A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala.

A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala.
J Inorg Biochem. 2015 Mar 24;
Authors: Kajikawa T, Sugiyama R, Kataoka K, Sakurai T
Abstract
A multicopper oxidase, CueO was doubly mutated at its type I copper ligand, Cys500 and an acidic amino acid residue located in the proton transfer pathway, Glu506, to Ser and Ala, respectively. Cys500Ser/Glu506Ala was mainly in a novel resting form to afford the absorption band at ca. 400nm and an EPR signal with a highly anisotropic character derived from type III copper. However, Cys500Ser/Glu506Ala gave the same reaction intermediate (peroxide intermediate) as that from Cys500Ser and Cys500Ser/Glu506Gln.
PMID: 25840508 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - March 24, 2015 Category: Biochemistry Authors: Kajikawa T, Sugiyama R, Kataoka K, Sakurai T Tags: J Inorg Biochem Source Type: research