1 was highly expressed during animal infection, implying ...
Source: Infection and Immunity - May 20, 2020 Category: Infectious Diseases Authors: Thompson, C., Mason, C., Parrilla, S., Ouyang, Z. Tags: Molecular Pathogenesis Source Type: research

Immunoserological Diagnosis of Human Borrelioses: Current Knowledge and Perspectives
Spirochetes of the genus Borrelia are divided into relapsing fever borreliae and Lyme disease borreliae. Immunoserological assays have been poorly developed for relapsing fever borreliae, where direct detection methods are more adapted to the pathophysiology of these infections presenting with massive bacteraemia. However, emergence of the novel agent of relapsing fever B. miyamotoi has renewed interest in serology in this context. In Lyme disease, because direct detection methods show low sensitivity, serology plays a central role in the diagnostic strategy. This diagnostic strategy is based on a two-tier methodology invo...
Source: Frontiers in cellular and infection microbiology - May 19, 2020 Category: Microbiology Source Type: research

Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En fran çais : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse).
Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En français : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse). J Obstet Gynaecol Can. 2020 May;42(5):644-653 Authors: Smith GN, Moore KM, Hatchette TF, Nicholson J, Bowie W, Langley JM Abstract OBJECTIVE: Lyme disease is an emerging infection in Canada caused by the bacterium belonging to the Borrelia burgdorferi sensu lato species complex, which is transmitted via the bite of an infected blacklegged tick. Populations of blacklegged ticks continue to expand and are now es...
Source: Journal of Obstetrics and Gynaecology Canada : JOGC - May 18, 2020 Category: OBGYN Tags: J Obstet Gynaecol Can Source Type: research

Ticks, fleas and rodent-hosts analyzed for the presence of Borrelia miyamotoi in Slovakia: the first record of Borrelia miyamotoi in a Haemaphysalis inermis tick
Publication date: Available online 16 May 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Ivana Heglasová, Natalie Rudenko, Maryna Golovchenko, Dana Zubriková, Dana Miklisová, Michal Stanko (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - May 17, 2020 Category: Zoology Source Type: research

Bacterial infection and non-Hodgkin's lymphoma.
ult D Abstract One quarter of all cancers are linked to infectious diseases. The link between viral infection and cancer has been widely studied, but few reports have focused on the carcinogenic role of bacterial infection. Nonetheless, Helicobacter pylori, Chlamydia psittaci, Coxiella burnetii, Borrelia burgdorferi and Campylobacter jejuni are bacteria that can be associated with non-Hodgkin's lymphoma (NHL), the most common haematologic malignancy. Here, we review the evidence in favour of a link between these bacterial infections and NHL. Sero-epidemiological observation makes it possible to identify a link bet...
Source: Critical Reviews in Microbiology - May 15, 2020 Category: Microbiology Authors: Melenotte C, Mezouar S, Mège JL, Gorvel JP, Kroemer G, Raoult D Tags: Crit Rev Microbiol Source Type: research

Tinidazole: Another Therapeutic Option for Syphilis?
Abstract After the incidental observation of an almost complete resolution of maculopapular eruption in a patient having simultaneously secondary syphilis and trichomonas vaginalis infection, we extended the treatment with tinidazole (500 mg 4 times daily for 7 days) to 10 other early syphilis patients before the start of the conventional penicillin treatment. All patients showed marked improvement of their lesions in a few days. After the introduction of the conventional penicillin regimen, the lesions further improved and VDRL titers declined at least 4-fold within 6 months in all patients. Tinidazole is a 5-nit...
Source: The Journal of Clinical Pharmacology - May 14, 2020 Category: Drugs & Pharmacology Authors: Drago F, Ciccarese G, Rebora A, Parodi A Tags: J Clin Pharmacol Source Type: research

Seroprevalence of Borrelia IgM and IgG Antibodies in Healthy Individuals: A Caution Against Serology Misinterpretations and Unnecessary Antibiotic Treatments
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - May 12, 2020 Category: Infectious Diseases Authors: Zuzana Strizova Daniel Smrz Jirina Bartunkova Source Type: research

Modified two-tiered testing algorithm for Lyme disease serology: the Canadian context.
Conclusion: Diagnostic improvements in sensitivity of LD testing without significant loss of specificity have been consistently reported when MTTT is compared with STTT in studies conducted in highly LD endemic regions. Our working group agrees with the recommendation by the United States Centers for Disease Control that serological testing for LD using MTTT is an acceptable alternative to STTT. This recommendation is contingent on development and implementation of comprehensive validation studies on the performance of MTTT vs STTT within the Canadian context, including evaluation of the test performance in areas of low en...
Source: Can Commun Dis Rep - May 7, 2020 Category: Infectious Diseases Tags: Can Commun Dis Rep Source Type: research

