Bioactive phytochemicals from the tubers of < em > Bletilla striata < /em > Rchb.f
Nat Prod Res. 2023 Sep 23:1-6. doi: 10.1080/14786419.2023.2261605. Online ahead of print.ABSTRACTThirty-four phytochemicals were isolated from dry tubers of Bletilla striata Rchb.f. The compounds were classified as bibenzyls 1-14, dihydrophenanthrenes 15, 17, 20, 21, phenanthrenes 16, 18, 19, simple benzoids 22-24, a fatty acid 25, glucosyloxybenzyl 2-isobutylmalates 26-32, and glucosyloxybenzyl cinnamates 33, 34. Compounds 1-4, 7, 8, 11, 12, and 16 inhibited melanogenesis (17.96-55.27%) induced by α-MSH in B16F10 cells at 10-40 μM. However, compounds 9, 10, 17, 18, and 21 exhibited significant cytotoxicity against melan...
Source: Natural Product Research - September 23, 2023 Category: Biochemistry Authors: Chia-Lin Lee Yun-Lian Jhan Hsiu-Mei Chiang Chao-Jung Chen Source Type: research

Microansamycins J and K from < em > Micromonospora < /em > sp. HK160111mas13OE
Nat Prod Res. 2023 Sep 23:1-5. doi: 10.1080/14786419.2023.2261606. Online ahead of print.ABSTRACTMicroansamycins were novel pentaketide ansamycins isolated from Micromonospora sp. HK160111mas13OE with AHBA-C2-C2-C3-C3 skeleton and diverse post-PKS modifications. In this paper, two new congeners, namely microansamycins J (1) and K (2), were identified based on their NMR, HRESIMS data and compared with those of microansamycins F and E. Neither showed antibacterial activity against Staphy-lococcus aureus ATCC25923 and Escherichia coli at 40 µg/mL.PMID:37740639 | DOI:10.1080/14786419.2023.2261606 (Source: Natural Product Research)
Source: Natural Product Research - September 23, 2023 Category: Biochemistry Authors: Jianxiong Wang Chunhua Lu Yajie Tang Yuemao Shen Source Type: research

UHPLC-MS characterisation of principal triterpene glycosides and biological activities of different solvent extracts of < em > Allochrusa gypsophiloides < /em > (Caryophyllaceae)
Nat Prod Res. 2023 Sep 23:1-5. doi: 10.1080/14786419.2023.2260068. Online ahead of print.ABSTRACTA crude methanol extract of the roots of Allochrusa gypsophiloides (syn. Acanthophyllum gypsophiloides) (collected from the Tashkent region of Uzbekistan) was chemically characterised by UHPLC-ESI-QTOF-MS/MS analysis. The results indicate the presence of six major bisdesmosidic saponins derived from gypsogenin, gypsogenic and quillaic acids, including five compounds reported for the first time for this species. The chloroform, methanol and water extracts of A. gypsophiloides showed weak antioxidant and anthelmintic activities. ...
Source: Natural Product Research - September 23, 2023 Category: Biochemistry Authors: Rano Mamadalieva Vaxobjon Xujayev Farukh S Sharopov Michael Wink Source Type: research

Characterisation analysis of bio oil generation using < em > Hibiscus cannabinus < /em > seeds
Nat Prod Res. 2023 Sep 23:1-9. doi: 10.1080/14786419.2023.2261142. Online ahead of print.ABSTRACTBio-oil from Hibiscus cannabinus seeds was extracted with four solvents: acetone, benzene, isopropanol, and hexane. Solvent extraction techniques have less oil residue than other methods. The primary factors that influence the extraction processes such as extraction time, particle size, seed ageing, and solvent suitability were optimised to enhance the bio-oil yield. The extracted bio-oil was tested by GC-MS, FTIR, and 1H-NMR. Physicochemical properties (free fatty acid, specific gravity, acid value, calorific value, saponifica...
Source: Natural Product Research - September 23, 2023 Category: Biochemistry Authors: Govindhan P Source Type: research

