Phenotypic drug-susceptibility profiles and genetic analysis based on whole-genome sequencing of < i > Mycobacterium avium < /i > complex isolates in Thailand
This study aimed to determine the drug-susceptibility profiles of MAC clinical isolates and to investigate the genetic basis conferring drug resistance using whole-genome sequencing (WGS) analysis. Drug-susceptibility profiles based on minimum inhibitory concentration (MIC) assays were determined for 38 MAC clinical isolates (12Mycobacterium avium and 26Mycobacterium intracellulare). Mutations associated with drug resistance were identified through genome analysis of these isolates, and their phylogenetic relationships were also examined. Drug resistance, based on MIC values, was most commonly observed for moxifloxacin (81...
Source: PLoS One - November 22, 2023 Category: Biomedical Science Authors: Auttawit Sirichoat Source Type: research
Short oral regimens for pulmonary rifampicin-resistant tuberculosis (TB-PRACTECAL): an open-label, randomised, controlled, phase 2B-3, multi-arm, multicentre, non-inferiority trial
Lancet Respir Med. 2023 Nov 15:S2213-2600(23)00389-2. doi: 10.1016/S2213-2600(23)00389-2. Online ahead of print.ABSTRACTBACKGROUND: Around 500 000 people worldwide develop rifampicin-resistant tuberculosis each year. The proportion of successful treatment outcomes remains low and new treatments are needed. Following an interim analysis, we report the final safety and efficacy outcomes of the TB-PRACTECAL trial, evaluating the safety and efficacy of oral regimens for the treatment of rifampicin-resistant tuberculosis.METHODS: This open-label, randomised, controlled, multi-arm, multicentre, non-inferiority trial was conducte...
Source: Respiratory Care - November 19, 2023 Category: Respiratory Medicine Authors: Bern-Thomas Nyang'wa Catherine Berry Emil Kazounis Ilaria Motta Nargiza Parpieva Zinaida Tigay Ronelle Moodliar Matthew Dodd Varvara Solodovnikova Irina Liverko Shakira Rajaram Mohammed Rassool Timothy McHugh Melvin Spigelman David A Moore Koert Ritmeijer Source Type: research
Diagnostic efficacy of an optimized nucleotide MALDI-TOF –MS assay for anti-tuberculosis drug resistance detection
ConclusionThe nucleotide MALDI-TOF –MS assay yielded highly consistent results compared to DST and WGS, suggesting that it is a promising tool for the rapid detection of sensitivity to RIF, INH, EMB, and MXF. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 18, 2023 Category: Microbiology Source Type: research
A case of chronic bacterial prostatitis due to Mycoplasma genitalium
We describe the case of a 42-year-old man with recurrent urinary tract infections since 2018 who developed chronic prostatitis despite several and prolonged antibiotic courses. Multiparametric prostatic magnetic resonance showed peripheral inflammatory alterations. A 4-glass Meares-Stamey test detected MG in the third voided bladder (VB3) sample. Moxifloxacin 400 mg daily for 28 days resulted in sustained clinical and microbiological cure. (Source: Sexually Transmitted Infections)
Source: Sexually Transmitted Infections - November 17, 2023 Category: Sexual Medicine Authors: Rossotti, R., Travi, G., Bana, N. B., Orcese, C., Puoti, M. Tags: Case reports Source Type: research
< em > Mycoplasma phocimorsus < /em > sp. nov., isolated from Scandinavian patients with seal finger or septic arthritis after contact with seals
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006163.ABSTRACTSeal finger (sealer's finger, spekk finger), an extremely painful hand infection contracted by individuals handling seals, has previously been associated with Mycoplasma phocacerebrale. From 2000 to 2014, six independent strains of a novel Mycoplasma species were isolated at Statens Serum Institut, Denmark, from Scandinavian patients with seal finger (M5725T, M6447, M6620, M6642 and M6879) or septic arthritis (M6921). Prior to the onset of infection, all patients had reported contact with unspeciated seals. All isolates grew within 2-5 days in ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2023 Category: Microbiology Authors: Axel Skafte-Holm Thomas Roland Pedersen Maria Fr ølund Marc Stegger Klaus Qvortrup Dina L Michaels Daniel R Brown Jorgen Skov Jensen Source Type: research
< em > Mycoplasma phocimorsus < /em > sp. nov., isolated from Scandinavian patients with seal finger or septic arthritis after contact with seals
