Dose-response technique combined with stable isotope tracing for drug metabolite profiling by using high-resolution mass spectrometry
Conclusion: The results reveal that the proposed approach can effectively and comprehensively identify ROS metabolites. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - December 13, 2023 Category: Drugs & Pharmacology Source Type: research
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivo and in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed...
Source: Chemico-Biological Interactions - December 6, 2023 Category: Molecular Biology Authors: Siyuan Wang Xuqing Li Jun Li Aiping Wang Fangfang Li Huiqing Hu Tengfei Long Xueting Pei Hongyan Li Fei Zhong Fengyu Zhu Source Type: research
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivo and in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed...
Source: Chemico-Biological Interactions - December 6, 2023 Category: Molecular Biology Authors: Siyuan Wang Xuqing Li Jun Li Aiping Wang Fangfang Li Huiqing Hu Tengfei Long Xueting Pei Hongyan Li Fei Zhong Fengyu Zhu Source Type: research
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivo and in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed...
Source: Chemico-Biological Interactions - December 6, 2023 Category: Molecular Biology Authors: Siyuan Wang Xuqing Li Jun Li Aiping Wang Fangfang Li Huiqing Hu Tengfei Long Xueting Pei Hongyan Li Fei Zhong Fengyu Zhu Source Type: research
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivo and in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed...
Source: Chemico-Biological Interactions - December 6, 2023 Category: Molecular Biology Authors: Siyuan Wang Xuqing Li Jun Li Aiping Wang Fangfang Li Huiqing Hu Tengfei Long Xueting Pei Hongyan Li Fei Zhong Fengyu Zhu Source Type: research
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivo and in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed...
Source: Chemico-Biological Interactions - December 6, 2023 Category: Molecular Biology Authors: Siyuan Wang Xuqing Li Jun Li Aiping Wang Fangfang Li Huiqing Hu Tengfei Long Xueting Pei Hongyan Li Fei Zhong Fengyu Zhu Source Type: research
Environmental chemicals change extracellular lipidome of mature human white adipocytes
This study points to early signs of metabolic disruption and likely systemic effects of mature adipocyte exposure to environmental chemicals, as well as to the need to include lipidomic endpoints in the assessment of adverse effects of MDCs.PMID:38048832 | DOI:10.1016/j.chemosphere.2023.140852 (Source: Chemosphere)
Source: Chemosphere - December 4, 2023 Category: Chemistry Authors: Paula Burkhardt Susana Alejandra Palma-Duran AstrudR R Tuck Kalle Norgren Xinyi Li Violetta Nikiforova Julian L Griffin Vesna Munic Kos Source Type: research
Environmental chemicals change extracellular lipidome of mature human white adipocytes
This study points to early signs of metabolic disruption and likely systemic effects of mature adipocyte exposure to environmental chemicals, as well as to the need to include lipidomic endpoints in the assessment of adverse effects of MDCs.PMID:38048832 | DOI:10.1016/j.chemosphere.2023.140852 (Source: Chemosphere)
Source: Chemosphere - December 4, 2023 Category: Chemistry Authors: Paula Burkhardt Susana Alejandra Palma-Duran AstrudR R Tuck Kalle Norgren Xinyi Li Violetta Nikiforova Julian L Griffin Vesna Munic Kos Source Type: research
Environmental chemicals change extracellular lipidome of mature human white adipocytes
This study points to early signs of metabolic disruption and likely systemic effects of mature adipocyte exposure to environmental chemicals, as well as to the need to include lipidomic endpoints in the assessment of adverse effects of MDCs.PMID:38048832 | DOI:10.1016/j.chemosphere.2023.140852 (Source: Chemosphere)
Source: Chemosphere - December 4, 2023 Category: Chemistry Authors: Paula Burkhardt Susana Alejandra Palma-Duran AstrudR R Tuck Kalle Norgren Xinyi Li Violetta Nikiforova Julian L Griffin Vesna Munic Kos Source Type: research
Environmental chemicals change extracellular lipidome of mature human white adipocytes
This study points to early signs of metabolic disruption and likely systemic effects of mature adipocyte exposure to environmental chemicals, as well as to the need to include lipidomic endpoints in the assessment of adverse effects of MDCs.PMID:38048832 | DOI:10.1016/j.chemosphere.2023.140852 (Source: Chemosphere)
Source: Chemosphere - December 4, 2023 Category: Chemistry Authors: Paula Burkhardt Susana Alejandra Palma-Duran Astrud R R Tuck Kalle Norgren Xinyi Li Violetta Nikiforova Julian L Griffin Vesna Munic Kos Source Type: research
PPARG dysregulation as a potential molecular target in adrenal Cushing's syndrome
BackgroundWe performed a transcriptomic analysis of adrenal signaling pathways in various forms of endogenous Cushing’s syndrome (CS) to define areas of dysregulated and druggable targets.MethodologyNext-generation sequencing was performed on adrenal samples of patients with primary bilateral macronodular adrenal hyperplasia (PBMAH, n=10) and control adrenal samples (n=8). The validation groups included cortisol-producing adenoma (CPA, n=9) and samples from patients undergoing bilateral adrenalectomy for Cushing’s disease (BADX-CD, n=8). In vivo findings were further characterized using three adrenocortical cell-lines ...
Source: Frontiers in Endocrinology - November 30, 2023 Category: Endocrinology Source Type: research
Peroxisome Proliferator-Activated Receptor < em > γ < /em > Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy
In this study, we used PPARγ agonist rosiglitazone and inhibitor GW9662, as well as autophagy activator rapamycin and inhibitor 3-methyladenine, to unravel the probable mechanism of PPARγ engaged in lipid metabolism in sheep trophoblast cells (STCs). After 12 h, 24 h, and 48 h of drug treatment, the levels of autophagy-related proteins were detected by Western blot, the triglyceride content and MDA level of cells were detected by colorimetry, and the lipid droplets and lysosomes were localized by immunofluorescence. We found that PPARγ inhibited the activity of mammalian target of rapamycin (mTOR) pathway in STCs for a ...
Source: PPAR Research - November 29, 2023 Category: Genetics & Stem Cells Authors: Kexing Hao Jing Wang Hengbin Yu Lei Chen Weibin Zeng Zhengrong Wang Guangdong Hu Source Type: research