A new isoflavonoid derivative, evaluation of the antioxidant capacity and phenolic content of < em > Genista lobelii < /em > DC
Nat Prod Res. 2023 Sep 23:1-6. doi: 10.1080/14786419.2023.2261073. Online ahead of print.ABSTRACTThe phytochemical investigation of both chloroform and ethyl acetate extracts of Genista lobelii DC from an Algerian endemic plant led to the isolation of a new isoflavonoid Aspalathoidin compound (1) and nine known compounds (laburnetin, isokaempferide, retamasin B, ephedrodin, sakuretin, isoprunetin, genistein -7-O-β-glucoside, derrone-4'-O-methyl ether and eriodictyol-7,3'-dimethylether). Twelve phenolic compounds were identified in ethyl acetate and n-butanol extracts through a qualitative investigation of phenolic compoun...
Source: Natural Product Research - September 23, 2023 Category: Biochemistry Authors: Rabia Boukaabache Wafa Kerkatou Chaouki Bensouici Fadila Benayache Ouahiba Boumaza Source Type: research

Luteolin inhibits Porphyromonas gingivalis growth and alleviates alveolar bone destruction in experimental murine periodontitis
This study demonstrated luteolin in vitro and in vivo anti-bacterial activities. Thus, luteolin inhibits planktonic growth and biofilm formation in P. gingivalis. Furthermore, oral administration of luteolin alleviated maxillary alveolar bone resorption in murine periodontitis induced by P. gingivalis infection. These results indicate that luteolin may be a potential therapeutic compound that targets P. gingivalis by hindering its growth, biofilm formation, and alveolar bone resorption in the oral cavity.PMID:37740573 | DOI:10.1093/bbb/zbad137 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - September 23, 2023 Category: Biochemistry Authors: Toru Kariu Nobushiro Hamada Kesavalu Lakshmyya Source Type: research

A new approach to make scientific research more efficient – rethinking sustainability
As interest in sustainability grows, many researchers raise questions about changing scientific practices. To enable effective change, we reconceptualize sustainability as an approach that optimizes the efficiency of procedures, thereby benefiting scientists and minimizing environmental footprints. Since the implementation of sustainable approaches can be challenging, we describe the 6R concept as a framework to arrive at actionable steps. (Source: FEBS Letters)
Source: FEBS Letters - September 23, 2023 Category: Biochemistry Authors: Patrick Penndorf, Johannes Jabs Tags: The Scientists' Forum Source Type: research

Nifuroxazide repurposing for protection from diabetes ‐induced retinal injury in rats: Implication of oxidative stress and JAK/STAT3 axis
Nifuroxazide (Nifu), the potent STAT3 inhibitor successfully protected the diabetic rats against diabetic retinopathy as it ameliorated the retinal structure deterioration. This effect could be attributed to modulating JAK/STAT3 axis and oxidative stress. AbstractThe prevalence of diabetes mellitus (DM) is alarmingly increasing worldwide. Diabetic retinopathy (DR) is a prevailing DM microvascular complication, representing the major cause of blindness in working-age population. Inflammation is a crucial player in DR pathogenesis. JAK/STAT3 axis is a pleotropic cascade that modulates diverse inflammatory events. Nifuroxazid...
Source: BioFactors - September 23, 2023 Category: Biochemistry Authors: Nehal M. Elsherbiny, Reem Altemani, Waad Althagfi, Maha Albalawi, Zuhair M. Mohammedsaleh, Mohamed El ‐Sherbiny, Nada F. Abo El‐Magd Tags: RESEARCH ARTICLE Source Type: research

Osteoporosis in Adrenal Insufficiency: Could Metformin be Protective?
AbstractAdrenal insufficiency (AI) is a serious disorder characterized by the adrenal glucocorticoid deficiency. Regardless of the etiology, AI patients need long-term replacement therapy for glucocorticoids and, in some cases, for mineralocorticoids. The replacement therapy cannot completely mirror the physiological secretion patterns, and therefore, glucocorticoid excess is a common sequela in AI patients. Moreover, due to the absence of the reliable clinical markers to monitor the adequacy of the replacement therapy, clinicians often over-treat the AI patients to avoid adrenal crisis. Long-term glucocorticoid use is ass...
Source: Indian Journal of Clinical Biochemistry - September 23, 2023 Category: Biochemistry Source Type: research

Identification of an Alepterolic Acid Derivative as a Potent Anti-Breast-Cancer Agent via Inhibition of the Akt/p70S6K Signaling Pathway
Chem Biodivers. 2023 Sep 22:e202301248. doi: 10.1002/cbdv.202301248. Online ahead of print.ABSTRACTAlepterolic acid is a diterpene occurring in the fern Aleuritopteris argentea with potentially biological activity warrant further structural modification. In the present work, sixteen alepterolic acid derivatives were synthesized and evaluated for their anti-cancer activities. Among them, N-[m-(trifluoromethoxy)phenyl] alepterolamide displayed comparable activity (IC50=4.20±0.21 μM) in MCF-7 cells. Moreover, mechanistic investigations indicated this compound was significantly capable of diminishing cell proliferation and v...
Source: Chemistry and Biodiversity - September 22, 2023 Category: Biochemistry Authors: Guozheng Huang Nina Wang Lei Zhang Junjie Yu Kaili Chang Minghui Fan Zi Liu Liang Ma Jianguo Cao Source Type: research

