Tracking and Characterization of a Novel Conjugative Transposon Identified by Shotgun Transposon Mutagenesis
The horizontal transfer of mobile genetic elements (MGEs) is an essential process determining the functional and genomic diversity of bacterial populations. MGEs facilitate the exchange of fitness determinant genes like antibiotic resistance and virulence factors. Various computational methods exist to identify potential MGEs, but confirming their ability to transfer requires additional experimental approaches. Here, we apply a transposon (Tn) mutagenesis technique for confirming mobilization without the need for targeted mutations. Using this method, we identified two MGEs, including a previously known conjugative transpo...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Exposure to environmental toxicants is associated with gut microbiome dysbiosis, insulin resistance and obesity
Environ Int. 2024 Mar 20;186:108569. doi: 10.1016/j.envint.2024.108569. Online ahead of print.ABSTRACTEnvironmental toxicants (ETs) are associated with adverse health outcomes. Here we hypothesized that exposures to ETs are linked with obesity and insulin resistance partly through a dysbiotic gut microbiota and changes in the serum levels of secondary bile acids (BAs). Serum BAs, per- and polyfluoroalkyl substances (PFAS) and additional twenty-seven ETs were measured by mass spectrometry in 264 Danes (121 men and 143 women, aged 56.6 ± 7.3 years, BMI 29.7 ± 6.0 kg/m2) using a combination of targeted and suspect screening...
Source: Environment International - March 24, 2024 Category: Environmental Health Authors: Partho Sen Yong Fan Jennifer J Schlezinger Stanislav D Ehrlich Thomas F Webster Tuulia Hy ötyläinen Oluf Pedersen Matej Ore šič Source Type: research
Exposure to environmental toxicants is associated with gut microbiome dysbiosis, insulin resistance and obesity
Environ Int. 2024 Mar 20;186:108569. doi: 10.1016/j.envint.2024.108569. Online ahead of print.ABSTRACTEnvironmental toxicants (ETs) are associated with adverse health outcomes. Here we hypothesized that exposures to ETs are linked with obesity and insulin resistance partly through a dysbiotic gut microbiota and changes in the serum levels of secondary bile acids (BAs). Serum BAs, per- and polyfluoroalkyl substances (PFAS) and additional twenty-seven ETs were measured by mass spectrometry in 264 Danes (121 men and 143 women, aged 56.6 ± 7.3 years, BMI 29.7 ± 6.0 kg/m2) using a combination of targeted and suspect screening...
Source: Environment International - March 24, 2024 Category: Environmental Health Authors: Partho Sen Yong Fan Jennifer J Schlezinger Stanislav D Ehrlich Thomas F Webster Tuulia Hy ötyläinen Oluf Pedersen Matej Ore šič Source Type: research
Gastrodin ameliorates depressive-like behaviors via modulating gut microbiota in CUMS-induced mice
CONCLUSION: Our results suggested that GAS improved depressive-like behaviors in mice and impacted the microbial composition of the gut. Our research indicated that dysbiosis of the gut microbiota may be affected by GAS treatment, which improved depressive-like behaviors in the CUMS-induced mouse model of depression.PMID:38521360 | DOI:10.1016/j.bbr.2024.114968 (Source: Behavioural Brain Research)
Source: Behavioural Brain Research - March 23, 2024 Category: Neurology Authors: Yanan Zhao Shaokun Qin Zixian Yang Ye Lu Zongmin Ma Xin Ping Guoqiang Sun Lin Pei Source Type: research
Gastrodin ameliorates depressive-like behaviors via modulating gut microbiota in CUMS-induced mice
CONCLUSION: Our results suggested that GAS improved depressive-like behaviors in mice and impacted the microbial composition of the gut. Our research indicated that dysbiosis of the gut microbiota may be affected by GAS treatment, which improved depressive-like behaviors in the CUMS-induced mouse model of depression.PMID:38521360 | DOI:10.1016/j.bbr.2024.114968 (Source: Brain Research)
Source: Brain Research - March 23, 2024 Category: Neurology Authors: Yanan Zhao Shaokun Qin Zixian Yang Ye Lu Zongmin Ma Xin Ping Guoqiang Sun Lin Pei Source Type: research
Identification of metabolites produced by six gut commensal Bacteroidales strains using non-targeted LC-MS/MS metabolite profiling
Microbiol Res. 2024 Mar 20;283:127700. doi: 10.1016/j.micres.2024.127700. Online ahead of print.ABSTRACTAs the most abundant gram-negative bacterial order in the gastrointestinal tract, Bacteroidales bacteria have been extensively studied for their contribution to various aspects of gut health. These bacteria are renowned for their involvement in immunomodulation and their remarkable capacity to break down complex carbohydrates and fibers. However, the human gut microbiota is known to produce many metabolites that ultimately mediate important microbe-host and microbe-microbe interactions. To gain further insights into the ...
