Regulation of Synovial γδ T Cell Ligand Expression by Mitochondrial Reactive Oxygen Species and Gasdermin-D
J Immunol. 2022 Nov 23:ji2101166. doi: 10.4049/jimmunol.2101166. Online ahead of print.ABSTRACTγδ T cells reside at mucosal and epithelial barriers, and they often accumulate at sites of inflammation, both infectious and autoimmune, as well as in certain tumors. However, progress in understanding their function is considerably hampered by a lack of full understanding of the ligands recognized by TCR-γδ and how expression of these ligands is regulated. We recently developed a soluble human TCR-γδ (Vγ9Vδ1) tetramer from a synovial γδ T cell clone of a Lyme arthritis patient and observed that it stains monocytes act...
Source: Journal of Immunology - November 29, 2022 Category: Allergy & Immunology Authors: Cheryl C Collins Peter Hahn Zhaozhao Jiang Katherine A Fitzgerald Tsan Sam Xiao Ralph C Budd Source Type: research

Regulation of Synovial γδ T Cell Ligand Expression by Mitochondrial Reactive Oxygen Species and Gasdermin-D
J Immunol. 2022 Nov 23:ji2101166. doi: 10.4049/jimmunol.2101166. Online ahead of print.ABSTRACTγδ T cells reside at mucosal and epithelial barriers, and they often accumulate at sites of inflammation, both infectious and autoimmune, as well as in certain tumors. However, progress in understanding their function is considerably hampered by a lack of full understanding of the ligands recognized by TCR-γδ and how expression of these ligands is regulated. We recently developed a soluble human TCR-γδ (Vγ9Vδ1) tetramer from a synovial γδ T cell clone of a Lyme arthritis patient and observed that it stains monocytes act...
Source: Journal of Immunology - November 29, 2022 Category: Allergy & Immunology Authors: Cheryl C Collins Peter Hahn Zhaozhao Jiang Katherine A Fitzgerald Tsan Sam Xiao Ralph C Budd Source Type: research

Cas9-mediated endogenous plasmid loss in < i > Borrelia burgdorferi < /i >
by Constantin N. Takacs, Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner The spirocheteBorrelia burgdorferi, which causes Lyme disease, has the most segmented genome among known bacteria. In addition to a linear chromosome, theB.burgdorferi genome contains over 20 linear and circular endogenous plasmids. While many of these plasmids are dispensable under in vitro culture conditions, they are maintained during the natural life cycle of the pathogen. Plasmid-encoded functions are required for colonization of the tick vector, transmission to the vertebrate host, and evasion of host immune defenses. DifferentBorrelia s...
Source: PLoS One - November 28, 2022 Category: Biomedical Science Authors: Constantin N. Takacs Source Type: research

Differential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR
This study shows the differential presence of TBPs in samples of human platelet fraction (n = 68), whole blood samples (n = 68) and ticks collected (n = 76) from the same individuals, using an unbiased high-throughput pathogen detection microfluidic system. The clinical symptoms were characterized in enrolled patients. In patients with suspected TBP infection, serological assays were conducted to test for the presence of antibodies against specific TBPs. Tick species infesting humans were identified as Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis punctata. Eight patients developed local skin lesions at the si...
Source: Acta Tropica - November 26, 2022 Category: Infectious Diseases Authors: Pavle Banovi ć Elianne Piloto-Sardi ñas Dragana Mijatovi ć Ang élique Foucault-Simonin Verica Simin Ivana Bogdan Dasiel Obreg ón Lourdes Mateos-Hern ández Sara Moutailler Alejandro Cabezas-Cruz Source Type: research