Liking of salt is associated with depression, anxiety, and stress
Chem Senses. 2023 Sep 20:bjad038. doi: 10.1093/chemse/bjad038. Online ahead of print.ABSTRACTEarly research has shown variations in salt taste qualities in depression, anxiety, and stress. These studies evaluated changes to salt taste intensity and liking (pleasantness) of salt solutions but not of salty foods. Therefore, an Australian population survey (n = 424) was conducted where participants rated recalled intensity and liking of salt index foods and completed the Depression, Anxiety, and Stress Scale (DASS-21) to measure these states. Standard least squares regression (post hoc Tukey's HSD) compared means between grou...
Source: Chemical Senses - September 22, 2023 Category: Biochemistry Authors: Celeste Ferraris Christopher J Scarlett Tamara Bucher Emma L Beckett Source Type: research

Treatment target achievement in patients with familial hypercholesterolemia: a real-world descriptive study
CONCLUSION: Target achievement rate was unacceptably low in our FH patients. Conventional lipid-lowering treatments alone may not be sufficient in most FH patients to ensure adequate cardiovascular prevention.PMID:37739254 | DOI:10.1016/j.clinbiochem.2023.110649 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - September 22, 2023 Category: Biochemistry Authors: Simon Faubert Martine Paquette Alexis Baass Sophie Bernard Source Type: research

Toxic effect of Cd burden on the gut microflora and immune responses of wolf spider Pardosa pseudoannulata
This study provides insight into the underlying mechanisms of the gut microflora-immune system interaction of P. pseudoannulata in response to Cd burden.PMID:37739022 | DOI:10.1016/j.cbpc.2023.109747 (Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP)
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - September 22, 2023 Category: Biochemistry Authors: Juan Wang Xinru Zhang Yun'e Tang Zhi Wang Source Type: research

Inhibition of STIM1 alleviates high glucose-induced proliferation and fibrosis by inducing autophagy in mesangial cells
In this study, we investigated the role of STIM1 in the proliferation and fibrosis in high glucose (HG)-induced HBZY-1 cells. We found that the expression of STIM1 was increased in renal tissues of diabetic rat and HBZY-1 cells stimulated by HG. Downregulation of STIM1-mediated SOCE suppressed hyperglycemic cell proliferation and fibrosis by activating autophagy. In addition, the inhibitory effect of downregulating STIM1 on cells was blocked by autophagy inhibitor Bafilomycin A1 (BafA1). Moreover, this experiment also showed that STIM1 regulated autophagy, cell proliferation and fibrosis via PI3K/AKT/mTOR signal pathway. T...
Source: Molecular and Cellular Biochemistry - September 22, 2023 Category: Biochemistry Authors: Xixi Zeng Anbang Sun Weiyi Cheng Xin Hou Min Zhu Yanhong Liao Source Type: research

Evaluation of self-amplifying mRNA platform for protein expression and genetic stability: Implication for mRNA therapies
Biochem Biophys Res Commun. 2023 Sep 13;680:108-118. doi: 10.1016/j.bbrc.2023.09.016. Online ahead of print.ABSTRACTThe consecutive launch of mRNA vaccines like mRNA-1273, BNT 162b2, and GEMCOVAC®-19 against COVID-19 has triggered the debate of long-term expression, safety, and genomic integration of the mRNA vaccine platforms. In the present study, we examined the longevity of antigenic protein expression of mRNA-614 and mRNA-S1LC based on self-amplifying mRNA (SAM) in Expi-293F™, HEK-293 T, and ARPE-19 cells. The protein expression was checked by sandwich-ELISA, FACS, luciferase activity assay, and Western blot. The t...
Source: Biochemical and Biophysical Research communications - September 22, 2023 Category: Biochemistry Authors: Swarda Deo Kaushik Desai Aishwarya Patare Rucha Wadapurkar Saniya Rade Siddhi Mahudkar Madhura Sathe Shalini Srivastava Pragya Prasanna Ajay Singh Source Type: research