Source: Cell Research - March 22, 2024 Category: Cytology Authors: Maria Victoria Fernandez-Cantos Ambrin Farizah Babu Kati Hanhineva Oscar P Kuipers Source Type: research
Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum
Anim Biotechnol. 2024 Nov;35(1):2331179. doi: 10.1080/10495398.2024.2331179. Epub 2024 Mar 22.ABSTRACTDespite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated ...
Source: Animal Biotechnology - March 22, 2024 Category: Biotechnology Authors: Young-Jun Seo Chiwoong Lim Byeonghwi Lim Jun-Mo Kim Source Type: research
Identification of metabolites produced by six gut commensal Bacteroidales strains using non-targeted LC-MS/MS metabolite profiling
Microbiol Res. 2024 Mar 20;283:127700. doi: 10.1016/j.micres.2024.127700. Online ahead of print.ABSTRACTAs the most abundant gram-negative bacterial order in the gastrointestinal tract, Bacteroidales bacteria have been extensively studied for their contribution to various aspects of gut health. These bacteria are renowned for their involvement in immunomodulation and their remarkable capacity to break down complex carbohydrates and fibers. However, the human gut microbiota is known to produce many metabolites that ultimately mediate important microbe-host and microbe-microbe interactions. To gain further insights into the ...
Source: Cell Research - March 22, 2024 Category: Cytology Authors: Maria Victoria Fernandez-Cantos Ambrin Farizah Babu Kati Hanhineva Oscar P Kuipers Source Type: research
Mendelian randomization analysis reveals a causal effect of Streptococcus salivarius on diabetic retinopathy through regulating host fasting glucose
J Cell Mol Med. 2024 Apr;28(7):e18200. doi: 10.1111/jcmm.18200.ABSTRACTDiabetic retinopathy (DR) is one of leading causes of vision loss in adults with increasing prevalence worldwide. Increasing evidence has emphasized the importance of gut microbiome in the aetiology and development of DR. However, the causal relationship between gut microbes and DR remains largely unknown. To investigate the causal associations of DR with gut microbes and DR risk factors, we employed two-sample Mendelian Randomization (MR) analyses to estimate the causal effects of 207 gut microbes on DR outcomes. Inputs for MR included Genome-wide Asso...
Source: J Cell Mol Med - March 20, 2024 Category: Molecular Biology Authors: Jingjing Li Gongwei Zheng Dingping Jiang Chunyu Deng Yaru Zhang Yunlong Ma Jianzhong Su Source Type: research
Assessment of the Inflammatory Effects of Gut Microbiota from Human Twins Discordant for Ulcerative Colitis on Germ-free Mice
Comp Med. 2024 Mar 20. doi: 10.30802/AALAS-CM-23-000065. Online ahead of print.ABSTRACTDisturbances in gut microbiota are prevalent in inflammatory bowel disease (IBD), which includes ulcerative colitis (UC). However, whether these disturbances contribute to development of the disease or are a result of the disease is unclear. In pairs of human twins discordant for IBD, the healthy twin has a higher risk of developing IBD and a gut microbiota that is more similar to that of IBD patients as compared with healthy individuals. Furthermore, appropriate medical treatment may mitigate these disturbances. To study the correlation...