Differential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR
This study shows the differential presence of TBPs in samples of human platelet fraction (n = 68), whole blood samples (n = 68) and ticks collected (n = 76) from the same individuals, using an unbiased high-throughput pathogen detection microfluidic system. The clinical symptoms were characterized in enrolled patients. In patients with suspected TBP infection, serological assays were conducted to test for the presence of antibodies against specific TBPs. Tick species infesting humans were identified as Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis punctata. Eight patients developed local skin lesions at the si...
Source: Acta Tropica - November 26, 2022 Category: Infectious Diseases Authors: Pavle Banovi ć Elianne Piloto-Sardi ñas Dragana Mijatovi ć Ang élique Foucault-Simonin Verica Simin Ivana Bogdan Dasiel Obreg ón Lourdes Mateos-Hern ández Sara Moutailler Alejandro Cabezas-Cruz Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005 ‒2020
Emerg Infect Dis. 2022 Dec;28(12):2389-2397. doi: 10.3201/eid2812.212612.ABSTRACTSince its initial identification in 1986, Lyme disease has been clinically diagnosed in 29 provinces in China; however, national incidence data are lacking. To summarize Lyme disease seropositivity data among persons across China, we conducted a systematic literature review of Chinese- and English-language journal articles published during 2005‒2020. According to 72 estimates that measured IgG by using a diagnostic enzyme-linked assay (EIA) alone, the seropositivity point prevalence with a fixed-effects model was 9.1%. A more conservative 2-...
Source: Emerging Infectious Diseases - November 23, 2022 Category: Infectious Diseases Authors: James H Stark Xiuyan Li Ji Chun Zhang Leah Burn Srinivas R Valluri Jiaxin Liang Kaijie Pan Mark A Fletcher Raphael Simon Luis Jodar Bradford D Gessner Source Type: research

Supportive Oligonucleotide Therapy (SOT) as a Potential Treatment for Viral Infections and Lyme Disease: Preliminary Results
This study revealed that for Lyme Disease, one or two SOT administrations can lead to a statistically significant decrease in DNA copies, while for viruses, two or three administrations are required to achieve a statistically significant reduction in the genetic material. These preliminary results indicate that antisense SOT therapy can be considered a potential treatment for viral as well as Lyme diseases.PMID:36412742 | DOI:10.3390/idr14060084 (Source: Herpes)
Source: Herpes - November 22, 2022 Category: Infectious Diseases Authors: Panagiotis Apostolou Aggelos Iliopoulos Georgios Beis Ioannis Papasotiriou Source Type: research

Use of the Western blot technique to identify the immunogenic proteins of Borrelia burgdorferi for developing a Lyme disease vaccine
CONCLUSION: The strong immune response to the 41 kDa flagellin protein by the different mammalian species suggests the utility of a possible vaccine targeting this protein, although other proteins may also be appropriate, for preventing Lyme disease following a bite from an infected tick.PMID:36403566 | DOI:10.1016/j.biopha.2022.114013 (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - November 20, 2022 Category: Drugs & Pharmacology Authors: Katherine Loomba Danilo Shi Tshering Sherpa Justin Chen Thomas J Daniels Charles S Pavia Dong Zhang Source Type: research

023 Tick feeding on human skin represses the local immune response facilitating pathogen transmission
As a consequence of global temperature rise, ticks (Ixodida) and tick-borne diseases are emerging. During tick attachment to human skin, the feeding cavity becomes a site of transmission for tick salivary compounds, which can exert immunosuppressive effects. Tick-borne pathogens including Borrelia burgdorferi may benefit from dampened immune activation at the bite site. We therefore assessed local and circulating human immune cells and cytokine secretion upon tick bite and developed a human skin explant model mimicking Ixodes ricinus bite. (Source: Journal of Investigative Dermatology)
Source: Journal of Investigative Dermatology - November 18, 2022 Category: Dermatology Authors: J. Strobl, V. M ündler, A. Gindl, A. Schötta, S. Müller, L. Kleissl, M. Markowicz, H. Stockinger, G. Stary Source Type: research

Increased macrophage phagocytic activity with TLR9 agonist conjugation of an anti- Borrelia burgdorferi monoclonal antibody
Clin Immunol. 2022 Nov 14:109180. doi: 10.1016/j.clim.2022.109180. Online ahead of print.ABSTRACTBorrelia burgdorferi (Bb) infection causes Lyme disease, for which there is need for more effective therapies. Here, we sequenced the antibody repertoire of plasmablasts in Bb-infected humans. We expressed recombinant monoclonal antibodies (mAbs) representing the identified plasmablast clonal families, and identified their binding specificities. Our recombinant anti-Bb mAbs exhibit a range of activity in mediating macrophage phagocytosis of Bb. To determine if we could increase the macrophage phagocytosis-promoting activity of ...
Source: Clinical Immunology - November 17, 2022 Category: Allergy & Immunology Authors: Shaghayegh Jahanbani Paige S Hansen Lisa K Blum Effie E Bastounis Nitya S Ramadoss Mallesh Pandrala Jessica Marie Kirschmann Grace Sisemore Blacker Zelda Z Love Irving L Weissman Fahimeh Nemati Michal Caspi Tal William H Robinson Source Type: research