Upregulation of biochemical and biophysical properties of cell-laden microfiber, silk-hyaluronic acid composite

We present a binary hybrid silk and hyaluronic acid hydrogel microfiber generated through a microfluidic system to encapsulate cells with superior mechanical properties and biocompatibility. Cell-laden hydrogel microfibers were continuously produced through coaxial double orifice microfluidic device and horseradish peroxidase mediated crosslinking, which conjugated introduce phenolic moieties in the backbone of silk fibroin and HA derivatives (Silk-Ph and HA-Ph, respectively). The iterative hybrid Silk-Ph + HA-Ph fibers were fabricated in tunable size distribution between 195 and 680 μm through control of outer flow velocity. Tensile strength and maximum stain of prepared Silk-Ph + HA-Ph sample upregulated more than three times higher than the single HA-Ph sample, which demonstrated significant impacts of synthesized silk derivative in hydrogel fiber composition. The proteolytic degradation of microfibers manipulated by hyaluronidase and collagenase treatment. Encapsulation process and crosslinking did not insert any harmful effect on cell viability (> 90%) and the cells maintained their growth ability after encapsulation process. Cellular filament-like tissue fabricated from proliferation of cells in Silk-Ph + HA-Ph microfiber.PMID:35588975 | DOI:10.1016/j.ijbiomac.2022.05.080

Source: International Journal of Biological Macromolecules - May 19, 2022 Category: Biochemistry Authors: Seyed Ali Fatahian Alireza Motavalizadehkakhky Malihesadat Hosseiny Seyed Mohammad Mahdi Nouri Rahele Zhiani Mojtaba Sohrabpour Mehdi Khanmohammadi Source Type: research