Source: Comparative Medicine - March 20, 2024 Category: Zoology Authors: Lina A Knudsen Zachariassen Line Sf Mikael L Strube Jesper F Havelund Bartosz Pilecki Anders B Nexoe Frederik T Moller Signe B S ørensen Niels Marcussen Nils J F ærgeman Andre Franke Corinna Bang Uffe Holmskov Axel K Hansen Vibeke Andersen Source Type: research
Toxicity of three microcystin variants on the histology, physiological and metabolism of hepatopancreas and intestinal microbiota of Litopenaeus vannamei
In this study, the shrimp Litopenaeus vannamei were subjected to three different variants of MCs (LR, YR, RR) at a concentration of 1 μg/L each, and elucidated the alterations in both intestinal microbiota and hepatopancreas physiological homeostasis. The results indicate that all three variants of MCs prompted histological alterations in the hepatopancreas, induced elevated levels of oxidative stress biomarkers (H2O2, SOD, and CAT), disturbed the transcription levels of immune-related genes (Crus, ALF, and Lys), along with an increase in apoptotic genes (Casp-3 and P53). Furthermore, the metabolic profiles of the hepatop...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 20, 2024 Category: Biochemistry Authors: Yafei Duan Yuxiu Nan Meng Xiao Yukai Yang Source Type: research
Mendelian randomization analysis reveals a causal effect of Streptococcus salivarius on diabetic retinopathy through regulating host fasting glucose
J Cell Mol Med. 2024 Apr;28(7):e18200. doi: 10.1111/jcmm.18200.ABSTRACTDiabetic retinopathy (DR) is one of leading causes of vision loss in adults with increasing prevalence worldwide. Increasing evidence has emphasized the importance of gut microbiome in the aetiology and development of DR. However, the causal relationship between gut microbes and DR remains largely unknown. To investigate the causal associations of DR with gut microbes and DR risk factors, we employed two-sample Mendelian Randomization (MR) analyses to estimate the causal effects of 207 gut microbes on DR outcomes. Inputs for MR included Genome-wide Asso...
Source: Molecular Medicine - March 20, 2024 Category: Molecular Biology Authors: Jingjing Li Gongwei Zheng Dingping Jiang Chunyu Deng Yaru Zhang Yunlong Ma Jianzhong Su Source Type: research
Assessment of the Inflammatory Effects of Gut Microbiota from Human Twins Discordant for Ulcerative Colitis on Germ-free Mice
Comp Med. 2024 Mar 20. doi: 10.30802/AALAS-CM-23-000065. Online ahead of print.ABSTRACTDisturbances in gut microbiota are prevalent in inflammatory bowel disease (IBD), which includes ulcerative colitis (UC). However, whether these disturbances contribute to development of the disease or are a result of the disease is unclear. In pairs of human twins discordant for IBD, the healthy twin has a higher risk of developing IBD and a gut microbiota that is more similar to that of IBD patients as compared with healthy individuals. Furthermore, appropriate medical treatment may mitigate these disturbances. To study the correlation...
Source: Comparative Medicine - March 20, 2024 Category: Zoology Authors: Lina A Knudsen Zachariassen Line Sf Mikael L Strube Jesper F Havelund Bartosz Pilecki Anders B Nexoe Frederik T Moller Signe B S ørensen Niels Marcussen Nils J F ærgeman Andre Franke Corinna Bang Uffe Holmskov Axel K Hansen Vibeke Andersen Source Type: research
Toxicity of three microcystin variants on the histology, physiological and metabolism of hepatopancreas and intestinal microbiota of Litopenaeus vannamei
In this study, the shrimp Litopenaeus vannamei were subjected to three different variants of MCs (LR, YR, RR) at a concentration of 1 μg/L each, and elucidated the alterations in both intestinal microbiota and hepatopancreas physiological homeostasis. The results indicate that all three variants of MCs prompted histological alterations in the hepatopancreas, induced elevated levels of oxidative stress biomarkers (H2O2, SOD, and CAT), disturbed the transcription levels of immune-related genes (Crus, ALF, and Lys), along with an increase in apoptotic genes (Casp-3 and P53). Furthermore, the metabolic profiles of the hepatop...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 20, 2024 Category: Biochemistry Authors: Yafei Duan Yuxiu Nan Meng Xiao Yukai Yang Source Type: research
Toxicity of three microcystin variants on the histology, physiological and metabolism of hepatopancreas and intestinal microbiota of Litopenaeus vannamei
In this study, the shrimp Litopenaeus vannamei were subjected to three different variants of MCs (LR, YR, RR) at a concentration of 1 μg/L each, and elucidated the alterations in both intestinal microbiota and hepatopancreas physiological homeostasis. The results indicate that all three variants of MCs prompted histological alterations in the hepatopancreas, induced elevated levels of oxidative stress biomarkers (H2O2, SOD, and CAT), disturbed the transcription levels of immune-related genes (Crus, ALF, and Lys), along with an increase in apoptotic genes (Casp-3 and P53). Furthermore, the metabolic profiles of the hepatop...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - March 20, 2024 Category: Biochemistry Authors: Yafei Duan Yuxiu Nan Meng Xiao Yukai Yang Source Type: research
