The supragenic organization of glycoside hydrolase encoding genes reveals distinct strategies for carbohydrate utilization in bacteria
Glycoside hydrolases (GHs) are carbohydrate-active enzymes essential for many environmental (e.g., carbon cycling) and biotechnological (e.g., biofuels) processes. The complete processing of carbohydrates by bacteria requires many enzymes acting synergistically. Here, I investigated the clustered or scattered distribution of 406,337 GH-genes and their association with transporter genes identified in 15,640 completely sequenced bacterial genomes. Different bacterial lineages displayed conserved levels of clustered or scattered GH-genes, but overall, the GH-genes clustering was generally higher than in randomized genomes. In...
Source: Frontiers in Microbiology - June 2, 2023 Category: Microbiology Source Type: research
Study on the Mechanism of Jiaotai Pill Intervention on Insomnia Animal Model Based on Gut Microbiome and Metabolomics
CONCLUSIONS: We hypothesize that JTP may achieve insomnia relief by eliminating inflammation-causing bacteria in the gut and reducing inflammation levels through the brain-gut axis, pointing to potential targets and pathways for future research on JTP.PMID:37260523 | PMC:PMC10229250 | DOI:10.1155/2023/2442505 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - June 1, 2023 Category: Complementary Medicine Authors: Yang Yang Jiao Liu Haosong Ou Xin Ma Jia Li Binghao Shao Ruyi Jin Junyun Zhao Source Type: research
Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study
CONCLUSIONS: - Inter-laboratory agreement by disk diffusion was good or very good. The main challenges were media-related variability for metronidazole and categorical disagreement with the reference method for piperacillin-tazobactam in some cfiA-positive strains. An area of technical uncertainty specific for such strains may be warranted.PMID:37253399 | DOI:10.1016/j.anaerobe.2023.102743 (Source: Anaerobe)
Source: Anaerobe - May 30, 2023 Category: Microbiology Authors: Tore Taksdal Stubhaug Christian G Giske Ulrik S Justesen Gunnar Kahlmeter Erika Matuschek Arnfinn Sundsfjord Dagfinn Skaare Nordic Bacteroides AST Study Group Source Type: research
Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study
CONCLUSIONS: - Inter-laboratory agreement by disk diffusion was good or very good. The main challenges were media-related variability for metronidazole and categorical disagreement with the reference method for piperacillin-tazobactam in some cfiA-positive strains. An area of technical uncertainty specific for such strains may be warranted.PMID:37253399 | DOI:10.1016/j.anaerobe.2023.102743 (Source: Anaerobe)
Source: Anaerobe - May 30, 2023 Category: Microbiology Authors: Tore Taksdal Stubhaug Christian G Giske Ulrik S Justesen Gunnar Kahlmeter Erika Matuschek Arnfinn Sundsfjord Dagfinn Skaare Nordic Bacteroides AST Study Group Source Type: research
Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study
CONCLUSIONS: - Inter-laboratory agreement by disk diffusion was good or very good. The main challenges were media-related variability for metronidazole and categorical disagreement with the reference method for piperacillin-tazobactam in some cfiA-positive strains. An area of technical uncertainty specific for such strains may be warranted.PMID:37253399 | DOI:10.1016/j.anaerobe.2023.102743 (Source: Anaerobe)
Source: Anaerobe - May 30, 2023 Category: Microbiology Authors: Tore Taksdal Stubhaug Christian G Giske Ulrik S Justesen Gunnar Kahlmeter Erika Matuschek Arnfinn Sundsfjord Dagfinn Skaare Nordic Bacteroides AST Study Group Source Type: research
Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study
CONCLUSIONS: - Inter-laboratory agreement by disk diffusion was good or very good. The main challenges were media-related variability for metronidazole and categorical disagreement with the reference method for piperacillin-tazobactam in some cfiA-positive strains. An area of technical uncertainty specific for such strains may be warranted.PMID:37253399 | DOI:10.1016/j.anaerobe.2023.102743 (Source: Anaerobe)
Source: Anaerobe - May 30, 2023 Category: Microbiology Authors: Tore Taksdal Stubhaug Christian G Giske Ulrik S Justesen Gunnar Kahlmeter Erika Matuschek Arnfinn Sundsfjord Dagfinn Skaare Nordic Bacteroides AST Study Group Source Type: research
Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study
CONCLUSIONS: - Inter-laboratory agreement by disk diffusion was good or very good. The main challenges were media-related variability for metronidazole and categorical disagreement with the reference method for piperacillin-tazobactam in some cfiA-positive strains. An area of technical uncertainty specific for such strains may be warranted.PMID:37253399 | DOI:10.1016/j.anaerobe.2023.102743 (Source: Anaerobe)
Source: Anaerobe - May 30, 2023 Category: Microbiology Authors: Tore Taksdal Stubhaug Christian G Giske Ulrik S Justesen Gunnar Kahlmeter Erika Matuschek Arnfinn Sundsfjord Dagfinn Skaare Nordic Bacteroides AST Study Group Source Type: research
Disentangling hindgut metabolism in the American cockroach through single-cell genomics and metatranscriptomics
Omnivorous cockroaches host a complex hindgut microbiota comprised of insect-specific lineages related to those found in mammalian omnivores. Many of these organisms have few cultured representatives, thereby limiting our ability to infer the functional capabilities of these microbes. Here we present a unique reference set of 96 high-quality single cell-amplified genomes (SAGs) from bacterial and archaeal cockroach gut symbionts. We additionally generated cockroach hindgut metagenomic and metatranscriptomic sequence libraries and mapped them to our SAGs. By combining these datasets, we are able to perform an in-depth phylo...