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006163.ABSTRACTSeal finger (sealer's finger, spekk finger), an extremely painful hand infection contracted by individuals handling seals, has previously been associated with Mycoplasma phocacerebrale. From 2000 to 2014, six independent strains of a novel Mycoplasma species were isolated at Statens Serum Institut, Denmark, from Scandinavian patients with seal finger (M5725T, M6447, M6620, M6642 and M6879) or septic arthritis (M6921). Prior to the onset of infection, all patients had reported contact with unspeciated seals. All isolates grew within 2-5 days in ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2023 Category: Microbiology Authors: Axel Skafte-Holm Thomas Roland Pedersen Maria Fr ølund Marc Stegger Klaus Qvortrup Dina L Michaels Daniel R Brown Jorgen Skov Jensen Source Type: research
< em > Mycoplasma phocimorsus < /em > sp. nov., isolated from Scandinavian patients with seal finger or septic arthritis after contact with seals
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006163.ABSTRACTSeal finger (sealer's finger, spekk finger), an extremely painful hand infection contracted by individuals handling seals, has previously been associated with Mycoplasma phocacerebrale. From 2000 to 2014, six independent strains of a novel Mycoplasma species were isolated at Statens Serum Institut, Denmark, from Scandinavian patients with seal finger (M5725T, M6447, M6620, M6642 and M6879) or septic arthritis (M6921). Prior to the onset of infection, all patients had reported contact with unspeciated seals. All isolates grew within 2-5 days in ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2023 Category: Microbiology Authors: Axel Skafte-Holm Thomas Roland Pedersen Maria Fr ølund Marc Stegger Klaus Qvortrup Dina L Michaels Daniel R Brown Jorgen Skov Jensen Source Type: research
Effects of the HIV ‐1 maturation inhibitor GSK3640254 on QT interval in healthy participants
Model-predicted ΔΔQTc interval at geometric mean GSK'254 concentrations associated with 100- and 500-mg doses. AbstractGSK3640254 (GSK'254) is a novel HIV-1 maturation inhibitor with pharmacokinetics supporting once-daily (QD) therapy for HIV-1 treatment. This thorough QT/corrected QT (QTc) study evaluated the effect of GSK'254 on cardiac repolarization. In this two-part, randomized study, healthy participants received GSK'254 or placebo QD for 7 days (part 1) to determine safety and pharmacokinetics of a 500-mg supratherapeutic dose. Four sequential treatment periods composed the main QTc study (part 2): GSK'254 100...
Source: Pharmacology Research and Perspectives - November 15, 2023 Category: Drugs & Pharmacology Authors: Ying Zhang,
Mark Bush,
Parto Yazdani,
Joyce Zhan,
Bo Wen,
Veronica Bainbridge,
Brian R. Wynne,
Samit Joshi,
Max Lataillade Tags: ORIGINAL ARTICLE Source Type: research
A Review of Omadacycline for Potential Utility in the Military Health System for the Treatment of Wound Infections
CONCLUSIONS: Omadacycline has broad-spectrum activity, the option to be orally administered and an established safety profile, making it a potentially attractive replacement for moxifloxacin in the military individual first aid kit, especially when accounting for the increasing resistance to fluoroquinolones. Further studies and clinical evaluation are warranted to support broader use of omadacycline to treat combat wound infections in the military healthcare system.PMID:37963013 | DOI:10.1093/milmed/usad417 (Source: Military Medicine)
Source: Military Medicine - November 14, 2023 Category: International Medicine & Public Health Authors: Daniel V Zurawski Alisa W Serio Chad Black Brandon Pybus Kevin S Akers Daniel H Deck Sheila Johnson Supaksorn Chattagul Schroeder M Noble Malik Raynor Charlotte A Lanteri Source Type: research
Structural modeling of hERG channel –drug interactions using Rosetta
The human ether-a-go-go-related gene (hERG) not only encodes a potassium-selective voltage-gated ion channel essential for normal electrical activity in the heart but is also a major drug anti-target. Genetic hERG mutations and blockage of the channel pore by drugs can cause long QT syndrome, which predisposes individuals to potentially deadly arrhythmias. However, not all hERG-blocking drugs are proarrhythmic, and their differential affinities to discrete channel conformational states have been suggested to contribute to arrhythmogenicity. We used Rosetta electron density refinement and homology modeling to build structur...