"Candidatus Borrelia ibitipoquensis," a Borrelia valaisiana-Related Genospecies Characterized from Ixodes paranaensis in Brazil.
"Candidatus Borrelia ibitipoquensis," a Borrelia valaisiana-Related Genospecies Characterized from Ixodes paranaensis in Brazil. Microb Ecol. 2020 May 04;: Authors: Muñoz-Leal S, Ramirez DG, Luz HR, Faccini JLH, Labruna MB Abstract Borrelia burgdorferi sensu lato (Bbsl) spirochetes include the agents of Lyme borreliosis in temperate regions of the Northern Hemisphere, and merge their transmission cycles mainly with ticks of the Ixodes ricinus complex. Twenty genospecies compose Bbsl currently, and with the exception of Borrelia chilensis, and Borrelia garinii, all have been described ...
Source: Microbial Ecology - May 4, 2020 Category: Microbiology Authors: Muñoz-Leal S, Ramirez DG, Luz HR, Faccini JLH, Labruna MB Tags: Microb Ecol Source Type: research

Identification of the proteins of Borrelia garinii interacting with human brain microvascular endothelial cells
Publication date: Available online 21 April 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Zuzana Tkáčová, Lucia Borszéková Pulzová, Evelína Mochnáčová, Irene Jiménez-Munguía, Katarína Bhide, Patrícia Mertinková, Petra Majerová, Amod Kulkarni, Andrej Kováč, Mangesh Bhide (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - April 23, 2020 Category: Zoology Source Type: research

A Fully Automated Multiplex Assay for Diagnosis of Lyme Disease with High Specificity and Improved Early Sensitivity [Immunoassays]
Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferi sensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi. The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in patients with early Lyme infection. Asymptomatic subjects from endemic and nonendemic origins demonstrated a seroreactivity rate of approximately 4% that was similar to other commercial assays evaluated in this study. Coupled to this result was the observation that the Lyme Total assay retained a high first-t...
Source: Journal of Clinical Microbiology - April 23, 2020 Category: Microbiology Authors: Hahm, J. B., Breneman, J. W., Liu, J., Rabkina, S., Zheng, W., Zhou, S., Walker, R. P., Kaul, R. Tags: Immunoassays Source Type: research

Epidemiological serosurvey of vector-borne and zoonotic pathogens among homeless people living in shelters in Marseille: cross-sectional one-day surveys (2005 –2015)
AbstractHomeless people are often exposed to unhygienic environments as well as to animals carrying arthropods which both transmit zoonotic infections and human louse-borne pathogens. We attempted to determine the prevalence of antibodies against several vector-borne and zoonotic pathogens among homeless adults living in Marseille. During the 2005 –2015 period, we collected sera samples from 821 homeless adults living in shelters. Antibodies againstBartonella quintana,Bartonella henselae,Borrelia recurrentis,Coxiella burnetii,Francisella tularensis (with a cut-off of 1:100),Rickettsia akari,Rickettsia conorii,Rickett...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2020 Category: Microbiology Source Type: research

A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation
In this study, we developed a new filter-based chemical extraction technique that allows measurement of high quality Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS. We used 49 isolates of 13 different species to produce the largest mass-library for Borrelia spp. so far and to validate the protocol. The library was successfully established and identifies>96% of used isolates correctly to species level. Cluster analysis on the sum spectra was applied to all the different isolates, which resulted in tight cluster generation for most species. Comparative analysis of the generated cluster to a...
Source: Frontiers in Microbiology - April 21, 2020 Category: Microbiology Source Type: research

The Factor H-Binding Site of CspZ as a Protective Target against Multistrain, Tick-Transmitted Lyme Disease [Microbial Immunity and Vaccines]
In this study, we demonstrated that immunization with CspZ-YA, a CspZ mutant protein with no FH-binding activity, protected mice from infection by several spirochete genotypes introduced via tick feeding. We found that the sera from CspZ-YA-vaccinated mice more efficiently eliminated spirochetes and blocked CspZ FH-binding activity than sera from CspZ-immunized mice. We also found that vaccination with CspZ, but not CspZ-YA, triggered the production of anti-FH antibodies, justifying CspZ-YA as an LD vaccine candidate. The mechanistic and efficacy information derived from this study provides insights into the development of...
Source: Infection and Immunity - April 20, 2020 Category: Infectious Diseases Authors: Marcinkiewicz, A. L., Lieknina, I., Yang, X., Lederman, P. L., Hart, T. M., Yates, J., Chen, W.-H., Bottazzi, M. E., Mantis, N. J., Kraiczy, P., Pal, U., Tars, K., Lin, Y.-P. Tags: Microbial Immunity and Vaccines Source Type: research

Assessment of Borrelia miyamotoi in febrile patients and ticks in Alsace, an endemic area for Lyme borreliosis in France
Borrelia miyamotoi is a relapsing fever Borrelia species transmitted by ticks of the Ixodes ricinus complex. Human disease caused by B. miyamotoi was first described in Russia and later in the USA and Japan. Addi... (Source: Parasites and Vectors)
Source: Parasites and Vectors - April 17, 2020 Category: Microbiology Authors: Pierre H. Boyer, Joris Koetsveld, Laurence Zilliox, Hein Sprong, Émilie Talagrand-Reboul, Yves Hansmann, Sylvie Josiane de Martino, Nathalie Boulanger, Joppe W. Hovius and Benoît Jaulhac Tags: Short report Source Type: research

Borrelia burgdorferi mimicking central nervous system relapse in diffuse large B cell lymphoma
(Source: Annals of Hematology)
Source: Annals of Hematology - April 15, 2020 Category: Hematology Source Type: research