Source: Frontiers in Microbiology - May 30, 2023 Category: Microbiology Source Type: research
Expanding the repertoire of counterselection markers for markerless gene deletion in the human gut bacterium Phocaeicola vulgatus
CONCLUSION: The genetic toolbox for P. vulgatus was expanded by a markerless gene deletion system based on SacB as an efficient counterselection marker. The system was employed to successfully delete three genes in P. vulgatus which all resulted in expected phenotypes as confirmed by subsequent growth experiments.PMID:37244477 | DOI:10.1016/j.anaerobe.2023.102742 (Source: Anaerobe)
Source: Anaerobe - May 27, 2023 Category: Microbiology Authors: Andr é Neff Rebecca L ück Marcel H övels Uwe Deppenmeier Source Type: research
Expanding the repertoire of counterselection markers for markerless gene deletion in the human gut bacterium Phocaeicola vulgatus
CONCLUSION: The genetic toolbox for P. vulgatus was expanded by a markerless gene deletion system based on SacB as an efficient counterselection marker. The system was employed to successfully delete three genes in P. vulgatus which all resulted in expected phenotypes as confirmed by subsequent growth experiments.PMID:37244477 | DOI:10.1016/j.anaerobe.2023.102742 (Source: Anaerobe)
Source: Anaerobe - May 27, 2023 Category: Microbiology Authors: Andr é Neff Rebecca L ück Marcel H övels Uwe Deppenmeier Source Type: research
Expanding the repertoire of counterselection markers for markerless gene deletion in the human gut bacterium Phocaeicola vulgatus
CONCLUSION: The genetic toolbox for P. vulgatus was expanded by a markerless gene deletion system based on SacB as an efficient counterselection marker. The system was employed to successfully delete three genes in P. vulgatus which all resulted in expected phenotypes as confirmed by subsequent growth experiments.PMID:37244477 | DOI:10.1016/j.anaerobe.2023.102742 (Source: Anaerobe)
Source: Anaerobe - May 27, 2023 Category: Microbiology Authors: Andr é Neff Rebecca L ück Marcel H övels Uwe Deppenmeier Source Type: research
Altered gut microbiota profile in patients with perimenopausal panic disorder
This study aimed to discover specific microbiota in PPD patients and the intrinsic connection between them. Gut microbiota was analyzed in PPD patients (n = 40) and healthy controls (n = 40) by 16S rRNA sequencing.ResultsThe results showed reduced α-diversity (richness) in the gut microbiota of PPD patients. β-diversity indicated that PPD and healthy controls had different intestinal microbiota compositions. At the genus level, 30 species of microbiota abundance had significantly different between the PPD and healthy controls. In addition, HAMA, PDSS, and PASS scales were collected in two groups. It was found that Bacter...
Source: Frontiers in Psychiatry - May 25, 2023 Category: Psychiatry Source Type: research
Fecal microbiota transplantation confirmed that 919 Syrup reduced the ratio of erucamide to 5-AVAB in hippocampus to alleviate postpartum depression by regulating gut microbes
ConclusionIn brief, 919 Syrup may downregulate the ratio of hippocampal metabolites erucamide to 5-AVAB by regulating intestinal flora to alleviate postpartum depression, laying a scientific foundation for future pathological research and development of therapeutic drugs for postpartum depression. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - May 24, 2023 Category: Allergy & Immunology Source Type: research
Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway
Conclusion: BDX-01 improved DSS-induced acute colitis by regulating intestinal BSH activity and the FXR-NLRP3 signaling pathway. Our findings indicate that BDX-01 is a promising probiotic to improve the management of ulcerative colitis. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - May 24, 2023 Category: Drugs & Pharmacology Source Type: research
Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
This study analyzed the microbiota of the gut, semen, and urine in individuals with semen abnormalities to identify potential probiotics and pathogenic bacteria that affect semen parameters and help develop new methods for the diagnosis and treatment of patients with semen abnormalities.MethodsWe recruited 12 individuals with normal semen parameters (control group), 12 with asthenospermia but no semen hyperviscosity (Group_1), 6 with oligospermia (Group_2), 9 with severe oligospermia or azoospermia (Group_3), and 14 with semen hyperviscosity only (Group_4). The semen, gut, and urine microbiota were examined by analyzing th...
Source: Frontiers in Microbiology - May 24, 2023 Category: Microbiology Source Type: research