Source: Frontiers in Pharmacology - November 14, 2023 Category: Drugs & Pharmacology Source Type: research
Drug-ionic surfactant interactions: density, sound speed, spectroscopic, and electrochemical studies
Eur Biophys J. 2023 Nov 9. doi: 10.1007/s00249-023-01689-2. Online ahead of print.ABSTRACTThe failure of antibiotics against infectious diseases has become a global health issue due to the incessant use of antibiotics in the community and a lack of entry of new antibacterial drugs onto the market. The limited knowledge of biophysical interactions of existing antibiotics with bio-membranes is one of the major hurdles to design and develop more effective antibiotics. Surfactant systems are the simplest biological membrane models that not only mimic the cell membrane functions but are also used to investigate the biophysical ...
Source: European Biophysics Journal : EBJ - November 9, 2023 Category: Physics Authors: Muhammad Sohail Hafiz Muhammad Abd Ur Rahman Muhammad Nadeem Asghar Source Type: research
Drug-ionic surfactant interactions: density, sound speed, spectroscopic, and electrochemical studies
Eur Biophys J. 2023 Nov 9. doi: 10.1007/s00249-023-01689-2. Online ahead of print.ABSTRACTThe failure of antibiotics against infectious diseases has become a global health issue due to the incessant use of antibiotics in the community and a lack of entry of new antibacterial drugs onto the market. The limited knowledge of biophysical interactions of existing antibiotics with bio-membranes is one of the major hurdles to design and develop more effective antibiotics. Surfactant systems are the simplest biological membrane models that not only mimic the cell membrane functions but are also used to investigate the biophysical ...
Source: European Biophysics Journal : EBJ - November 9, 2023 Category: Physics Authors: Muhammad Sohail Hafiz Muhammad Abd Ur Rahman Muhammad Nadeem Asghar Source Type: research
Drug-ionic surfactant interactions: density, sound speed, spectroscopic, and electrochemical studies
Eur Biophys J. 2023 Nov 9. doi: 10.1007/s00249-023-01689-2. Online ahead of print.ABSTRACTThe failure of antibiotics against infectious diseases has become a global health issue due to the incessant use of antibiotics in the community and a lack of entry of new antibacterial drugs onto the market. The limited knowledge of biophysical interactions of existing antibiotics with bio-membranes is one of the major hurdles to design and develop more effective antibiotics. Surfactant systems are the simplest biological membrane models that not only mimic the cell membrane functions but are also used to investigate the biophysical ...
Source: European Biophysics Journal : EBJ - November 9, 2023 Category: Physics Authors: Muhammad Sohail Hafiz Muhammad Abd Ur Rahman Muhammad Nadeem Asghar Source Type: research
Drug-ionic surfactant interactions: density, sound speed, spectroscopic, and electrochemical studies
Eur Biophys J. 2023 Nov 9. doi: 10.1007/s00249-023-01689-2. Online ahead of print.ABSTRACTThe failure of antibiotics against infectious diseases has become a global health issue due to the incessant use of antibiotics in the community and a lack of entry of new antibacterial drugs onto the market. The limited knowledge of biophysical interactions of existing antibiotics with bio-membranes is one of the major hurdles to design and develop more effective antibiotics. Surfactant systems are the simplest biological membrane models that not only mimic the cell membrane functions but are also used to investigate the biophysical ...
Source: European Biophysics Journal : EBJ - November 9, 2023 Category: Physics Authors: Muhammad Sohail Hafiz Muhammad Abd Ur Rahman Muhammad Nadeem Asghar Source Type: research
